Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	841	3p21.1	NEK4	NIMA related kinase 4
Mouse	-	792	14 B	Nek4	NIMA (never in mitosis gene a)-related expressed kinase 4
Rat	-	793	16p16	Nek4	NIMA-related kinase 4

Previous and Unofficial Names

NRK2 | serine/threonine protein kinase-2 | STK2 | NIMA-related kinase 4

Database Links
Alphafold	P51957 (Hs), Q9Z1J2 (Mm)
BRENDA	2.7.11.1
ChEMBL Target	CHEMBL5819 (Hs)
Ensembl Gene	ENSG00000114904 (Hs), ENSMUSG00000021918 (Mm), ENSRNOG00000017997 (Rn)
Entrez Gene	6787 (Hs), 23955 (Mm), 306252 (Rn)
Human Protein Atlas	ENSG00000114904 (Hs)
KEGG Enzyme	2.7.11.1
KEGG Gene	hsa:6787 (Hs), mmu:23955 (Mm), rno:306252 (Rn)
OMIM	601959 (Hs)
Pharos	P51957 (Hs)
RefSeq Nucleotide	NM_001193533 (Hs), NM_011849 (Mm), NM_001013134 (Rn)
RefSeq Protein	NP_003148 (Hs), NP_035979 (Mm), NP_001013152 (Rn)
UniProtKB	P51957 (Hs), Q9Z1J2 (Mm)
Wikipedia	NEK4 (Hs)

Enzyme Reaction
EC Number: 2.7.11.1

DiscoveRx KINOMEscan® screen
A screen of 72 inhibitors against 456 human kinases. Quantitative data were derived using DiscoveRx KINOMEscan® platform. http://www.discoverx.com/services/drug-discovery-development-services/kinase-profiling/kinomescan Reference: 2-3
Key to terms and symbols		Click column headers to sort
Target used in screen: NEK4
Ligand Sp. Type Action Value Parameter AST-487 Hs Inhibitor Inhibition 6.3 pKd KW-2449 Hs Inhibitor Inhibition 6.0 pKd fedratinib Hs Inhibitor Inhibition 5.8 pKd tamatinib Hs Inhibitor Inhibition 5.7 pKd dovitinib Hs Inhibitor Inhibition 5.6 pKd SB203580 Hs Inhibitor Inhibition <5.5 pKd ruboxistaurin Hs Inhibitor Inhibition <5.5 pKd erlotinib Hs Inhibitor Inhibition <5.5 pKd linifanib Hs Inhibitor Inhibition <5.5 pKd GSK690693 Hs Inhibitor Inhibition <5.5 pKd
Displaying the top 10 most potent ligands  View all ligands in screen »

EMD Millipore KinaseProfilerTM screen/Reaction Biology Kinase HotspotSM screen
A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfilerTM service. A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase HotspotSM platform. http://www.millipore.com/techpublications/tech1/pf3036 http://www.reactionbiology.com/webapps/main/pages/kinase.aspx Reference: ...1
Key to terms and symbols		Click column headers to sort
Target used in screen: nd/NEK4
Ligand Sp. Type Action % Activity remaining at 0.5µM % Activity remaining at 1µM % Activity remaining at 10µM K-252a Hs Inhibitor Inhibition 15.0 SB 218078 Hs Inhibitor Inhibition 22.0 JAK3 inhibitor VI Hs Inhibitor Inhibition 26.1 bosutinib Hs Inhibitor Inhibition 32.6 PKR inhibitor Hs Inhibitor Inhibition 39.5 staurosporine Hs Inhibitor Inhibition 46.8 Gö 6976 Hs Inhibitor Inhibition 49.1 casein kinase II inhibitor III Hs Inhibitor Inhibition 54.6 STO609 Hs Inhibitor Inhibition 60.4 Cdk1/2 inhibitor III Hs Inhibitor Inhibition 60.5
Displaying the top 10 most potent ligands  View all ligands in screen »