borrelidin   Click here for help

GtoPdb Ligand ID: 11306

Synonyms: (-)-borrelidin
Antimalarial Ligand
Comment: Borrelidin is a macrolide antibiotic originally isolated from Streptomyces. It is a noncompetitive inhibitor of both bacterial and eukaryotic threonyl-tRNA synthetases (also known as threonine--tRNA ligase), exhibiting antibacterial, antimalarial and antiangiogenic activities.

The Malaria tab on this ligand page provides additional curator comments of relevance to the Guide to MALARIA PHARMACOLOGY
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2D Structure
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Physico-chemical Properties
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Hydrogen bond acceptors 7
Hydrogen bond donors 3
Rotatable bonds 2
Topological polar surface area 127.85
Molecular weight 489.31
XLogP 4.67
No. Lipinski's rules broken 0
SMILES / InChI / InChIKey
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Canonical SMILES N#C/C/1=C/C=C/C[C@H](OC(=O)C[C@@H]([C@H](C[C@H](C[C@H](C[C@@H]([C@H]1O)C)C)C)C)O)[C@@H]1CCC[C@H]1C(=O)O
Isomeric SMILES N#C/C/1=C/C=C/C[C@H](OC(=O)C[C@@H]([C@H](C[C@H](C[C@H](C[C@@H]([C@H]1O)C)C)C)C)O)[C@@H]1CCC[C@H]1C(=O)O
InChI InChI=1S/C28H43NO6/c1-17-12-18(2)14-20(4)27(32)21(16-29)8-5-6-11-25(22-9-7-10-23(22)28(33)34)35-26(31)15-24(30)19(3)13-17/h5-6,8,17-20,22-25,27,30,32H,7,9-15H2,1-4H3,(H,33,34)/b6-5+,21-8-/t17-,18+,19-,20-,22+,23+,24-,25-,27+/m0/s1
InChI Key OJCKRNPLOZHAOU-UGKRXNSESA-N
Bioactivity Comments
Borrelidin is toxic at low concentrations in human cell culture (HEK293 cells, IC50 of 345 nM), and it thought to inhibit human threonyl-tRNA synthetase 1 (TARS1)[1].
In P. falciparum, borrelidin acts rapidly and has potent asexual blood stage activity against both drug-sensitive and drug-resistant strains of the parasite.
Whole organism assay data
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MOA/likely target Sp. Assay description Type Action Value Parameter Concentration range (M) Reference
Plasmodium falciparum threonyl-tRNA synthetase Pf3D7 Parasite growth inhibition assay - - 8.9 – 9.0 pIC50 - 1
pIC50 9.0 (IC50 9.7x10-10 M) Parasite lactate dehydrogenase (pLDH ) assay following 96 hours in culture [1]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
pIC50 8.9 (IC50 1.24x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay following 48 hours in culture [1]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Plasmodium falciparum threonyl-tRNA synthetase PfFCR3 Parasite growth inhibition assay - - 8.7 pIC50 - 2
pIC50 8.7 (IC50 1.8x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay [2]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)
Plasmodium falciparum threonyl-tRNA synthetase PfK1 Parasite growth inhibition assay - - 8.7 pIC50 - 2
pIC50 8.7 (IC50 1.9x10-9 M) Parasite lactate dehydrogenase (pLDH ) assay. [2]
Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)