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PLCγ2

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Target id: 1408

Nomenclature: PLCγ2

Family: Phosphoinositide-specific phospholipase C

Contents:

Gene and Protein Information Click here for help
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human - 1265 16q24.1 PLCG2 phospholipase C gamma 2
Mouse - 1265 8 64.26 cM Plcg2 phospholipase C, gamma 2
Rat - 1265 19q12 Plcg2 phospholipase C
Previous and Unofficial Names Click here for help
PLC-IV | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 | phosphoinositide phospholipase C-gamma-2 | phospholipase C, gamma 2 | phospholipase C-IV | PLCgamma2 | phospholipase C, gamma 2 (phosphatidylinositol-specific) | phospholipase C
Database Links Click here for help
Alphafold P16885 (Hs), Q8CIH5 (Mm), P24135 (Rn)
BRENDA 3.1.4.11
CATH/Gene3D 2.60.40.150, 3.20.20.190, 2.30.29.30
ChEMBL Target CHEMBL4100 (Hs), CHEMBL3608199 (Mm), CHEMBL5230 (Rn)
Ensembl Gene ENSG00000197943 (Hs), ENSMUSG00000034330 (Mm), ENSRNOG00000051986 (Rn)
Entrez Gene 5336 (Hs), 234779 (Mm), 29337 (Rn)
Human Protein Atlas ENSG00000197943 (Hs)
KEGG Enzyme 3.1.4.11
KEGG Gene hsa:5336 (Hs), mmu:234779 (Mm), rno:29337 (Rn)
OMIM 600220 (Hs)
Pharos P16885 (Hs)
RefSeq Nucleotide NM_002661 (Hs)
RefSeq Protein NP_002652 (Hs)
UniProtKB P16885 (Hs), Q8CIH5 (Mm), P24135 (Rn)
Wikipedia PLCG2 (Hs)
Enzyme Reaction Click here for help
EC Number: 3.1.4.11 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Cofactors Click here for help
Cofactor Species Comments Reference
Ca2+ Human
Endogenous activators (Human)
PIP3, Rac1 (RAC1, P63000), Rac2 (RAC2, P15153), Rac3 (RAC3, P60763)  [1,3,7]

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Inhibitors
Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
CCT129957 Small molecule or natural product Hs Inhibition 5.5 pIC50 4
⤷ pIC50 5.5 (IC50 3x10-6 M) [4]
Description: Average IC50 (n=3)
Immunopharmacology Comments
Two structurally similar isoforms of PLCγ are expressed by mast cells (PLCγ1 and PLCγ2). Both are important enzymes in the integrated signalling pathways leading to mast cell activation [2,5], although PLCγ1 may be the predominant isoform active in human mast cells [6].
Cell Type Associations
Immuno Cell Type:  Mast cells
Cell Ontology Term:   mast cell (CL:0000097)
Immuno Process Associations
Immuno Process:  Inflammation
GO Annotations:  Associated to 7 GO processes
GO:0002223 stimulatory C-type lectin receptor signaling pathway ISS
GO:0002224 toll-like receptor signaling pathway ISS
GO:0002281 macrophage activation involved in immune response ISS
GO:0060100 positive regulation of phagocytosis, engulfment IMP
GO:0061760 antifungal innate immune response ISS
GO:0150078 positive regulation of neuroinflammatory response IMP
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GO:0002316 follicular B cell differentiation IEA
Immuno Process:  Antigen presentation
GO Annotations:  Associated to 2 GO processes
GO:0060100 positive regulation of phagocytosis, engulfment IMP
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GO:0002092 positive regulation of receptor internalization IEA
Immuno Process:  B cell (activation)
GO Annotations:  Associated to 2 GO processes
GO:0030183 B cell differentiation ISS
click arrow to show/hide IEA associations
GO:0002316 follicular B cell differentiation IEA
Immuno Process:  Immune regulation
GO Annotations:  Associated to 8 GO processes
GO:0002223 stimulatory C-type lectin receptor signaling pathway ISS
GO:0002224 toll-like receptor signaling pathway ISS
GO:0002732 positive regulation of dendritic cell cytokine production ISS
GO:0038095 Fc-epsilon receptor signaling pathway TAS
GO:0050852 T cell receptor signaling pathway ISS
GO:0050853 B cell receptor signaling pathway IDA
GO:0060907 positive regulation of macrophage cytokine production ISS
GO:0150078 positive regulation of neuroinflammatory response IMP
Immuno Process:  Immune system development
GO Annotations:  Associated to 2 GO processes
GO:0030183 B cell differentiation ISS
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GO:0002316 follicular B cell differentiation IEA
Immuno Process:  Cellular signalling
GO Annotations:  Associated to 8 GO processes
GO:0002223 stimulatory C-type lectin receptor signaling pathway ISS
GO:0002224 toll-like receptor signaling pathway ISS
GO:0002281 macrophage activation involved in immune response ISS
GO:0030183 B cell differentiation ISS
GO:0038095 Fc-epsilon receptor signaling pathway TAS
GO:0050852 T cell receptor signaling pathway ISS
GO:0050853 B cell receptor signaling pathway IDA
click arrow to show/hide IEA associations
GO:0002316 follicular B cell differentiation IEA
Immuno Process:  Cytokine production & signalling
GO Annotations:  Associated to 9 GO processes, IEA only
GO:0002732 positive regulation of dendritic cell cytokine production ISS
GO:0032733 positive regulation of interleukin-10 production ISS
GO:0032735 positive regulation of interleukin-12 production ISS
GO:0032743 positive regulation of interleukin-2 production ISS
GO:0032747 positive regulation of interleukin-23 production ISS
GO:0032755 positive regulation of interleukin-6 production ISS
GO:0032760 positive regulation of tumor necrosis factor production ISS
GO:0060907 positive regulation of macrophage cytokine production ISS
click arrow to show/hide IEA associations
GO:0032481 positive regulation of type I interferon production IEA

References

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1. Bae YS, Cantley LG, Chen CS, Kim SR, Kwon KS, Rhee SG. (1998) Activation of phospholipase C-gamma by phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem, 273 (8): 4465-9. [PMID:9468499]

2. Gilfillan AM, Tkaczyk C. (2006) Integrated signalling pathways for mast-cell activation. Nat Rev Immunol, 6 (3): 218-30. [PMID:16470226]

3. Piechulek T, Rehlen T, Walliser C, Vatter P, Moepps B, Gierschik P. (2005) Isozyme-specific stimulation of phospholipase C-gamma2 by Rac GTPases. J Biol Chem, 280 (47): 38923-31. [PMID:16172125]

4. Reynisson J, Court W, O'Neill C, Day J, Patterson L, McDonald E, Workman P, Katan M, Eccles SA. (2009) The identification of novel PLC-gamma inhibitors using virtual high throughput screening. Bioorg Med Chem, 17 (8): 3169-76. [PMID:19303309]

5. Saitoh S, Arudchandran R, Manetz TS, Zhang W, Sommers CL, Love PE, Rivera J, Samelson LE. (2000) LAT is essential for Fc(epsilon)RI-mediated mast cell activation. Immunity, 12 (5): 525-35. [PMID:10843385]

6. Tkaczyk C, Beaven MA, Brachman SM, Metcalfe DD, Gilfillan AM. (2003) The phospholipase C gamma 1-dependent pathway of Fc epsilon RI-mediated mast cell activation is regulated independently of phosphatidylinositol 3-kinase. J Biol Chem, 278 (48): 48474-84. [PMID:13129935]

7. Walliser C, Retlich M, Harris R, Everett KL, Josephs MB, Vatter P, Esposito D, Driscoll PC, Katan M, Gierschik P et al.. (2008) rac regulates its effector phospholipase Cgamma2 through interaction with a split pleckstrin homology domain. J Biol Chem, 283 (44): 30351-62. [PMID:18728011]

How to cite this page

Phosphoinositide-specific phospholipase C: PLCγ2. Last modified on 14/02/2017. Accessed on 27/09/2023. IUPHAR/BPS Guide to PHARMACOLOGY, https://www.guidetomalariapharmacology.org/GRAC/ObjectDisplayForward?objectId=1408.