GNF4877   Click here for help

GtoPdb Ligand ID: 10688

Synonyms: GNF-4877
Compound class: Synthetic organic
Comment: GNF4877 is a dual DYRK1A/GSK3β inhibitor [3]. It was designed and reported by the Genomics Institute of the Novartis Research Foundation (GNF) as a strategy to increase β-cell mass and insulin levels, and to improve glycemic control as a novel therapy for type 1 diabetes. Although experiments conducted by the GNF team indicate that DYRK1A inhibition alone is sufficient to promote pancreatic β-cell proliferation, dual inhibition is synergistic. However, GSK3β inhibition may pose a cancer risk [1], so selective DYRK1A inhibitors have subsequently been designed (e.g. GNF2133) to ascertain the efficacy of DYRK1A inhibition alone [2].
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Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

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View interactive charts of activity data across species

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2D Structure
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2D Structure

An image of the ligand's 2D structure. For small molecules with SMILES these are drawn using the NCI/CADD Chemical Identifier Resolver. Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. For other types of ligands, e.g. longer nucleotides and peptides, a manually drawn representation of the molecule may be provided.

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Physico-chemical Properties
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Physico-chemical Properties

Calculated molecular properties are available for small molecules and natural products (not peptides). Properties were generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/). All properties were selected to enable the prediction of the Lipinski Rule-of-Five profile or ‘druglikeness’ for each ligand. For more info on each category see the help pages.

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Hydrogen bond acceptors 9
Hydrogen bond donors 3
Rotatable bonds 8
Topological polar surface area 143.56
Molecular weight 494.21
XLogP 2.9
No. Lipinski's rules broken 0

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
SMILES / InChI / InChIKey
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SMILES / InChI / InChIKey

SMILES (Simplified Molecular Input Line Entry Specification) A specification for unambiguously describing the structure of chemical molecules using short ASCII strings. Canonical SMILES specify a unique representation of the 2D structure without chiral or isotopic specifications. Isomeric SMILES include chiral specification and isotopes.

Standard InChI (IUPAC International Chemical Identifier) and InChIKey InChI is a non-proprietary, standard, textual identifier for chemical substances designed to facilitate linking of information and database searching. An InChIKey is a simplified version of a full InChI, designed for easier web searching.

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)

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Canonical SMILES CC(Oc1ccc(c(c1)c1cnc(c(n1)C(=O)Nc1cnccc1N1CCC[C@H](C1)C(=O)O)N)F)C
Isomeric SMILES CC(Oc1ccc(c(c1)c1cnc(c(n1)C(=O)Nc1cnccc1N1CCC[C@H](C1)C(=O)O)N)F)C
InChI InChI=1S/C25H27FN6O4/c1-14(2)36-16-5-6-18(26)17(10-16)19-12-29-23(27)22(30-19)24(33)31-20-11-28-8-7-21(20)32-9-3-4-15(13-32)25(34)35/h5-8,10-12,14-15H,3-4,9,13H2,1-2H3,(H2,27,29)(H,31,33)(H,34,35)/t15-/m1/s1
InChI Key UZIATSFXNVOVFE-OAHLLOKOSA-N

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
Bioactivity Comments
GNF4877 promotes proliferation of human pancreatic β-cells in vitro, and increases β-cell mass, insulin content, and improves glycaemic control in mice in vivo [3]. These findings support targeting β-cell proliferation as a potential novel strategy to combat type I diabetes. In a kinase selectivity screening panel 200 nM GNF4877 inhibits DYRK1A by 100% and GSK3β by 99%. At this concentration GNF4877 also inhibits SYK (89%), AXL (74%), ROS (71%) and CDK2 (67%).
Selectivity at enzymes
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
dual specificity tyrosine phosphorylation regulated kinase 1A Hs Inhibitor Inhibition 8.2 pIC50 - 3
⤷ pIC50 8.2 (IC50 6x10-9 M) [3]
glycogen synthase kinase 3 beta Hs Inhibitor Inhibition 7.8 pIC50 - 3
⤷ pIC50 7.8 (IC50 1.6x10-8 M) [3]