Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Plasmodium falciparum 3D7	-	746		PRS	proline--tRNA ligase

Previous and Unofficial Names

Plasmodium falciparum prolyl-tRNA synthetase | PfPRORS

Database Links
ChEMBL Target	CHEMBL3301560 (Pf3D7)
PlasmoDB	PF3D7_1213800 (Pf3D7)
SynPHARM	85927 (in complex with halofuginone)
UniProtKB	Q8I5R7 (Pf3D7)

Selected 3D Structures
	Description:  Crystal Structure of Prolyl-tRNA Synthetase (PRS) from Plasmodium falciparum in complex with Halofuginone and AMPPNP PDB Id:  4YDQ Ligand:  halofuginone Resolution:  2.3Å Species:  Plasmodium falciparum References:  3
	Description:  Crystal Structure of Prolyl-tRNA Synthetase (PRS) from Plasmodium falciparum PDB Id:  4TWA Resolution:  3.0Å Species:  Plasmodium falciparum References:  2

Whole Organism Assay Data Linked to This Target
Key to terms and symbols		Click column headers to sort
Ligand Sp. Assay description Type Action Value Parameter Reference halofuginone PfDd2 Plasmodium falciparum Dd2 P. falciparum strain Dd2 (PfDd2) is a clone derived from PfW2, following continuous culture in mefloquine. PfW2 was cloned from the Indochina III/CDC isolate, originally derived from a Laotian patient who failed chloroquine therapy. PfDd2 can be obtained from the Malaria Research and Reference Reagent Resource Center (MR4) and is reported to be resistant to chloroquine, pyrimethamine and mefloquine. ✖ Parasite growth inhibition assay - - 9.1 pEC50 1 ⤷ pEC50 9.1 (EC50 8.1x10-10 M) SYBR Green assay [1] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) halofuginone Pf3D7 Plasmodium falciparum 3D7 P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1). Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. ✖ Parasite growth inhibition assay - - 9.1 pEC50 1 ⤷ pEC50 9.1 (EC50 8.6x10-10 M) SYBR Green assay [1] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont)

Malaria Pharmacology Comments

The aminoacyl-tRNA synthetases are emerging targets for antimalarial agents [4]. Although sharing a common tRNA esterification catalytic activity, these proteins are structurally diverse and physiologically distinct enzymes.