Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	393	7q22.1	NAPEPLD	N-acyl phosphatidylethanolamine phospholipase D
Mouse	-	396	5 A3	Napepld	N-acyl phosphatidylethanolamine phospholipase D
Rat	-	396	4q11	Napepld	N-acyl phosphatidylethanolamine phospholipase D

Previous and Unofficial Names

chromosome 7 open reading frame 18, N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D | FMP30

Database Links
Alphafold	Q6IQ20 (Hs), Q8BH82 (Mm), Q769K2 (Rn)
BRENDA	3.1.4.54
CATH/Gene3D	3.60.15.10
ChEMBL Target	CHEMBL4630839 (Hs), CHEMBL2163172 (Mm)
Ensembl Gene	ENSG00000161048 (Hs), ENSMUSG00000044968 (Mm), ENSRNOG00000011363 (Rn)
Entrez Gene	222236 (Hs), 242864 (Mm), 296757 (Rn)
Human Protein Atlas	ENSG00000161048 (Hs)
KEGG Enzyme	3.1.4.54
KEGG Gene	hsa:222236 (Hs), mmu:242864 (Mm), rno:296757 (Rn)
OMIM	612334 (Hs)
Pharos	Q6IQ20 (Hs)
UniProtKB	Q6IQ20 (Hs), Q8BH82 (Mm), Q769K2 (Rn)
Wikipedia	NAPEPLD (Hs)

Selected 3D Structures
	Description:  Crystal structure of NAPE-PLD in complex with 1,3,7-Naphthalenetrisulfonic acid PDB Id:  8P96 Ligand:  N3SA Resolution:  2.86Å Species:  Human References:  3
	Description:  Crystal structure of NAPE-PLD in complex with hydrochlorothiazide PDB Id:  8PC4 Ligand:  hydrochlorothiazide Resolution:  2.85Å Species:  Human References:  3

Enzyme Reaction
EC Number: 3.1.4.54

Download all structure-activity data for this target as a CSV file

Inhibitors
Key to terms and symbols	View all chemical structures	Click column headers to sort
Ligand Sp. Action Value Parameter Reference LEI-401 Hs Inhibition 7.6 pKi 4 ⤷ pKi 7.6 (Ki 2.7x10-8 M) [4] Description: hNAPE-PLD in vitro activity assay. hexachlorophene Hs Inhibition 5.0 pIC50 1 ⤷ pIC50 5.0 (IC50 9.8x10-6 M) [1] bithionol Hs Inhibition 5.0 pIC50 1 ⤷ pIC50 5.0 (IC50 1.07x10-5 M) [1] ARN19874 Hs Inhibition 4.5 pIC50 2 ⤷ pIC50 4.5 (IC50 3.37x10-5 M) [2]

Allosteric Modulators
Key to terms and symbols	View all chemical structures	Click column headers to sort
Ligand Sp. Action Value Parameter Reference N3SA Hs Binding 7.5 pKd 3 ⤷ pKd 7.5 (Kd 3.3x10-8 M) [3] Description: Equilibrium dissociation constant determined in the presence of DCA indapamide Hs Binding 6.2 pKd 3 ⤷ pKd 6.2 (Kd 5.7x10-7 M) [3] Description: Equilibrium dissociation constant determined in the presence of DCA chlorthalidone Hs Binding 6.2 pKd 3 ⤷ pKd 6.2 (Kd 6.8x10-7 M) [3] Description: Equilibrium dissociation constant determined in the presence of DCA hydrochlorothiazide Hs Binding 5.1 – 6.1 pKd 3 ⤷ pKd 6.1 (Kd 7.4x10-7 M) [3] Description: equilibrium dissociation constant in presence of deoxycholic acid, determined by SPR pKd 5.1 (Kd 8x10-6 M) [3] Description: Determined by surface plasmon resonance, in the absence of deoxycholic acid in the bile acid binding domain
Allosteric Modulator Comments
Thiazide and thiazide-like drugs mediate allosteric stabilization of NAPE-PLD dimers [3]. This mechanism contributes directly to thiazide-induced reduction in vascular resistance, in additional to their established diuretic effect via inhibition of the Na-Cl symporter (SLC12A3).

Immuno Process Associations

Immuno Process:  Inflammation

Immuno Process:  Immune regulation