Neuronal NOS | Nitric oxide synthases | IUPHAR/MMV Guide to MALARIA PHARMACOLOGY

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Neuronal NOS

Target id: 1251

Nomenclature: Neuronal NOS

Abbreviated Name: nNOS

Family: Nitric oxide synthases

Gene and Protein Information
Species TM AA Chromosomal Location Gene Symbol Gene Name Reference
Human - - NOS1 nitric oxide synthase 1
Mouse - - Nos1 nitric oxide synthase 1
Rat - - Nos1 nitric oxide synthase 1
Previous and Unofficial Names
brain NOS | NOS1 | constitutive NOS | NC-NOS | neuronal NOS | nitric oxide synthase 1 | Nitric oxide synthase 1 (neuronal) | Nitric oxide synthase 1 / NOSI | nitric oxide synthase 1 (neuronal)
Database Links
ChEMBL Target
Ensembl Gene
Entrez Gene
Human Protein Atlas
KEGG Enzyme
Selected 3D Structures
Image of receptor 3D structure from RCSB PDB
Description:  Structure of rat neuronal nitric oxide synthase heme domain in complex with 4-methyl-6-(2-(5-(3-(methylamino)propyl)pyridin-3-yl) ethyl)pyridin-2-amine
Ligand:  compound 14j [PMID: 27050842]
Resolution:  1.95Å
Species:  Rat
References:  3
Enzyme Reaction
EC Number:
Product Species Reference
NO Human
L-citrulline Human
Cofactor Species Comments Reference
Zn2+ Human
heme Human
flavin adenine dinucleotide Human
flavin mononucleotide Human
BH4 Human
oxygen Human
Endogenous Substrate (Human)

Download all structure-activity data for this target as a CSV file

Key to terms and symbols View all chemical structures Click column headers to sort
Ligand Sp. Action Value Parameter Reference
compound 14j [PMID: 27050842] Hs Inhibition 7.9 pKi 3
pKi 7.9 (Ki 1.3x10-8 M) [3]
L-NNA Hs Inhibition 7.8 pKi 1
pKi 7.8 (Ki 1.5x10-8 M) [1]
compound 14j [PMID: 27050842] Rn Inhibition 7.8 pKi 3
pKi 7.8 (Ki 1.6x10-8 M) [3]
Nωpropyl-L-arginine Rn Inhibition 7.2 pKi 4
pKi 7.2 [4]
GW274150 Hs Inhibition 4.7 pIC50 2
pIC50 4.7 (IC50 1.8x10-5 M) [2]
NANT N/A Inhibition - -
View species-specific inhibitor tables


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1. Collins JL, Shearer BG, Oplinger JA, Lee S, Garvey EP, Salter M, Duffy C, Burnette TC, Furfine ES. (1998) N-Phenylamidines as selective inhibitors of human neuronal nitric oxide synthase: structure-activity studies and demonstration of in vivo activity. J. Med. Chem., 41 (15): 2858-71. [PMID:9667974]

2. Connolly S, Aberg A, Arvai A, Beaton HG, Cheshire DR, Cook AR, Cooper S, Cox D, Hamley P, Mallinder P et al.. (2004) 2-aminopyridines as highly selective inducible nitric oxide synthase inhibitors. Differential binding modes dependent on nitrogen substitution. J. Med. Chem., 47 (12): 3320-3. [PMID:15163211]

3. Wang HY, Qin Y, Li H, Roman LJ, Martásek P, Poulos TL, Silverman RB. (2016) Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibition by Optimization of the 2-Aminopyridine-Based Scaffold with a Pyridine Linker. J. Med. Chem., 59 (10): 4913-25. [PMID:27050842]

4. Zhang HQ, Fast W, Marletta MA, Martasek P, Silverman RB. (1997) Potent and selective inhibition of neuronal nitric oxide synthase by N omega-propyl-L-arginine. J. Med. Chem., 40 (24): 3869-70. [PMID:9397167]


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