Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	1153	17q11.2	NOS2	nitric oxide synthase 2
Mouse	-	1144	11 46.74 cM	Nos2	nitric oxide synthase 2, inducible
Rat	-	1147	10q25	Nos2	nitric oxide synthase 2

Previous and Unofficial Names

NOS2A | HEP-NOS | nitric oxide synthase 2A (inducible, hepatocytes) | Nitric oxide synthase 2 inducible | Nitric oxide synthase 2 inducible macrophage | inducible NO synthase | Nitric oxide synthase 2 / NOSII | inducible macrophage NOS | nitric oxide synthase 2

Database Links
Alphafold	P35228 (Hs), P29477 (Mm), Q06518 (Rn)
BRENDA	1.14.13.39
CATH/Gene3D	3.40.50.360
ChEMBL Target	CHEMBL4481 (Hs), CHEMBL3464 (Mm), CHEMBL3051 (Rn)
DrugBank Target	P35228 (Hs)
Ensembl Gene	ENSG00000007171 (Hs), ENSMUSG00000020826 (Mm), ENSRNOG00000057443 (Rn)
Entrez Gene	4843 (Hs), 18126 (Mm), 24599 (Rn)
Human Protein Atlas	ENSG00000007171 (Hs)
KEGG Enzyme	1.14.13.39
KEGG Gene	hsa:4843 (Hs), mmu:18126 (Mm), rno:24599 (Rn)
OMIM	163730 (Hs)
Pharos	P35228 (Hs)
UniProtKB	P35228 (Hs), P29477 (Mm), Q06518 (Rn)
Wikipedia	NOS2 (Hs)

Enzyme Reaction
EC Number: 1.14.13.39

Products
Product Species Reference NO Human L-citrulline Human

Cofactors
Cofactor Species Comments Reference Zn2+ Human NADPH Human heme Human flavin adenine dinucleotide Human flavin mononucleotide Human BH4 Human oxygen Human

Endogenous Substrate (Human)

L-arginine

Download all structure-activity data for this target as a CSV file

Inhibitors
Key to terms and symbols	View all chemical structures	Click column headers to sort
Ligand Sp. Action Value Parameter Reference L-NNA Hs Inhibition 6.2 pKi 2 ⤷ pKi 6.2 (Ki 6.7x10-7 M) [2] tilarginine Hs Inhibition 6.1 pKi 4 ⤷ pKi 6.1 (Ki 8.6x10-7 M) [4] GW274150 Hs Inhibition 7.2 pIC50 3 ⤷ pIC50 7.2 (IC50 7x10-8 M) [3]

Immunopharmacology Comments

The role of iNOS in immuno-oncology is reviewed in [1].

Immuno Process Associations

Immuno Process:  Antigen presentation GO Annotations:  Associated to 1 GO processes GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis IMP

Immuno Process:  Cellular signalling GO Annotations:  Associated to 1 GO processes GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis IMP

Immuno Process:  Cytokine production & signalling GO Annotations:  Associated to 4 GO processes GO:0032755 positive regulation of interleukin-6 production IDA GO:0032757 positive regulation of interleukin-8 production IDA GO:1900015 regulation of cytokine production involved in inflammatory response IDA click arrow to show/hide IEA associations GO:0071346 cellular response to type II interferon IEA

Immuno Process:  Immune regulation GO Annotations:  Associated to 3 GO processes GO:0001912 positive regulation of leukocyte mediated cytotoxicity TAS GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis IMP GO:1900015 regulation of cytokine production involved in inflammatory response IDA

Immuno Process:  Inflammation GO Annotations:  Associated to 5 GO processes GO:0002227 innate immune response in mucosa NAS GO:0006954 inflammatory response IBA GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis IMP GO:1900015 regulation of cytokine production involved in inflammatory response IDA click arrow to show/hide IEA associations GO:0071346 cellular response to type II interferon IEA