compound 101 [PMID: 21596927]   Click here for help

GtoPdb Ligand ID: 8437

Synonyms: cmpd101 | Takeda compound 101
PDB Ligand
Compound class: Synthetic organic
Comment: Compound 101 is an inhibitor of the beta adrenergic receptor kinase 1 (aka GRK2, gene symbol ADRBK1) and beta adrenergic receptor kinase 2 (aka GRK3, gene symbol ADRBK2) [3]. Compound 101 is one of the chemical structures covered by patent WO2007034846 A1 [1].
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Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

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View interactive charts of activity data across species

compound 101 [PMID: 21596927] is commercially available from our sponsors

2D Structure
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2D Structure

An image of the ligand's 2D structure. For small molecules with SMILES these are drawn using the NCI/CADD Chemical Identifier Resolver. Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. For other types of ligands, e.g. longer nucleotides and peptides, a manually drawn representation of the molecule may be provided.

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Physico-chemical Properties
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Physico-chemical Properties

Calculated molecular properties are available for small molecules and natural products (not peptides). Properties were generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/). All properties were selected to enable the prediction of the Lipinski Rule-of-Five profile or ‘druglikeness’ for each ligand. For more info on each category see the help pages.

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Hydrogen bond acceptors 6
Hydrogen bond donors 2
Rotatable bonds 9
Topological polar surface area 84.73
Molecular weight 466.17
XLogP 5.46
No. Lipinski's rules broken 1

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
SMILES / InChI / InChIKey
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SMILES / InChI / InChIKey

SMILES (Simplified Molecular Input Line Entry Specification) A specification for unambiguously describing the structure of chemical molecules using short ASCII strings. Canonical SMILES specify a unique representation of the 2D structure without chiral or isotopic specifications. Isomeric SMILES include chiral specification and isotopes.

Standard InChI (IUPAC International Chemical Identifier) and InChIKey InChI is a non-proprietary, standard, textual identifier for chemical substances designed to facilitate linking of information and database searching. An InChIKey is a simplified version of a full InChI, designed for easier web searching.

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)

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Canonical SMILES O=C(c1cccc(c1)NCc1nnc(n1C)c1ccncc1)NCc1ccccc1C(F)(F)F
Isomeric SMILES O=C(c1cccc(c1)NCc1nnc(n1C)c1ccncc1)NCc1ccccc1C(F)(F)F
InChI InChI=1S/C24H21F3N6O/c1-33-21(31-32-22(33)16-9-11-28-12-10-16)15-29-19-7-4-6-17(13-19)23(34)30-14-18-5-2-3-8-20(18)24(25,26)27/h2-13,29H,14-15H2,1H3,(H,30,34)
InChI Key WFOVEDJTASPCIR-UHFFFAOYSA-N

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
Bioactivity Comments
Compound 101 is selective for the GRK2 subfamily (GRK2 and GRK3) of G protein-coupled receptor kinases [3]. Subsequent profile screening (of 58 kinases representative of the entire kinome, excl. lipid kinases) identifies only 5 off-target kinases inhibited by compound 101 by >50% (@ 1μM): protein kinase C related protein kinase (PRK2), serum and glucocorticoid regulated kinase (SGK1), ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RSK1) , ribosomal protein S6 kinase, 90kDa, polypeptide 5 (MSK1) and ribosomal protein S6 kinase, 70kDa, polypeptide 1 (S6K1) [2].
A role for these GPCR-related kinases in desensitisation of activated μ opioid receptors is identified by experiments showing that compound 101-induced inhibition of GRK2/3 partially reverses agonist-induced desensitisation of the μ opioid receptor [2].
Selectivity at enzymes
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
beta adrenergic receptor kinase 2 Primary target of this compound Hs Inhibitor Inhibition 7.5 pIC50 - 3
⤷ pIC50 7.5 (IC50 3.2x10-8 M) [3]
beta adrenergic receptor kinase 1 Primary target of this compound Hs Inhibitor Inhibition 7.3 pIC50 - 3
⤷ pIC50 7.3 (IC50 5.4x10-8 M) [3]