balanol   Click here for help

GtoPdb Ligand ID: 8142

Synonyms: azepinostatin
PDB Ligand
Compound class: Natural product
Comment: Balanol is a fungal metabolite which acts as a non-selective ATP-competitive inhibitor of protein kinases A, G and C (AGC family kinases) [1-3]. Balanol is also reported to inhibit G protein-coupled receptor kinases (GRKs), with modest selectivity for the GRK2 family members, GRK2 and GRK3 [4].
Click here for help

Ligand Activity Visualisation Charts

These are box plot that provide a unique visualisation, summarising all the activity data for a ligand taken from ChEMBL and GtoPdb across multiple targets and species. Click on a plot to see the median, interquartile range, low and high data points. A value of zero indicates that no data are available. A separate chart is created for each target, and where possible the algorithm tries to merge ChEMBL and GtoPdb targets by matching them on name and UniProt accession, for each available species. However, please note that inconsistency in naming of targets may lead to data for the same target being reported across multiple charts.

✖

View interactive charts of activity data across species
2D Structure
Click here for help

2D Structure

An image of the ligand's 2D structure. For small molecules with SMILES these are drawn using the NCI/CADD Chemical Identifier Resolver. Click on the image to access the chemical structure search tool with the ligand pre-loaded in the structure editor. For other types of ligands, e.g. longer nucleotides and peptides, a manually drawn representation of the molecule may be provided.

✖
Click here for structure editor
Physico-chemical Properties
Click here for help

Physico-chemical Properties

Calculated molecular properties are available for small molecules and natural products (not peptides). Properties were generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/). All properties were selected to enable the prediction of the Lipinski Rule-of-Five profile or ‘druglikeness’ for each ligand. For more info on each category see the help pages.

✖
Hydrogen bond acceptors 8
Hydrogen bond donors 7
Rotatable bonds 9
Topological polar surface area 202.72
Molecular weight 550.16
XLogP 4.2
No. Lipinski's rules broken 1

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
SMILES / InChI / InChIKey
Click here for help

SMILES / InChI / InChIKey

SMILES (Simplified Molecular Input Line Entry Specification) A specification for unambiguously describing the structure of chemical molecules using short ASCII strings. Canonical SMILES specify a unique representation of the 2D structure without chiral or isotopic specifications. Isomeric SMILES include chiral specification and isotopes.

Standard InChI (IUPAC International Chemical Identifier) and InChIKey InChI is a non-proprietary, standard, textual identifier for chemical substances designed to facilitate linking of information and database searching. An InChIKey is a simplified version of a full InChI, designed for easier web searching.

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)

✖
Canonical SMILES Oc1ccc(cc1)C(=O)NC1CNCCCC1OC(=O)c1cc(O)c(c(c1)O)C(=O)c1c(O)cccc1C(=O)O
Isomeric SMILES Oc1ccc(cc1)C(=O)N[C@@H]1CNCCC[C@H]1OC(=O)c1cc(O)c(c(c1)O)C(=O)c1c(O)cccc1C(=O)O
InChI InChI=1S/C28H26N2O10/c31-16-8-6-14(7-9-16)26(36)30-18-13-29-10-2-5-22(18)40-28(39)15-11-20(33)24(21(34)12-15)25(35)23-17(27(37)38)3-1-4-19(23)32/h1,3-4,6-9,11-12,18,22,29,31-34H,2,5,10,13H2,(H,30,36)(H,37,38)/t18-,22-/m1/s1
InChI Key XYUFCXJZFZPEJD-XMSQKQJNSA-N

Generated using the Chemistry Development Kit (CDK) (Willighagen EL et al. Journal of Cheminformatics vol. 9:33. 2017, doi:10.1186/s13321-017-0220-4; https://cdk.github.io/)
Bioactivity Comments
IC50 values for banalol inhibition of GRK1 and GRK3 subfamily members are reported by Tesmer et al (2010) [4], in Table 1. We have mapped PKCη as the primary target of balanol, based only on its virtue of having the lowest IC50 value. The interaction table below makes clear the broad spectrum activity of this compound.
Selectivity at enzymes
Key to terms and symbols Click column headers to sort
Target Sp. Type Action Value Parameter Concentration range (M) Reference
protein kinase A Rn Inhibitor Inhibition 10.4 pIC50 - 1
⤷ pIC50 10.4 (IC50 4x10-11 M) [1]
Description: Inhibition of PKA extracted from rat brain homogenate.
protein kinase C eta Primary target of this compound Hs Inhibitor Inhibition 8.5 pIC50 - 1
⤷ pIC50 8.5 (IC50 3x10-9 M) [1]
protein kinase C beta Hs Inhibitor Inhibition 8.0 pIC50 - 1
⤷ pIC50 8.0 (IC50 1x10-8 M) [1]
protein kinase C gamma Hs Inhibitor Inhibition 8.0 pIC50 - 1
⤷ pIC50 8.0 (IC50 1x10-8 M) [1]
protein kinase C delta Hs Inhibitor Inhibition 7.8 pIC50 - 1
⤷ pIC50 7.8 (IC50 1.6x10-8 M) [1]
protein kinase C epsilon Hs Inhibitor Inhibition 7.7 pIC50 - 1
⤷ pIC50 7.7 (IC50 2x10-8 M) [1]
protein kinase C alpha Hs Inhibitor Inhibition 7.5 pIC50 - 1
⤷ pIC50 7.5 (IC50 3x10-8 M) [1]
beta adrenergic receptor kinase 1 Hs Inhibitor Inhibition 7.4 pIC50 - 4
⤷ pIC50 7.4 (IC50 4.2x10-8 M) [4]
Description: Inhibition of tubulin phosphorylation by balanol.
beta adrenergic receptor kinase 2 Hs Inhibitor Inhibition 7.3 pIC50 - 4
⤷ pIC50 7.3 (IC50 4.7x10-8 M) [4]
G protein-coupled receptor kinase 7 Hs Inhibitor Inhibition 6.7 pIC50 - 4
⤷ pIC50 6.7 (IC50 1.8x10-7 M) [4]
G protein-coupled receptor kinase 4 Hs Inhibitor Inhibition 6.6 pIC50 - 4
⤷ pIC50 6.6 (IC50 2.6x10-7 M) [4]
Description: Measuring inhibition of human GRK-4-mediated phosphorylation of bovine tubulin dimers.
G protein-coupled receptor kinase 1 Hs Inhibitor Inhibition 6.5 pIC50 - 4
⤷ pIC50 6.5 (IC50 3.4x10-7 M) [4]
G protein-coupled receptor kinase 6 Hs Inhibitor Inhibition 6.3 pIC50 - 4
⤷ pIC50 6.3 (IC50 4.9x10-7 M) [4]
protein kinase C zeta Hs Inhibitor Inhibition 5.2 pIC50 - 1
⤷ pIC50 5.2 (IC50 5.9x10-6 M) [1]