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ChEMBL ligand: CHEMBL60254 (Balanol) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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Beta-adrenergic receptor kinase 1 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3711550] [UniProtKB: P21146] | ||||||||
ChEMBL | Inhibition of bovine GRK2 S670A mutant expressed in Baculovirus expression system assessed as decrease in phosphorylation of urea-washed bovine rod outer segments preincubated for 30 mins followed by ATP addition and light exposure measured after 5 to 10 mins by SDS-PAGE analysis | B | 7.46 | pIC50 | 35 | nM | IC50 | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
beta adrenergic receptor kinase 2/Beta-adrenergic receptor kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075166] [GtoPdb: 1467] [UniProtKB: P35626] | ||||||||
ChEMBL | Inhibition of GRK3-mediated bovine tubulin phosphorylation by scintillation counting | B | 7.33 | pIC50 | 47 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
GtoPdb | - | - | 7.33 | pIC50 | 47 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
protein kinase, cAMP-dependent, catalytic, alpha subunit/cAMP-dependent protein kinase alpha-catalytic subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4101] [GtoPdb: 1476] [UniProtKB: P17612] | ||||||||
ChEMBL | Binding affinity to PKAalpha (unknown origin) using Lys-Arg-Thr-Leu-Arg-Arg as substrate after 8 mins in presence of [gamma-32P]ATP by liquid scintillation spectrometry | B | 8.41 | pKd | 3.9 | nM | Kd | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
ChEMBL | Inhibitory concentration against recombinant human cAMP-dependent Protein kinase A | B | 7.3 | pIC50 | 50 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Inhibition of human PKAC-alpha | B | 8.41 | pIC50 | 3.9 | nM | IC50 | Eur J Med Chem (2022) 243: 114668-114668 [PMID:36055000] |
Protein kinase G (PKG) 1/cGMP-dependent protein kinase 1 beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4273] [GtoPdb: 1492] [UniProtKB: Q13976] | ||||||||
ChEMBL | Binding affinity to human recombinant N-terminal GST-tagged PKGalpha expressed in Baculovirus infected insect Sf9 cells using RKRSRAE as substrate in presence of [gamma-32P]ATP | B | 8.8 | pKd | 1.6 | nM | Kd | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
beta adrenergic receptor kinase 1/G-protein coupled receptor kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4079] [GtoPdb: 1466] [UniProtKB: P25098] | ||||||||
ChEMBL | Inhibition of GRK2-mediated bovine tubulin phosphorylation by scintillation counting | B | 7.38 | pIC50 | 42 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
ChEMBL | Inhibition of GRK2 (unknown origin) | B | 7.38 | pIC50 | 42 | nM | IC50 | Eur J Med Chem (2022) 243: 114668-114668 [PMID:36055000] |
GtoPdb | Inhibition of tubulin phosphorylation by balanol. | - | 7.38 | pIC50 | 42 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
G protein-coupled receptor kinase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5861] [GtoPdb: 1468] [UniProtKB: P32298] | ||||||||
ChEMBL | Inhibition of GRK4-mediated bovine tubulin phosphorylation by scintillation counting | B | 6.59 | pIC50 | 260 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
GtoPdb | Measuring inhibition of human GRK-4-mediated phosphorylation of bovine tubulin dimers. | - | 6.59 | pIC50 | 260 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
G protein-coupled receptor kinase 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5678] [GtoPdb: 1469] [UniProtKB: P34947] | ||||||||
ChEMBL | Inhibition of GRK5-mediated bovine tubulin phosphorylation by scintillation counting | B | 6.8 | pIC50 | 160 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
G protein-coupled receptor kinase 5 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3879830] [UniProtKB: P43249] | ||||||||
ChEMBL | Inhibition of bovine C-terminal truncated GRK5 (561 residues) assessed as decrease in phosphorylation of urea-washed bovine rod outer segments preincubated for 30 mins followed by ATP addition and light exposure measured after 5 to 10 mins by SDS-PAGE analysis | B | 6.36 | pIC50 | 440 | nM | IC50 | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
G protein-coupled receptor kinase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6144] [GtoPdb: 1470] [UniProtKB: P43250] | ||||||||
ChEMBL | Inhibition of GRK6-mediated bovine tubulin phosphorylation by scintillation counting | B | 6.31 | pIC50 | 490 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
GtoPdb | - | - | 6.31 | pIC50 | 490 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
G protein-coupled receptor kinase 7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075133] [GtoPdb: 1471] [UniProtKB: Q8WTQ7] | ||||||||
GtoPdb | - | - | 6.74 | pIC50 | 180 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
ChEMBL | Inhibition of GRK7-mediated bovine tubulin phosphorylation by scintillation counting | B | 6.74 | pIC50 | 180 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
protein kinase C alpha/Protein kinase C alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL299] [GtoPdb: 1482] [UniProtKB: P17252] | ||||||||
ChEMBL | Binding affinity to PKCalpha (unknown origin) using Lys-Arg-Thr-Leu-Arg-Arg as substrate after 8 mins in presence of [gamma-32P]ATP by liquid scintillation spectrometry | B | 8.19 | pKd | 6.4 | nM | Kd | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
ChEMBL | Inhibition of Protein kinase C alpha expressed in Sf-9 cells | B | 7.13 | pIC50 | 74 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2133-2138 |
ChEMBL | Inhibition of human Protein kinase C alpha | B | 7.13 | pIC50 | 74 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of human Protein kinase C alpha | B | 7.15 | pIC50 | 70 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibition of Protein kinase C alpha | B | 7.17 | pIC50 | 67 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
GtoPdb | - | - | 7.52 | pIC50 | 30 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C alpha isozyme | B | 7.52 | pIC50 | 30 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Evaluated against recombinant human Protein kinase C alpha | B | 7.52 | pIC50 | 30 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
ChEMBL | Inhibition of Protein kinase C alpha | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
protein kinase C beta/Protein kinase C beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3045] [GtoPdb: 1483] [UniProtKB: P05771] | ||||||||
ChEMBL | Binding affinity to PKCbeta2 (unknown origin) using Lys-Arg-Thr-Leu-Arg-Arg as substrate after 8 mins in presence of [gamma-32P]ATP by liquid scintillation spectrometry | B | 8.74 | pKd | 1.8 | nM | Kd | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
ChEMBL | Inhibition of Protein kinase C beta 2 expressed in Sf-9 cells | B | 7.36 | pIC50 | 44 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2133-2138 |
ChEMBL | Inhibition of human Protein kinase C beta 2 | B | 7.36 | pIC50 | 44 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of human Protein kinase C beta 2 | B | 7.4 | pIC50 | 40 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibition of human Protein kinase C beta 1 | B | 7.49 | pIC50 | 32 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of Protein kinase C beta 2 | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of Protein kinase C beta 1 | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of human Protein kinase C beta 1 | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
GtoPdb | - | - | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Evaluated against recombinant human Protein kinase C beta 1 | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
ChEMBL | Evaluated against recombinant human Protein kinase C beta 2 | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C beta 2 isozyme | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Inhibition of Protein kinase C beta 1 | B | 8 | pIC50 | 10 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C beta 1 isozyme | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Inhibition of Protein kinase C beta 2 | B | 8 | pIC50 | 10 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
protein kinase C delta/Protein kinase C delta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2996] [GtoPdb: 1485] [UniProtKB: Q05655] | ||||||||
ChEMBL | Inhibition of Protein kinase C delta expressed in Sf-9 cells | B | 7.49 | pIC50 | 32 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2133-2138 |
ChEMBL | Inhibition of human Protein kinase C delta | B | 7.49 | pIC50 | 32 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of human Protein kinase C delta | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibition of Protein kinase C delta | B | 7.64 | pIC50 | 23 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Evaluated against recombinant human Protein kinase C delta | B | 7.7 | pIC50 | 20 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
ChEMBL | Inhibition of Protein kinase C delta | B | 7.8 | pIC50 | 16 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
GtoPdb | - | - | 7.8 | pIC50 | 16 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C delta isozyme | B | 8.4 | pIC50 | 4 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
protein kinase C epsilon/Protein kinase C epsilon in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3582] [GtoPdb: 1486] [UniProtKB: Q02156] | ||||||||
ChEMBL | Inhibition of Protein kinase C epsilon | B | 7.3 | pIC50 | 50 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibition of protein kinase C epsilon expressed in Sf-9 cells | B | 7.31 | pIC50 | 49 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2133-2138 |
ChEMBL | Inhibition of human Protein kinase C epsilon | B | 7.31 | pIC50 | 49 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of Protein kinase C epsilon | B | 7.42 | pIC50 | 38 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of Protein kinase C epsilon | B | 7.7 | pIC50 | 20 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Evaluated against recombinant human Protein kinase C epsilon | B | 7.7 | pIC50 | 20 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
GtoPdb | - | - | 7.7 | pIC50 | 20 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C epsilon isozyme | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
protein kinase C eta/Protein kinase C eta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3616] [GtoPdb: 1487] [UniProtKB: P24723] | ||||||||
ChEMBL | Inhibition of human protein kinase C eta | B | 7.66 | pIC50 | 22 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of Protein kinase C eta expressed in Sf-9 cells | B | 7.66 | pIC50 | 22 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2133-2138 |
ChEMBL | Inhibition of Protein Kinase C eta | B | 7.7 | pIC50 | 20 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibition of Protein kinase C eta | B | 7.7 | pIC50 | 20 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of PKCeta (unknown origin) by IMAP kinase assay | B | 8.4 | pIC50 | 4 | nM | IC50 | J Med Chem (2022) 65: 3134-3150 [PMID:35167283] |
GtoPdb | - | - | 8.52 | pIC50 | 3 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Evaluated against recombinant human Protein kinase C eta | B | 8.52 | pIC50 | 3 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C eta isozyme | B | 8.52 | pIC50 | 3 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Inhibition of Protein kinase C eta | B | 8.52 | pIC50 | 3 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
protein kinase C gamma/Protein kinase C gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2938] [GtoPdb: 1484] [UniProtKB: P05129] | ||||||||
ChEMBL | Inhibition of human Protein kinase C gamma | B | 7.47 | pIC50 | 34 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of Protein kinase C gamma | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of Protein Kinase C gamma | B | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C gamma isozyme | B | 7.7 | pIC50 | 20 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
GtoPdb | - | - | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Inhibition of Protein kinase C gamma | B | 8 | pIC50 | 10 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Evaluated against recombinant human Protein kinase C gamma | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
protein kinase C zeta/Protein kinase C zeta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3438] [GtoPdb: 1491] [UniProtKB: Q05513] | ||||||||
ChEMBL | Inhibitory concentration against recombinant human Protein kinase C zeta isozyme | B | 5.13 | pIC50 | 7400 | nM | IC50 | J Med Chem (1997) 40: 226-235 [PMID:9003521] |
ChEMBL | Inhibition of Protein kinase C zeta | B | 5.23 | pIC50 | 5900 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
GtoPdb | - | - | 5.23 | pIC50 | 5900 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL | Inhibition of Protein kinase C zeta | B | 5.46 | pIC50 | 3500 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2151-2154 |
ChEMBL | Inhibition of Protein kinase C zeta | B | 5.46 | pIC50 | 3500 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2015-2020 |
ChEMBL | Inhibition of Protein kinase C zeta | B | 5.46 | pIC50 | 3500 | nM | IC50 | Bioorg Med Chem Lett (1995) 5: 2155-2160 |
G protein-coupled receptor kinase 1/Rhodopsin kinase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5607] [GtoPdb: 1465] [UniProtKB: Q15835] | ||||||||
ChEMBL | Inhibition of GRK1-mediated bovine tubulin phosphorylation by scintillation counting | B | 6.47 | pIC50 | 340 | nM | IC50 | J Med Chem (2010) 53: 1867-1870 [PMID:20128603] |
GtoPdb | - | - | 6.47 | pIC50 | 340 | nM | IC50 | J Med Chem (2010) 53: 1867-70 [PMID:20128603] |
Rhodopsin kinase in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3879860] [UniProtKB: P28327] | ||||||||
ChEMBL | Inhibition of bovine C-terminal truncated GRK1 (535 residues) assessed as decrease in phosphorylation of urea-washed bovine rod outer segments preincubated for 30 mins followed by ATP addition and light exposure measured after 5 to 10 mins by SDS-PAGE analysis | B | 5.39 | pIC50 | 4100 | nM | IC50 | J Med Chem (2016) 59: 9277-9294 [PMID:27362616] |
SRC proto-oncogene, non-receptor tyrosine kinase/Tyrosine-protein kinase SRC in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL267] [GtoPdb: 2206] [UniProtKB: P12931] | ||||||||
ChEMBL | Inhibition of src Protein Tyrosine Kinase | B | 5 | pIC50 | >10000 | nM | IC50 | J Med Chem (1996) 39: 5215-5227 [PMID:8978850] |
protein kinase A in Rat [GtoPdb: 1694] | ||||||||
GtoPdb | Inhibition of PKA extracted from rat brain homogenate. | - | 10.4 | pIC50 | 0.04 | nM | IC50 | J Med Chem (1996) 39: 5215-27 [PMID:8978850] |
ChEMBL data shown on this page come from version 34:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]