furosemide [Ligand Id: 4839] activity data from GtoPdb and ChEMBL
Click here for a description of the charts and data table
Please tell us if you are using this feature and what you think!
| ChEMBL ligand: CHEMBL35 (Frusetic, Oedemex, Rusyde, Furosemida, Furosemide, Aquamed, LB-502, Marsemide, Diuresal, Logirene, NSC-269420, Frumax, Lasix ret, Furoscix, Frusemide, Hydroled, Aluzine 500, Mirfat, Tenkofruse, Furosemidum, Frusid, Froop, Frumil, Dryptal, Aluzine 20, Lasix, Frusol, Aluzine 40) |
|---|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
There should be some charts here, you may need to enable JavaScript!
|
|
There should be some charts here, you may need to enable JavaScript!
|
|
There should be some charts here, you may need to enable JavaScript!
|
| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4235] [GtoPdb: 2763] [UniProtKB: P28845] | ||||||||
| ChEMBL | Inhibition of human 11beta-HSD1 expressed in HEK293 cells assessed as conversion of [3H]cortisone to [3H]cortisol by scintillation proximity assay in presence of NADPH | B | 5.42 | pIC50 | 3760 | nM | IC50 | Eur J Med Chem (2009) 44: 1167-1171 [PMID:18653260] |
| hydroxysteroid 11-beta dehydrogenase 1/11-beta-hydroxysteroid dehydrogenase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3910] [GtoPdb: 2763] [UniProtKB: P50172] | ||||||||
| ChEMBL | Inhibition of mouse 11beta-HSD1 expressed in HEK293 cells assessed as conversion of [3H]cortisone to [3H]cortisol by scintillation proximity assay in presence of NADPH | B | 5.42 | pIC50 | 3760 | nM | IC50 | Eur J Med Chem (2009) 44: 1167-1171 [PMID:18653260] |
| Adenosine receptor A1 in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2304404] [UniProtKB: P47745] | ||||||||
| ChEMBL | Binding affinity against adenosine A1 receptor in guinea pig forebrain membranes using N6-[3H]cyclohexyladenosine as radioligand | B | 4 | pKi | >100000 | nM | Ki | J Med Chem (1992) 35: 3066-3075 [PMID:1501234] |
| AQP4/Aquaporin-4 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5291512] [GtoPdb: 691] [UniProtKB: P47863] | ||||||||
| ChEMBL | Inhibition of rat AQP4 water channel expressed in Xenopus laevis oocytes assessed as inhibition of AQP1-mediated osmotic swelling incubated for 1 to 2 hrs by videomicroscopy analysis | B | 5 | pIC50 | 10000 | nM | IC50 | Eur J Med Chem (2020) 208: 112855-112855 [PMID:33007663] |
| ABCG2/Broad substrate specificity ATP-binding cassette transporter ABCG2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0] | ||||||||
| ChEMBL | Inhibition of ABCG2 (unknown origin) expressed in human HEK293-A cells membrane vesicles assessed inhibition of ABCG2-mediated urate transport activity by rapid filtration technique | B | 5.49 | pIC50 | 3200 | nM | IC50 | Eur J Med Chem (2022) 237: 114346-114346 [PMID:35483322] |
| carbonic anhydrase 1/Carbonic anhydrase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA1 by stopped-flow CO2 hydration method | B | 7.21 | pKi | 62 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| ChEMBL | Known Inhibitors of Carbonic Anhydrase: Compounds which are CAIs are well known in the art, see for example, Pastorekova et al, Journal of Enzyme Inhibition and Medicinal Chemistry, 19(3), 199-229, 2004. Methods for determining the carbonic anhydrase inhibitory activity of a compound, i.e. whether a compound can be classified as a CAI, are known in the art and may usefully identify further compounds which may be suitable for use in the present invention. Exemplary methods and levels of activity required for consideration as CAIs are described in US 20020022245 and the references cited therein, the contents of which are incorporated herein by reference, in particular Supuran et al, European Journal of Medicinal Chemistry, 1998, 33, 577-594 and Scozzafava et al, Journal of Medicinal Chemistry, 1999, 42 25, 3690-3700). | B | 7.1 | pIC50 | 80 | nM | IC50 | US-10172837-B2. Insulin sensitisers and methods of treatment (2019) |
| carbonic anhydrase 12/Carbonic anhydrase 12 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570] | ||||||||
| ChEMBL | Inhibition of human recombinant CA12 catalytic domain by stopped-flow CO2 hydration method | B | 6.58 | pKi | 261 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 13/Carbonic anhydrase 13 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2186] [GtoPdb: 2748] [UniProtKB: Q9D6N1] | ||||||||
| ChEMBL | Inhibition of mouse recombinant full length CA13 by stopped-flow CO2 hydration method | B | 6.26 | pKi | 550 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 14/Carbonic anhydrase 14 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA14 by stopped-flow CO2 hydration method | B | 7.28 | pKi | 52 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 2/Carbonic anhydrase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] | ||||||||
| ChEMBL | Displacement of dansylamide from human recombinant CA II expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by fluorescence microplate reader | B | 5.5 | pKd | 3140 | nM | Kd | J Med Chem (2024) 67: 15862-15872 [PMID:39161321] |
| ChEMBL | Binding affinity to human recombinant CA II assessed as dissociation constant | B | 6.2 | pKd | 630 | nM | Kd | J Med Chem (2024) 67: 15862-15872 [PMID:39161321] |
| ChEMBL | Inhibition of human recombinant full length CA2 by stopped-flow CO2 hydration method | B | 7.19 | pKi | 65 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 4/Carbonic anhydrase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA4 by stopped-flow CO2 hydration method | B | 6.25 | pKi | 564 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 5A/Carbonic anhydrase 5A, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA5A by stopped-flow CO2 hydration method | B | 6.3 | pKi | 499 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| Carbonic anhydrase 5B, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA5B by stopped-flow CO2 hydration method | B | 6.49 | pKi | 322 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| Carbonic anhydrase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3025] [UniProtKB: P23280] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA6 by stopped-flow CO2 hydration method | B | 6.61 | pKi | 245 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 7/Carbonic anhydrase 7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166] | ||||||||
| ChEMBL | Inhibition of human recombinant full length CA7 by stopped-flow CO2 hydration method | B | 6.29 | pKi | 513 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| carbonic anhydrase 9/Carbonic anhydrase 9 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790] | ||||||||
| ChEMBL | Inhibition of human recombinant CA9 catalytic domain by stopped-flow CO2 hydration method | B | 6.38 | pKi | 420 | nM | Ki | Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014] |
| CDGSH iron-sulfur domain-containing protein 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795168] [UniProtKB: Q9NZ45] | ||||||||
| ChEMBL | Binding affinity to mitoNEET (unknown origin) by SPR method | B | 4.28 | pKd | 53000 | nM | Kd | Bioorg Med Chem Lett (2023) 89: 129310-129310 [PMID:37137430] |
| ChEMBL | Displacement of [3H]rosiglitazone from recombinant human C-terminal His-tagged MitoNEET cytosolic domain (32 to 108 residues) expressed in Escherichia coli BL21 by Cheng-Prusoff analysis | B | 5.64 | pKi | 2285 | nM | Ki | Bioorg Med Chem Lett (2016) 26: 5350-5353 [PMID:27687671] |
| ChEMBL | Displacement of [3H]rosiglitazone from recombinant human C-terminal His-tagged MitoNEET cytosolic domain (32 to 108 residues) expressed in Escherichia coli BL21 by scintillation proximity assay | B | 4.27 | pIC50 | 53460 | nM | IC50 | Bioorg Med Chem Lett (2016) 26: 5350-5353 [PMID:27687671] |
| GPR35/G-protein coupled receptor 35 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293267] [GtoPdb: 102] [UniProtKB: Q9HC97] | ||||||||
| ChEMBL | Displacement of [3H]PSB-13253 from human recombinant GPR35 exprssed in CHO cells by liquid scintillation counting analysis | B | 5.49 | pKi | 3270 | nM | Ki | J Med Chem (2013) 56: 7084-7099 [PMID:23888932] |
| ChEMBL | Agonist activity at C-terminal beta-galactosidase tagged human recombinant GPR35 expressed in CHO cells after 90 mins by beta-arrestin recruitment assay | B | 4.95 | pEC50 | 11200 | nM | EC50 | J Med Chem (2013) 56: 7084-7099 [PMID:23888932] |
| ChEMBL | Agonist activity at human GPR35 by Ca+2 release assay | B | 5.21 | pEC50 | 6200 | nM | EC50 | J Med Chem (2013) 56: 7084-7099 [PMID:23888932] |
| GtoPdb | - | - | 5.63 | pEC50 | - | - | - | Pharmacology (2012) 89: 13-7 [PMID:22236570] |
| Sodium/bile acid and sulphated solute cotransporter 1/Hepatic sodium/bile acid cotransporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5287] [GtoPdb: 959] [UniProtKB: Q14973] | ||||||||
| ChEMBL | TP_TRANSPORTER: inhibition of Taurocholate uptake in NTCP-expressing HeLa cells | F | 4.82 | pIC50 | 15000 | nM | IC50 | J Pharmacol Exp Ther (1999) 291: 1204-1209 [PMID:10565843] |
| Holo-[acyl-carrier-protein] synthase in Bacillus subtilis (strain 168) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4734] [UniProtKB: P96618] | ||||||||
| ChEMBL | Inhibitory activity against Bacillus subtilis AcpS by HTRF assay | B | 4.9 | pIC50 | 12700 | nM | IC50 | J Med Chem (2005) 48: 7960-7969 [PMID:16335920] |
| Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364] | ||||||||
| ChEMBL | Antiplasmodial activity against Plasmodium falciparum D10 after 72 hrs by SYBR green assay | F | 4.8 | pIC50 | 15848.93 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
| ChEMBL | Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
| ChEMBL | Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
| Organic anion transporter 1/Solute carrier family 22 member 6 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1777665] [GtoPdb: 1025] [UniProtKB: O35956] | ||||||||
| ChEMBL | TP_TRANSPORTER: inhibition of PAH uptake in Xenopus laevis oocytes | F | 5.02 | pKi | 9500 | nM | Ki | J Pharmacol Exp Ther (2000) 295: 261-265 [PMID:10991988] |
| OATP2A1/Solute carrier organic anion transporter family member 2A1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073680] [GtoPdb: 1223] [UniProtKB: Q00910] | ||||||||
| ChEMBL | TP_TRANSPORTER: inhibition of PGF2alpha uptake in PGT-expressing HeLa cells | F | 4.33 | pKi | 47000 | nM | Ki | Science (1995) 268: 866-869 [PMID:7754369] |
| Kidney-specific Na-K-Cl symporter in Human [GtoPdb: 968] [UniProtKB: Q13621] | ||||||||
| GtoPdb | - | - | 5.15 | pIC50 | - | - | - | Naunyn Schmiedebergs Arch Pharmacol (2002) 365: 193-9 [PMID:11882915] |
| Basolateral Na-K-Cl symporter in Human [GtoPdb: 969] [UniProtKB: P55011] | ||||||||
| GtoPdb | - | - | 5.13 | pIC50 | - | - | - | Naunyn Schmiedebergs Arch Pharmacol (2002) 365: 193-9 [PMID:11882915] |
ChEMBL data shown on this page come from version 36:
Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
