Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Human	-	1025	2q24.2	IFIH1	interferon induced with helicase C domain 1
Mouse	-	1025	2 C1.3	Ifih1	interferon induced with helicase C domain 1
Rat	-	1027	3q21	Ifih1	interferon induced with helicase C domain 1

Previous and Unofficial Names

MDA-5 | melanoma differentiation-associated gene 5

Database Links
Alphafold	Q9BYX4 (Hs), Q8R5F7 (Mm)
BRENDA	3.6.4.13
ChEMBL Target	CHEMBL4739862 (Hs)
Ensembl Gene	ENSG00000115267 (Hs), ENSMUSG00000026896 (Mm), ENSRNOG00000006227 (Rn)
Entrez Gene	64135 (Hs), 71586 (Mm), 499801 (Rn)
Human Protein Atlas	ENSG00000115267 (Hs)
KEGG Enzyme	3.6.4.13
KEGG Gene	hsa:64135 (Hs), mmu:71586 (Mm), rno:499801 (Rn)
OMIM	606951 (Hs)
Pharos	Q9BYX4 (Hs)
RefSeq Nucleotide	NM_022168 (Hs), NM_001164477 (Mm), NM_001109199 (Rn)
RefSeq Protein	NP_071451 (Hs), NP_001157949 (Mm), NP_001102669 (Rn)
UniProtKB	Q9BYX4 (Hs), Q8R5F7 (Mm)
Wikipedia	IFIH1 (Hs)

Enzyme Reaction
EC Number: 3.6.4.13 Description Reaction Reference RNA helicase; uses the energy from ATP to unwind RNA ATP + H(2)O <=> ADP + phosphate

Immunopharmacology Comments

MDA5 is an intracellualar RNA sensor. It recognizes longer double-stranded RNA sequences than RIG-1. In autoimmunity, MDA5 has been specifically linked with type I diabetes and anti-MDA5 autoantibodies can be detected in patients with certain connective tissue autoimmune conditions [1-3].

Immuno Process Associations

Immuno Process:  Inflammation

Immuno Process:  Immune regulation

Immuno Process:  Cytokine production & signalling

Immuno Process:  Cellular signalling

Physiological Consequences of Altering Gene Expression
Conventional dendritic cells (cDCs), macrophages and fibroblasts isolated from MDA5 null mice have impaired IFN induction after RNA virus infection. Plasmacytoid DCs are unaffected by MDA5 knockout, suggesting nucleic acid sensing TLRs are more relevant in these cells. Species:  Mouse Tissue:  cDCs, macrophages and fibroblasts. Technique:  References: