ChEMBL ligand: CHEMBL1055 (Chlortalidone, Chlortalidonum, Chlorthalidone, Chlorthalidone component of clorpres, Chlorthalidone component of combipres, Chlorthalidone component of demi-regroton, Chlorthalidone component of edarbyclor, Chlorthalidone component of kerledex, Chlorthalidone component of lopressidone, Chlorthalidone component of regroton, Chlorthalidone component of tenoretic, Clortalidona, G-33182, Hygroton, Natriuran, NSC-69200, Phthalamudine, Thalitone)
carbonic anhydrase 1/Carbonic anhydrase 1 in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 12/Carbonic anhydrase 12 in Human [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 13/Carbonic anhydrase 13 in Mouse [ChEMBL: CHEMBL2186] [GtoPdb: 2748] [UniProtKB: Q9D6N1] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 14/Carbonic anhydrase 14 in Human [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 2/Carbonic anhydrase 2 in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase 3 in Human [ChEMBL: CHEMBL2885] [UniProtKB: P07451] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 4/Carbonic anhydrase 4 in Human [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 5A/Carbonic anhydrase 5A, mitochondrial in Human [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase 5B, mitochondrial in Human [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase 6 in Human [ChEMBL: CHEMBL3025] [UniProtKB: P23280] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 7/Carbonic anhydrase 7 in Human [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 9/Carbonic anhydrase 9 in Human [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790] There should be some charts here, you may need to enable JavaScript!
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
carbonic anhydrase 1/Carbonic anhydrase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL	Inhibition of human recombinant full length CA1 by stopped-flow CO2 hydration method	B	6.46	pKi	348	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
GtoPdb	-	-	6.46	pKi	348	nM	Ki
ChEMBL	Known Inhibitors of Carbonic Anhydrase: Compounds which are CAIs are well known in the art, see for example, Pastorekova et al, Journal of Enzyme Inhibition and Medicinal Chemistry, 19(3), 199-229, 2004. Methods for determining the carbonic anhydrase inhibitory activity of a compound, i.e. whether a compound can be classified as a CAI, are known in the art and may usefully identify further compounds which may be suitable for use in the present invention. Exemplary methods and levels of activity required for consideration as CAIs are described in US 20020022245 and the references cited therein, the contents of which are incorporated herein by reference, in particular Supuran et al, European Journal of Medicinal Chemistry, 1998, 33, 577-594 and Scozzafava et al, Journal of Medicinal Chemistry, 1999, 42 25, 3690-3700).	B	6.59	pIC50	260	nM	IC50	US-10172837-B2. Insulin sensitisers and methods of treatment (2019)
carbonic anhydrase 12/Carbonic anhydrase 12 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570]
ChEMBL	Inhibition of human recombinant CA12 catalytic domain by stopped-flow CO2 hydration method	B	8.35	pKi	4.5	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
GtoPdb	-	-	8.35	pKi	4.5	nM	Ki	Bioorg Med Chem (2009) 17: 1214-21 [PMID:19119014]
carbonic anhydrase 13/Carbonic anhydrase 13 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2186] [GtoPdb: 2748] [UniProtKB: Q9D6N1]
ChEMBL	Inhibition of mouse recombinant full length CA13 by stopped-flow CO2 hydration method	B	7.82	pKi	15	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 14/Carbonic anhydrase 14 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7]
GtoPdb	-	-	5.38	pKi	4130	nM	Ki	Bioorg Med Chem (2009) 17: 1214-21 [PMID:19119014]
ChEMBL	Inhibition of human recombinant full length CA14 by stopped-flow CO2 hydration method	B	5.38	pKi	4130	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 2/Carbonic anhydrase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL	Inhibition of human recombinant full length CA2 by stopped-flow CO2 hydration method	B	6.86	pKi	138	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
ChEMBL	DRUGMATRIX: Carbonic Anhydrase II enzyme inhibition (substrate: 4-Nitrophenyl acetate (4-NPA))	B	6.17	pIC50	677	nM	IC50	DrugMatrix in vitro pharmacology data
Carbonic anhydrase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2885] [UniProtKB: P07451]
ChEMBL	Inhibition of human recombinant full length CA3 by stopped-flow CO2 hydration method	B	4.96	pKi	11000	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 4/Carbonic anhydrase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748]
ChEMBL	Inhibition of human recombinant full length CA4 by stopped-flow CO2 hydration method	B	6.71	pKi	196	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
GtoPdb	-	-	6.71	pKi	196	nM	Ki	Bioorg Med Chem (2009) 17: 1214-21 [PMID:19119014]
carbonic anhydrase 5A/Carbonic anhydrase 5A, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218]
ChEMBL	Inhibition of human recombinant full length CA5A by stopped-flow CO2 hydration method	B	6.04	pKi	917	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
Carbonic anhydrase 5B, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0]
ChEMBL	Inhibition of human recombinant full length CA5B by stopped-flow CO2 hydration method	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
Carbonic anhydrase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3025] [UniProtKB: P23280]
ChEMBL	Inhibition of human recombinant full length CA6 by stopped-flow CO2 hydration method	B	5.87	pKi	1347	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 7/Carbonic anhydrase 7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166]
GtoPdb	-	-	8.55	pKi	2.8	nM	Ki	Bioorg Med Chem (2009) 17: 1214-21 [PMID:19119014]
ChEMBL	Inhibition of human recombinant full length CA7 by stopped-flow CO2 hydration method	B	8.55	pKi	2.8	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
carbonic anhydrase 9/Carbonic anhydrase 9 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790]
ChEMBL	Inhibition of human recombinant CA9 catalytic domain by stopped-flow CO2 hydration method	B	7.64	pKi	23	nM	Ki	Bioorg Med Chem (2009) 17: 1214-1221 [PMID:19119014]
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20]
GtoPdb	Equilibrium dissociation constant determined in the presence of DCA	-	6.17	pKd	680	nM	Kd	Cell Chem Biol (2025) 32: 449-462.e5 [PMID:39999832]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]