ChEMBL ligand: CHEMBL325041 (Bortezomib, Bortezomib accord, Bortezomib hydrate, LDP-341, NSC-681239, PS-341, Velcade)
proteasome 20S subunit beta 1/proteasome 20S subunit beta 8/proteasome 20S subunit beta 9/proteasome 20S subunit beta 6/proteasome 20S subunit beta 5/proteasome 20S subunit beta 2/20S proteasome in Human [ChEMBL: CHEMBL3831201] [GtoPdb: 2404, 2408, 2409, 2407, 2406, 2405] [UniProtKB: A5LHX3, O14818, P20618, P25786, P25787, P25788, P25789, P28062, P28065, P28066, P28070, P28072, P28074, P40306, P49720, P49721, P60900, Q8TAA3, Q99436] There should be some charts here, you may need to enable JavaScript!
proteasome 26S subunit, non-ATPase 14/proteasome 20S subunit beta 1/proteasome 20S subunit beta 8/proteasome 20S subunit beta 9/proteasome 20S subunit beta 6/proteasome 20S subunit beta 5/proteasome 20S subunit beta 2/26S proteasome in Human [ChEMBL: CHEMBL2364701] [GtoPdb: 2410, 2404, 2408, 2409, 2407, 2406, 2405] [UniProtKB: A5LHX3, O00231, O00232, O00487, O14818, O43242, P17980, P20618, P25786, P25787, P25788, P25789, P28062, P28065, P28066, P28070, P28072, P28074, P35998, P40306, P43686, P48556, P49720, P49721, P51665, P55036, P60896, P60900, P62191, P62195, P62333, Q13200, Q15008, Q16186, Q8TAA3, Q99436, Q99460, Q9UNM6] There should be some charts here, you may need to enable JavaScript!
Alpha-chymotrypsin in Bovine [ChEMBL: CHEMBL3314] [UniProtKB: P00766] There should be some charts here, you may need to enable JavaScript!
ATP-dependent Clp protease proteolytic subunit in Staphylococcus aureus (strain NCTC 8325) [ChEMBL: CHEMBL1932910] [UniProtKB: Q2G036] There should be some charts here, you may need to enable JavaScript!
Beta-chymotrypsin in Human [ChEMBL: CHEMBL4796] [UniProtKB: P17538] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase 15 in Mouse [ChEMBL: CHEMBL5973] [UniProtKB: Q99N23] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 1/Carbonic anhydrase I in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 2/Carbonic anhydrase II in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase III in Human [ChEMBL: CHEMBL2885] [UniProtKB: P07451] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 4/Carbonic anhydrase IV in Human [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 9/Carbonic anhydrase IX in Human [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 5A/Carbonic anhydrase VA in Human [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase VB in Human [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase VI in Human [ChEMBL: CHEMBL3025] [UniProtKB: P23280] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 7/Carbonic anhydrase VII in Human [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 12/Carbonic anhydrase XII in Human [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 13/Carbonic anhydrase XIII in Human [ChEMBL: CHEMBL3912] [GtoPdb: 2748] [UniProtKB: Q8N1Q1] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 14/Carbonic anhydrase XIV in Human [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7] There should be some charts here, you may need to enable JavaScript!
cathepsin B/Cathepsin B in Human [ChEMBL: CHEMBL4072] [GtoPdb: 2343] [UniProtKB: P07858] There should be some charts here, you may need to enable JavaScript!
cathepsin G/Cathepsin G in Human [ChEMBL: CHEMBL4071] [GtoPdb: 2348] [UniProtKB: P08311] There should be some charts here, you may need to enable JavaScript!
chymase 1/Chymase in Human [ChEMBL: CHEMBL4068] [GtoPdb: 2340] [UniProtKB: P23946] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 2/Histone deacetylase 2 in Human [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3 in Human [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 8/Histone deacetylase 8 in Human [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41] There should be some charts here, you may need to enable JavaScript!
elastase, neutrophil expressed/Leukocyte elastase in Human [ChEMBL: CHEMBL248] [GtoPdb: 2358] [UniProtKB: P08246] There should be some charts here, you may need to enable JavaScript!
lon peptidase 1, mitochondrial/Lon protease homolog, mitochondrial in Human [ChEMBL: CHEMBL4879436] [GtoPdb: 3180] [UniProtKB: P36776] There should be some charts here, you may need to enable JavaScript!
Cathepsin A/Lysosomal protective protein in Human [ChEMBL: CHEMBL6115] [GtoPdb: 1581] [UniProtKB: P10619] There should be some charts here, you may need to enable JavaScript!
Nuclear factor NF-kappa-B complex in Human [ChEMBL: CHEMBL2094258] [UniProtKB: P19838, Q00653, Q04206] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 1/Proteasome component C5 in Human [ChEMBL: CHEMBL4208] [GtoPdb: 2404] [UniProtKB: P20618] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 2/Proteasome Macropain subunit in Human [ChEMBL: CHEMBL3492] [GtoPdb: 2405] [UniProtKB: P49721] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 5/Proteasome Macropain subunit MB1 in Human [ChEMBL: CHEMBL4662] [GtoPdb: 2406] [UniProtKB: P28074] There should be some charts here, you may need to enable JavaScript!
Proteasome subunit beta type-10 in Human [ChEMBL: CHEMBL3317334] [UniProtKB: P40306] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 8/Proteasome subunit beta type-8 in Human [ChEMBL: CHEMBL5620] [GtoPdb: 2408] [UniProtKB: P28062] proteasome 20S subunit beta 8/Proteasome subunit beta type-8 in Mouse [ChEMBL: CHEMBL1944492] [GtoPdb: 2408] [UniProtKB: P28063] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 9/Proteasome subunit beta type-9 in Human [ChEMBL: CHEMBL1944495] [GtoPdb: 2409] [UniProtKB: P28065] There should be some charts here, you may need to enable JavaScript!
coagulation factor II, thrombin/Thrombin in Human [ChEMBL: CHEMBL204] [GtoPdb: 2362] [UniProtKB: P00734] There should be some charts here, you may need to enable JavaScript!
Uncharacterized protein Rv1284/MT1322 in Mycobacterium tuberculosis [ChEMBL: CHEMBL5631] [UniProtKB: P9WPJ7] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum proteasome subunit beta type-5 in Plasmodium falciparum W2 [GtoPdb: 3088] Plasmodium falciparum proteasome subunit beta type-5 in Plasmodium falciparum [GtoPdb: 3088] Plasmodium falciparum proteasome subunit beta type-5 in Plasmodium falciparum 3D7 [GtoPdb: 3088] [UniProtKB: Q8IJT1] Plasmodium falciparum proteasome subunit beta type-5 in Plasmodium falciparum Dd2 [GtoPdb: 3088] Plasmodium falciparum proteasome subunit beta type-5 in Plasmodium falciparum HB3 [GtoPdb: 3088] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
proteasome 20S subunit beta 1/proteasome 20S subunit beta 8/proteasome 20S subunit beta 9/proteasome 20S subunit beta 6/proteasome 20S subunit beta 5/proteasome 20S subunit beta 2/20S proteasome in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3831201] [GtoPdb: 2404, 2408, 2409, 2407, 2406, 2405] [UniProtKB: A5LHX3, O14818, P20618, P25786, P25787, P25788, P25789, P28062, P28065, P28066, P28070, P28072, P28074, P40306, P49720, P49721, P60900, Q8TAA3, Q99436]
ChEMBL	Inhibition of human erythrocytes 20S proteasome using QXL520-YRGITCSGRQK(5-FAM)-NH2 fluorogenic peptide as substrate preincubated for 10 mins followed by substrate addition and measured after 60 mins by HTRF assay	B	7.01	pIC50	97	nM	IC50	J Med Chem (2021) 64: 4857-4869 [PMID:33821636]
GtoPdb	-	-	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3220-4 [PMID:19428245]
ChEMBL	Inhibition of human 20S proteasome preincubated for 15 mins followed by substrate addition	B	7.97	pIC50	10.8	nM	IC50	Eur J Med Chem (2019) 164: 602-614 [PMID:30639896]
proteasome 26S subunit, non-ATPase 14/proteasome 20S subunit beta 1/proteasome 20S subunit beta 8/proteasome 20S subunit beta 9/proteasome 20S subunit beta 6/proteasome 20S subunit beta 5/proteasome 20S subunit beta 2/26S proteasome in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2364701] [GtoPdb: 2410, 2404, 2408, 2409, 2407, 2406, 2405] [UniProtKB: A5LHX3, O00231, O00232, O00487, O14818, O43242, P17980, P20618, P25786, P25787, P25788, P25789, P28062, P28065, P28066, P28070, P28072, P28074, P35998, P40306, P43686, P48556, P49720, P49721, P51665, P55036, P60896, P60900, P62191, P62195, P62333, Q13200, Q15008, Q16186, Q8TAA3, Q99436, Q99460, Q9UNM6]
ChEMBL	Inhibition of trypsin-like activity of human 20S proteasome using Ac-RLR-AMC as substrate	B	5.7	pIC50	2000	nM	IC50	J Med Chem (2014) 57: 2726-2735 [PMID:24524217]
ChEMBL	Inhibition of caspase-like activity of human 20S proteasome using Ac-nLPnLD-AMC as substrate	B	6.92	pIC50	120	nM	IC50	J Med Chem (2014) 57: 2726-2735 [PMID:24524217]
GtoPdb	-	-	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3220-4 [PMID:19428245]
ChEMBL	Inhibition of chymotrypsin-like enzyme activity of purified human 20S proteasome using Suc-Leu-Leu-Val-Tyr-AMC as substrate by fluorescence method	B	8.15	pIC50	7.14	nM	IC50	Bioorg Med Chem (2014) 22: 2955-2965 [PMID:24767818]
ChEMBL	Inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-LLVY-AMC as substrate	B	8.15	pIC50	7	nM	IC50	J Med Chem (2014) 57: 2726-2735 [PMID:24524217]
ChEMBL	Inhibition of human 20S proteasome using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 90 mins by fluorescence assay	B	8.59	pIC50	2.56	nM	IC50	Medchemcomm (2011) 2: 1083-1086
Alpha-chymotrypsin in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3314] [UniProtKB: P00766]
ChEMBL	Inhibition of bovine pancreatic alpha-chymotrypsin using Suc-Leu-Leu-Val-Tyr-AMC as substrate by fluorescence assay	B	5.62	pKi	2400	nM	Ki	Eur J Med Chem (2014) 83: 1-14 [PMID:24946214]
ATP-dependent Clp protease proteolytic subunit in Staphylococcus aureus (strain NCTC 8325) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1932910] [UniProtKB: Q2G036]
ChEMBL	Inhibition of N-terminal His6-sumo-tagged full length Staphylococcus aureus ClpP expressed in Escherichia coli BL2 (DE3) pre-incubated for 10 mins before Suc-LY-AMC addition and measured after 1 hr by fluorescence based assay	B	5.28	pIC50	5300	nM	IC50	J Med Chem (2020) 63: 3104-3119 [PMID:32031798]
ChEMBL	Inhibition of ClpP in methicillin-resistant Staphylococcus aureus ATCC 33591 assessed as reduction in bacteria-induced hemolysis of sheep erythrocytes by measuring hemoglobin release incubated for 15 mins	B	4.63	pEC50	23500	nM	EC50	J Med Chem (2020) 63: 3104-3119 [PMID:32031798]
Beta-chymotrypsin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4796] [UniProtKB: P17538]
ChEMBL	Inhibitory activity against human Chymotrypsinogen	B	6.49	pKi	320	nM	Ki	Bioorg Med Chem Lett (1998) 8: 333-338 [PMID:9871680]
Carbonic anhydrase 15 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5973] [UniProtKB: Q99N23]
ChEMBL	Inhibition of mouse CA15 by stopped-flow CO2 hydrase assay	B	5.57	pKi	2680	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 1/Carbonic anhydrase I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL	Inhibition of human carbonic anhydrase-1 preincubated for 15 mins by stopped-flow CO2 hydration assay	B	5.89	pKi	1290	nM	Ki	Bioorg Med Chem (2016) 24: 4406-4409 [PMID:27469982]
ChEMBL	Inhibition of human CA1 by stopped-flow CO2 hydrase assay	B	5.89	pKi	1290	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 2/Carbonic anhydrase II in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL	Inhibition of human carbonic anhydrase-2 preincubated for 15 mins by stopped-flow CO2 hydration assay	B	5.94	pKi	1160	nM	Ki	Bioorg Med Chem (2016) 24: 4406-4409 [PMID:27469982]
ChEMBL	Inhibition of human CA2 by stopped-flow CO2 hydrase assay	B	5.94	pKi	1160	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
Carbonic anhydrase III in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2885] [UniProtKB: P07451]
ChEMBL	Inhibition of human CA3 by stopped-flow CO2 hydrase assay	B	5.34	pKi	4570	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 4/Carbonic anhydrase IV in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3729] [GtoPdb: 2599] [UniProtKB: P22748]
ChEMBL	Inhibition of human CA4 by stopped-flow CO2 hydrase assay	B	5.19	pKi	6510	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 9/Carbonic anhydrase IX in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3594] [GtoPdb: 3055] [UniProtKB: Q16790]
ChEMBL	Inhibition of human CA9 by stopped-flow CO2 hydrase assay	B	5.47	pKi	3380	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 5A/Carbonic anhydrase VA in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4789] [GtoPdb: 3093] [UniProtKB: P35218]
ChEMBL	Inhibition of human CA5A by stopped-flow CO2 hydrase assay	B	5.16	pKi	6920	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
Carbonic anhydrase VB in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3969] [UniProtKB: Q9Y2D0]
ChEMBL	Inhibition of human CA5B by stopped-flow CO2 hydrase assay	B	5.17	pKi	6830	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
Carbonic anhydrase VI in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3025] [UniProtKB: P23280]
ChEMBL	Inhibition of human CA6 by stopped-flow CO2 hydrase assay	B	5.36	pKi	4380	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 7/Carbonic anhydrase VII in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2326] [GtoPdb: 2749] [UniProtKB: P43166]
ChEMBL	Inhibition of human CA7 by stopped-flow CO2 hydrase assay	B	5.16	pKi	6940	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 12/Carbonic anhydrase XII in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3242] [GtoPdb: 2747] [UniProtKB: O43570]
ChEMBL	Inhibition of human CA12 by stopped-flow CO2 hydrase assay	B	5.23	pKi	5870	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 13/Carbonic anhydrase XIII in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3912] [GtoPdb: 2748] [UniProtKB: Q8N1Q1]
ChEMBL	Inhibition of human CA13 by stopped-flow CO2 hydrase assay	B	5.07	pKi	8450	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
carbonic anhydrase 14/Carbonic anhydrase XIV in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3510] [GtoPdb: 2598] [UniProtKB: Q9ULX7]
ChEMBL	Inhibition of human CA14 by stopped-flow CO2 hydrase assay	B	5.48	pKi	3280	nM	Ki	Bioorg Med Chem (2017) 25: 5064-5067 [PMID:28277280]
cathepsin B/Cathepsin B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4072] [GtoPdb: 2343] [UniProtKB: P07858]
ChEMBL	Inhibition of recombinant cathepsin B (unknown origin) using Z-Leu-Arg-AMC as substrate by spectrofluorometer analysis	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2014) 57: 2726-2735 [PMID:24524217]
cathepsin G/Cathepsin G in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4071] [GtoPdb: 2348] [UniProtKB: P08311]
ChEMBL	Inhibitory activity against human cathepsin G	B	6.2	pKi	630	nM	Ki	Bioorg Med Chem Lett (1998) 8: 333-338 [PMID:9871680]
ChEMBL	Inhibition of recombinant cathepsin G (unknown origin) using Suc-Ala-Ala-Pro-Phe-AMC as substrate by spectrofluorometer analysis	B	5.51	pIC50	3100	nM	IC50	J Med Chem (2014) 57: 2726-2735 [PMID:24524217]
ChEMBL	Inhibition of human cathepsin G	B	6.28	pIC50	520	nM	IC50	J Med Chem (2008) 51: 1068-1072 [PMID:18247547]
chymase 1/Chymase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4068] [GtoPdb: 2340] [UniProtKB: P23946]
ChEMBL	Inhibition of human chymase	B	5.92	pIC50	1190	nM	IC50	J Med Chem (2008) 51: 1068-1072 [PMID:18247547]
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Inhibition of HDAC1 in human RPMI-8226 cells incubated for 2 hrs by ELISA	B	6.21	pIC50	620