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ChEMBL ligand: CHEMBL1544 (3,5,3'-triiodothyronine, l-, Cyronine, Cytomel, Euthroid-0.5, Euthroid-1, Euthroid-2, Euthroid-3, Levothyroxine sodium related compound liothyronine, Liothyronine, NSC-80203, Rathyronine, Rathyronine, (s)-, Tertroxin, Thyrolar-0.25, Thyrolar-0.5, Thyrolar-1, Thyrolar-2, Thyrolar-3, Thyrolar-5, Triiodothyronine (T3 Or Liothyronine, Active) (6-11%), Triostat) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] | ||||||||
ChEMBL | DRUGMATRIX: Adenosine A1 radioligand binding (ligand: DPCPX) | B | 6.62 | pKi | 239.7 | nM | Ki | DrugMatrix in vitro pharmacology data |
ChEMBL | DRUGMATRIX: Adenosine A1 radioligand binding (ligand: DPCPX) | B | 6.39 | pIC50 | 410.9 | nM | IC50 | DrugMatrix in vitro pharmacology data |
Androgen receptor/Androgen Receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1871] [GtoPdb: 628] [UniProtKB: P10275] | ||||||||
ChEMBL | Transcriptional activity at human androgen receptor BF3 site stably transfected in eGFP-expressing human LNCAP cells after 5 days by fluorometric analysis | B | 4.3 | pIC50 | >50000 | nM | IC50 | J Med Chem (2011) 54: 8563-8573 [PMID:22047606] |
GABAA receptor π subunit/GABAA receptor δ subunit/GABAA receptor α1 subunit/GABAA receptor β1 subunit/GABAA receptor γ2 subunit/GABAA receptor β3 subunit/GABAA receptor α5 subunit/GABAA receptor α3 subunit/GABAA receptor α2 subunit/GABAA receptor β2 subunit/GABAA receptor α4 subunit/GABAA receptor ε subunit/GABAA receptor α6 subunit/GABAA receptor γ1 subunit/GABAA receptor γ3 subunit/GABAA receptor θ subunit/GABA-A receptor; anion channel in Human (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2093872] [GtoPdb: 419, 416, 404, 410, 414, 412, 408, 406, 405, 411, 407, 417, 409, 413, 415, 418] [UniProtKB: O00591, O14764, P14867, P18505, P18507, P28472, P31644, P34903, P47869, P47870, P48169, P78334, Q16445, Q8N1C3, Q99928, Q9UN88] | ||||||||
ChEMBL | Direct channel-gating action blocked by picrotoxin | B | 4.64 | pIC50 | 23000 | nM | IC50 | J Med Chem (2000) 43: 1427-1447 [PMID:10780899] |
GABAA receptor π subunit/GABAA receptor β1 subunit/GABAA receptor δ subunit/GABAA receptor γ2 subunit/GABAA receptor α5 subunit/GABAA receptor α3 subunit/GABAA receptor γ1 subunit/GABAA receptor α2 subunit/GABAA receptor α4 subunit/GABAA receptor γ3 subunit/GABAA receptor α6 subunit/GABAA receptor α1 subunit/GABAA receptor β3 subunit/GABAA receptor β2 subunit/GABAA receptor ε subunit/GABA-A receptor; anion channel in Rat (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL1907607] [GtoPdb: 419, 410, 416, 414, 408, 406, 413, 405, 407, 415, 409, 404, 412, 411, 417] [UniProtKB: O09028, P15431, P18506, P18508, P19969, P20236, P23574, P23576, P28471, P28473, P30191, P62813, P63079, P63138, Q91ZM7, Q9ES14] | ||||||||
ChEMBL | Non-competitive inhibitory activity against recombinant Gamma-aminobutyric acid A (GABA-A) receptor | B | 5.15 | pEC50 | 7000 | nM | EC50 | J Med Chem (2000) 43: 1427-1447 [PMID:10780899] |
Peroxisome proliferator-activated receptor-γ/Peroxisome proliferator-activated receptor gamma in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL235] [GtoPdb: 595] [UniProtKB: P37231] | ||||||||
ChEMBL | Binding affinity to recombinant PPARgamma LBD (unknown origin) by isothermal titration calorimetry | B | 6.15 | pKd | 710 | nM | Kd | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
ChEMBL | Agonist activity at recombinant human pFA-CMV fused PPARgamma expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay | B | 5.24 | pEC50 | 5800 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
Proliferating cell nuclear antigen in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2346488] [UniProtKB: P12004] | ||||||||
ChEMBL | Inhibition of human recombinant PCNA interaction with PIP box protein N-5-carboxyfluorescein-SAVLQKKITDYFHPKK after 30 mins by fluorescence polarization assay | B | 5.44 | pIC50 | 3600 | nM | IC50 | Bioorg Med Chem (2013) 21: 1972-1977 [PMID:23395113] |
Solute carrier organic anion transporter family member 1A4 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1781860] [UniProtKB: O35913] | ||||||||
ChEMBL | TP_TRANSPORTER: inhibition of Digoxin uptake in Xenopus laevis oocytes | F | 4.3 | pIC50 | 50400 | nM | IC50 | J Pharmacol Exp Ther (2001) 299: 261-267 [PMID:11561088] |
OATP1C1/Solute carrier organic anion transporter family member 1C1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073688] [GtoPdb: 1222] [UniProtKB: Q9ERB5] | ||||||||
ChEMBL | TP_TRANSPORTER: inhibition of L-T4 uptake in Oatp14-expressing HEK293 cells | F | 4.62 | pKi | 24200 | nM | Ki | Endocrinology (2004) 145: 4384-4391 [PMID:15166123] |
coagulation factor II, thrombin/Thrombin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL204] [GtoPdb: 2362] [UniProtKB: P00734] | ||||||||
ChEMBL | Agonist activity at human THRbeta expressed in CHO cells after 24 hrs by luciferase assay | B | 8.21 | pEC50 | 6.2 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
ChEMBL | Agonist activity at GST-labelled THRbeta ( 202 to 461 residues) (unknown origin) incubated for 2 hrs in presence SRC1-2 co-activator peptide by Alphascreen assay | B | 8.92 | pEC50 | 1.2 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
ChEMBL | Agonist activity at human THRbeta expressed in green monkey CV1 cells after 24 hrs by luciferase assay | B | 8.92 | pEC50 | 1.2 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
Thyroid hormone receptor-α/Thyroid hormone receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1860] [GtoPdb: 588] [UniProtKB: P10827] | ||||||||
ChEMBL | Binding affinity towards thyroid hormone receptor (hTR alpha 1) | B | 9.77 | pKd | 0.17 | nM | Kd | Bioorg Med Chem Lett (2001) 11: 2821-2825 [PMID:11597408] |
GtoPdb | - | - | 10 | pKd | - | - | - | J Med Chem (2002) 45: 3310-20 [PMID:12109914] |
ChEMBL | Binding affinity against human Thyroid hormone receptor alpha-1 (hTRalpha1) using radiolabeled T3 | B | 10 | pKd | 0.1 | nM | Kd | J Med Chem (2002) 45: 3310-3320 [PMID:12109914] |
ChEMBL | Binding affinity of compound was determined against Thyroid hormone receptor alpha1 | B | 10.22 | pKd | 0.06 | nM | Kd | J Med Chem (2003) 46: 3152-3161 [PMID:12825953] |
ChEMBL | Displacement of [125I]T3 from human TRalpha receptor | B | 10.24 | pKd | 0.06 | nM | Kd | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
ChEMBL | Displacement of [125I]T3 from human recombinant thyroid harmone receptor alpha after 16 to 48 hrs by gamma-ray detection | B | 8.63 | pKi | 2.33 | nM | Ki | Bioorg Med Chem (2012) 20: 3622-3634 [PMID:22542282] |
ChEMBL | Displacement of [125I]-T3 from human TRalpha expressed in insect cells after 16 to 48 hrs by gamma-counting | B | 8.63 | pKi | 2.33 | nM | Ki | Bioorg Med Chem (2013) 21: 592-607 [PMID:23276448] |
ChEMBL | Displacement of [125I]3,5,3'-triiodo-L-thyronine from His-tagged human recombinant TRalpha1 by scintillation proximity assay | B | 9.48 | pKi | 0.33 | nM | Ki | J Med Chem (2008) 51: 7075-7093 [PMID:18975928] |
ChEMBL | Displacement of [125I]T3 from recombinant thyroid hormone receptor alpha expressed in sf9 cells by scintillation proximity assay | B | 9.66 | pKi | 0.22 | nM | Ki | Proc Natl Acad Sci U S A (2007) 104: 15490-15495 [PMID:17878314] |
ChEMBL | Displacement of [125I]-Triiodothyronine from human recombinant TRalpha1 ligand binding domain after 2 to 3 hrs by beta counting | B | 8.49 | pIC50 | 3.2 | nM | IC50 | Bioorg Med Chem (2014) 22: 488-498 [PMID:24290064] |
ChEMBL | Inhibitory concentration against cloned human thyroid hormone receptor alpha 1 | B | 9.62 | pIC50 | 0.24 | nM | IC50 | Bioorg Med Chem Lett (2005) 15: 4579-4584 [PMID:16099653] |
ChEMBL | Binding affinity at human thyroid hormone receptor alpha 1 expressed in CHOK1 cells | B | 9.62 | pIC50 | 0.24 | nM | IC50 | Bioorg Med Chem Lett (2007) 17: 4131-4134 [PMID:17543524] |
ChEMBL | Agonist activity at recombinant His6-tagged THR-alpha (unknown origin) expressed in Escherichia coli BL21(DE3) co-expressing RXR preincubated for 30 mins assessed as biotin-GRIP1 peptide recruitment by HTRF assay | B | 8 | pEC50 | 10 | nM | EC50 | J Med Chem (2014) 57: 3912-3923 [PMID:24712661] |
ChEMBL | Agonist activity at human THRalpha expressed in CHO cells after 24 hrs by luciferase assay | B | 8.47 | pEC50 | 3.4 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
ChEMBL | Effect on human TRalpha transactivation activity in U2OS cells by luciferase reporter assay | B | 8.62 | pEC50 | 2.4 | nM | EC50 | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
ChEMBL | Effect on human TRalpha transactivation activity in HeLa cells by luciferase reporter assay | B | 8.62 | pEC50 | 2.4 | nM | EC50 | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
ChEMBL | Agonist activity at human recombinant TRalpha1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay | B | 8.68 | pEC50 | 2.1 | nM | EC50 | Bioorg Med Chem (2014) 22: 488-498 [PMID:24290064] |
ChEMBL | Half-maximum activation of human Thyroid hormone receptor alpha-1 (hTRalpha1) | B | 8.7 | pEC50 | 2 | nM | EC50 | J Med Chem (2002) 45: 3310-3320 [PMID:12109914] |
ChEMBL | Effective concentration binding towards TRalpha in E25B2 cells (agonistic activity) | B | 8.7 | pEC50 | 2 | nM | EC50 | Bioorg Med Chem Lett (2003) 13: 379-382 [PMID:12565933] |
ChEMBL | Agonist activity at human THRalpha expressed in baculovirus infected SF9 cells after 15 mins by gel electrophoresis | B | 9.3 | pEC50 | 0.5 | nM | EC50 | J Med Chem (2020) 63: 6727-6740 [PMID:32356658] |
ChEMBL | Agonist activity at human thyroid hormone receptor alpha expressed in HEK293 cells by luciferase reporter gene assay | B | 9.39 | pEC50 | 0.41 | nM | EC50 | Bioorg Med Chem Lett (2014) 24: 5265-5267 [PMID:25305688] |
Thyroid hormone receptor-β/Thyroid hormone receptor beta-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1947] [GtoPdb: 589] [UniProtKB: P10828] | ||||||||
ChEMBL | Binding affinity towards thyroid hormone receptor (hTR beta 1) | B | 9.85 | pKd | 0.14 | nM | Kd | Bioorg Med Chem Lett (2001) 11: 2821-2825 [PMID:11597408] |
ChEMBL | Binding affinity against human Thyroid hormone receptor beta 1 (hTRbeta1) using radiolabeled T3 | B | 10 | pKd | 0.1 | nM | Kd | J Med Chem (2002) 45: 3310-3320 [PMID:12109914] |
ChEMBL | Binding affinity of compound was determined against thyroid hormone receptor beta 1 | B | 10.06 | pKd | 0.09 | nM | Kd | J Med Chem (2003) 46: 3152-3161 [PMID:12825953] |
ChEMBL | Displacement of [125I]T3 from human TRbeta receptor | B | 10.09 | pKd | 0.08 | nM | Kd | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
GtoPdb | - | - | 10.1 | pKd | - | - | - | J Med Chem (2002) 45: 3310-20 [PMID:12109914] |
ChEMBL | Displacement of [125I]T3 from human recombinant thyroid harmone receptor beta after 16 to 48 hrs by gamma-ray detection | B | 8.64 | pKi | 2.29 | nM | Ki | Bioorg Med Chem (2012) 20: 3622-3634 [PMID:22542282] |
ChEMBL | Displacement of [125I]-T3 from human TRbeta expressed in insect cells after 16 to 48 hrs by gamma-counting | B | 8.64 | pKi | 2.29 | nM | Ki | Bioorg Med Chem (2013) 21: 592-607 [PMID:23276448] |
ChEMBL | Displacement of [125I]3,5,3'-triiodo-L-thyronine His-tagged human recombinant TRbeta1 by scintillation proximity assay | B | 9.17 | pKi | 0.68 | nM | Ki | J Med Chem (2008) 51: 7075-7093 [PMID:18975928] |
ChEMBL | Displacement of [125I]T3 from recombinant thyroid hormone receptor beta expressed in sf9 cells by scintillation proximity assay | B | 9.26 | pKi | 0.55 | nM | Ki | Proc Natl Acad Sci U S A (2007) 104: 15490-15495 [PMID:17878314] |
ChEMBL | Inhibitory activity against [125I]T3 binding to human TRbeta1 receptor | B | 10.1 | pKi | 0.08 | nM | Ki | Bioorg Med Chem Lett (2003) 13: 379-382 [PMID:12565933] |
ChEMBL | Displacement of [125I]-Triiodothyronine from human recombinant TRbeta1 ligand binding domain after 2 to 3 hrs by beta counting | B | 8.54 | pIC50 | 2.9 | nM | IC50 | Bioorg Med Chem (2014) 22: 488-498 [PMID:24290064] |
ChEMBL | Inhibitory concentration against cloned human thyroid hormone receptor beta 1 | B | 9.59 | pIC50 | 0.26 | nM | IC50 | Bioorg Med Chem Lett (2005) 15: 4579-4584 [PMID:16099653] |
ChEMBL | Binding affinity at human thyroid hormone receptor beta-1 expressed in CHOK1 cells | B | 9.59 | pIC50 | 0.26 | nM | IC50 | Bioorg Med Chem Lett (2007) 17: 4131-4134 [PMID:17543524] |
ChEMBL | Inhibition of human thyroid hormone receptor beta 1 | B | 9.59 | pIC50 | 0.26 | nM | IC50 | Bioorg Med Chem (2007) 15: 5251-5261 [PMID:17524652] |
ChEMBL | Agonist activity at recombinant His6-tagged THR-beta (unknown origin) expressed in Escherichia coli BL21(DE3) co-expressing RXR preincubated for 30 mins assessed as biotin-GRIP1 peptide recruitment by HTRF assay | B | 7.82 | pEC50 | 15 | nM | EC50 | J Med Chem (2014) 57: 3912-3923 [PMID:24712661] |
ChEMBL | Effect on human TRbeta transactivation activity in U2OS cells by luciferase reporter assay | B | 7.96 | pEC50 | 11 | nM | EC50 | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
ChEMBL | Effect on human TRbeta transactivation activity in HeLa cells by luciferase reporter assay | B | 8.62 | pEC50 | 2.4 | nM | EC50 | Bioorg Med Chem (2008) 16: 762-770 [PMID:17988877] |
ChEMBL | Half-maximum activation of human Thyroid hormone receptor beta 1 (hTRbeta1) | B | 8.7 | pEC50 | 2 | nM | EC50 | J Med Chem (2002) 45: 3310-3320 [PMID:12109914] |
ChEMBL | Effective concentration binding towards TR-beta-1 in E25B2 cells (agonistic activity) | B | 8.7 | pEC50 | 2 | nM | EC50 | Bioorg Med Chem Lett (2003) 13: 379-382 [PMID:12565933] |
ChEMBL | Agonist activity at human recombinant TRbeta1 transfected in CV-1 cells after 8 to 10 hrs by alkaline phosphatase reporter gene assay | B | 8.7 | pEC50 | 2 | nM | EC50 | Bioorg Med Chem (2014) 22: 488-498 [PMID:24290064] |
Thyroid hormone receptor-β/Thyroid hormone receptor beta-1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3917] [GtoPdb: 589] [UniProtKB: P18113] | ||||||||
ChEMBL | In vitro inhibition of bound [125I]L-T3 rat plasma membrane 3,5,3'' L-triiodothyronine receptor | B | 6.3 | pIC50 | 500 | nM | IC50 | J Med Chem (1995) 38: 695-707 [PMID:7861417] |
ChEMBL | In vitro inhibition of bound [125I]L-T3 rat plasma membrane 3,5,3'' L-triiodothyronine receptor | B | 7 | pIC50 | 100 | nM | IC50 | J Med Chem (1995) 38: 695-707 [PMID:7861417] |
ChEMBL | In vitro inhibition of the bound [125I]L-T3 rat liver nuclear L-triiodothyronine receptor | B | 8.36 | pIC50 | 4.4 | nM | IC50 | J Med Chem (1995) 38: 695-707 [PMID:7861417] |
ChEMBL | In vitro inhibition of the bound [125I]L-T3 rat liver nuclear L-triiodothyronine receptor | B | 8.74 | pIC50 | 1.8 | nM | IC50 | J Med Chem (1995) 38: 695-707 [PMID:7861417] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]