ChEMBL ligand: CHEMBL371214 (JWH-133)
CB1 receptor/Cannabinoid CB1 receptor in Human [ChEMBL: CHEMBL218] [GtoPdb: 56] [UniProtKB: P21554] CB1 receptor/Cannabinoid CB1 receptor in Mouse [ChEMBL: CHEMBL3037] [GtoPdb: 56] [UniProtKB: P47746] CB1 receptor/Cannabinoid CB1 receptor in Rat [ChEMBL: CHEMBL3571] [GtoPdb: 56] [UniProtKB: P20272] There should be some charts here, you may need to enable JavaScript!
CB2 receptor/Cannabinoid CB2 receptor in Human [ChEMBL: CHEMBL253] [GtoPdb: 57] [UniProtKB: P34972] CB2 receptor/Cannabinoid CB2 receptor in Mouse [ChEMBL: CHEMBL5373] [GtoPdb: 57] [UniProtKB: P47936] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
CB1 receptor/Cannabinoid CB1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL218] [GtoPdb: 56] [UniProtKB: P21554]
ChEMBL	Displacement of human recombinant CB1 receptor expressed in HEK293 cell membranes incubated for 90 mins by Cheng-Prusoff equation based analysis	B	5	pKi	>10000	nM	Ki	Medchemcomm (2014) 5: 1571-1576
ChEMBL	Displacement of [3H]-CP-55940 from human CB1 receptor expressed in CHO cell membranes after 1 hr by beta-counting analysis	B	6	pKi	>1000	nM	Ki	Eur J Med Chem (2019) 165: 347-362 [PMID:30583970]
ChEMBL	Binding affinity to cannabinoid CB1 receptor	B	6.17	pKi	677	nM	Ki	J Med Chem (2010) 53: 4332-4353 [PMID:20218623]
ChEMBL	Displacement of [3H]CP 55940 from human CB1 receptor in cell free system	B	6.17	pKi	677	nM	Ki	Eur J Med Chem (2011) 46: 547-555 [PMID:21183257]
ChEMBL	Binding affinity to CB1 receptor	B	6.17	pKi	677	nM	Ki	Bioorg Med Chem (2011) 19: 5698-5707 [PMID:21872477]
ChEMBL	Displacement of [3H]CP55,940 from human recombinant CB1 receptor expressed in HEK293 cell membranes after 90 mins	B	6.17	pKi	677	nM	Ki	Eur J Med Chem (2014) 74: 524-532 [PMID:24518874]
ChEMBL	Displacement of [3H]CP-55,940 from human recombinant CB1R expressed in HEK-293 cells after 90 mins by liquid scintillation counting	B	6.17	pKi	677	nM	Ki	J Med Chem (2014) 57: 8777-8791 [PMID:25272206]
ChEMBL	Displacement of [3H]CP55,940 from human recombinant CB1 receptor expressed in HEK293 cell membranes after 90 mins	B	6.17	pKi	677	nM	Ki	Eur J Med Chem (2015) 90: 526-536 [PMID:25486424]
ChEMBL	Displacement of [3H]-CP-55940 from recombinant human CB1 receptor overexpressed in HEK293 cell membranes after 90 mins	B	6.17	pKi	677	nM	Ki	Eur J Med Chem (2015) 97: 10-18 [PMID:25935384]
ChEMBL	Binding affinity to CB1 receptor	B	6.17	pKi	677	nM	Ki	J Med Chem (2009) 52: 369-378 [PMID:19143566]
ChEMBL	Displacement of [3H]CP-55940 from human recombinant CB1 receptor expressed in HEK293 cells	B	6.17	pKi	677	nM	Ki	J Med Chem (2009) 52: 3644-3651 [PMID:19435366]
ChEMBL	Displacement of [3H]CP55940 from recombinant human CB1 receptor expressed in human HEK293 cell membrane after 90 mins	B	6.93	pKi	116.8	nM	Ki	Eur J Med Chem (2012) 52: 284-294 [PMID:22483967]
ChEMBL	Displacement of [3H]CP-55940 from human recombinant CB1 receptor	B	9.1	pKi	0.8	nM	Ki	J Med Chem (2009) 52: 369-378 [PMID:19143566]
ChEMBL	Displacement of human recombinant CB1 receptor expressed in HEK293 cell membranes incubated for 90 mins by radioligand binding assay	B	5	pIC50	>10000	nM	IC50	Medchemcomm (2014) 5: 1571-1576
CB1 receptor/Cannabinoid CB1 receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3037] [GtoPdb: 56] [UniProtKB: P47746]
ChEMBL	Displacement of [3H]CP-55940 from CB1 receptor in DBA/J2 mouse brain	B	6.34	pKi	458	nM	Ki	J Med Chem (2006) 49: 5947-5957 [PMID:17004710]
CB1 receptor/Cannabinoid CB1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3571] [GtoPdb: 56] [UniProtKB: P20272]
ChEMBL	Displacement of [3H]CP-55940 from CB1 receptor in Sprague-Dawley rat brain membranes after 1 hr by liquid scintillation spectrophotometry	B	6.17	pKi	677	nM	Ki	Bioorg Med Chem (2010) 18: 7809-7815 [PMID:20943404]
ChEMBL	Binding affinity to rat CB1 receptor	B	6.17	pKi	677	nM	Ki	J Med Chem (2013) 56: 8224-8256 [PMID:23865723]
ChEMBL	Displacement of [35S]GTP-gamma-S from rat cerebellar CB1 receptor	F	5.7	pEC50	1995.26	nM	EC50	J Med Chem (2006) 49: 554-566 [PMID:16420041]
CB2 receptor/Cannabinoid CB2 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL253] [GtoPdb: 57] [UniProtKB: P34972]
ChEMBL	Displacement of human recombinant CB2 receptor expressed in HEK293 cell membranes incubated for 90 mins by Cheng-Prusoff equation based analysis	B	6.85	pKi	140	nM	Ki	Medchemcomm (2014) 5: 1571-1576
ChEMBL	Displacement of [3H]CP-55940 from human CB2 receptor	B	7.69	pKi	20.3	nM	Ki	J Med Chem (2006) 49: 70-79 [PMID:16392793]
ChEMBL	Displacement of [3H]-CP-55940 from human CB2 receptor expressed in CHO cell membranes after 1 hr by beta-counting analysis	B	8.1	pKi	8	nM	Ki	Eur J Med Chem (2019) 165: 347-362 [PMID:30583970]
ChEMBL	Binding affinity to CB2 receptor	B	8.47	pKi	3.4	nM	Ki	J Med Chem (2009) 52: 369-378 [PMID:19143566]
ChEMBL	Binding affinity to cannabinoid CB2 receptor	B	8.47	pKi	3.4	nM	Ki	J Med Chem (2010) 53: 4332-4353 [PMID:20218623]
ChEMBL	Displacement of [3H]CP-55940 from human CB2 receptor expressed in CHO cells after 1 hr by liquid scintillation spectrophotometry	B	8.47	pKi	3.4	nM	Ki	Bioorg Med Chem (2010) 18: 7809-7815 [PMID:20943404]
ChEMBL	Displacement of [3H]CP 55940 from human CB2 receptor in cell free system	B	8.47	pKi	3.4	nM	Ki	Eur J Med Chem (2011) 46: 547-555 [PMID:21183257]
ChEMBL	Binding affinity to CB2 receptor	B	8.47	pKi	3.4	nM	Ki	Bioorg Med Chem (2011) 19: 5698-5707 [PMID:21872477]
ChEMBL	Binding affinity to human CB2 receptor	B	8.47	pKi	3.4	nM	Ki	J Med Chem (2013) 56: 8224-8256 [PMID:23865723]
ChEMBL	Displacement of [3H]-CP55940 from human CB2 receptor expressed in HEK293 cell membranes after 1 hr by liquid scintillation spectrometric method	B	8.47	pKi	3.4	nM	Ki	J Med Chem (2016) 59: 6753-6771 [PMID:27309150]
GtoPdb	-	-	8.5	pKi	3.16	nM	Ki	Bioorg Med Chem (1999) 7: 2905-14 [PMID:10658595]; Expert Opin Investig Drugs (2000) 9: 1553-71 [PMID:11060760]
ChEMBL	Displacement of [3H]-CP-55940 from recombinant human CB2 receptor overexpressed in HEK293 cell membranes after 90 mins	B	8.52	pKi	3	nM	Ki	Eur J Med Chem (2015) 97: 10-18 [PMID:25935384]
ChEMBL	Displacement of [3H]CP-55,940 from human recombinant CB2R expressed in HEK-293 cells after 90 mins by liquid scintillation counting	B	8.52	pKi	3	nM	Ki	J Med Chem (2014) 57: 8777-8791 [PMID:25272206]
ChEMBL	Displacement of [3H]CP55,940 from human recombinant CB2 receptor expressed in HEK293 cell membranes after 90 mins	B	8.52	pKi	3	nM	Ki	Eur J Med Chem (2015) 90: 526-536 [PMID:25486424]
ChEMBL	Displacement of [3H]CP-55940 from human recombinant CB2 receptor expressed in HEK293 cells	B	8.52	pKi	3	nM	Ki	J Med Chem (2009) 52: 3644-3651 [PMID:19435366]
ChEMBL	Displacement of [3H]CP55,940 from human recombinant CB2 receptor expressed in HEK293 cell membranes after 90 mins	B	8.52	pKi	3	nM	Ki	Eur J Med Chem (2014) 74: 524-532 [PMID:24518874]
ChEMBL	Displacement of [3H]CP55940 from recombinant human CB2 receptor expressed in human HEK293 cell membrane after 90 mins	B	8.74	pKi	1.82	nM	Ki	Eur J Med Chem (2012) 52: 284-294 [PMID:22483967]
ChEMBL	Displacement of [3H]CP-55940 from human recombinant CB2 receptor	B	10.64	pKi	0.02	nM	Ki	J Med Chem (2009) 52: 369-378 [PMID:19143566]
ChEMBL	Displacement of human recombinant CB2 receptor expressed in HEK293 cell membranes incubated for 90 mins by radioligand binding assay	B	6.26	pIC50	550	nM	IC50	Medchemcomm (2014) 5: 1571-1576
ChEMBL	Agonist activity at human CB2 receptor expressed in HEK293 cell membranes assessed as inhibition of forskolin-stimulated cAMP accumulation after 60 mins by HTRF assay	F	7.09	pIC50	81.8	nM	IC50	J Med Chem (2016) 59: 6753-6771 [PMID:27309150]
ChEMBL	Agonist activity at human CB2 receptor expressed in CHO-K1 cells assessed as inhibition of NKH-477 induced cAMP production incubated for 30 mins by luminescence based analysis	F	3.77	pEC50	168600	nM	EC50	Eur J Med Chem (2023) 248: 115109-115109 [PMID:36657299]
ChEMBL	Agonist activity at recombinant human CB2R expressed in CHOK1 cells assessed as inhibition of NKH477-stimulated intracellular cAMP levels after 30 mins by chemiluminescent detection based cAMP Hunter assay	F	6.41	pEC50	391.1	nM	EC50	J Med Chem (2020) 63: 7369-7391 [PMID:32515588]
ChEMBL	Agonist activity at human CB2 receptor overexpressed in CHO-K1 cells assessed as cAMP level incubated for 30 mins by luminescence based assay	F	6.77	pEC50	168.6	nM	EC50	J Med Chem (2023) 66: 235-250 [PMID:36542836]
ChEMBL	Effect on [35S]GTP-gamma-S binding to human CB2 receptor	F	6.84	pEC50	145.6	nM	EC50	J Med Chem (2006) 49: 70-79 [PMID:16392793]
ChEMBL	Agonist activity at human CB2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding after 1.5 hrs by liquid scintillation spectrophotometry	F	8.4	pEC50	4	nM	EC50	Bioorg Med Chem (2010) 18: 7809-7815 [PMID:20943404]
CB2 receptor/Cannabinoid CB2 receptor in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5373] [GtoPdb: 57] [UniProtKB: P47936]
ChEMBL	Displacement of [3H]CP-55940 from CB2 receptor in DBA/J2 mouse spleen	B	7.19	pKi	65	nM	Ki	J Med Chem (2006) 49: 5947-5957 [PMID:17004710]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]