ChEMBL ligand: CHEMBL673 (Eutonyl, Eutron, Pargilina, Pargyline)
aldehyde dehydrogenase 2 family member/Aldehyde dehydrogenase in Rat [ChEMBL: CHEMBL2812] [GtoPdb: 2595] [UniProtKB: P11884] There should be some charts here, you may need to enable JavaScript!
ABCG2/ATP-binding cassette sub-family G member 2 in Human [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase A in Human [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397] Monoamine oxidase A in Rat [ChEMBL: CHEMBL3358] [UniProtKB: P21396] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase B in Human [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338] Monoamine oxidase B in Rat [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643] There should be some charts here, you may need to enable JavaScript!
Pyrroline-5-carboxylate reductase 1, mitochondrial in Human [ChEMBL: CHEMBL4296002] [UniProtKB: P32322] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
aldehyde dehydrogenase 2 family member/Aldehyde dehydrogenase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2812] [GtoPdb: 2595] [UniProtKB: P11884]
ChEMBL	Inhibition of Sprague-Dawley rat mitochondrial aldehyde dehydrogenase	B	5.25	pIC50	5600	nM	IC50	J Nat Prod (1997) 60: 1180-1182
ABCG2/ATP-binding cassette sub-family G member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5393] [GtoPdb: 792] [UniProtKB: Q9UNQ0]
ChEMBL	Inhibition of BCRP (unknown origin) transfected in HEK293/R2 cells assessed as reversal of topotecan resistance after 5 days by MTS/PMS assay	B	6	pEC50	>1000	nM	EC50	J Med Chem (2021) 64: 14311-14331 [PMID:34606270]
Monoamine oxidase A in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1951] [GtoPdb: 2489] [UniProtKB: P21397]
ChEMBL	Binding affinity to MAO-A (unknown origin) assessed as inhibition constant	B	4.89	pKi	13000	nM	Ki	J Med Chem (2023) 66: 4275-4293 [PMID:37014989]
ChEMBL	Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI insect cells using kynuramine as substrate after 20 mins by spectrophotometric analysis	B	4.7	pIC50	>20000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 839-843 [PMID:30686752]
ChEMBL	Inhibition of recombinant human MAO-A using kynuramine as substrate by fluorescence spectroscopy	B	4.89	pIC50	13000	nM	IC50	Eur J Med Chem (2017) 135: 196-203 [PMID:28456030]
ChEMBL	Inhibition of recombinant human MAO-A using kynuramine as substrate after 20 mins	B	4.89	pIC50	13000	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4599-4605 [PMID:27578245]
ChEMBL	Inhibition of human recombinant monoamine oxidase-A assessed as kynuramine conversion to 6-hydroxyquinoline after 20 mins by fluorescence spectrophotometric analysis	B	4.89	pIC50	12970	nM	IC50	Bioorg Med Chem Lett (2013) 23: 1269-1273 [PMID:23374869]
ChEMBL	Inhibition of human recombinant MAOA expressed in microsomes of baculovirus-infected insect cell using kynuramine as substrate preincubated for 20 mins followed by protein addition measured after 30 mins by fluorescence assay	B	4.96	pIC50	10900	nM	IC50	J Med Chem (2016) 59: 6791-6806 [PMID:27347731]
ChEMBL	Inhibition of human recombinant MAO-A using pargyline as incubated for 20 mins measuring increase in emission signal at 310 nm multimode plate reader assay	B	5.34	pIC50	4600	nM	IC50	Bioorg Med Chem (2021) 42: 116255-116255 [PMID:34119696]
ChEMBL	Inhibition of human recombinant MAO-A using kynuramine substrate by spectrophotometric assay	B	5.39	pIC50	4100	nM	IC50	J Med Chem (2015) 58: 5561-5578 [PMID:26107513]
ChEMBL	Inhibition of recombinant human microsomal MAOA expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by amplex red reagent-based horseradish peroxidase-coupled fluorometric assay	B	5.4	pIC50	3967.9	nM	IC50	J Med Chem (2020) 63: 1361-1387 [PMID:31917923]
ChEMBL	Inhibition of human MAO-A using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by fluorescence-based Amplex Red MAO assay	B	5.45	pIC50	3520	nM	IC50	Bioorg Med Chem (2017) 25: 5917-5928 [PMID:28988627]
ChEMBL	Inhibition of MAOA (unknown origin) using kynuramine as substrate after 1 hr by fluorescence assay	B	5.47	pIC50	3390	nM	IC50	J Med Chem (2013) 56: 7334-7342 [PMID:24007511]
ChEMBL	Inhibition of human recombinant microsomal MAO-A expressed in baculovirus-infected insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured for 20 mins by amplex red assay	B	5.47	pIC50	3368	nM	IC50	Bioorg Med Chem (2017) 25: 633-645 [PMID:27908752]
ChEMBL	Inhibition of recombinant human MAO-A expressed in baculovirus infected BTI insect cells preincubated for 10 mins followed by addition of kynuramine as substrate	B	5.7	pIC50	>2000	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4714-4719 [PMID:27575476]
ChEMBL	Inhibition of human recombinant MAOA using kynuramine as substrate preincubated for 30 mins followed by substrate addition	B	5.7	pIC50	>2000	nM	IC50	Bioorg Med Chem Lett (2018) 28: 584-588 [PMID:29395970]
ChEMBL	Inhibition of MAOA (unknown origin) using kynuramine as substrate preincubated for 30 mins followed by substrate addition	B	5.88	pIC50	1310	nM	IC50	Medchemcomm (2018) 9: 1871-1881 [PMID:30568755]
ChEMBL	Inhibition of recombinant human MAO-A assessed as reduction in H2O2 production using p-tyramine as substrate preincubated for 15 mins followed by substrate addition by Amplex red reagent based fluorescence assay	B	5.96	pIC50	1100	nM	IC50	Medchemcomm (2017) 8: 1459-1467 [PMID:30108857]
Monoamine oxidase A in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3358] [UniProtKB: P21396]
ChEMBL	Inhibition of MAO-A in rat liver homogenate after 60 mins by Lineweaver-Burke plot	B	5.07	pKi	8500	nM	Ki	Bioorg Med Chem (2009) 17: 675-689 [PMID:19091581]
Monoamine oxidase B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338]
ChEMBL	Binding affinity to human recombinant microsomal MAO-B by ITC	B	6.56	pKd	275.4	nM	Kd	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition constant against human recombinant Monoamine oxidase-B	B	5.74	pKi	1800	nM	Ki	Bioorg Med Chem Lett (2005) 15: 4438-4446 [PMID:16137882]
ChEMBL	Binding affinity to MAO-B (unknown origin) assessed as inhibition constant	B	6.3	pKi	500	nM	Ki	J Med Chem (2023) 66: 4275-4293 [PMID:37014989]
ChEMBL	Inhibition of human recombinant soluble MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay	B	7.31	pKi	49.5	nM	Ki	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition of human recombinant microsomal MAO-B expressed in Pichia pastoris incubated for 30 mins prior to substrate addition measured after 60 mins by MAO-Glo assay	B	7.69	pKi	20.3	nM	Ki	Bioorg Med Chem (2015) 23: 770-778 [PMID:25600407]
ChEMBL	Inhibition of human recombinant MAOB using kynuramine as substrate incubated for 10 mins by UPLC-ESI-MS/MS analysis	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem (2019) 27: 2027-2040 [PMID:30975503]
ChEMBL	Inhibition of human recombinant MAOB expressed in microsomes of baculovirus-infected insect cell using kynuramine as substrate preincubated for 20 mins followed by protein addition measured after 30 mins by fluorescence assay	B	5.57	pIC50	2690	nM	IC50	J Med Chem (2016) 59: 6791-6806 [PMID:27347731]
ChEMBL	Inhibition of MAOB	B	5.59	pIC50	2600	nM	IC50	Eur J Med Chem (2009) 44: 3584-3590 [PMID:19321235]
ChEMBL	Inhibition of human recombinant MAOB using kynuramine substrate incubated for 20 mins by fluorescence spectrophotometry	B	6.11	pIC50	782	nM	IC50	Bioorg Med Chem Lett (2022) 67: 128746-128746 [PMID:35447344]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells assessed as reduction in formation of 4-quinolinol using kynuramine as substrate preincubated with substrate for 10 mins followed by enzyme addition and measured after 20 mins by fluorescence based microplate reader assay	B	6.66	pIC50	220	nM	IC50	Bioorg Med Chem (2020) 28: 115156-115156 [PMID:31740200]
ChEMBL	Inhibition of human recombinant MAO-B using kynuramine as substrate incubated for 20 mins measuring increase in emission signal at 400 nm multimode plate reader assay	B	6.66	pIC50	220	nM	IC50	Bioorg Med Chem (2021) 42: 116255-116255 [PMID:34119696]
ChEMBL	Inhibition of human MAO-B using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by fluorescence-based Amplex Red MAO assay	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem (2017) 25: 5917-5928 [PMID:28988627]
ChEMBL	Inhibition of human MAO-B using p-tyramine as substrate assessed as production of H2O2 from p-tyramine incubated for 15 mins by Amplex Red MAO assay	B	6.7	pIC50	198.5	nM	IC50	Medchemcomm (2015) 6: 1293-1302
ChEMBL	Inhibition of recombinant human microsomal MAOB expressed in baculovirus infected BTI insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition and measured over 20 mins by amplex red reagent-based horseradish peroxidase-coupled fluorometric assay	B	6.71	pIC50	195.5	nM	IC50	J Med Chem (2020) 63: 1361-1387 [PMID:31917923]
ChEMBL	Inhibition of human recombinant microsomal MAO-B expressed in baculovirus-infected insect cells using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured for 20 mins by amplex red assay	B	6.71	pIC50	195	nM	IC50	Bioorg Med Chem (2017) 25: 633-645 [PMID:27908752]
ChEMBL	Inhibition of recombinant human MAO-B using p-tyramine as substrate preincubated for 15 mins followed by substrate addition measured after 20 mins by amplex red reagent based fluorescence assay	B	6.71	pIC50	194	nM	IC50	Eur J Med Chem (2017) 138: 715-728 [PMID:28728104]
ChEMBL	Inhibition of human recombinant MAO-B using p-tyramine as substrate assessed as H2O2 production after 15 mins by fluorescence assay	B	6.72	pIC50	192	nM	IC50	Bioorg Med Chem Lett (2015) 25: 508-513 [PMID:25542589]
ChEMBL	Inhibition of recombinant human MAO-B using p-benzylamine substrate preincubated for 15 mins before substrate addition measured after 20 mins by fluorescence assay	B	6.73	pIC50	188	nM	IC50	Eur J Med Chem (2013) 62: 745-753 [PMID:23454517]
ChEMBL	Inhibition of human recombinant MAO-B using benzylamine as substrate incubated for 15 mins prior to substrate addition measured after 20 mins by fluorescence plate reader analysis	B	6.73	pIC50	188	nM	IC50	Bioorg Med Chem (2015) 23: 3722-3729 [PMID:25934229]
ChEMBL	Inhibition of recombinant human MAOB using benzylamine as substrate preincubated for 15 mins followed by substrate addition measured after 15 mins by Amplex red reagent based fluorescence assay	B	6.73	pIC50	188	nM	IC50	Eur J Med Chem (2018) 143: 33-47 [PMID:29172081]
ChEMBL	Inhibition of human recombinant MAO-B using benzylamine as substrate preincubated for 15 mins under dark condition followed by substrate addition measured after 20 mins by fluorescence assay	B	6.73	pIC50	188	nM	IC50	Bioorg Med Chem (2013) 21: 7406-7417 [PMID:24128814]
ChEMBL	Inhibition of human recombinant microsomal MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as reduction in production of H202 using p-tyramine as substrate incubated for 30 mins followed by substrate addition by horse radish peroxidase/Amplex red reagent based fluorescence assay	B	6.82	pIC50	150	nM	IC50	Eur J Med Chem (2019) 177: 221-234 [PMID:31151057]
ChEMBL	Inhibition of human recombinant MAO-B using kynuramine substrate by spectrophotometric assay	B	6.89	pIC50	130	nM	IC50	J Med Chem (2015) 58: 5561-5578 [PMID:26107513]
ChEMBL	Inhibition of MAOB (unknown origin) using kynuramine as substrate after 1 hr by fluorescence assay	B	6.89	pIC50	130	nM	IC50	J Med Chem (2013) 56: 7334-7342 [PMID:24007511]
ChEMBL	Inhibition of human recombinant MAOB using benzylamine as substrate preincubated for 30 mins followed by substrate addition	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2018) 28: 584-588 [PMID:29395970]
ChEMBL	Inhibition of recombinant human MAO-B using p-tyramine as substrate preincubated with enzyme for 15 mins followed incubation with substrate for 20 mins by Amplex red reagent based fluorescence based analysis	B	6.92	pIC50	120	nM	IC50	RSC Med Chem (2020) 11: 225-233 [PMID:33479629]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI insect cells using kynuramine as substrate incubated for 10 mins followed by substrate addition by fluorimetric analysis	B	6.97	pIC50	107.3	nM	IC50	Bioorg Med Chem (2020) 28: 115550-115550 [PMID:32503694]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells preincubated for 10 mins followed by addition of benzylamine as substrate	B	6.99	pIC50	103	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4714-4719 [PMID:27575476]
ChEMBL	Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI insect cells using benzylamine as substrate after 30 mins by spectrophotometric analysis	B	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2019) 29: 839-843 [PMID:30686752]
ChEMBL	Inhibition of MAOB (unknown origin) using benzylamine as substrate preincubated for 30 mins followed by substrate addition	B	7.04	pIC50	91	nM	IC50	Medchemcomm (2018) 9: 1871-1881 [PMID:30568755]
ChEMBL	Inhibition of recombinant human MAO-B expressed in baculovirus infected BTI insect cells incubated for 15 mins with substrate p-Tyramine by fluorescence assay	B	7.07	pIC50	85.8	nM	IC50	Eur J Med Chem (2019) 180: 367-382 [PMID:31325784]
ChEMBL	Inhibition of human MAO-B preincubated for 5 mins followed by addition of Kynuramine substrate and measured after 30 mins by plate reader method	B	7.1	pIC50	80	nM	IC50	Eur J Med Chem (2021) 211: 113112-113112 [PMID:33360800]
ChEMBL	Inhibition of recombinant human MAO-B assessed as reduction in H2O2 production using p-tyramine as substrate preincubated for 15 mins followed by substrate addition by Amplex red reagent based fluorescence assay	B	7.12	pIC50	75.2	nM	IC50	Medchemcomm (2017) 8: 1459-1467 [PMID:30108857]
ChEMBL	Inhibition of human MAO-B using p-tyramine as substrate by fluorimetric analysis	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2022) 237: 114410-114410 [PMID:35525212]
ChEMBL	Inhibition of MAO-B (unknown origin)	B	8.09	pIC50	8.2	nM	IC50	Eur J Med Chem (2017) 138: 715-728 [PMID:28728104]
Monoamine oxidase B in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643]
ChEMBL	Inhibition of MAO-B in rat liver homogenate after 60 mins by Lineweaver-Burke plot	B	5.47	pKi	3360	nM	Ki	Bioorg Med Chem (2009) 17: 675-689 [PMID:19091581]
ChEMBL	Inhibition of MAO-B in rat liver homogenate after 60 mins by residual activity plot	B	5.41	pIC50	3850	nM	IC50	Bioorg Med Chem (2009) 17: 675-689 [PMID:19091581]
ChEMBL	Inhibition of MAOB in rat liver homogenates preincubated for 60 mins	B	5.41	pIC50	3850	nM	IC50	Bioorg Med Chem Lett (2008) 18: 6362-6368 [PMID:18980841]
ChEMBL	Inhibition of rat liver MAOB after 60 mins pre-incubation	B	5.55	pIC50	2850	nM	IC50	Bioorg Med Chem (2007) 15: 5775-5786 [PMID:17611112]
GtoPdb	-	-	5.74	pIC50	1800	nM	IC50	Biochem Pharmacol (1982) 31: 3555-61 [PMID:6817759]
ChEMBL	Inhibition of Wistar rat brain MAOB using kynuramine as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by fluorescence assay	B	6.66	pIC50	220	nM	IC50	J Nat Prod (2019) 82: 785-791 [PMID:30920216]
Pyrroline-5-carboxylate reductase 1, mitochondrial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4296002] [UniProtKB: P32322]
ChEMBL	Inhibition of 6x-His-tagged and SUMO tagged human PYCR1 expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in NADH oxidation incubated for 1 hr	B	3.7	pIC50	198000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2626-2631 [PMID:31362921]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]