ChEMBL ligand: CHEMBL898 (Diflunisal, Dolobid, Dolobid 500, NSC-756728)
2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in Human [ChEMBL: CHEMBL4105941] [UniProtKB: Q8TDX5] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 1/Carbonic anhydrase I in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 2/Carbonic anhydrase II in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] There should be some charts here, you may need to enable JavaScript!
dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] There should be some charts here, you may need to enable JavaScript!
Organic anion transporter 1/Solute carrier family 22 member 6 in Human [ChEMBL: CHEMBL1641347] [GtoPdb: 1025] [UniProtKB: Q4U2R8] There should be some charts here, you may need to enable JavaScript!
transthyretin/Transthyretin in Human [ChEMBL: CHEMBL3194] [GtoPdb: 2851] [UniProtKB: P02766] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105941] [UniProtKB: Q8TDX5]
ChEMBL	Competitive inhibition of human ACMSD using ACMS substrate by spectrometry	B	5.59	pKi	2560	nM	Ki	J Med Chem (2021) 64: 797-811 [PMID:33369426]
ChEMBL	Inhibition of human ACMSD using ACMS substrate by spectrometry	B	4.87	pIC50	13500	nM	IC50	J Med Chem (2021) 64: 797-811 [PMID:33369426]
carbonic anhydrase 1/Carbonic anhydrase I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL	Inhibition of human carbonic anhydrase 1 esterase activity by noncompetitive Lineweaver-Burke plot	B	5.07	pKi	8450	nM	Ki	Bioorg. Med. Chem. (2008) 16: 9101-9105 [PMID:18819808]
ChEMBL	Inhibition of human carbonic anhydrase 1 esterase activity by spectrophotometry	B	5.47	pIC50	3380	nM	IC50	Bioorg. Med. Chem. (2008) 16: 9101-9105 [PMID:18819808]
carbonic anhydrase 2/Carbonic anhydrase II in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL	Inhibition of human carbonic anhydrase 2 esterase activity by non-competitive Lineweaver-Burke plot	B	5.03	pKi	9370	nM	Ki	Bioorg. Med. Chem. (2008) 16: 9101-9105 [PMID:18819808]
ChEMBL	Inhibition of human carbonic anhydrase 2 esterase activity by spectrophotometry	B	5.57	pIC50	2700	nM	IC50	Bioorg. Med. Chem. (2008) 16: 9101-9105 [PMID:18819808]
dihydrofolate reductase/Dihydrofolate reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374]
ChEMBL	Inhibition of human DHFR in presence of DHF and NADPH by UV-vis spectrometry by Lineweaver-Burk plot analysis	B	4.47	pKi	34000	nM	Ki	J Med Chem (2020) 63: 8314-8324 [PMID:32658475]
GtoPdb	-	-	4.47	pKi	34000	nM	Ki	Journal of Medicinal Chemistry (2020) :
Organic anion transporter 1/Solute carrier family 22 member 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1641347] [GtoPdb: 1025] [UniProtKB: Q4U2R8]
ChEMBL	TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells	F	6.07	pIC50	850	nM	IC50	J. Pharmacol. Exp. Ther. (2000) 295: 10-15 [PMID:10991954]
transthyretin/Transthyretin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3194] [GtoPdb: 2851] [UniProtKB: P02766]
ChEMBL	Stabilization of TTR V30M mutant (unknown origin) assessed as inhibition of protein-mediated amyloid fibril formation	B	5.25	pKd	5600	nM	Kd	J Med Chem (2020) 63: 14228-14242 [PMID:32914975]
ChEMBL	Stabilization of wild type human transthyretin expressed in Escherichia coli BL21(DE3) Star assessed as dissociation constant for TTR-amyloid beta (12 to 28) complex formation at 200 uM at 25 degC pre-incubated with transthyretin before amyloid beta (12 to 28) addition by ITC assay (Rvb = 3 +/- 0.2 microM)	B	5.57	pKd	2700	nM	Kd	J Med Chem (2020) 63: 3205-3214 [PMID:32124607]
ChEMBL	Binding affinity to TTR V30M mutant (unknown origin) by isothermal titration calorimetry	B	5.89	pKd	1300	nM	Kd	J Med Chem (2021) 64: 14344-14357 [PMID:34547896]
ChEMBL	Binding affinity to TTR (unknown origin) by isothermal titration calorimetric analysis	B	6.24	pKd	580	nM	Kd	J. Med. Chem. (2015) 58: 6507-6515 [PMID:26214366]
ChEMBL	Binding affinity to transthyretin (unknown origin) by ITC method	B	6.39	pKd	407	nM	Kd	J Med Chem (2018) 61: 7862-7876 [PMID:30133284]
ChEMBL	Binding affinity to wild type TTR (unknown origin) expressed in Escherichia coli BL21/DE3 by Circular dichroism spectroscopy	B	7.12	pKd	75	nM	Kd	J Med Chem (2020) 63: 14228-14242 [PMID:32914975]
ChEMBL	Binding affinity to TTR in human plasma assessed as protein stabilization preincubated for 1 hr followed by urea-mediated denaturation by Western blot analysis	B	4.6	pIC50	25000	nM	IC50	J. Med. Chem. (2015) 58: 6507-6515 [PMID:26214366]
ChEMBL	Inhibition of ANS binding to TTR V30M mutant (unknown origin) expressed in Escherichia coli assessed as BC50 for ANS binding to TTR at 5 uM in presence of 150 mM Na+ by fluorescence-based assay (Rvb = 3 uM)	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2019) 62: 2076-2082 [PMID:30688456]
ChEMBL	Inhibition of acid-mediated aggregation of TTR V30M mutant (unknown origin) expressed in Escherichia coli pretreated for 30 mins at pH 7 followed by protein dilution in acetate buffer and further incubated for 96 hrs at pH 4.6 by thioflavin-T fluorescence assay	B	5.18	pIC50	6600	nM	IC50	Bioorg Med Chem (2021) 44: 116292-116292 [PMID:34225167]
ChEMBL	Inhibition of transthyretin V30M mutant (unknown origin) expressed in Escherichia coli assessed as inhibition of sodium acetate-mediated aggregation preincubated for 30 mins followed by sodium acetate addition measured after 96 hrs by thioflavin T assay	B	5.2	pIC50	6300	nM	IC50	J. Med. Chem. (2014) 57: 1090-1096 [PMID:24422526]
ChEMBL	Stabilization of TTR V3OM mutant (unknown origin) assessed as acid-mediated protein aggregation inhibition ratio incubated for 1 week by absorbance method	B	5.46	pIC50	3500	nM	IC50	J Med Chem (2021) 64: 14344-14357 [PMID:34547896]
ChEMBL	Inhibition of TTR V30M mutant (unknown origin) expressed in Escherichia coli assessed as inhibition of amyloid fibril formation by fluorescence assay	B	5.25	pEC50	5600	nM	EC50	J. Med. Chem. (2014) 57: 8928-8935 [PMID:25314129]

ChEMBL data shown on this page come from version 31:

Gaulton A, Hersey A, Nowotka M, Bento AP, Chambers J, Mendez D, Mutowo P, Atkinson F, Bellis LJ, Cibrián-Uhalte E, Davies M, Dedman N, Karlsson A, Magariños MP, Overington JP, Papadatos G, Smit I, Leach AR. (2017) 'The ChEMBL database in 2017.' Nucleic Acids Res., 45(D1). DOI: 10.1093/nar/gkw1074. [PMCID:5210557]