ChEMBL ligand: CHEMBL1231795
DNA methyltransferase 1/DNA (cytosine-5)-methyltransferase 1 in Human [ChEMBL: CHEMBL1993] [GtoPdb: 2605] [UniProtKB: P26358] There should be some charts here, you may need to enable JavaScript!
euchromatic histone lysine methyltransferase 2/Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 in Human [ChEMBL: CHEMBL6032] [GtoPdb: 2652] [UniProtKB: Q96KQ7] There should be some charts here, you may need to enable JavaScript!
euchromatic histone lysine methyltransferase 1/Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 in Human [ChEMBL: CHEMBL6031] [GtoPdb: 2651] [UniProtKB: Q9H9B1] There should be some charts here, you may need to enable JavaScript!
lysine demethylase 1A/Lysine-specific histone demethylase 1 in Human [ChEMBL: CHEMBL6136] [GtoPdb: 2669] [UniProtKB: O60341] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
spindlin 1/Spindlin-1 in Human [ChEMBL: CHEMBL4523509] [GtoPdb: 3274] [UniProtKB: Q9Y657] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
DNA methyltransferase 1/DNA (cytosine-5)-methyltransferase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1993] [GtoPdb: 2605] [UniProtKB: P26358]
ChEMBL	Inhibition of human DNMT1 using polydeoxyinosine polydeoxycytosine DNA as substrate after 15 mins in presence of SAM by TR-FRET assay	B	5.7	pIC50	2010	nM	IC50	J Med Chem (2018) 61: 6518-6545 [PMID:29953809]
ChEMBL	Inhibition of human DNMT1 using polydeoxyinosine polydeoxycytosine DNA as substrate after 15 mins in presence of SAM by TR-FRET assay	B	5.7	pIC50	2010	nM	IC50	J Med Chem (2018) 61: 6546-6573 [PMID:29890830]
ChEMBL	Inhibition of DNMT1 (unknown origin) by radioactive methyl transfer assay	B	5.89	pIC50	1287	nM	IC50	J Med Chem (2018) 61: 6518-6545 [PMID:29953809]
ChEMBL	Inhibition of DNMT1 (unknown origin)	B	5.89	pIC50	1287	nM	IC50	J Med Chem (2018) 61: 6546-6573 [PMID:29890830]
euchromatic histone lysine methyltransferase 2/Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6032] [GtoPdb: 2652] [UniProtKB: Q96KQ7]
ChEMBL	Binding affinity to human His-tagged G9a expressed in Escherichia coli Rosetta (DE3) after 30 mins by MST binding assay	B	5.85	pKd	1420	nM	Kd	J Med Chem (2019) 62: 2666-2689 [PMID:30753076]
ChEMBL	Inhibition of G9a (unknown origin) assessed as reduction in substrate methylation using histone H3 and SAM as substrate measured after 15 to 60 mins by microfluidic capillary electrophoresis analysis	B	7.74	pKi	18	nM	Ki	Eur J Med Chem (2022) 237: 114410-114410 [PMID:35525212]
ChEMBL	Competitive inhibition of G9a by fluorescence polarization assay in presence of fluorescein-labeled H3 peptide	B	8.43	pKi	3.7	nM	Ki	Eur J Med Chem (2012) 56: 179-194 [PMID:22975593]
ChEMBL	Competitive inhibition of G9a (unknown origin) by Morrison plot analysis in presence of histone H3 (1 to 25 residues)	B	8.43	pKi	3.7	nM	Ki	Bioorg Med Chem (2016) 24: 6102-6108 [PMID:27720557]
GtoPdb	-	-	8.52	pKi	3	nM	Ki	Nat Chem Biol (2011) 7: 566-74 [PMID:21743462]
ChEMBL	Inhibition of G9a (unknown origin) using biotinylated-histone H3 (1 to 21 residues)/S-adenosyl-methionine as substrate/methyl donor after 3 hrs by AlphaScreen assay	B	6.21	pIC50	610	nM	IC50	Bioorg Med Chem (2016) 24: 6102-6108 [PMID:27720557]
ChEMBL	Inhibition of G9a in p53-deficient human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of G9a in human HCT116 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	6.68	pIC50	210	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of G9a in human IMR90 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	6.92	pIC50	120	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of lysine methyltransferase G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 cellular level by immunofluorescence in-cell Western assay	B	7.09	pIC50	81	nM	IC50	J Med Chem (2013) 56: 8931-8942 [PMID:24102134]
ChEMBL	Inhibition of G9a in human MDA-MB-231 cells after 48 hrs by clonogenic assay	B	7.09	pIC50	81	nM	IC50	Eur J Med Chem (2012) 56: 179-194 [PMID:22975593]
ChEMBL	Inhibition of G9a in human MCF7 cells after 48 hrs by clonogenic assay	B	7.09	pIC50	81	nM	IC50	Eur J Med Chem (2012) 56: 179-194 [PMID:22975593]
ChEMBL	Inhibition of G9a in human MDA-MB-231 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	7.09	pIC50	81	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of G9a in human MCF7 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	7.15	pIC50	70	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of G9a in human PC3 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	7.23	pIC50	59	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of human G9a using biotinylated-H3K9 peptide as substrate after 1 hr in presence of SAM by TR-FRET assay	B	7.26	pIC50	55	nM	IC50	J Med Chem (2018) 61: 6546-6573 [PMID:29890830]
ChEMBL	Inhibition of human G9a using biotinylated-H3K9 peptide as substrate after 1 hr in presence of SAM by TR-FRET assay	B	7.26	pIC50	55	nM	IC50	J Med Chem (2018) 61: 6518-6545 [PMID:29953809]
ChEMBL	Inhibition of G9a in human 22Rv1 cells assessed as reduction of H3K9me2 after 48 hrs by In-Cell Western assay	B	7.32	pIC50	48	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of N-terminal GST-tagged human recombinant G9a (785 to 1210 residues) expressed in baculovirus infected Sf9 cells incubated for 1 hr by AlphaLISA analysis	B	7.45	pIC50	35.3	nM	IC50	J Med Chem (2023) 66: 4059-4085 [PMID:36882960]
ChEMBL	Inhibition of human G9a using core histone H3 as substrate incubated for 1 hr in presence of [3H]-SAM by filter binding method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2019) 184: 111755-111755 [PMID:31627059]
ChEMBL	Inhibition of N-terminal GST tagged human recombinant EHMT2 (785 to 1210 residues) expressed in baculovirus infected Sf9 insect cells assessed as reduction in conversion of SAH to AMP using histone H3 as substrate and SAH as cosubstrate incubated for 30 mins by AMP Glo detection assay	B	7.59	pIC50	26	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2516-2524 [PMID:31350126]
ChEMBL	Inhibition of recombinant human G9a using biotinylated-Histone H3 peptide (1 to 21 residues) after 30 mins in presence of SAM by AlphaLISA assay	B	7.6	pIC50	25	nM	IC50	J Med Chem (2019) 62: 2666-2689 [PMID:30753076]
GtoPdb	Measuring conversion of the cofactor SAM to product SAH (S-adenosyl-l-homocysteine).	-	7.82	pIC50	<15	nM	IC50	Nat Chem Biol (2011) 7: 566-74 [PMID:21743462]
ChEMBL	Inhibition of G9a (unknown origin) using H3(1 to 25) as substrate assessed as conversion of SAM to SAH preincubated for 2 mins followed by substrate addition measured for 20 mins by SAHH-coupled assay	B	7.82	pIC50	<15	nM	IC50	Medchemcomm (2012) 3: 135-161
ChEMBL	Competitive inhibition of G9a by fluorescence polarization assay in presence of fluorescein-labeled H3 peptide	B	7.82	pIC50	<15	nM	IC50	Eur J Med Chem (2012) 56: 179-194 [PMID:22975593]
ChEMBL	Inhibition of G9a (unknown origin) using H3(1-20)-cys as substrate by HPLC analysis	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2023) 246: 115028-115028 [PMID:36528996]
ChEMBL	Inhibition G9a (unknown origin)	B	7.82	pIC50	15	nM	IC50	J Med Chem (2019) 62: 2666-2689 [PMID:30753076]
ChEMBL	Inhibition of G9a (unknown origin) using histone H3 (1 to 25 residues) as substrate preincubated for 2 mins followed by substrate addition measured for 20 mins by SAHH-coupled assay	B	7.82	pIC50	<15	nM	IC50	Bioorg Med Chem (2016) 24: 6102-6108 [PMID:27720557]
ChEMBL	Inhibition of EHMT2 (unknown origin)	B	7.82	pIC50	<15	nM	IC50	Bioorg Med Chem (2017) 25: 4579-4594 [PMID:28739157]
ChEMBL	Inhibition of G9a (unknown origin)	B	7.82	pIC50	<15	nM	IC50	J Med Chem (2018) 61: 6546-6573 [PMID:29890830]
ChEMBL	Inhibition of G9a (unknown origin)	B	7.82	pIC50	<15	nM	IC50	Eur J Med Chem (2019) 184: 111755-111755 [PMID:31627059]
ChEMBL	Inhibition G9a (unknown origin)	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2019) 179: 537-546 [PMID:31276898]
ChEMBL	Inhibition of G9a assessed as hydrolysis of S-adenosyl-L-homocysteine after 2 mins by SAHH-coupled fluorescence assay	B	7.92	pIC50	12	nM	IC50	J Med Chem (2011) 54: 6139-6150 [PMID:21780790]
ChEMBL	Inhibition of G9a (unknown origin)	B	7.92	pIC50	12	nM	IC50	J Med Chem (2023) 66: 8086-8102 [PMID:37268593]
ChEMBL	Inhibition of G9a (unknown origin)	B	8.03	pIC50	9.4	nM	IC50	ACS Med Chem Lett (2023) 14: 1531-1536 [PMID:37974951]
ChEMBL	Inhibition of human recombinant EHMT2 G9a using SAM as substrate incubated for 1 hr by microplate luminescence based HMT assay	B	8.43	pIC50	3.75	nM	IC50	J Med Chem (2023) 66: 5685-5702 [PMID:37021456]
ChEMBL	Inhibition of lysine methyltransferase G9a (unknown origin) using [3H]-SAM as substrate after 0.25 hrs by scintillation proximity assay	B	8.6	pIC50	<2.5	nM	IC50	J Med Chem (2013) 56: 8931-8942 [PMID:24102134]
ChEMBL	Inhibition of G9a (unknown origin) using [histone H3 1 to 25 residues] and SAM substrate by scintillation proximity assay	B	8.6	pIC50	2.5	nM	IC50	Medchemcomm (2014) 5: 1821-1828 [PMID:25750706]
euchromatic histone lysine methyltransferase 1/Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6031] [GtoPdb: 2651] [UniProtKB: Q9H9B1]
GtoPdb	Measuring conversion of cofactor SAM to product SAH (S-adenosyl-l-homocysteine).	-	7.72	pIC50	19	nM	IC50	Nat Chem Biol (2011) 7: 566-74 [PMID:21743462]
ChEMBL	Inhibition of GLP (unknown origin)	B	7.72	pIC50	19	nM	IC50	J Med Chem (2023) 66: 8086-8102 [PMID:37268593]
ChEMBL	Inhibition of GLP (unknown origin) assessed as conversion of SAM to SAH by SAHH-coupled assay	B	7.72	pIC50	19	nM	IC50	Medchemcomm (2012) 3: 135-161
ChEMBL	Inhibition of EHMT1 (unknown origin)	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem (2017) 25: 4579-4594 [PMID:28739157]
ChEMBL	Competitive inhibition of GLP by fluorescence polarization assay in presence of fluorescein-labeled H3 peptide	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2012) 56: 179-194 [PMID:22975593]
ChEMBL	Inhibition of GLP (unknown origin)	B	7.72	pIC50	19	nM	IC50	J Med Chem (2019) 62: 2666-2689 [PMID:30753076]
ChEMBL	Inhibition of GLP (unknown origin)	B	7.72	pIC50	19	nM	IC50	J Med Chem (2023) 66: 4059-4085 [PMID:36882960]
ChEMBL	Inhibition of GLP (unknown origin) using H3(1-20)-cys as substrate by HPLC analysis	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2023) 246: 115028-115028 [PMID:36528996]
ChEMBL	Inhibition of N-terminal GST tagged human recombinant EHMT1 (794 to 1294 residues) expressed in baculovirus infected Sf9 insect cells assessed as reduction in conversion of SAH to AMP using histone H3 as substrate and SAH as cosubstrate incubated for 30 mins by AMP Glo detection assay	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem Lett (2019) 29: 2516-2524 [PMID:31350126]
lysine demethylase 1A/Lysine-specific histone demethylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6136] [GtoPdb: 2669] [UniProtKB: O60341]
ChEMBL	Inhibition of human LSD1 (unknown orgin)	B	6.74	pIC50	180	nM	IC50	Eur J Med Chem (2021) 222: 113588-113588 [PMID:34107385]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against asynchronous form of Plasmodium falciparum 3D7 infected in human erythrocytes assessed as parasite growth inhibition after 48 hrs by SYBR green1 staining based flow cytometry	F	7.17	pIC50	67.9	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
ChEMBL	Antiplasmodial activity against asynchronous form of Plasmodium falciparum W2 infected in human erythrocytes assessed as parasite growth inhibition after 48 hrs by SYBR green1 staining based flow cytometry	F	7.41	pIC50	38.5	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum Dd2 infected in human erythrocytes assessed as reduction in [3H]-hypoxanthine incorporation measured after 48 hrs by microbeta liquid scintillation counting method	F	7.77	pIC50	16.8	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
spindlin 1/Spindlin-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523509] [GtoPdb: 3274] [UniProtKB: Q9Y657]
ChEMBL	Inhibition of FL-H3K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 30 mins by fluorescence polarization displacement assay	B	5.13	pIC50	7400	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]