ChEMBL ligand: CHEMBL435 (Apo-hydro, Apresazide, Carozide, Chlorosulthiadil, Copalia-hct, Dafiro-hct, Dihydrochlorurite, Esidrex, Esidrix, Exforge-hct, Hctz, Hidroclorotiazida, Hidrotiazida, Hydrochlorothiaizide, Hydrochlorothiazide, Hydrochlorothiazide intensol, Hydrochlorothiazidum, Hydro-d, Hydrodiuril, Hydrosaluric, Idrotiazide, Imprida-hct, Lotensin hct, Megadiuril, Microzide, Newtolide, NSC-53477, Oretic, Pantemon, Rasilez-hct, Servithiazid, SU-5879, Unipres, Vetidrex, Zide)
AGAP002992-PA in Anopheles gambiae [ChEMBL: CHEMBL3399914] [UniProtKB: Q5TU56] There should be some charts here, you may need to enable JavaScript!
Astrosclerin-3 in Astrosclera willeyana [ChEMBL: CHEMBL1944499] [UniProtKB: A6YCJ1] There should be some charts here, you may need to enable JavaScript!
Carbonate dehydratase in Sulfurihydrogenibium sp. (strain YO3AOP1) [ChEMBL: CHEMBL3745586] [UniProtKB: B2V8E3] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase, alpha family in Thiomicrospira crunogena (strain XCL-2) [ChEMBL: CHEMBL3621035] [UniProtKB: Q31FD6] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 1/Carbonic anhydrase I in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 2/Carbonic anhydrase II in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] There should be some charts here, you may need to enable JavaScript!
Delta carbonic anhydrase in Thalassiosira weissflogii [ChEMBL: CHEMBL3097982] [UniProtKB: Q5U9J1] There should be some charts here, you may need to enable JavaScript!
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
AGAP002992-PA in Anopheles gambiae (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3399914] [UniProtKB: Q5TU56]
ChEMBL	Inhibition of Anopheles gambiae carbonic anhydrase pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	7.5	pKi	31.5	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
Astrosclerin-3 in Astrosclera willeyana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1944499] [UniProtKB: A6YCJ1]
ChEMBL	Inhibition of GST-tagged astrosclera willeyana Astrosclerin-3 expressed in Escherichia coli after 15 mins preincubation by stopped flow CO2 hydration assay	B	6.4	pKi	394	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
Carbonate dehydratase in Sulfurihydrogenibium sp. (strain YO3AOP1) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3745586] [UniProtKB: B2V8E3]
ChEMBL	Inhibition of recombinant Sulfurihydrogenibium yellowstonense YO3AOP1 carbonic anhydrase by stopped flow CO2 hydrase assay	B	8.14	pKi	7.2	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
Carbonic anhydrase, alpha family in Thiomicrospira crunogena (strain XCL-2) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3621035] [UniProtKB: Q31FD6]
ChEMBL	Inhibition of recombinant Thiomicrospira crunogena XCL-2 carbonic anhydrase by stopped flow CO2 hydrase assay	B	6.53	pKi	296	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
carbonic anhydrase 1/Carbonic anhydrase I in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL	Inhibition of human carbonic anhydrase 1 after 15 mins by CO2 hydrase assay at pH 7.5	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2011) 19: 1847-1851 [PMID:21377369]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 at pH 7.5 by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2011) 19: 5023-5030 [PMID:21757360]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 after 15 mins by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 preincubated for 15 mins by stopped-flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	J Med Chem (2012) 55: 10742-10748 [PMID:23181552]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	J Med Chem (2013) 56: 1761-1771 [PMID:23391336]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 preincubated for 15 mins by CO2 hydration stopped-flow assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2013) 21: 1534-1538 [PMID:22883029]
ChEMBL	Inhibition of human carbonic anhydrase-1 by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2014) 22: 2939-2946 [PMID:24792813]
ChEMBL	Inhibition of human recombinant Carbonic anhydrase 1 compound preincubated for 15 mins by stopped flow CO2 hydrase assay method	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2014) 22: 4537-4543 [PMID:25129169]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 526-531 [PMID:25533402]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 1728-1734 [PMID:25773015]
ChEMBL	Inhibition of human carbonic anhydrase 1 pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
ChEMBL	Inhibition of human CA1 incubated for 15 mins prior to testing by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2015) 25: 5485-5489 [PMID:26525863]
ChEMBL	Inhibition of recombinant human carbonic anhydrase-1 by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
ChEMBL	Inhibition of human Carbonic anhydrase1 using CO2 as substrate preincubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2016) 24: 1115-1120 [PMID:26850377]
ChEMBL	Inhibition of human carbonic anhydrase 1 incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1821-1826 [PMID:26920803]
ChEMBL	Inhibition of recombinant human carbonic anhydrase 1 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 sec by Line-Weaver Burk plot analysis	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2017) 27: 490-495 [PMID:28025002]
ChEMBL	Inhibition of human carbonic anhydrase-1 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 secs by stopped-flow assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2017) 25: 3555-3561 [PMID:28511911]
ChEMBL	Inhibition of full length human cytosolic carbonic anhydrase 1 preincubated for 15 mins by stop flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4184-4190 [PMID:27485387]
carbonic anhydrase 2/Carbonic anhydrase II in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL	Inhibition of human carbonic anhydrase 2 after 15 mins by CO2 hydrase assay at pH 7.5	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2011) 19: 1847-1851 [PMID:21377369]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 at pH 7.5 by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2011) 19: 5023-5030 [PMID:21757360]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 after 15 mins by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by stopped-flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	J Med Chem (2012) 55: 10742-10748 [PMID:23181552]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	J Med Chem (2013) 56: 1761-1771 [PMID:23391336]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by CO2 hydration stopped-flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2013) 21: 1534-1538 [PMID:22883029]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2014) 24: 275-279 [PMID:24314394]
ChEMBL	Inhibition of human carbonic anhydrase-2 by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2014) 22: 2939-2946 [PMID:24792813]
ChEMBL	Inhibition of human recombinant Carbonic anhydrase 2 compound preincubated for 15 mins by stopped flow CO2 hydrase assay method	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2014) 22: 4537-4543 [PMID:25129169]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 526-531 [PMID:25533402]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 1728-1734 [PMID:25773015]
ChEMBL	Inhibition of human carbonic anhydrase 2 pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
ChEMBL	Inhibition of human recombinant carbonic anhydrase-2 by stopped flow CO2 hydrase assay method	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2015) 25: 3550-3555 [PMID:26174556]
ChEMBL	Inhibition of human CA2 incubated for 15 mins prior to testing by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2015) 25: 5485-5489 [PMID:26525863]
ChEMBL	Inhibition of recombinant human carbonic anhydrase-2 by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
ChEMBL	Inhibition of human Carbonic anhydrase2 using CO2 as substrate preincubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2016) 24: 1115-1120 [PMID:26850377]
ChEMBL	Inhibition of human carbonic anhydrase 2 preincubated for 15 mins by CO2 hydrase stopped flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1253-1259 [PMID:26832216]
ChEMBL	Inhibition of human carbonic anhydrase 2 incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1821-1826 [PMID:26920803]
ChEMBL	Inhibition of human carbonic anhydrase 2 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 sec by Line-Weaver Burk plot analysis	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2017) 27: 490-495 [PMID:28025002]
ChEMBL	Inhibition of human carbonic anhydrase-2 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 secs by stopped-flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2017) 25: 3555-3561 [PMID:28511911]
ChEMBL	Inhibition of human cytosolic carbonic anhydrase 2 preincubated for 15 mins by stop flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4184-4190 [PMID:27485387]
Delta carbonic anhydrase in Thalassiosira weissflogii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3097982] [UniProtKB: Q5U9J1]
ChEMBL	Inhibition of Thalassiosira weissflogii delta carbonic anhydrase preincubated for 15 mins by stopped flow CO2 hydration assay	B	5.37	pKi	4270	nM	Ki	Bioorg Med Chem Lett (2014) 24: 275-279 [PMID:24314394]
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20]
GtoPdb	Determined by surface plasmon resonance, in the absence of deoxycholic acid in the bile acid binding domain	-	5.1	pKd	8000	nM	Kd	Cell Chem Biol (2025) : [PMID:39999832]
GtoPdb	equilibrium dissociation constant in presence of deoxycholic acid, determined by SPR	-	6.13	pKd	740	nM	Kd	Cell Chem Biol (2025) : [PMID:39999832]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]