ChEMBL ligand: CHEMBL435 (Esidrex, Hydrosaluric, Microzide, Hydrochlorothiazide component of monopril hct, Servithiazid, Hydrochlorothiazide component of lotensin hct, Hydrochlorothiazide component of hydroserpine plus (r-h-h), Hydrochlorothiazide component of maxzide, Hydrochlorothiaizide, Chlorosulthiadil, Hydrochlorothiazide component of esimil, Zide, Pantemon, Hydrochlorothiazide component of irbesartan/ teva, Hydrochlorothiazide component of teveten hct, Esidrix, Hydrochlorothiazide component of lopressor hct, Hydrochlorothiazide component of avalide, Hydrochlorothiazide component of hyzaar, Hydrochlorothiazide component of dutoprol, Hydrochlorothiazide component of viskazide, Hydrochlorothiazide component of micardis hct, Hydrochlorothiazide component of cam-ap-es, Hydrodiuril, Hydrochlorothiazide component of tekturna hct, Hydrochlorothiazide component of ser-ap-es, SU-5879, Hydrochlorothiazide component of oreticyl forte, Hydrochlorothiazide component of moduretic, Hydrochlorothiazide component of ziac, Exforge-hct, Hydrochlorothiazide component of h.r.-50, Hydrochlorothiazide component of hydra-zide, Hydrochlorothiazide component of irbesartan/-bms, Hydrochlorothiazide component of exforge hct, Hydrochlorothiazide component of apresoline-esidrix, Hydrochlorothiazide component of kinzalkomb, Hydrochlorothiazide component of serpasil-esidrix, NSC-53477, Rasilez-hct, Oretic, Hydrochlorothiazide component of ibersartan/ zentiva, Hidroclorotiazida, Hydrochlorothiazide, Dafiro-hct, Hydrochlorothiazide component of benicar hct, Hydrochlorothiazide component of tolucombi, Hydrochlorothiazide component of aldoril, Hydrochlorothiazide component of unipres, Hydrochlorothiazide component of ifirmacombi, Copalia-hct, Hidrotiazida, Hydrochlorothiazide component of vaseretic, Hydrochlorothiazide component of quinaretic, Hydrochlorothiazide component of pritorplus, Unipres, Hydrochlorothiazide component of riprazo, Megadiuril, Hydrochlorothiazide component of hydralazine hydrochloride--reserpine, Hydrochlorothiazide component of sprimeo-hct, Hydrochlorothiazide component of rasitrio, Apo-hydro, Ziac, Carozide, Hydrochlorothiazide intensol, Hydrochlorothiazide component of amturnide, Hydrochlorothiazide component of tribenzor, Hydrochlorothiazide component of uniretic, Hydrochlorothiazide component of prinzide, Hydrochlorothiazide component of hydro-ride, Lotensin hct, Newtolide, Hydrochlorothiazide component of normozide, Hydrochlorothiazide component of kervezide, Hydrochlorothiazide component of diovan hct, Hydrochlorothiazide component of hydro-reserp, Hydrochlorothiazide component of micardisplus, Hydrochlorothiazide component of polycap, Hydro-d, Hydrochlorothiazide component of zestoretic, Dihydrochlorurite, Hydrochlorothiazide component of hydropres, Hydrochlorothiazide component of accuretic, Vetidrex, Imprida-hct, Hydrochlorothiazide component of dyazide, Hydrochlorothiazide component of apresazide, Hydrochlorothiazide component of hydrap-es, Hydrochlorthiazide, Idrotiazide, Hydrochlorothiazide component of ser-a-gen, Hydrochlorothiazide component of aldactazide, Hydrochlorothiazidum, Apresazide)
AGAP002992-PA in Anopheles gambiae [ChEMBL: CHEMBL3399914] [UniProtKB: Q5TU56] There should be some charts here, you may need to enable JavaScript!
Astrosclerin-3 in Astrosclera willeyana [ChEMBL: CHEMBL1944499] [UniProtKB: A6YCJ1] There should be some charts here, you may need to enable JavaScript!
Carbonate dehydratase in Sulfurihydrogenibium sp. (strain YO3AOP1) [ChEMBL: CHEMBL3745586] [UniProtKB: B2V8E3] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 1/Carbonic anhydrase 1 in Human [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915] There should be some charts here, you may need to enable JavaScript!
carbonic anhydrase 2/Carbonic anhydrase 2 in Human [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918] There should be some charts here, you may need to enable JavaScript!
Carbonic anhydrase, alpha family in Thiomicrospira crunogena (strain XCL-2) [ChEMBL: CHEMBL3621035] [UniProtKB: Q31FD6] There should be some charts here, you may need to enable JavaScript!
Delta carbonic anhydrase in Thalassiosira weissflogii [ChEMBL: CHEMBL3097982] [UniProtKB: Q5U9J1] There should be some charts here, you may need to enable JavaScript!
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
AGAP002992-PA in Anopheles gambiae (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3399914] [UniProtKB: Q5TU56]
ChEMBL	Inhibition of Anopheles gambiae carbonic anhydrase pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	7.5	pKi	31.5	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
Astrosclerin-3 in Astrosclera willeyana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1944499] [UniProtKB: A6YCJ1]
ChEMBL	Inhibition of GST-tagged astrosclera willeyana Astrosclerin-3 expressed in Escherichia coli after 15 mins preincubation by stopped flow CO2 hydration assay	B	6.4	pKi	394	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
Carbonate dehydratase in Sulfurihydrogenibium sp. (strain YO3AOP1) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3745586] [UniProtKB: B2V8E3]
ChEMBL	Inhibition of recombinant Sulfurihydrogenibium yellowstonense YO3AOP1 carbonic anhydrase by stopped flow CO2 hydrase assay	B	8.14	pKi	7.2	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
carbonic anhydrase 1/Carbonic anhydrase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL261] [GtoPdb: 2597] [UniProtKB: P00915]
ChEMBL	Inhibition of human carbonic anhydrase 1 after 15 mins by CO2 hydrase assay at pH 7.5	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2011) 19: 1847-1851 [PMID:21377369]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 at pH 7.5 by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2011) 19: 5023-5030 [PMID:21757360]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 after 15 mins by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 preincubated for 15 mins by stopped-flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	J Med Chem (2012) 55: 10742-10748 [PMID:23181552]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	J Med Chem (2013) 56: 1761-1771 [PMID:23391336]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 preincubated for 15 mins by CO2 hydration stopped-flow assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2013) 21: 1534-1538 [PMID:22883029]
ChEMBL	Inhibition of human carbonic anhydrase-1 by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2014) 22: 2939-2946 [PMID:24792813]
ChEMBL	Inhibition of human recombinant Carbonic anhydrase 1 compound preincubated for 15 mins by stopped flow CO2 hydrase assay method	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2014) 22: 4537-4543 [PMID:25129169]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 526-531 [PMID:25533402]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 1 by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 1728-1734 [PMID:25773015]
ChEMBL	Inhibition of human carbonic anhydrase 1 pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
ChEMBL	Inhibition of human CA1 incubated for 15 mins prior to testing by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2015) 25: 5485-5489 [PMID:26525863]
ChEMBL	Inhibition of recombinant human carbonic anhydrase-1 by stopped flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
ChEMBL	Inhibition of human Carbonic anhydrase1 using CO2 as substrate preincubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2016) 24: 1115-1120 [PMID:26850377]
ChEMBL	Inhibition of human carbonic anhydrase 1 incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1821-1826 [PMID:26920803]
ChEMBL	Inhibition of recombinant human carbonic anhydrase 1 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 sec by Line-Weaver Burk plot analysis	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2017) 27: 490-495 [PMID:28025002]
ChEMBL	Inhibition of human carbonic anhydrase-1 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 secs by stopped-flow assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem (2017) 25: 3555-3561 [PMID:28511911]
ChEMBL	Inhibition of full length human cytosolic carbonic anhydrase 1 preincubated for 15 mins by stop flow CO2 hydrase assay	B	6.48	pKi	328	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4184-4190 [PMID:27485387]
carbonic anhydrase 2/Carbonic anhydrase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL205] [GtoPdb: 3092] [UniProtKB: P00918]
ChEMBL	Displacement of dansylamide from human recombinant CA II expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by fluorescence microplate reader	B	5.14	pKd	7190	nM	Kd	J Med Chem (2024) 67: 15862-15872 [PMID:39161321]
ChEMBL	Binding affinity to human recombinant CA II assessed as dissociation constant	B	5.59	pKd	2600	nM	Kd	J Med Chem (2024) 67: 15862-15872 [PMID:39161321]
ChEMBL	Inhibition of human carbonic anhydrase 2 after 15 mins by CO2 hydrase assay at pH 7.5	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2011) 19: 1847-1851 [PMID:21377369]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 at pH 7.5 by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2011) 19: 5023-5030 [PMID:21757360]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 after 15 mins by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2012) 20: 1403-1410 [PMID:22285172]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by stopped-flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	J Med Chem (2012) 55: 10742-10748 [PMID:23181552]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	J Med Chem (2013) 56: 1761-1771 [PMID:23391336]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by CO2 hydration stopped-flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2013) 21: 1534-1538 [PMID:22883029]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 preincubated for 15 mins by stopped flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2014) 24: 275-279 [PMID:24314394]
ChEMBL	Inhibition of human carbonic anhydrase-2 by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2014) 22: 2939-2946 [PMID:24792813]
ChEMBL	Inhibition of human recombinant Carbonic anhydrase 2 compound preincubated for 15 mins by stopped flow CO2 hydrase assay method	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2014) 22: 4537-4543 [PMID:25129169]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 526-531 [PMID:25533402]
ChEMBL	Inhibition of human recombinant carbonic anhydrase 2 by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 1728-1734 [PMID:25773015]
ChEMBL	Inhibition of human carbonic anhydrase 2 pre-incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2015) 23: 2303-2309 [PMID:25882523]
ChEMBL	Inhibition of human recombinant carbonic anhydrase-2 by stopped flow CO2 hydrase assay method	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2015) 25: 3550-3555 [PMID:26174556]
ChEMBL	Inhibition of human CA2 incubated for 15 mins prior to testing by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2015) 25: 5485-5489 [PMID:26525863]
ChEMBL	Inhibition of recombinant human carbonic anhydrase-2 by stopped flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
ChEMBL	Inhibition of human Carbonic anhydrase2 using CO2 as substrate preincubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2016) 24: 1115-1120 [PMID:26850377]
ChEMBL	Inhibition of human carbonic anhydrase 2 preincubated for 15 mins by CO2 hydrase stopped flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1253-1259 [PMID:26832216]
ChEMBL	Inhibition of human carbonic anhydrase 2 incubated for 15 mins by stopped-flow CO2 hydration assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 1821-1826 [PMID:26920803]
ChEMBL	Inhibition of human carbonic anhydrase 2 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 sec by Line-Weaver Burk plot analysis	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2017) 27: 490-495 [PMID:28025002]
ChEMBL	Inhibition of human carbonic anhydrase-2 assessed as reduction in CO2 hydration preincubated for 15 mins followed by CO2 addition measured for 10 to 100 secs by stopped-flow assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem (2017) 25: 3555-3561 [PMID:28511911]
ChEMBL	Inhibition of human cytosolic carbonic anhydrase 2 preincubated for 15 mins by stop flow CO2 hydrase assay	B	6.54	pKi	290	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4184-4190 [PMID:27485387]
ChEMBL	Binding affinity to human recombinant CA II assessed as inhibition constant	B	6.54	pKi	290	nM	Ki	J Med Chem (2024) 67: 15862-15872 [PMID:39161321]
Carbonic anhydrase, alpha family in Thiomicrospira crunogena (strain XCL-2) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3621035] [UniProtKB: Q31FD6]
ChEMBL	Inhibition of recombinant Thiomicrospira crunogena XCL-2 carbonic anhydrase by stopped flow CO2 hydrase assay	B	6.53	pKi	296	nM	Ki	Bioorg Med Chem Lett (2016) 26: 401-405 [PMID:26691758]
Delta carbonic anhydrase in Thalassiosira weissflogii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3097982] [UniProtKB: Q5U9J1]
ChEMBL	Inhibition of Thalassiosira weissflogii delta carbonic anhydrase preincubated for 15 mins by stopped flow CO2 hydration assay	B	5.37	pKi	4270	nM	Ki	Bioorg Med Chem Lett (2014) 24: 275-279 [PMID:24314394]
N-Acylphosphatidylethanolamine-phospholipase D in Human [GtoPdb: 1398] [UniProtKB: Q6IQ20]
GtoPdb	Determined by surface plasmon resonance, in the absence of deoxycholic acid in the bile acid binding domain	-	5.1	pKd	8000	nM	Kd	Cell Chem Biol (2025) 32: 449-462.e5 [PMID:39999832]
GtoPdb	equilibrium dissociation constant in presence of deoxycholic acid, determined by SPR	-	6.13	pKd	740	nM	Kd	Cell Chem Biol (2025) 32: 449-462.e5 [PMID:39999832]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]