adenosine [Ligand Id: 2844] activity data from GtoPdb and ChEMBL
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| ChEMBL ligand: CHEMBL477 (9-.beta.-d-ribofuranosyladenine, Adenocard, Adenocor, Adenoscan, Adenosine, NSC-7652, SR 96225, SR-96225) |
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| DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
|---|---|---|---|---|---|---|---|---|
| A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] | ||||||||
| GtoPdb | - | - | 7 | pKi | ~100 | nM | Ki | Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Agonist activity at human Adenosine A1 receptor transfected in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by multimode microplate reader analysis | F | 8.63 | pIC50 | 2.34 | nM | IC50 | J Med Chem (2016) 59: 947-964 [PMID:26756468] |
| ChEMBL | Agonist activity at human Adenosine A1 receptor expressed in yeast cells coexpressed with chimeric GPA1/Galphai1 after 16 hrs by beta galactosidase reporter gene assay | B | 5.6 | pEC50 | 2511.89 | nM | EC50 | J Med Chem (2016) 59: 947-964 [PMID:26756468] |
| ChEMBL | Agonist activity at human A1AR assessed as induction of Ca2+ ion mobilization by orthogonal functional assay | F | 5.85 | pEC50 | 1410 | nM | EC50 | J Med Chem (2012) 55: 6467-6477 [PMID:22738238] |
| ChEMBL | Agonist activity at human adenosine A1 receptor transfected in CHO cells assessed as changes in cAMP formation in the presence of nitrobenzylthioinosine | F | 6.51 | pEC50 | 310 | nM | EC50 | J Med Chem (2016) 59: 788-809 [PMID:26356532] |
| ChEMBL | Agonist activity at human adenosine A1 receptor expressed in CHO cells | F | 6.51 | pEC50 | 310 | nM | EC50 | Bioorg Med Chem Lett (2011) 21: 1933-1936 [PMID:21388809] |
| ChEMBL | Agonist activity at human adenosine A1 receptor expressed in CHO cells assessed as increase of intracellular calcium level | F | 6.51 | pEC50 | 310 | nM | EC50 | Bioorg Med Chem Lett (2010) 20: 4140-4146 [PMID:20541935] |
| ChEMBL | Agonist activity at human recombinant adenosine A1 receptor by cAMP assay | F | 6.51 | pEC50 | 310 | nM | EC50 | J Med Chem (2014) 57: 3623-3650 [PMID:24164628] |
| ChEMBL | Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay | F | 7.41 | pEC50 | 39 | nM | EC50 | J Med Chem (2012) 55: 6467-6477 [PMID:22738238] |
| A1 receptor/Adenosine A1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099] | ||||||||
| ChEMBL | Binding affinity to adenosine A1 receptor in rat brain membranes by measuring displacement of specific [3H]PIA as radioligand. | B | 4.54 | pKi | 29000 | nM | Ki | J Med Chem (1995) 38: 1174-1188 [PMID:7707320] |
| GtoPdb | - | - | 7.14 | pKi | 73 | nM | Ki | Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Inhibition of binding of [3H]N6-cyclohexyladenosine to adenosine A1 receptor of rat whole brain membranes. | B | 7.89 | pKi | 12.8 | nM | Ki | J Med Chem (1989) 32: 1667-1673 [PMID:2754691] |
| ChEMBL | Affinity for Adenosine A1 receptor determined by [3H]N6-cyclohexyladenosine binding to rat brain membranes | B | 7.89 | pKi | 12.8 | nM | Ki | Bioorg Med Chem Lett (1991) 1: 481-486 |
| ChEMBL | Displacement of [3H]R-PIA from rat brain membrane Adenosine A1 receptor | B | 8 | pKi | 10 | nM | Ki | J Med Chem (2000) 43: 2196-2203 [PMID:10841798] |
| ChEMBL | Evaluated for the binding affinity towards the Adenosine A1 receptor in corpora striata of rats using [3H]CHA as radioligand. | B | 8 | pKi | 10 | nM | Ki | J Med Chem (1990) 33: 2240-2254 [PMID:2374150] |
| ChEMBL | Inhibition of [3H]-CHA binding to rat brain membrane Adenosine A1 receptor | B | 8.29 | pKi | 5.1 | nM | Ki | J Med Chem (1990) 33: 3127-3130 [PMID:2258897] |
| Adenosine A1 receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2304404] [UniProtKB: P47745] | ||||||||
| ChEMBL | Effective concentration required for prolongation of the stimulus-QRS interval by 50% of the maximum response at the A1 adenosine receptor in langendorff guinea pig heart preparation | F | 5.47 | pEC50 | 3388.44 | nM | EC50 | J Med Chem (1991) 34: 1340-1344 [PMID:2016708] |
| ChEMBL | Prolongation of the stimulus-QRS interval by 50% of the maximum response at the adenosine A1 receptor in langendorff guinea pig heart preparation | F | 5.47 | pEC50 | 3388.44 | nM | EC50 | J Med Chem (1991) 34: 1334-1339 [PMID:2016707] |
| A2A receptor/Adenosine A2a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274] | ||||||||
| ChEMBL | Binding affinity to human recombinant adenosine receptor A2A expressed in CHO cells assessed as inhibitory constant by radioligand competition assay | B | 6.15 | pKi | 700 | nM | Ki | Eur J Med Chem (2019) 179: 310-324 [PMID:31255928] |
| GtoPdb | - | - | 6.51 | pKi | 310 | nM | Ki |
Annu Rev Pharmacol Toxicol (2005) 45: 385-412 [PMID:15822182]; Pharmacol Toxicol (1995) 76: 93-101 [PMID:7746802]; Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Binding affinity to A2A adenosine receptor | B | 7.7 | pKi | 20 | nM | Ki | J Med Chem (2012) 55: 538-552 [PMID:22104008] |
| ChEMBL | Effective concentration required for cyclic AMP dependent inhibition of blood platelet aggregation for A2 receptor stimulation | B | 4.4 | pEC50 | 40000 | nM | EC50 | J Med Chem (1993) 36: 4113-4120 [PMID:8258836] |
| ChEMBL | Agonist activity at human Adenosine A2A receptor expressed in yeast cells coexpressed with chimeric GPA1/Galphas after 16 hrs by beta-galactosidase reporter gene assay | B | 5.24 | pEC50 | 5754.4 | nM | EC50 | J Med Chem (2016) 59: 947-964 [PMID:26756468] |
| ChEMBL | Agonist activity at human adenosine A2A receptor transfected in CHO cells assessed as changes in cAMP formation in the presence of nitrobenzylthioinosine | F | 6.14 | pEC50 | 730 | nM | EC50 | J Med Chem (2016) 59: 788-809 [PMID:26356532] |
| ChEMBL | Agonist activity at human adenosine A2A receptor expressed in CHO cells assessed as increase of intracellular calcium level | F | 6.15 | pEC50 | 700 | nM | EC50 | Bioorg Med Chem Lett (2010) 20: 4140-4146 [PMID:20541935] |
| ChEMBL | Agonist activity at human adenosine A2A receptor expressed in CHO cells | F | 6.15 | pEC50 | 700 | nM | EC50 | Bioorg Med Chem Lett (2011) 21: 1933-1936 [PMID:21388809] |
| ChEMBL | Agonist activity at human recombinant adenosine receptor A2a by cAMP assay | F | 6.15 | pEC50 | 700 | nM | EC50 | J Med Chem (2014) 57: 3623-3650 [PMID:24164628] |
| A2A receptor/Adenosine A2a receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543] | ||||||||
| GtoPdb | - | - | 6.82 | pKi | 150 | nM | Ki | Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Displacement of specific [3H]-CGS- 21680 binding to adenosine A2A receptor in rat striatal membranes | B | 7.52 | pKi | 30 | nM | Ki | J Med Chem (2000) 43: 2196-2203 [PMID:10841798] |
| A2B receptor/Adenosine A2b receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275] | ||||||||
| GtoPdb | - | - | 4.82 | pKi | 15000 | nM | Ki |
Annu Rev Pharmacol Toxicol (2005) 45: 385-412 [PMID:15822182]; Pharmacol Toxicol (1995) 76: 93-101 [PMID:7746802]; Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Agonist activity at human Adenosine A2B receptor expressed in yeast cells coexpressed with chimeric GPA1/Galphas after 16 hrs by beta-galactosidase reporter gene assay | B | 4.29 | pEC50 | 51286.14 | nM | EC50 | J Med Chem (2016) 59: 947-964 [PMID:26756468] |
| ChEMBL | Agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as increase of intracellular calcium level | F | 4.62 | pEC50 | 24000 | nM | EC50 | Bioorg Med Chem Lett (2010) 20: 4140-4146 [PMID:20541935] |
| ChEMBL | Agonist activity at human adenosine A2B receptor expressed in CHO cells | F | 4.62 | pEC50 | 24000 | nM | EC50 | Bioorg Med Chem Lett (2011) 21: 1933-1936 [PMID:21388809] |
| ChEMBL | Agonist activity at human recombinant adenosine receptor A2b by cAMP assay | F | 4.62 | pEC50 | 24000 | nM | EC50 | J Med Chem (2014) 57: 3623-3650 [PMID:24164628] |
| ChEMBL | Agonist activity at A2B receptor (unknown origin) | B | 4.62 | pEC50 | 24000 | nM | EC50 | J Med Chem (2021) 64: 458-480 [PMID:33372800] |
| ChEMBL | Agonist activity at human adenosine A2B receptor transfected in CHO cells assessed as changes in cAMP formation in the presence of nitrobenzylthioinosine | F | 4.63 | pEC50 | 23500 | nM | EC50 | J Med Chem (2016) 59: 788-809 [PMID:26356532] |
| A2B receptor in Rat [GtoPdb: 20] [UniProtKB: P29276] | ||||||||
| GtoPdb | - | - | 5.29 | pKi | 5100 | nM | Ki | Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| A3 receptor/Adenosine A3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8] | ||||||||
| GtoPdb | - | - | 6.54 | pKi | 290 | nM | Ki |
Annu Rev Pharmacol Toxicol (2005) 45: 385-412 [PMID:15822182]; Pharmacol Toxicol (1995) 76: 93-101 [PMID:7746802]; Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Agonist activity at human Adenosine A3 receptor transfected in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP production after 30 mins by multimode microplate reader analysis | F | 8.94 | pIC50 | 1.15 | nM | IC50 | J Med Chem (2016) 59: 947-964 [PMID:26756468] |
| ChEMBL | Activity against human wild type adenosine A3 receptor expressed in COS7 cells as measured by accumulation of inositol phosphate by PLC assay | F | 5.98 | pEC50 | 1040 | nM | EC50 | J Med Chem (2006) 49: 2689-2702 [PMID:16640329] |
| ChEMBL | Agonist activity at human adenosine A3 receptor expressed in CHO cells assessed as increase of intracellular calcium level | F | 6.54 | pEC50 | 290 | nM | EC50 | Bioorg Med Chem Lett (2010) 20: 4140-4146 [PMID:20541935] |
| ChEMBL | Agonist activity at human adenosine A3 receptor expressed in CHO cells | F | 6.54 | pEC50 | 290 | nM | EC50 | Bioorg Med Chem Lett (2011) 21: 1933-1936 [PMID:21388809] |
| ChEMBL | Agonist activity at human recombinant adenosine A3 receptor by cAMP assay | F | 6.54 | pEC50 | 290 | nM | EC50 | J Med Chem (2014) 57: 3623-3650 [PMID:24164628] |
| ChEMBL | Agonist activity at human adenosine A3 receptor transfected in CHO cells assessed as changes in cAMP formation in the presence of nitrobenzylthioinosine | F | 6.54 | pEC50 | 290 | nM | EC50 | J Med Chem (2016) 59: 788-809 [PMID:26356532] |
| A3 receptor/Adenosine A3 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647] | ||||||||
| GtoPdb | - | - | 5.19 | pKi | 6500 | nM | Ki | Expert Opin Emerg Drugs (2003) 8: 537-76 [PMID:14662005] |
| ChEMBL | Displacement of [125I]- AB-MECA from rat adenosine A3 receptor expressed in CHO cells | B | 7 | pKi | 100 | nM | Ki | J Med Chem (2000) 43: 2196-2203 [PMID:10841798] |
| Adenosine kinase in Toxoplasma gondii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2982] [UniProtKB: Q9TVW2] | ||||||||
| ChEMBL | Binding affinity to purified Toxoplasma gondii adenosine kinase | B | 5.05 | pKi | 8900 | nM | Ki | J Med Chem (2008) 51: 3934-3945 [PMID:18563892] |
| cAMP-dependent protein kinase A in Bovine (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2111446] [UniProtKB: P00515, P00517, P05131, P31322] | ||||||||
| ChEMBL | Inhibitory constant for Protein Kinase A | B | 4.82 | pKi | 15000 | nM | Ki | Bioorg Med Chem Lett (1996) 6: 899-902 |
| heat shock protein 90 beta family member 1/Endoplasmin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075323] [GtoPdb: 2904] [UniProtKB: P14625] | ||||||||
| ChEMBL | Displacement of FITC-geldanamycin from GRP94 (unknown origin) after 24 hrs by fluorescence polarization assay | B | 4.3 | pKd | >50000 | nM | Kd | ACS Med Chem Lett (2021) 12: 373-379 [PMID:33738064] |
| ChEMBL | Displacement of FITC-geldanamycin from GRP94 (unknown origin) after 24 hrs by fluorescence polarization assay | B | 5 | pKi | >10000 | nM | Ki | ACS Med Chem Lett (2021) 12: 373-379 [PMID:33738064] |
| Glyceraldehyde-3-phosphate dehydrogenase liver in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2284] [UniProtKB: P04406] | ||||||||
| ChEMBL | Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of Trypanosoma brucei | B | 4 | pIC50 | 100000 | nM | IC50 | J Med Chem (1994) 37: 3605-3613 [PMID:7932587] |
| ChEMBL | Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of Leishmania mexicana | B | 4.3 | pIC50 | 50000 | nM | IC50 | J Med Chem (1994) 37: 3605-3613 [PMID:7932587] |
| ChEMBL | Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of human erythrocyte | B | 4.46 | pIC50 | 35000 | nM | IC50 | J Med Chem (1994) 37: 3605-3613 [PMID:7932587] |
| DOT1 like histone lysine methyltransferase/Histone-lysine N-methyltransferase, H3 lysine-79 specific in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1795117] [GtoPdb: 2650] [UniProtKB: Q8TEK3] | ||||||||
| ChEMBL | Inhibition of DOT1L (2 to 416 residues) (unknown origin) using biotinylated nucleosomes as substrate preincubated for 30 mins followed by substrate addition in presence of [3H-Me]SAM by scintillation proximity assay | B | 4 | pIC50 | 100000 | nM | IC50 | ACS Med Chem Lett (2017) 8: 338-343 [PMID:28337327] |
| inosine monophosphate dehydrogenase 1/Inosine-5`-monophosphate dehydrogenase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1822] [GtoPdb: 2624] [UniProtKB: P20839] | ||||||||
| ChEMBL | Inhibition of human IMP dehydrogenase 1 | B | 4 | pKi | >100000 | nM | Ki | Bioorg Med Chem (2008) 16: 7462-7469 [PMID:18583139] |
| inosine monophosphate dehydrogenase 2/Inosine-5`-monophosphate dehydrogenase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2002] [GtoPdb: 2625] [UniProtKB: P12268] | ||||||||
| ChEMBL | Inhibition of human IMP dehydrogenase 2 | B | 4 | pKi | >100000 | nM | Ki | Bioorg Med Chem (2008) 16: 7462-7469 [PMID:18583139] |
| mitogen-activated protein kinase kinase kinase 7/Mitogen-activated protein kinase kinase kinase 7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5776] [GtoPdb: 2082] [UniProtKB: O43318] | ||||||||
| ChEMBL | Binding affinity to recombinant human biotinylated N-terminal GST-tagged non-autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay | B | 4.47 | pKd | 34000 | nM | Kd | Bioorg Med Chem Lett (2017) 27: 1031-1036 [PMID:28109791] |
| ChEMBL | Binding affinity to recombinant human biotinylated N-terminal GST-tagged autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay | B | 5.36 | pKd | 4400 | nM | Kd | Bioorg Med Chem Lett (2017) 27: 1031-1036 [PMID:28109791] |
| TRPM4 in Human [GtoPdb: 496] [UniProtKB: Q8TD43] | ||||||||
| GtoPdb | - | - | 3.2 | pIC50 | 630000 | nM | IC50 | |
| phosphatidylinositol 4-kinase type 2 alpha in Human [GtoPdb: 2498] [UniProtKB: Q9BTU6] | ||||||||
| GtoPdb | - | - | 5 | pIC50 | - | - | - | Anal Biochem (2011) 417: 97-102 [PMID:21704602] |
| phosphatidylinositol 4-kinase type 2 beta in Human [GtoPdb: 2499] [UniProtKB: Q8TCG2] | ||||||||
| GtoPdb | - | - | 5 | pIC50 | - | - | - | Anal Biochem (2011) 417: 97-102 [PMID:21704602] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]
