ChEMBL ligand: CHEMBL107 (Colchcine, (-)-colchicine, Colchicine, Colchicinum, Colchineos, Colchisol, Colcrys, Gloperba, Mitigare, NSC-757)
ABCB1/P-glycoprotein 1 in Human [ChEMBL: CHEMBL4302] [GtoPdb: 768] [UniProtKB: P08183] P-glycoprotein 1 in Mouse [ChEMBL: CHEMBL3467] [UniProtKB: P06795] There should be some charts here, you may need to enable JavaScript!
ABCB1/P-glycoprotein 3 in Mouse [ChEMBL: CHEMBL2573] [GtoPdb: 768] [UniProtKB: P21447] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Pim-1 proto-oncogene, serine/threonine kinase/Serine/threonine-protein kinase PIM1 in Human [ChEMBL: CHEMBL2147] [GtoPdb: 2158] [UniProtKB: P11309] There should be some charts here, you may need to enable JavaScript!
Organic cation transporter 3/Solute carrier family 22 member 3 in Human [ChEMBL: CHEMBL2073673] [GtoPdb: 1021] [UniProtKB: O75751] There should be some charts here, you may need to enable JavaScript!
tubulin beta class I/tubulin alpha 4a/tubulin beta 4B class IVb/tubulin beta 3 class III/tubulin beta 8 class VIII/tubulin alpha 1a/Tubulin in Human [ChEMBL: CHEMBL2095182] [GtoPdb: 2640, 2639, 2641, 2752, 2753, 2638] [UniProtKB: P04350, P07437, P0DPH7, P68363, P68366, P68371, Q13509, Q13885, Q3ZCM7, Q6PEY2, Q71U36, Q9BQE3, Q9BUF5, Q9BVA1, Q9H4B7] Tubulin in Bovine [ChEMBL: CHEMBL2111464] [UniProtKB: Q6B856, Q862F3] There should be some charts here, you may need to enable JavaScript!
Tubulin alpha chain in Pig [ChEMBL: CHEMBL3658] [UniProtKB: P02550] There should be some charts here, you may need to enable JavaScript!
tubulin beta class I/Tubulin beta-5 chain in Human [ChEMBL: CHEMBL5444] [GtoPdb: 2640] [UniProtKB: P07437] There should be some charts here, you may need to enable JavaScript!
Tubulin beta chain in Bovine [ChEMBL: CHEMBL3394] [UniProtKB: Q6B856] There should be some charts here, you may need to enable JavaScript!
Tubulin polymerization-promoting protein in Bovine [ChEMBL: CHEMBL2366491] [UniProtKB: Q27957] There should be some charts here, you may need to enable JavaScript!
glycine receptor α1 subunit in Human [GtoPdb: 423] [UniProtKB: P23415] There should be some charts here, you may need to enable JavaScript!
glycine receptor α2 subunit in Human [GtoPdb: 424] [UniProtKB: P23416] There should be some charts here, you may need to enable JavaScript!
TAS2R4 in Human [GtoPdb: 661] [UniProtKB: Q9NYW5] There should be some charts here, you may need to enable JavaScript!
TAS2R46 in Human [GtoPdb: 679] [UniProtKB: P59540] There should be some charts here, you may need to enable JavaScript!
bromodomain containing 4 in Human [GtoPdb: 1945] [UniProtKB: O60885] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
ABCB1/P-glycoprotein 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4302] [GtoPdb: 768] [UniProtKB: P08183]
ChEMBL	Inhibition of P-glycoprotein using calcein-AM assay transfected in porcine PBCEC	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
ChEMBL	Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
ChEMBL	TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
P-glycoprotein 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3467] [UniProtKB: P06795]
ChEMBL	Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
ChEMBL	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
ABCB1/P-glycoprotein 3 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2573] [GtoPdb: 768] [UniProtKB: P21447]
ChEMBL	Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
ChEMBL	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells	F	4.3	pIC50	>50000	nM	IC50	J Med Chem (2003) 46: 1716-1725 [PMID:12699389]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum D10 after 72 hrs by SYBR green assay	F	4.9	pIC50	12589.25	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay	F	4.9	pIC50	12589.25	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum HB3 after 72 hrs by SYBR green assay	F	4.9	pIC50	12589.25	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay	F	5	pIC50	10000	nM	IC50	Nat Chem Biol (2009) 5: 765-771 [PMID:19734910]
Pim-1 proto-oncogene, serine/threonine kinase/Serine/threonine-protein kinase PIM1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2147] [GtoPdb: 2158] [UniProtKB: P11309]
ChEMBL	Inhibition of human PIM1 measured after 5 mins by ELISA relative to control	B	5.37	pIC50	4300	nM	IC50	Eur J Med Chem (2017) 134: 357-365 [PMID:28431341]
Organic cation transporter 3/Solute carrier family 22 member 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2073673] [GtoPdb: 1021] [UniProtKB: O75751]
ChEMBL	TP_TRANSPORTER: inhibition of MPP+ uptake in OCT3-expressing HEK293 cells	F	6.92	pKi	120	nM	Ki	Nat Neurosci (1998) 1: 349-351 [PMID:10196521]
tubulin beta class I/tubulin alpha 4a/tubulin beta 4B class IVb/tubulin beta 3 class III/tubulin beta 8 class VIII/tubulin alpha 1a/Tubulin in Human (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2095182] [GtoPdb: 2640, 2639, 2641, 2752, 2753, 2638] [UniProtKB: P04350, P07437, P0DPH7, P68363, P68366, P68371, Q13509, Q13885, Q3ZCM7, Q6PEY2, Q71U36, Q9BQE3, Q9BUF5, Q9BVA1, Q9H4B7]
ChEMBL	Binding affinity to tubulin (unknown origin) assessed as dissociation constant by SPR analysis	B	4.95	pKd	11300	nM	Kd	Eur J Med Chem (2019) 170: 73-86 [PMID:30878833]
ChEMBL	Binding affinity to tubulin (unknown origin) by ITC method	B	7.24	pKd	58	nM	Kd	Eur J Med Chem (2017) 141: 460-472 [PMID:29055869]
ChEMBL	Displacement of [3H]-colchicine from biotin-labeled tubulin (unknown origin) after 2 hrs by scintillation proximity assay	B	5.62	pKi	2400	nM	Ki	J Med Chem (2013) 56: 8008-8018 [PMID:24106982]
ChEMBL	Inhibition of brain tubulin (unknown origin) polymerization measured up to 15 to 30 mins	B	5.11	pIC50	7800	nM	IC50	J Med Chem (2015) 58: 6678-6696 [PMID:26226379]
ChEMBL	Inhibition of tubulin (unknown origin) polymerization by spectrophotometry	B	5.27	pIC50	5400	nM	IC50	Bioorg Med Chem (2014) 22: 4917-4923 [PMID:25059503]
ChEMBL	In vitro inhibition of tubulin polymerization.	F	5.38	pIC50	4200	nM	IC50	J Med Chem (1993) 36: 1474-1479 [PMID:8496915]
ChEMBL	Inhibition of tubulin polymerization	F	5.48	pIC50	3300	nM	IC50	J Med Chem (2006) 49: 6656-6659 [PMID:17154496]
ChEMBL	Inhibition of tubulin (unknown origin) polymerization incubated 15 mins prior to GTP addition by spectrophotometry	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2014) 57: 6531-6552 [PMID:25025991]
ChEMBL	Inhibition of tubulin polymerization	F	5.49	pIC50	3200	nM	IC50	J Med Chem (2006) 49: 6412-6415 [PMID:17034147]
ChEMBL	Inhibition of tubulin polymerization	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2004) 47: 6120-6123 [PMID:15566282]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) incubated for 15 mins followed by GTP addition and measured for 20 mins by spectrophotometry	B	5.49	pIC50	3200	nM	IC50	Eur J Med Chem (2020) 185: 111828-111828 [PMID:31727471]
ChEMBL	Inhibition of tubulin polymerization	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2007) 50: 2865-2874 [PMID:17497841]
ChEMBL	Inhibition of tubulin (unknown origin) polymerization preincubated for 15 mins by spectrophotometry	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2013) 56: 123-149 [PMID:23214452]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) preincubated for 15 mins followed by GTP addition measured for 20 mins by spectrophotometric method	B	5.49	pIC50	3200	nM	IC50	Eur J Med Chem (2018) 152: 283-297 [PMID:29730191]
ChEMBL	Inhibition of tubulin (unknown origin) assembly preincubated for 15 mins followed by GTP addition measured for 20 mins by spectrophotometric analysis	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2015) 58: 5789-5807 [PMID:26132075]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) measured every 30 secs for 90 mins	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2021) 40: 127968-127968 [PMID:33753264]
ChEMBL	Inhibition of tubulin polymerization in presence of microtubule associated proteins after 30 mins	B	5.54	pIC50	2900	nM	IC50	J Med Chem (2007) 50: 4548-4552 [PMID:17685504]
ChEMBL	Inhibition of tubulin polymerization	B	5.57	pIC50	2700	nM	IC50	J Med Chem (2006) 49: 2357-2360 [PMID:16570932]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) by DAPI staining-based fluorescence microscopy	B	5.59	pIC50	2600	nM	IC50	J Med Chem (2014) 57: 6364-6382 [PMID:25061803]
ChEMBL	Displacement of [3H]-colchicine from tubulin (unknown origin) preincubated with protein for 1 hr followed by [3H]-colchicine addition measured after 3 hrs by scintillation counting analysis	B	5.66	pIC50	2210	nM	IC50	J Med Chem (2015) 58: 6549-6558 [PMID:26241032]
ChEMBL	Inhibition of tubulin polymerization	F	5.72	pIC50	1900	nM	IC50	J Med Chem (1993) 36: 1146-1156 [PMID:8387598]
ChEMBL	Inhibition of microtubule polymerization	F	5.77	pIC50	1700	nM	IC50	Bioorg Med Chem Lett (2006) 16: 2761-2764 [PMID:16504507]
ChEMBL	Inhibition of Tubulin polymerization after 20 minutes of administration at 30 degree Centigrade	F	5.82	pIC50	1500	nM	IC50	J Med Chem (1997) 40: 961-966 [PMID:9083485]
ChEMBL	In vitro inhibition of mammalian tubulin polymerization by 50%	B	5.82	pIC50	1500	nM	IC50	J Med Chem (1998) 41: 1956-1961 [PMID:9599244]
ChEMBL	Inhibition of tubulin polymerization (unknown origin)	B	5.82	pIC50	1500	nM	IC50	J Med Chem (2020) 63: 10618-10651 [PMID:32432867]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) incubated for 20 mins	B	5.82	pIC50	1500	nM	IC50	J Med Chem (2020) 63: 10618-10651 [PMID:32432867]
ChEMBL	Inhibition of tubulin polymerization in human K562 cells	B	5.85	pIC50	1400	nM	IC50	Eur J Med Chem (2010) 45: 3354-3364 [PMID:20546980]
ChEMBL	In vitro inhibitory activity against tubulin polymerization (10 uM)	B	5.85	pIC50	1400	nM	IC50	J Med Chem (1998) 41: 2333-2338 [PMID:9632366]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) preincubated for 10 mins followed by GTP addition measured after 20 mins by Coomassie blue staining based SDS-PAGE analysis	B	5.88	pIC50	1320	nM	IC50	Eur J Med Chem (2017) 141: 460-472 [PMID:29055869]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) relative to control	B	5.89	pIC50	1280	nM	IC50	Bioorg Med Chem (2020) 28: 115674-115674 [PMID:33065442]
ChEMBL	Displacement of [3H]colchicine from biotinylated tubulin by scintillation proximity assay	B	5.98	pIC50	1050	nM	IC50	J Med Chem (2007) 50: 6059-6066 [PMID:17973361]
ChEMBL	Inhibition of [3H]colchicine binding to beta tubulin	B	6.3	pIC50	500	nM	IC50	J Med Chem (2006) 49: 5769-5776 [PMID:16970401]
ChEMBL	Inhibition of tubulin (unknown origin) polymerization preincubated for 45 mins followed by GTP addition measured after 2 hrs by fluorescence assay	B	6.44	pIC50	360	nM	IC50	Eur J Med Chem (2015) 93: 93-100 [PMID:25659770]
ChEMBL	Inhibition of tubulin polymerization (unknown origin) incubated for 1 hr by fluorescence based assay	B	6.72	pIC50	190	nM	IC50	RSC Med Chem (2020) 11: 327-348 [PMID:33479639]
GtoPdb	-	-	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2006) 16: 2761-4 [PMID:16504507]
ChEMBL	Inhibition of tubulin polymerization in human HeLa cells assessed as dynamic tyrosinated microtubules content by incubated for 2 hrs by immunofluorescence staining based assay	B	6.7	pEC50	200	nM	EC50	Eur J Med Chem (2021) 215: 113275-113275 [PMID:33618157]
Tubulin in Bovine (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111464] [UniProtKB: Q6B856, Q862F3]
ChEMBL	Inhibition of bovine brain tubulin polymerization extent after 20 mins by turbidimetrically	B	5.58	pIC50	2620	nM	IC50	Eur J Med Chem (2015) 99: 125-137 [PMID:26070164]
ChEMBL	Inhibition of bovine brain tubulin polymerization after 30 mins	B	5.72	pIC50	1900	nM	IC50	Bioorg Med Chem (2015) 23: 4230-4236 [PMID:26160020]
Tubulin alpha chain in Pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3658] [UniProtKB: P02550]
ChEMBL	Compound was evaluated for its ability to inhibit the binding of [3H]-colchicine to tubulin in competitive binding assay	B	5.6	pKi	2500	nM	Ki	Bioorg Med Chem Lett (1991) 1: 471-476
ChEMBL	Inhibition of tubulin polymerization interacting at the colchicine binding site.	B	5.38	pIC50	4168.69	nM	IC50	J Med Chem (2000) 43: 167-176 [PMID:10649972]
ChEMBL	Tested for 50 % inhibition of tubulin polymerization by turbidimetric assay	B	5.72	pIC50	1900	nM	IC50	Bioorg Med Chem Lett (1993) 3: 581-584
ChEMBL	The compound was evaluated in vitro for the mammalian brain tubulin polymerization using standard assay.	F	5.82	pIC50	1500	nM	IC50	Bioorg Med Chem Lett (1992) 2: 1257-1262
ChEMBL	Inhibition of tubulin polymerization (ITP)	B	6.1	pIC50	800	nM	IC50	J Med Chem (2001) 44: 3932-3936 [PMID:11689079]
tubulin beta class I/Tubulin beta-5 chain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5444] [GtoPdb: 2640] [UniProtKB: P07437]
ChEMBL	Inhibition of tubulin beta polymerization in human Caco2 cells after 48 hrs by ELISA	B	5.11	pIC50	7710	nM	IC50	Medchemcomm (2018) 9: 1511-1528 [PMID:30288225]
ChEMBL	Inhibition of tubulin beta polymerization in human MCF7 cells after 18 to 24 hrs by ELISA based spectrophotometric analysis	B	5.16	pIC50	6930	nM	IC50	Bioorg Med Chem (2018) 26: 3474-3490 [PMID:29793751]
ChEMBL	Inhibition of tubulin beta polymerization in human MCF7 cells after 48 hrs by ELISA	B	5.34	pIC50	4590	nM	IC50	Medchemcomm (2018) 9: 1511-1528 [PMID:30288225]
GtoPdb	-	-	7.96	pIC50	11	nM	IC50	Bioorg Med Chem Lett (2006) 16: 2761-4 [PMID:16504507]
Tubulin beta chain in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3394] [UniProtKB: Q6B856]
ChEMBL	Binding constant at colchicine site of bovine brain tubulin	B	6.11	pKi	780	nM	Ki	Bioorg Med Chem Lett (2002) 12: 465-469 [PMID:11814821]
ChEMBL	Compound was evaluated for in vitro inhibitory activity against polymerization of tubulin into microtubules isolated from bovine brain at 1 mg/mL tubulin concentration	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem Lett (1993) 3: 565-570
ChEMBL	Inhibitory effect on the polymerization of purified bovine brain tubulin.	F	5.62	pIC50	2400	nM	IC50	J Med Chem (1990) 33: 567-571 [PMID:2299625]
ChEMBL	Inhibition of tubulin polymerization	F	6.1	pIC50	800	nM	IC50	J Med Chem (1994) 37: 1126-1135 [PMID:8164254]
ChEMBL	Inhibition of bovine tubulin polymerization	F	6.1	pIC50	800	nM	IC50	J Med Chem (1997) 40: 2266-2275 [PMID:9216846]
Tubulin polymerization-promoting protein in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2366491] [UniProtKB: Q27957]
ChEMBL	Inhibition of Bos taurus (bovine) brain tubulin polymerization	B	6.12	pEC50	750	nM	EC50	Bioorg Med Chem Lett (2001) 11: 1393-1396 [PMID:11378362]
glycine receptor α1 subunit in Human [GtoPdb: 423] [UniProtKB: P23415]
GtoPdb	-	-	3.5	pIC50	324000	nM	IC50
glycine receptor α2 subunit in Human [GtoPdb: 424] [UniProtKB: P23416]
GtoPdb	-	-	4.2	pIC50	64000	nM	IC50
TAS2R4 in Human [GtoPdb: 661] [UniProtKB: Q9NYW5]
GtoPdb	-	-	2.99	pEC50	1025000	nM	EC50	Chem Senses (2010) 35: 157-70 [PMID:20022913]
TAS2R46 in Human [GtoPdb: 679] [UniProtKB: P59540]
GtoPdb	-	-	2.8	pEC50	1580000	nM	EC50	Chem Senses (2010) 35: 157-70 [PMID:20022913]
bromodomain containing 4 in Human [GtoPdb: 1945] [UniProtKB: O60885]
GtoPdb	-	-	4.7	pKd	20000	nM	Kd	Angew Chem Int Ed Engl (2013) 52: 14055-9 [PMID:24272870]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]