ChEMBL ligand: CHEMBL203125 (Fosmidomycin)
1-deoxy-D-xylulose 5-phosphate reductoisomerase in Escherichia coli [ChEMBL: CHEMBL3421521] [UniProtKB: W8T2T2] 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Mycobacterium tuberculosis [ChEMBL: CHEMBL5630] [UniProtKB: P9WNS1] 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [ChEMBL: CHEMBL2010623] [UniProtKB: A0QVH7] There should be some charts here, you may need to enable JavaScript!
1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic in Arabidopsis thaliana [ChEMBL: CHEMBL2285354] [UniProtKB: Q9XFS9] There should be some charts here, you may need to enable JavaScript!
1-deoxyxylulose-5-phosphate reductoisomerase in Escherichia coli [ChEMBL: CHEMBL4091] [UniProtKB: P45568] 1-deoxyxylulose-5-phosphate reductoisomerase in Synechocystis sp. (strain PCC 6803 / Kazusa) [ChEMBL: CHEMBL4094] [UniProtKB: Q55663] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum A2 [GtoPdb: 2980] Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum Dd2 [GtoPdb: 2980] Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum HB3 [GtoPdb: 2980] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
1-deoxy-D-xylulose 5-phosphate reductoisomerase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3421521] [UniProtKB: W8T2T2]
ChEMBL	Inhibition of recombinant Escherichia coli Dxr expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells using DOXP as substrate assessed as NADPH oxidation preincubated for 5 mins with NADPH followed by substrate addition by spectrophotometric analysis	B	6.36	pIC50	438	nM	IC50	J Med Chem (2015) 58: 2988-3001 [PMID:25781377]
1-deoxy-D-xylulose 5-phosphate reductoisomerase in Mycobacterium tuberculosis (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5630] [UniProtKB: P9WNS1]
ChEMBL	Binding affinity to Mycobacterium tuberculosis DXR assessed as dissociation constant by spectrofluorimetric analysis in presence of NADPH and MnCl2	B	7.4	pKd	40	nM	Kd	J Med Chem (2013) 56: 6190-6199 [PMID:23819803]
ChEMBL	Inhibition of Mycobacterium tuberculosis recombinant DXR expressed in Escherichia coli BL21 (DE3) using DXP as substrate and MgCl2 as cofactor preincubated for 10 mins	B	6.85	pKi	140	nM	Ki	J Med Chem (2011) 54: 4721-4734 [PMID:21561155]
ChEMBL	Inhibition of Mycobacterium tuberculosis Dxr	B	6.36	pIC50	440	nM	IC50	Bioorg Med Chem Lett (2014) 24: 649-653 [PMID:24360562]
ChEMBL	Inhibition of Mycobacterium tuberculosis IspC by photometric assay	B	6.64	pIC50	230	nM	IC50	J Med Chem (2013) 56: 8151-8162 [PMID:24032981]
ChEMBL	Inhibition of Mycobacterium tuberculosis DXR assessed as NADPH-dependent conversion of 1-deoxy-D-xylulose 5-phosphate to 2-C-methyl-D-erythritol 4-phosphate by spectrophotometric method	B	7.1	pIC50	80	nM	IC50	J Biol Chem (2007) 282: 19905-19916 [PMID:17491006]
ChEMBL	Inhibition of Mycobacterium tuberculosis recombinant DXR expressed in Escherichia coli BL21 (DE3) using DXP as substrate and Mn2+ as cofactor preincubated for 10 mins	B	7.1	pIC50	80	nM	IC50	J Med Chem (2011) 54: 4721-4734 [PMID:21561155]
ChEMBL	Inhibition of Mycobacterium tuberculosis DXR assessed as reduction of 1-deoxy-D-xylulose 5-phosphate into 2-C-methyl-D-erythritol-4-phosphate measured for every 5 secs upto 500 secs by spectrophotometry analysis	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2011) 21: 5403-5407 [PMID:21824775]
ChEMBL	Inhibition of Mycobacterium tuberculosis DXR using DXP as substrate assessed as formation of MEP measured every 5 secs for 180 secs by spectrophotometric analysis	B	7.1	pIC50	80	nM	IC50	J Med Chem (2013) 56: 6190-6199 [PMID:23819803]
1-deoxy-D-xylulose 5-phosphate reductoisomerase in Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2010623] [UniProtKB: A0QVH7]
ChEMBL	Inhibition of Mycobacterium smegmatis DSM 43756 ATCC 19420 N-terminal his-tagged DXR expressed in XL1-blue Escherichia coli using NADPH and DXP as substrate preincubated for 2 mins with substrate before compound addition	B	6.29	pIC50	510	nM	IC50	Eur J Med Chem (2012) 51: 277-285 [PMID:22405649]
ChEMBL	Inhibition of Mycobacterium smegmatis DXR	B	7.1	pIC50	80	nM	IC50	Eur J Med Chem (2012) 51: 277-285 [PMID:22405649]
1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic in Arabidopsis thaliana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2285354] [UniProtKB: Q9XFS9]
ChEMBL	Inhibition of Arabidopsis thaliana IspC expressed in Escherichia coli after 40 min by spectrophotometric analysis	B	7	pIC50	100	nM	IC50	Pest Manag Sci (2013) 69: 559-563 [PMID:23471898]
1-deoxyxylulose-5-phosphate reductoisomerase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4091] [UniProtKB: P45568]
ChEMBL	Inhibition of recombinant Escherichia coli DXR	B	7.32	pIC50	48	nM	IC50	J Med Chem (2006) 49: 2656-2660 [PMID:16610809]
ChEMBL	Inhibition of His-tagged Escherichia coli DXR pre-incubated for 2 mins before reaction initiation in presence of 160 uM NADPH in absence of 0.01% Triton X100	B	7.49	pIC50	32	nM	IC50	Bioorg Med Chem (2014) 22: 3713-3719 [PMID:24890653]
ChEMBL	Inhibition of Escherichia coli Dxr	B	7.49	pIC50	32	nM	IC50	J Med Chem (2014) 57: 9740-9763 [PMID:25210872]
ChEMBL	Inhibition of recombinant Escherichia coli DXR	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem Lett (2006) 16: 1888-1891 [PMID:16439126]
ChEMBL	Inhibition of Escherichia coli DXR	B	7.52	pIC50	30	nM	IC50	ACS Med Chem Lett (2011) 2: 165-170 [PMID:21379374]
ChEMBL	Inhibitory concentration against DOXP reductoisomerase	B	7.7	pIC50	19.95	nM	IC50	J Med Chem (2005) 48: 3547-3563 [PMID:15887963]
1-deoxyxylulose-5-phosphate reductoisomerase in Synechocystis sp. (strain PCC 6803 / Kazusa) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4094] [UniProtKB: Q55663]
ChEMBL	Binding affinity against Synechocystis strain PCC6803 DXP reductoisomerase	B	7.68	pKi	21	nM	Ki	Bioorg Med Chem Lett (2004) 14: 5309-5312 [PMID:15454217]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antimicrobial activity against chloroquine-resistant Plasmodium falciparum K1 infected in human erythrocytes assessed as inhibition of parasite replication	F	5.43	pIC50	3700	nM	IC50	J Med Chem (2011) 54: 6796-6802 [PMID:21866890]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum K1 by micro dilution assay	F	5.76	pIC50	1730	nM	IC50	J Med Chem (2010) 53: 5342-5346 [PMID:20568776]
ChEMBL	Antiplasmodial activity against blood stage forms of chloroquine-sensitive Plasmodium falciparum D10 incubated for 3 days by HRP2 detection based ELISA method	F	5.89	pIC50	1300	nM	IC50	J Med Chem (2015) 58: 2025-2035 [PMID:25633870]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 in infected human erythrocytes after 48 hrs	F	5.92	pIC50	1200	nM	IC50	Bioorg Med Chem (2008) 16: 3361-3371 [PMID:18158249]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7	F	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2006) 16: 1888-1891 [PMID:16439126]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 assessed as parasite growth inhibition	F	6	pIC50	1000	nM	IC50	J Med Chem (2013) 56: 376-380 [PMID:23215035]
ChEMBL	Antimalarial activity against MRA-150, chloroquine, pyrimethamine, mefloquine-resistant asexual blood stage Plasmodium falciparum Dd2 after 72 hrs by SYBR green assay	F	6.06	pIC50	880	nM	IC50	Bioorg Med Chem Lett (2015) 25: 1515-1519 [PMID:25754494]
ChEMBL	Antiplasmodial activity against blood stage forms of chloroquine-sensitive Plasmodium falciparum 3D7 incubated for 3 days by HRP2 detection based ELISA method	F	6.06	pIC50	880	nM	IC50	J Med Chem (2015) 58: 2025-2035 [PMID:25633870]
ChEMBL	Antimalarial activity against chloroquine and mefloquine-resistant Plasmodium falciparum Dd2 after 48 hrs by SYBR Green I assay	F	6.09	pIC50	810	nM	IC50	Bioorg Med Chem Lett (2015) 25: 2112-2116 [PMID:25881827]
ChEMBL	Antiplasmodial activity against blood stage forms of multidrug-resistant Plasmodium falciparum Dd2 incubated for 3 days by HRP2 detection based ELISA method	F	6.09	pIC50	810	nM	IC50	J Med Chem (2015) 58: 2025-2035 [PMID:25633870]
ChEMBL	Inhibition of Plasmodium falciparum Dd2	F	6.32	pIC50	480	nM	IC50	J Med Chem (2006) 49: 2656-2660 [PMID:16610809]
ChEMBL	Inhibition of Plasmodium falciparum 3D7	F	6.4	pIC50	400	nM	IC50	J Med Chem (2006) 49: 2656-2660 [PMID:16610809]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2	F	6.44	pIC50	360	nM	IC50	Bioorg Med Chem Lett (2006) 16: 1888-1891 [PMID:16439126]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2 assessed as reduction in parasite viability by [3H]-hypoxanthine incorporation assay	F	6.54	pIC50	290	nM	IC50	J Med Chem (2016) 59: 5587-5603 [PMID:26791529]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 after 3 days by SYBR green I assay	F	5.93	pEC50	1170	nM	EC50	ACS Med Chem Lett (2013) 4: 278-282 [PMID:23795240]
ChEMBL	Antimalarial activity against Plasmodium falciparum	F	5.96	pEC50	1100	nM	EC50	ACS Med Chem Lett (2011) 2: 165-170 [PMID:21379374]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2 after 3 days by SYBR green I assay	F	6.36	pEC50	440	nM	EC50	ACS Med Chem Lett (2013) 4: 278-282 [PMID:23795240]
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum A2 [GtoPdb: 2980]
GtoPdb	Parasite growth inhibition assay	-	6.43	pIC50	370	nM	IC50	Science (1999) 285: 1573-6 [PMID:10477522]
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum Dd2 [GtoPdb: 2980]
GtoPdb	Parasite growth inhibition assay	-	6.54	pIC50	290	nM	IC50	Science (1999) 285: 1573-6 [PMID:10477522]
Plasmodium falciparum 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Plasmodium falciparum HB3 [GtoPdb: 2980]
GtoPdb	Parasite growth inhibition assay	-	6.46	pIC50	350	nM	IC50	Science (1999) 285: 1573-6 [PMID:10477522]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]