ChEMBL ligand: CHEMBL227744 (L-Nitroarginine, L-N-Nitroarginine, L-Nw-Nitroarginine)
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] Inducible NOS/Nitric oxide synthase, inducible in Rat [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Inhibition of nNOS assessed as conversion of L-[3H]arginine to L-[3H]citrulline	B	6.3	pKi	500	nM	Ki	Bioorg Med Chem (2008) 16: 6193-6206 [PMID:18477512]
GtoPdb	-	-	7.82	pKi	15	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme.	B	7.82	pKi	15	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of human nNOS by hemoglobin capture assay	B	5.36	pIC50	4400	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase	B	6.3	pIC50	500	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	Concentration required to inhibit neuronal nitric oxide synthase	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL	Inhibition of neuronal nitric oxide synthase (nNOS) in mice	B	6.3	pIC50	500	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of rat recombinant nNOS	B	5.34	pKi	4550	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of wild type rat nNOS by hemoglobin capture assay	B	5.22	pIC50	6000	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibition of recombinant rat nNOS expressed in Escherichia coli by hemoglobin capture assay	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL	Inhibition of rat nNOS expressed in HEK293T cells preincubated for 30 mins prior A23187-induced activation measured after 6 hrs by Griess assay	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem (2012) 20: 2435-2443 [PMID:22370337]
ChEMBL	Inhibition of rat nNOS L337H mutant by hemoglobin capture assay	B	5.4	pIC50	4000	nM	IC50	J Med Chem (2009) 52: 4533-4537 [PMID:19537690]
ChEMBL	Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay	B	6.22	pIC50	600	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons)	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
GtoPdb	-	-	7.52	pKi	30	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme.	B	7.52	pKi	30	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS)	B	6.3	pIC50	500	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against recombinant human Endothelial nitric oxide synthase	B	6.49	pIC50	320	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of endothelial nitric oxide synthase.	B	6.49	pIC50	320	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Inhibition of bovine recombinant eNOS	B	6.12	pKi	750	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of bovine recombinant endothelial NOS by hemoglobin capture assay	B	6.14	pKi	720	nM	Ki	J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL	Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells	B	5.92	pIC50	1200	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
GtoPdb	-	-	6.17	pKi	670	nM	Ki	J Med Chem (1998) 41: 2858-71 [PMID:9667974]
ChEMBL	Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme.	B	6.17	pKi	670	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
ChEMBL	Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS)	B	5.12	pIC50	7600	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory concentration against human Inducible nitric oxide synthase	B	5.26	pIC50	5500	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of inducible nitric oxide synthase.	B	5.26	pIC50	5500	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Inhibition of mouse recombinant inducible NOS by hemoglobin capture assay	B	5.37	pKi	4280	nM	Ki	J Med Chem (2007) 50: 2089-2099 [PMID:17425297]
ChEMBL	Inhibition of mouse recombinant iNOS	B	6.24	pKi	570	nM	Ki	J Med Chem (2010) 53: 7804-7824 [PMID:20958055]
ChEMBL	Inhibition of inducible nitric oxide synthase (iNOS) in mice	B	4.1	pIC50	80000	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
ChEMBL	Inhibition of iNOS in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction incubated for 24 hrs by Griess assay	B	4.56	pIC50	27550	nM	IC50	Bioorg Med Chem (2020) 28: 115733-115733 [PMID:33065432]
ChEMBL	Inhibition of inducible NOS mediated nitric oxide production in LPS-activated Kunming mouse macrophages	B	4.83	pIC50	14740	nM	IC50	Bioorg Med Chem (2008) 16: 578-585 [PMID:17937988]
ChEMBL	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	B	4.85	pIC50	14000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Tested for inhibition of mouse inducible nitric oxide synthase	B	6.3	pIC50	500	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]
Inducible NOS/Nitric oxide synthase, inducible in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
ChEMBL	Tested for inhibition of rat brain inducible nitric oxide synthase	B	4.7	pIC50	20000	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]