ChEMBL ligand: CHEMBL502 (D-797, Donaz, Donepezil, Donepezilo)
phosphodiesterase 4D/3`,5`-cyclic-AMP phosphodiesterase 4D in Human [ChEMBL: CHEMBL288] [GtoPdb: 1303] [UniProtKB: Q08499] There should be some charts here, you may need to enable JavaScript!
nicotinic acetylcholine receptor ε subunit/Acetylcholine receptor subunit epsilon in Human [ChEMBL: CHEMBL2484] [GtoPdb: 477] [UniProtKB: Q04844] There should be some charts here, you may need to enable JavaScript!
Acetylcholinesterase in Torpedo californica [ChEMBL: CHEMBL4780] [UniProtKB: P04058] Acetylcholinesterase in Electrophorus electricus [ChEMBL: CHEMBL4078] [UniProtKB: O42275] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303] Acetylcholinesterase in Bovine [ChEMBL: CHEMBL4768] [UniProtKB: P23795] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Mouse [ChEMBL: CHEMBL3198] [GtoPdb: 2465] [UniProtKB: P21836] acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Rat [ChEMBL: CHEMBL3199] [GtoPdb: 2465] [UniProtKB: P37136] There should be some charts here, you may need to enable JavaScript!
Monoamine oxidase B/Amine oxidase [flavin-containing] B in Human [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338] Monoamine oxidase B/Amine oxidase [flavin-containing] B in Rat [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643] There should be some charts here, you may need to enable JavaScript!
Amyloid-beta precursor protein in Human [ChEMBL: CHEMBL2487] [UniProtKB: P05067] There should be some charts here, you may need to enable JavaScript!
ABCB1/ATP-dependent translocase ABCB1 in Human [ChEMBL: CHEMBL4302] [GtoPdb: 768] [UniProtKB: P08183] There should be some charts here, you may need to enable JavaScript!
beta-secretase 1/Beta-secretase 1 in Human [ChEMBL: CHEMBL4822] [GtoPdb: 2330] [UniProtKB: P56817] There should be some charts here, you may need to enable JavaScript!
Butyrylcholinesterase in Equus caballus [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9] Butyrylcholinesterase in Rat [ChEMBL: CHEMBL3403] [UniProtKB: Q9JKC1] There should be some charts here, you may need to enable JavaScript!
phosphodiesterase 5A/cGMP-specific 3`,5`-cyclic phosphodiesterase in Human [ChEMBL: CHEMBL1827] [GtoPdb: 1304] [UniProtKB: O76074] There should be some charts here, you may need to enable JavaScript!
choline O-acetyltransferase/Choline O-acetyltransferase in Rat [ChEMBL: CHEMBL3945] [GtoPdb: 2480] [UniProtKB: P32738] There should be some charts here, you may need to enable JavaScript!
butyrylcholinesterase/Cholinesterase in Human [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276] Cholinesterase in Equus caballus [ChEMBL: CHEMBL5763] [UniProtKB: P81908] butyrylcholinesterase/Cholinesterase in Mouse [ChEMBL: CHEMBL2528] [GtoPdb: 2471] [UniProtKB: Q03311] There should be some charts here, you may need to enable JavaScript!
glycogen synthase kinase 3 beta/Glycogen synthase kinase-3 beta in Human [ChEMBL: CHEMBL262] [GtoPdb: 2030] [UniProtKB: P49841] There should be some charts here, you may need to enable JavaScript!
H3 receptor/Histamine H3 receptor in Human [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1] There should be some charts here, you may need to enable JavaScript!
carboxylesterase 1/Liver carboxylesterase 1 in Human [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141] There should be some charts here, you may need to enable JavaScript!
microtubule affinity regulating kinase 4/MAP/microtubule affinity-regulating kinase 4 in Human [ChEMBL: CHEMBL5754] [GtoPdb: 2100] [UniProtKB: Q96L34] There should be some charts here, you may need to enable JavaScript!
sigma non-opioid intracellular receptor 1/Sigma non-opioid intracellular receptor 1 in Human [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720] Sigma non-opioid intracellular receptor 1 in Guinea pig [ChEMBL: CHEMBL4153] [UniProtKB: Q60492] There should be some charts here, you may need to enable JavaScript!
Kv11.1/Voltage-gated inwardly rectifying potassium channel KCNH2 in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
phosphodiesterase 4D/3`,5`-cyclic-AMP phosphodiesterase 4D in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL288] [GtoPdb: 1303] [UniProtKB: Q08499]
ChEMBL	Inhibition of PDE4D7 (unknown origin)	B	4.44	pIC50	36000	nM	IC50	Eur J Med Chem (2022) 238: 114503-114503 [PMID:35689855]
nicotinic acetylcholine receptor ε subunit/Acetylcholine receptor subunit epsilon in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2484] [GtoPdb: 477] [UniProtKB: Q04844]
ChEMBL	Inhibition of human AChE by Ellman's method	B	7.49	pIC50	32.36	nM	IC50	Eur J Med Chem (2022) 230: 114098-114098 [PMID:35026532]
Acetylcholinesterase in Torpedo californica (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4780] [UniProtKB: P04058]
ChEMBL	In vitro inhibition of Torpedo californica acetylcholinesterase.	B	7.32	pIC50	48	nM	IC50	J Med Chem (1999) 42: 730-741 [PMID:10052979]
ChEMBL	Inhibition of Torpedo californica AChE (type VI-S) using acetylthiocholine iodide as substrate assessed as 5-thio-2-nitrobenzoate anion formation preincubated for 15 mins followed by substrate addition	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2015) 103: 361-369 [PMID:26363872]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase (AChE) in Torpedo californica	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem Lett (1998) 8: 575-580 [PMID:9871563]
Acetylcholinesterase in Electrophorus electricus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4078] [UniProtKB: O42275]
ChEMBL	Non competitive inhibition of electric eel AChE using acetylthiocholine iodide as substrate by Lineweaver-Burk plot analysis	B	7.25	pKi	56	nM	Ki	Bioorg Med Chem (2013) 21: 5451-5460 [PMID:23810675]
ChEMBL	Non-competitive inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate pretreated for 10 mins followed by substrate addition measured at 12 secs interval for 5 mins by Ellman's method	B	7.42	pKi	38	nM	Ki	Eur J Med Chem (2017) 130: 440-457 [PMID:28282613]
ChEMBL	Inhibition of electric eel AChE assessed as inhibition constant using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by Dixon plot analysis	B	7.79	pKi	16.4	nM	Ki	Eur J Med Chem (2019) 174: 116-129 [PMID:31029943]
ChEMBL	Inhibition of electric eel AChE pre-incubated for 3 mins before acetylthiocholine substrate addition by Lineweaver-Burk plot	B	8.37	pKi	4.24	nM	Ki	Eur J Med Chem (2015) 97: 181-189 [PMID:25969170]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins by Ellman method	B	4.58	pIC50	26100	nM	IC50	Bioorg Med Chem (2015) 23: 2445-2457 [PMID:25868744]
ChEMBL	Inhibition of electrical eel AChE using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by DTNB reagent based Ellman's method	B	5.14	pIC50	7300	nM	IC50	Bioorg Med Chem (2023) 78: 117132-117132 [PMID:36542960]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	5.14	pIC50	7230	nM	IC50	Bioorg Med Chem (2014) 22: 6163-6173 [PMID:25257911]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by Ellman's method	B	5.49	pIC50	3210	nM	IC50	Eur J Med Chem (2024) 271: 116388-116388 [PMID:38614062]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins under dark condition by Ellman's method	B	5.55	pIC50	2800	nM	IC50	Bioorg Med Chem (2019) 27: 4030-4040 [PMID:31378596]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 15 to 20 mins by Ellman's method	B	6.32	pIC50	480	nM	IC50	Bioorg Med Chem Lett (2021) 52: 128306-128306 [PMID:34371131]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 15 mins by Ellman's method	B	6.6	pIC50	250	nM	IC50	Eur J Med Chem (2022) 229: 114044-114044 [PMID:34923430]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured for 5 mins by Ellmans method	B	6.66	pIC50	219.7	nM	IC50	Eur J Med Chem (2016) 124: 1026-1040 [PMID:27783974]
ChEMBL	Inhibition of Electrophorus electricus AChE by Ellman's method	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2009) 19: 5075-5077 [PMID:19643609]
ChEMBL	Inhibition of electric eel AchE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	6.92	pIC50	120	nM	IC50	Eur J Med Chem (2014) 76: 314-331 [PMID:24589487]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman method	B	6.96	pIC50	110	nM	IC50	Bioorg Med Chem (2015) 23: 911-923 [PMID:25678013]
ChEMBL	Inhibition of Electrophorus electricus brain AChE by Ellman's method	B	6.96	pIC50	110	nM	IC50	Bioorg Med Chem (2008) 16: 8011-8021 [PMID:18701305]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	6.96	pIC50	110	nM	IC50	Eur J Med Chem (2015) 94: 348-366 [PMID:25778991]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured for 45 mins by Ellmans microplate assay	B	7	pIC50	100	nM	IC50	Bioorg Med Chem (2018) 26: 1511-1522 [PMID:29429576]
ChEMBL	Inhibition of electric eel AChE using ACTI as substrate preincubated for 29 mins followed by substrate addition measured at 1 min intervals for 10 mins by Ellman's assay	B	7.02	pIC50	96	nM	IC50	J Med Chem (2016) 59: 7683-7689 [PMID:27405689]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate pretreated for 10 mins followed by substrate addition measured after 20 mins by Ellman's method	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem (2018) 26: 3696-3706 [PMID:29886083]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate after 30 mins by Ellman's method	B	7.05	pIC50	90	nM	IC50	Medchemcomm (2012) 3: 1098-1103
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate by Ellman's method	B	7.1	pIC50	80	nM	IC50	Eur J Med Chem (2019) 179: 680-693 [PMID:31280020]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition measured for 5 mins by UV spectrophotometric analysis	B	7.17	pIC50	67	nM	IC50	Eur J Med Chem (2017) 139: 612-632 [PMID:28841514]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured every min for 10 mins by DTNB based Ellman's assay	B	7.18	pIC50	66	nM	IC50	Bioorg Med Chem (2024) 101: 117649-117649 [PMID:38401458]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	7.21	pIC50	62	nM	IC50	Eur J Med Chem (2021) 212: 113124-113124 [PMID:33395623]
ChEMBL	Inhibition of electric eel AChE using thiocholine iodide as substrate by Ellman's method	B	7.22	pIC50	59.9	nM	IC50	Bioorg Med Chem Lett (2015) 25: 5212-5216 [PMID:26454504]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured for 10 mins by modified Ellman's method	B	7.24	pIC50	57	nM	IC50	Bioorg Med Chem (2016) 24: 2352-2359 [PMID:27083471]
ChEMBL	Inhibition of electric eel AChE using ATCI and BTCI as substrate preincubated for 5 mins followed by substrate addition and measured after 3 mins by DTNB-reagent based Ellman's method	B	7.28	pIC50	52	nM	IC50	Bioorg Med Chem (2023) 78: 117146-117146 [PMID:36580744]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 3 mins by Ellman's method	B	7.29	pIC50	51	nM	IC50	Eur J Med Chem (2016) 123: 282-297 [PMID:27484514]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate incubated for 5 mins followed by substrate addition measured after 180 secs by Ellman's method	B	7.3	pIC50	50	nM	IC50	Eur J Med Chem (2018) 157: 161-176 [PMID:30096650]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine as substrate preincubated for 8 mins followed by substrate addition by Ellman's method	B	7.3	pIC50	50	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5573-5579 [PMID:27769620]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate by Ellman's method	B	7.3	pIC50	50	nM	IC50	Eur J Med Chem (2017) 127: 671-690 [PMID:27823887]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 2 mins followed by substrate addition and measured after 2 mins by Ellman's method	B	7.3	pIC50	50	nM	IC50	Bioorg Med Chem (2018) 26: 3812-3824 [PMID:29960728]
ChEMBL	Inhibition of electric eel Ache using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman method	B	7.31	pIC50	49	nM	IC50	Bioorg Med Chem (2019) 27: 3156-3166 [PMID:31176571]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured every minute for 10 mins by DTNB-reagent based Ellman's method	B	7.31	pIC50	49	nM	IC50	Eur J Med Chem (2020) 207: 112761-112761 [PMID:32942070]
ChEMBL	Inhibition of Electrophorus electricus AchE	B	7.31	pIC50	49	nM	IC50	Eur J Med Chem (2021) 221: 113492-113492 [PMID:33984802]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	7.31	pIC50	49	nM	IC50	Eur J Med Chem (2019) 175: 2-19 [PMID:31055149]
ChEMBL	Inhibition of electric eel AChE using DTNB as substrate incubated for 5 mins prior to substrate addition measured after 2 mins by Ellman's method	B	7.31	pIC50	49	nM	IC50	Bioorg Med Chem (2015) 23: 6389-6403 [PMID:26337018]
ChEMBL	In vitro inhibition of electric eel acetylcholinesterase	B	7.32	pIC50	48	nM	IC50	J Med Chem (2001) 44: 2707-2718 [PMID:11495583]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 5 mins by Ellman's method	B	7.32	pIC50	48	nM	IC50	Bioorg Med Chem (2015) 23: 1135-1148 [PMID:25624107]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by Ellman's method	B	7.32	pIC50	47.4	nM	IC50	Eur J Med Chem (2017) 139: 48-59 [PMID:28797883]
ChEMBL	Inhibition of Electrophorus electricus AchE using acetylcholine iodide as substrate incubated for 10 mins followed by substrate addition measured at 0 to 180 secs by Ellman's method	B	7.33	pIC50	47	nM	IC50	Eur J Med Chem (2022) 234: 114210-114210 [PMID:35303485]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 2 mins by spectroscopy based Ellman's method	B	7.33	pIC50	47	nM	IC50	Eur J Med Chem (2017) 136: 36-51 [PMID:28478343]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 10 mins by Ellman's method	B	7.35	pIC50	45	nM	IC50	Eur J Med Chem (2022) 238: 114503-114503 [PMID:35689855]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins by Ellman's method	B	7.38	pIC50	42	nM	IC50	Bioorg Med Chem Lett (2015) 25: 5576-5582 [PMID:26514744]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 5 mins prior to substrate addition measured after 5 mins by Ellman's method	B	7.39	pIC50	41.1	nM	IC50	Eur J Med Chem (2020) 185: 111785-111785 [PMID:31669851]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by DTNB reagent based Ellman's method	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2022) 242: 114630-114630 [PMID:35987018]
ChEMBL	Inhibition of electric eel acetylcholinesterase using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition by Ellman's method	B	7.39	pIC50	41	nM	IC50	Bioorg Med Chem (2014) 22: 1355-1361 [PMID:24461494]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins prior to substrate addition by Ellman's method	B	7.39	pIC50	40.74	nM	IC50	Bioorg Med Chem (2013) 21: 5451-5460 [PMID:23810675]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate pretreated for 6 mins followed by substrate addition measured up to 180 sec by Ellman's method	B	7.4	pIC50	40.2	nM	IC50	Eur J Med Chem (2017) 133: 184-196 [PMID:28388521]
ChEMBL	Inhibition of electric eel AChE	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2022) 61: 128602-128602 [PMID:35124202]
ChEMBL	Inhibition of electric eel ACHE preincubated for 6 mins followed by addition of acetylcholine iodide as substrate by Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2016) 24: 4324-4338 [PMID:27460699]
ChEMBL	Inhibition of electric eel AChE	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2023) 80: 117178-117178 [PMID:36706609]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2022) 229: 114095-114095 [PMID:34995924]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by spectrophotometric-based Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2016) 24: 1528-1539 [PMID:26917219]
ChEMBL	Inhibition of electric eel AChE using acetylcholine iodide as substrate incubated for 20 mins prior to substrate addition by Ellman method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2013) 23: 2984-2989 [PMID:23562059]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins prior to substrate addition by Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2013) 21: 5451-5460 [PMID:23810675]
ChEMBL	Inhibition of Electric eel AChE using acetylthiocholine iodide as substrate after 10 mins measured for every mins by Ellman's method	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2019) 163: 116-135 [PMID:30503937]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2017) 25: 1471-1480 [PMID:28126439]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodine as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by DTNB reagent based Ellman's method	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2022) 242: 114689-114689 [PMID:36007469]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	7.41	pIC50	39	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127477-127477 [PMID:32781220]
ChEMBL	Inhibition of Electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 2 mins by Ellman's method	B	7.41	pIC50	39	nM	IC50	Eur J Med Chem (2017) 125: 736-750 [PMID:27721157]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 2 mins by Ellman's method	B	7.41	pIC50	39	nM	IC50	Bioorg Med Chem (2016) 24: 3829-3841 [PMID:27353888]
ChEMBL	Inhibition of electric eel AChE preincubated for 15 mins by Ellman's method	B	7.42	pIC50	38.2	nM	IC50	Eur J Med Chem (2014) 87: 429-439 [PMID:25282266]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	7.42	pIC50	38	nM	IC50	Eur J Med Chem (2016) 107: 219-232 [PMID:26588065]
ChEMBL	Inhibition of electric eel AchE using acetylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition and measured after 2 mins by Ellman's method	B	7.42	pIC50	38	nM	IC50	Bioorg Med Chem (2016) 24: 2777-2788 [PMID:27157006]
ChEMBL	Inhibition of electric eel AChE preincubated for 5 mins followed by addition of acetylthiocholine iodide as substrate measured after 2 mins by Ellman's method	B	7.42	pIC50	38	nM	IC50	Eur J Med Chem (2016) 119: 260-277 [PMID:27227482]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition measured every 1 min by Ellman's method	B	7.42	pIC50	38	nM	IC50	J Med Chem (2013) 56: 9089-9099 [PMID:24160297]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition by spectrophotometric analysis	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem (2017) 25: 2946-2955 [PMID:28454848]
ChEMBL	Inhibition of electric eel acetylcholinesterase preincubated for 5 mins followed by addition of acetylthiocholine iodide as substrate measured after 60 to 120 mins by Ellmans method	B	7.46	pIC50	35	nM	IC50	Medchemcomm (2016) 7: 990-998
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 10 mins prior to substrate challenge measured after 15 mins by Ellman's method	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem (2014) 22: 2496-2507 [PMID:24657052]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 6 mins followed by substrate addition and measured for 180 secs by Ellman's method	B	7.49	pIC50	32	nM	IC50	RSC Med Chem (2020) 11: 225-233 [PMID:33479629]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured every 1 min for 20 mins by spectrophotometric analysis	B	7.51	pIC50	31	nM	IC50	Eur J Med Chem (2023) 259: 115670-115670 [PMID:37515920]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate measured for 6 mins by Ellman's method	B	7.51	pIC50	31	nM	IC50	Bioorg Med Chem (2017) 25: 3980-3988 [PMID:28587871]
ChEMBL	Inhibition of electric eel acetylcholinesterase using acetylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2014) 78: 167-177 [PMID:24681981]
ChEMBL	Inhibition of electric eel acetylcholinesterase using acetylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2014) 78: 43-53 [PMID:24675179]
ChEMBL	Inhibition of Electrophorus electricus AchE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric based Ellman's method	B	7.52	pIC50	30	nM	IC50	Bioorg Med Chem (2022) 71: 116952-116952 [PMID:35930852]
ChEMBL	Inhibition of electric eel AChE assessed as reduction in production of H202 using acetylcholine as substrate incubated for 15 mins followed by substrate addition by horse radish peroxidase/Amplex red reagent based fluorescence assay	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2019) 177: 221-234 [PMID:31151057]
ChEMBL	Inhibition of electric eel AChE	B	7.54	pIC50	29	nM	IC50	Eur J Med Chem (2020) 186: 111893-111893 [PMID:31761383]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by DTNB reagent based Ellman's method	B	7.54	pIC50	29	nM	IC50	Eur J Med Chem (2023) 257: 115502-115502 [PMID:37224761]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins by Ellman's method	B	7.54	pIC50	29	nM	IC50	Bioorg Med Chem Lett (2020) 30: 126900-126900 [PMID:31882295]
ChEMBL	Inhibition of electric eel AChE preincubated for 15 mins followed by acetylthiocholine iodide substrate addition measured for 5 mins by Ellman's spectrophotometric method	B	7.54	pIC50	28.7	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5387-5394 [PMID:27789142]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.56	pIC50	27.4	nM	IC50	Eur J Med Chem (2017) 126: 762-775 [PMID:27951485]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate by Ellman's method	B	7.57	pIC50	27	nM	IC50	Eur J Med Chem (2021) 216: 113310-113310 [PMID:33667847]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.57	pIC50	27	nM	IC50	Eur J Med Chem (2017) 135: 307-323 [PMID:28458136]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylcholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.58	pIC50	26.4	nM	IC50	Bioorg Med Chem (2020) 28: 115400-115400 [PMID:32146060]
ChEMBL	Inhibition of electric eel acetylcholine esterase using acetylcholine chloride as substrate incubated for 15 mins prior to substrate addition measured every 1 min by Ellman's assay	B	7.59	pIC50	26	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4462-4466 [PMID:22633691]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by Ellman's method	B	7.59	pIC50	26	nM	IC50	Eur J Med Chem (2018) 147: 48-65 [PMID:29421570]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate pretreated for 10 mins followed by substrate addition measured at 12 secs interval for 5 mins by Ellman's method	B	7.59	pIC50	26	nM	IC50	Eur J Med Chem (2017) 130: 440-457 [PMID:28282613]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	7.59	pIC50	26	nM	IC50	Eur J Med Chem (2021) 223: 113735-113735 [PMID:34371367]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.6	pIC50	25	nM	IC50	Bioorg Med Chem (2021) 35: 116074-116074 [PMID:33640707]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition measured every minute for 5 mins by Ellman's method	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2019) 174: 116-129 [PMID:31029943]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition by Ellman's method	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2019) 181: 111598-111598 [PMID:31415981]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate by Ellman's method	B	7.62	pIC50	24	nM	IC50	Eur J Med Chem (2019) 161: 292-309 [PMID:30366255]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured for 5 mins by Ellman's method	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2018) 26: 4952-4962 [PMID:30190181]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2018) 26: 1885-1895 [PMID:29500132]
ChEMBL	Inhibition of electric eel AChE pre-incubated for 5 mins before acetylthiocholine iodide substrate addition and measured after 15 mins by Ellman's method	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2019) 27: 2914-2922 [PMID:31128990]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2019) 27: 895-930 [PMID:30744931]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.65	pIC50	22.6	nM	IC50	Bioorg Med Chem (2017) 25: 1997-2009 [PMID:28237559]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.65	pIC50	22.6	nM	IC50	Bioorg Med Chem (2017) 25: 714-726 [PMID:27923535]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured for 1 min by DTNB reagent-based spectrophotometric analysis	B	7.66	pIC50	22	nM	IC50	Eur J Med Chem (2019) 182: 111613-111613 [PMID:31437780]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate by spectrophotometer analysis	B	7.66	pIC50	22	nM	IC50	Eur J Med Chem (2014) 82: 536-544 [PMID:24941128]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured for 20 mins at 1 min interval by Ellman's method	B	7.68	pIC50	21.08	nM	IC50	Eur J Med Chem (2018) 150: 87-101 [PMID:29524731]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with compounds for 20 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2023) 259: 115695-115695 [PMID:37567058]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.68	pIC50	21	nM	IC50	Bioorg Med Chem (2018) 26: 6115-6127 [PMID:30470598]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured upto 5 mins by Ellman's method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2019) 177: 414-424 [PMID:31158754]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured for 5 mins by spectrophotometric Ellman's method	B	7.68	pIC50	21	nM	IC50	J Med Chem (2016) 59: 6791-6806 [PMID:27347731]
ChEMBL	Inhibition of electric eel recombinant AChE preincubated for 1 min in presence of DNTB followed by addition of acetylthiocholine iodide substrate and measured after 20 mins by Ellman's method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2021) 223: 113663-113663 [PMID:34198150]
ChEMBL	Inhibition of electric eel AChE using ATC and BTC iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 2 mins by DTNB reagent based assay	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2022) 244: 114806-114806 [PMID:36223681]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB-reagent based Ellman's method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2024) 270: 116353-116353 [PMID:38579622]
ChEMBL	Inhibition of electric eel AChE assessed as residual enzyme activity using acetylthiocholine iodide as substrate and measured for 10 mins by Ellman's colorimetric assay	B	7.68	pIC50	21	nM	IC50	Bioorg Med Chem (2024) 110: 117829-117829 [PMID:39002183]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins before substrate addition measured for 10 mins by Ellman's method	B	7.69	pIC50	20.3	nM	IC50	Medchemcomm (2012) 3: 627-634
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by DTNB reagent based Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2023) 250: 115169-115169 [PMID:36753881]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition by Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2018) 152: 600-614 [PMID:29763808]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride by spectrophotometry	B	7.7	pIC50	20	nM	IC50	Medchemcomm (2013) 4: 925-931
ChEMBL	Inhibition of electric eel AChE preincubated for 15 mins followed by addition of acetylthiocholine iodide substrate by Ellman's method-based spectrophotometric analysis	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2380-2382 [PMID:27017111]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured for 5 mins by spectrophotometry based Ellman method	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2017) 27: 1179-1185 [PMID:28189420]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 30 min by DTNB reagent based Ellman's method	B	7.7	pIC50	20	nM	IC50	RSC Med Chem (2024) 15: 2922-2936 [PMID:39149109]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2024) 266: 116131-116131 [PMID:38215587]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition by Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2022) 244: 114841-114841 [PMID:36257284]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate pre-incubated for 30 mins followed by substrate addition and measured after 5 mins by DTNB reagent based Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2024) 264: 116013-116013 [PMID:38052155]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2020) 192: 112180-112180 [PMID:32131034]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide incubated for 15 mins as substrate by Ellman's method	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem Lett (2019) 29: 126625-126625 [PMID:31444085]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate after 15 mins by Ellman's method	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem (2017) 25: 3006-3017 [PMID:28487125]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine as substrate incubated for 15 mins by Ellman's method	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5046-5052 [PMID:29033233]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase (AChE) in electric eel	B	7.77	pIC50	17	nM	IC50	Bioorg Med Chem Lett (1998) 8: 575-580 [PMID:9871563]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine as substrate by Ellman's method	B	7.77	pIC50	17	nM	IC50	Eur J Med Chem (2024) 276: 116592-116592 [PMID:39013357]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured at 1 to 3 mins time interval by Ellman's method	B	7.79	pIC50	16.3	nM	IC50	Medchemcomm (2017) 8: 1459-1467 [PMID:30108857]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate measured for 2 mins by Ellman's method	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2016) 121: 40-46 [PMID:27214510]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 5 mins by DTNB-reagent based Ellman's method	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2022) 227: 113973-113973 [PMID:34752955]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2022) 230: 114098-114098 [PMID:35026532]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem (2019) 27: 991-1001 [PMID:30772129]
ChEMBL	Inhibition of Electrophorus electricus acetylcholinesterase using acetylthiocholine iodide substrate after 15 mins by spectrophotometric method	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2035-2039 [PMID:26947607]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	7.83	pIC50	14.79	nM	IC50	Eur J Med Chem (2022) 230: 114098-114098 [PMID:35026532]
ChEMBL	Inhibition of electric eel AChE using acetylcholine as substrate preincubated for 15 mins prior to substrate addition by Ellman's method	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2013) 70: 623-630 [PMID:24211638]
ChEMBL	Inhibition of electric eel AChE incubated for 15 mins using acetylcholine iodide substrate by colorimetric Ellman's method	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2015) 94: 195-210 [PMID:25768702]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide substrate by Ellman method based spectrophotometry	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2014) 84: 375-381 [PMID:25036795]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 2 mins by Ellman's method	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2013) 68: 260-269 [PMID:23988409]
ChEMBL	Inhibition of electric eel AChE by Ellman's method	B	7.87	pIC50	13.4	nM	IC50	J Med Chem (2010) 53: 5129-5143 [PMID:20575555]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine chloride as substrate by Ellman's method	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2023) 251: 115253-115253 [PMID:36921526]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 5 mins by Ellman's method	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2014) 75: 82-95 [PMID:24530494]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 2 mins by Ellman's method	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2018) 158: 353-370 [PMID:30223122]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	RSC Med Chem (2024) 15: 2037-2044 [PMID:38911155]
ChEMBL	Inhibition of Electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2017) 125: 676-695 [PMID:27721153]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2021) 218: 113397-113397 [PMID:33838585]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition and measured after 5 mins by spectrophotometric based Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2021) 225: 113783-113783 [PMID:34461507]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by DTNB reagent based Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2023) 261: 115832-115832 [PMID:37837674]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2020) 187: 111916-111916 [PMID:31812794]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by DTNB-reagent based Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2023) 249: 115135-115135 [PMID:36696766]
ChEMBL	Inhibition of Electrophorus electricus AChE pre-incubated for 5 mins before addition of acetylthiocholine iodide substrate by Ellman's assay	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2015) 23: 1629-1637 [PMID:25707322]
ChEMBL	Inhibition of Electrophorus electricus AChE after 5 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2015) 92: 738-749 [PMID:25621991]
ChEMBL	Inhibition of electric eel AChE preincubated for 5 mins followed by acetylthiocholine iodide substrate addition measured after 5 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2016) 124: 63-81 [PMID:27560283]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellmans method	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4140-4145 [PMID:27445168]
ChEMBL	Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 4 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2021) 32: 115991-115991 [PMID:33440318]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 180 secs by spectrophotometric analysis	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2017) 139: 68-83 [PMID:28800459]
ChEMBL	Inhibition of acetylcholinesterase (AChE) in electric eel (type V-S) by modified radiometric assay	B	8.04	pIC50	9.2	nM	IC50	Bioorg Med Chem Lett (1992) 2: 861-864
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by Ellman's method	B	8.15	pIC50	7	nM	IC50	Eur J Med Chem (2017) 125: 853-864 [PMID:27744252]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	8.17	pIC50	6.7	nM	IC50	Eur J Med Chem (2012) 52: 251-262 [PMID:22503231]
ChEMBL	Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 5 mins by Ellman's method	B	8.17	pIC50	6.7	nM	IC50	Eur J Med Chem (2014) 80: 543-561 [PMID:24813882]
ChEMBL	Inhibition of Electrophorus electricus AchE using acetylthiocholine iodide as substrate preincubated for 10 mins measured after 15 mins of substrate addition by Ellman's method	B	8.17	pIC50	6.7	nM	IC50	J Med Chem (2011) 54: 8251-8270 [PMID:22023459]
ChEMBL	Inhibition Assay: The inhibitory activity of the enzyme acetylcholinesterase (AChE) was evaluated by the Ellman method (Biochem. Pharmacol. 1961, 7, 88) using an electric eel as an AChE neuronal model (Electrophorus electricus) and acetylthiocholine iodide (0.35 mM) as substrate. The reaction took place in a final volume of 3 mL of a 0.1 M phosphate buffer solution, pH 8.0, containing 0.035 units of AChE and used a 0.35 mM solution of 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) to produce the 5-thio-2-nitrobenzoic acid anion. Inhibition curves were performed in triplicate by incubating at least nine inhibitor concentrations for 10 min. A triplicate sample without inhibitor was always present so as to be aware of 100% of the AChE activity. After this time, the substrate was added to 0.35 mM acetylthiocholine iodide, from a 10 mM stock solution. Loss of color was observed at 412 nm in a spectrophotometric reader having 96 well plates.	B	8.17	pIC50	6.7	nM	IC50	US-8999994-B2. Derivatives of propargylamine having neuroprotective capacity for the treatment of Alzheimer's and Parkinson's diseases (2015)
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL220] [GtoPdb: 2465] [UniProtKB: P22303]
ChEMBL	Binding affinity to CM5 sensor chip immobilized recombinant human AChE assessed as dissociation constant at 298.15 K by surface plasmon resonance assay	B	8.1	pKd	8	nM	Kd	J Med Chem (2021) 64: 1844-1855 [PMID:33570950]
ChEMBL	Mixed-type inhibition of human AChE using acetylthiocholine chloride as substrate incubated for 5 mins followed by substrate addition by Lineweaver-Burk plot analysis	B	7.42	pKi	38	nM	Ki	Eur J Med Chem (2018) 157: 161-176 [PMID:30096650]
ChEMBL	Inhibition of human recombinant AChE	B	7.69	pKi	20.5	nM	Ki	J Med Chem (2004) 47: 6490-6498 [PMID:15588084]
ChEMBL	Inhibition of AChE	B	7.69	pKi	20.5	nM	Ki	J Med Chem (2010) 53: 5906-5914 [PMID:20420456]
ChEMBL	Inhibitory potency was evaluated against recombinant human AChE	B	7.69	pKi	20.5	nM	Ki	J Med Chem (2003) 46: 2279-2282 [PMID:12773032]
ChEMBL	Mixed type inhibition of human AChE using acetylthiocholineiodide as substrate measured for every 30 sec for 5 mins by Ellman's method	B	7.9	pKi	12.7	nM	Ki	ACS Med Chem Lett (2020) 11: 869-876 [PMID:32435398]
ChEMBL	Mixed inhibition of human erythrocytes AchE using acetylthiocholine iodide as substrate incubated for 20 mins by Lineweaver-Burk plot analysis	B	8	pKi	10	nM	Ki	ACS Med Chem Lett (2016) 7: 470-475 [PMID:27190595]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate after 60 mins by Ellman's method	B	8.14	pKi	7.2	nM	Ki	Bioorg Med Chem Lett (2013) 23: 702-705 [PMID:23276831]
ChEMBL	Inhibition of AChE-induced amyloid beta (1-40) aggregation	B	4	pIC50	>100000	nM	IC50	J Med Chem (2010) 53: 5906-5914 [PMID:20420456]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate by UV-vis spectrophotometry based Ellman's method	B	5.12	pIC50	7560	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127370-127370 [PMID:32738978]
ChEMBL	Inhibition of recombinant human AChE using ATC as substrate by DTNB-reagent based Ellman's method	B	5.15	pIC50	7120	nM	IC50	J Med Chem (2020) 63: 9695-9704 [PMID:32787143]
ChEMBL	Inhibition of human plasma BChE	B	5.38	pIC50	4150	nM	IC50	Eur J Med Chem (2011) 46: 2170-2184 [PMID:21459491]
ChEMBL	Inhibition of human erythrocytes BChE	B	5.38	pIC50	4150	nM	IC50	Eur J Med Chem (2011) 46: 2170-2184 [PMID:21459491]
ChEMBL	Inhibition of human plasma AChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 10 mins by Ellman's method	B	5.66	pIC50	2170	nM	IC50	Eur J Med Chem (2020) 198: 112368-112368 [PMID:32388114]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate measured after 15 to 20 mins by DTNB reagent based Ellman's method	B	6.66	pIC50	220	nM	IC50	Eur J Med Chem (2023) 262: 115880-115880 [PMID:37871406]
ChEMBL	Inhibition of AChE	B	6.8	pIC50	160	nM	IC50	Bioorg Med Chem (2008) 16: 2968-2973 [PMID:18249544]
ChEMBL	Inhibition of human erythrocytic AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 180 secs by spectrophotometric analysis	B	6.8	pIC50	160	nM	IC50	Eur J Med Chem (2017) 139: 68-83 [PMID:28800459]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by DTNB reagent based Ellman's method	B	6.83	pIC50	147.91	nM	IC50	Bioorg Med Chem (2019) 27: 1327-1340 [PMID:30795991]
ChEMBL	Inhibition of human erythrocyte ACHE preincubated for 6 mins followed by addition of acetylcholine iodide as substrate by Ellman's method	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem (2016) 24: 4324-4338 [PMID:27460699]
ChEMBL	Inhibition of human acetyl cholinesterase by Ellmann's method	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2010) 20: 7064-7066 [PMID:20951037]
ChEMBL	Inhibition of acetylcholinesterase (unknown origin) using acetylthiocholine iodide as substrate incubated for 10 mins followed by substrate addition measured after 20 mins by UV-Vis spectrophotometric analysis	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem (2017) 25: 100-106 [PMID:27780618]
ChEMBL	Inhibition of human AChE by Ellmans test	B	7.05	pIC50	90	nM	IC50	J Med Chem (2010) 53: 6490-6505 [PMID:20684567]
ChEMBL	Inhibition of human acetylcholinesterase using acetylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.06	pIC50	87	nM	IC50	RSC Med Chem (2022) 13: 1644-1656 [PMID:36561075]
ChEMBL	Inhibition of AChE (unknown origin) by Ellman's method	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2024) 98: 129566-129566 [PMID:38008338]
ChEMBL	Inhibition of AChE (unknown origin) by Ellman's method	B	7.1	pIC50	79	nM	IC50	RSC Med Chem (2024) 15: 207-222 [PMID:38283217]
ChEMBL	Inhibition of AChE (unknown origin) after 5 mins by Ellman's method	B	7.21	pIC50	62	nM	IC50	Eur J Med Chem (2014) 83: 355-365 [PMID:24980117]
ChEMBL	Inhibition of AChE in human erythrocytes using acetylthiocholine iodide as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured after 30 mins by Ellman's method	B	7.22	pIC50	60	nM	IC50	Bioorg Med Chem (2021) 46: 116385-116385 [PMID:34481338]
ChEMBL	Inhibition of human recombinant AChe using acetylthiocholine iodide as substrate incubated for 10 mins by DTNB reagent based Ellman's method	B	7.22	pIC50	60	nM	IC50	J Med Chem (2022) 65: 12292-12318 [PMID:36084304]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate by Ellman's method	B	7.23	pIC50	59	nM	IC50	Eur J Med Chem (2019) 182: 111651-111651 [PMID:31479975]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate incubated for 5 mins by Ellman's method	B	7.23	pIC50	59	nM	IC50	Eur J Med Chem (2022) 227: 113964-113964 [PMID:34743062]
ChEMBL	Inhibition of C-terminal 6His-tagged human recombinant AChE (32 to 614 residues) expressed in HEK293 cells using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by Ellman's method	B	7.27	pIC50	54	nM	IC50	Eur J Med Chem (2022) 231: 114152-114152 [PMID:35101650]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine as substrate by Ellman's method	B	7.3	pIC50	50.12	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4180-4184 [PMID:28751142]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine chloride as substrate incubated for 5 mins followed by substrate addition measured after 180 secs by Ellman's method	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2018) 157: 161-176 [PMID:30096650]
ChEMBL	Inhibition of recombinant human AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2021) 212: 113124-113124 [PMID:33395623]
ChEMBL	Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 3 mins by Ellman's method	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2016) 123: 282-297 [PMID:27484514]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition by DTNB based Ellman's colorimetric method	B	7.33	pIC50	47	nM	IC50	Eur J Med Chem (2024) 271: 116409-116409 [PMID:38663285]
ChEMBL	Inhibition of human AChE using ATC and BTC iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 2 mins by DTNB reagent based assay	B	7.36	pIC50	44	nM	IC50	Eur J Med Chem (2022) 244: 114806-114806 [PMID:36223681]
ChEMBL	Inhibition of human recombinant AChE preincubated for 1 min in presence of DNTB followed by addition of acetylthiocholine iodide substrate and measured after 20 mins by Ellman's method	B	7.36	pIC50	44	nM	IC50	Eur J Med Chem (2021) 223: 113663-113663 [PMID:34198150]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by DTNB based Ellman's method	B	7.37	pIC50	43	nM	IC50	Eur J Med Chem (2024) 271: 116450-116450 [PMID:38701714]
ChEMBL	Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by Ellman's method	B	7.38	pIC50	42	nM	IC50	Eur J Med Chem (2019) 183: 111707-111707 [PMID:31561043]
ChEMBL	Inhibition of human AChE by Ellman's method	B	7.38	pIC50	42	nM	IC50	Bioorg Med Chem (2020) 28: 115721-115721 [PMID:33007563]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by Ellman's method	B	7.39	pIC50	41	nM	IC50	Eur J Med Chem (2018) 146: 287-298 [PMID:29407958]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 60 to 300 sec by Ellman's method	B	7.4	pIC50	40	nM	IC50	Medchemcomm (2015) 6: 1930-1941
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 6 mins by Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem (2019) 27: 3650-3662 [PMID:31288978]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate treated 5 mins before substrate addition measured up to 4 mins by Ellman's method	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2013) 23: 4336-4341 [PMID:23791077]
ChEMBL	Inhibitory concentration required against acetylcholinesterase activity	B	7.4	pIC50	40	nM	IC50	J Med Chem (2001) 44: 4011-4014 [PMID:11689088]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate pretreated for 6 mins followed by substrate addition measured up to 180 sec by Ellman's method	B	7.42	pIC50	37.6	nM	IC50	Eur J Med Chem (2017) 133: 184-196 [PMID:28388521]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.45	pIC50	35.6	nM	IC50	Eur J Med Chem (2017) 126: 762-775 [PMID:27951485]
ChEMBL	Inhibition of human erythrocyte acetylcholinesterase using acetylthiocholine iodide as substrate pre-incubated for 10 mins followed by substrate addition and measured for 6 mins by Ellman's method	B	7.48	pIC50	33	nM	IC50	Eur J Med Chem (2019) 167: 510-524 [PMID:30784883]
ChEMBL	Inhibition of human recombinant AchE	B	7.49	pIC50	32	nM	IC50	Eur J Med Chem (2021) 221: 113492-113492 [PMID:33984802]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate after 5 mins by DTNB method	B	7.49	pIC50	32	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4707-4712 [PMID:22704921]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	7.49	pIC50	32	nM	IC50	Bioorg Med Chem (2021) 35: 116074-116074 [PMID:33640707]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by Ellman's method	B	7.5	pIC50	31.8	nM	IC50	Eur J Med Chem (2017) 139: 48-59 [PMID:28797883]
ChEMBL	Inhibition of human erythrocytes acetylcholinesterase preincubated for 5 mins followed by addition of acetylthiocholine iodide as substrate measured after 60 to 120 mins by Ellmans method	B	7.51	pIC50	30.8	nM	IC50	Medchemcomm (2016) 7: 990-998
ChEMBL	Inhibition of AChE (unknown origin) preincubated for 30 mins followed by substrate addition acetylthiocholineiodide measured after 40 mins by Ellman's method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2020) 200: 112415-112415 [PMID:32454229]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine chloride as substrate pretreated for 15 mins followed by substrate addition measured for 2 mins by DTNB reagent based spectrophotometric method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2017) 130: 139-153 [PMID:28242549]
ChEMBL	Inhibition of recombinant human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by spectrophotometry based Ellman's method	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2020) 198: 112257-112257 [PMID:32375073]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine iodide as a substrate preincubated for 20 mins followed by substrate addition by spectrophotometric analysis	B	7.57	pIC50	26.8	nM	IC50	Eur J Med Chem (2021) 225: 113779-113779 [PMID:34418785]
ChEMBL	Inhibition of AChE in human erythrocytes using acetylthiocholineiodide as substrate pre-incubated for 10 mins followed by substrate addition and measured for 6 mins by DTNB reagent based Ellman's method	B	7.58	pIC50	26.55	nM	IC50	Eur J Med Chem (2021) 215: 113224-113224 [PMID:33582578]
ChEMBL	Inhibition of AChE (unknown origin) by Ellman's method	B	7.6	pIC50	25	nM	IC50	Bioorg Med Chem Lett (2022) 75: 128976-128976 [PMID:36067929]
ChEMBL	Inhibition of AChE (unknown origin)	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2024) 273: 116523-116523 [PMID:38795518]
ChEMBL	Inhibition of human AChE	B	7.6	pIC50	25	nM	IC50	Eur J Med Chem (2023) 259: 115670-115670 [PMID:37515920]
ChEMBL	Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured at 1 to 3 mins time interval by Ellman's method	B	7.61	pIC50	24.7	nM	IC50	Medchemcomm (2017) 8: 1459-1467 [PMID:30108857]
ChEMBL	Inhibition of human recombinant acetylcholinesterase	B	7.64	pIC50	23.1	nM	IC50	J Med Chem (2007) 50: 4882-4897 [PMID:17850125]
ChEMBL	Inhibitory potency was evaluated against recombinant human AChE	B	7.64	pIC50	23.1	nM	IC50	J Med Chem (2003) 46: 2279-2282 [PMID:12773032]
ChEMBL	Inhibition of human recombinant AChE by Ellman's assay	B	7.64	pIC50	23.1	nM	IC50	J Med Chem (2008) 51: 7308-7312 [PMID:18954037]
ChEMBL	Inhibition of human recombinant AChE by Ellman's spectrophotometric assay	B	7.64	pIC50	23	nM	IC50	J Med Chem (2022) 65: 3962-3977 [PMID:35195417]
ChEMBL	Inhibition of human AchE	B	7.64	pIC50	23	nM	IC50	J Med Chem (2008) 51: 347-372 [PMID:18181565]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate by Ellman's assay	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2011) 46: 1682-1693 [PMID:21397996]
ChEMBL	Inhibition of human recombinant AChE	B	7.64	pIC50	22.91	nM	IC50	J Med Chem (2004) 47: 6490-6498 [PMID:15588084]
ChEMBL	Anticholinesterase activity against human erythrocyte AChE	B	7.66	pIC50	22	nM	IC50	J Med Chem (2006) 49: 2174-2185 [PMID:16570913]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by Ellman's method	B	7.66	pIC50	22	nM	IC50	Bioorg Med Chem (2016) 24: 1050-1062 [PMID:26827140]
ChEMBL	Inhibition of AChE (unknown origin)	B	7.66	pIC50	22	nM	IC50	Eur J Med Chem (2023) 259: 115670-115670 [PMID:37515920]
ChEMBL	Inhibition of human plasma AChE	B	7.66	pIC50	22	nM	IC50	Eur J Med Chem (2011) 46: 2170-2184 [PMID:21459491]
ChEMBL	Inhibition of human erythrocytes AChE	B	7.66	pIC50	22	nM	IC50	Eur J Med Chem (2011) 46: 2170-2184 [PMID:21459491]
ChEMBL	Ex vivo inhibition of human erythrocyte Acetylcholinesterase.	B	7.66	pIC50	22	nM	IC50	J Med Chem (2002) 45: 3684-3691 [PMID:12166941]
ChEMBL	Inhibitory concentration against human erythrocyte Acetylcholinesterase	B	7.66	pIC50	22	nM	IC50	J Med Chem (2005) 48: 986-994 [PMID:15715468]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by DTNB reagent based Ellman's method	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2022) 242: 114630-114630 [PMID:35987018]
ChEMBL	Inhibition of acetylcholine esterase (unknown origin) using acetylthiocholine as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by spectrophotometry	B	7.68	pIC50	21	nM	IC50	Eur J Med Chem (2013) 63: 854-868 [PMID:23603045]
ChEMBL	Inhibition of human serum AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured for 3 mins by Ellman's method	B	7.69	pIC50	20.3	nM	IC50	Eur J Med Chem (2017) 141: 197-210 [PMID:29031067]
ChEMBL	Inhibition of human recombinant AChE by Ellman's method	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2009) 44: 1341-1348 [PMID:18396354]
ChEMBL	Inhibition of human recombinant AChE preincubated for 20 mins before substrate addition by Ellman's method	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem (2010) 18: 1749-1760 [PMID:20171894]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB-reagent based Ellman's method	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2024) 270: 116353-116353 [PMID:38579622]
ChEMBL	Inhibition of human recombinant AChE after 20 mins using acetylthiocholine iodide as a substrate by Ellman's assay	B	7.74	pIC50	18	nM	IC50	J Med Chem (2011) 54: 2627-2645 [PMID:21417225]
ChEMBL	In vitro inhibition of human recombinant AChE.	B	7.74	pIC50	18	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2565-2568 [PMID:12182861]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2021) 223: 113735-113735 [PMID:34371367]
ChEMBL	Inhibition of human erythrocytes AChE by Ellman's method	B	7.75	pIC50	17.76	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127659-127659 [PMID:33137375]
ChEMBL	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate by Ellman's method	B	7.77	pIC50	17	nM	IC50	Eur J Med Chem (2024) 274: 116511-116511 [PMID:38820854]
ChEMBL	Inhibition of human serum recombinant AChE using acetylthiocholine iodide as a substrate incubated for 20 mins by DTNB reagent based Ellman's method	B	7.77	pIC50	17	nM	IC50	RSC Med Chem (2022) 13: 873-883 [PMID:35923722]
ChEMBL	Inhibition of human AChE by Ellman's spectrophotometric method	B	7.77	pIC50	17	nM	IC50	Eur J Med Chem (2022) 237: 114358-114358 [PMID:35462163]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by DTNB reagent based Ellman's method	B	7.77	pIC50	17	nM	IC50	Eur J Med Chem (2023) 250: 115169-115169 [PMID:36753881]
ChEMBL	Inhibition of human AChE using acetylthiocholineiodide as substrate measured for every 30 sec for 5 mins by Ellman's method	B	7.79	pIC50	16.1	nM	IC50	ACS Med Chem Lett (2020) 11: 869-876 [PMID:32435398]
ChEMBL	Inhibition of recombinant human AChE expressed in HEK293 cells preincubated for 15 mins	B	7.8	pIC50	16	nM	IC50	J Med Chem (2014) 57: 10455-10463 [PMID:25418133]
ChEMBL	Inhibitory concentration against acetylcholinesterase (AChE) from human erythrocytes	B	7.8	pIC50	16	nM	IC50	J Med Chem (1999) 42: 730-741 [PMID:10052979]
ChEMBL	In vitro inhibition of acetylcholinesterase from human erythrocytes	B	7.8	pIC50	16	nM	IC50	J Med Chem (2001) 44: 2707-2718 [PMID:11495583]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured upto 5 mins by Ellman's method	B	7.8	pIC50	16	nM	IC50	Eur J Med Chem (2019) 177: 414-424 [PMID:31158754]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured for 5 mins by Ellman's spectrophotometric method	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem (2023) 84: 117256-117256 [PMID:37003157]
ChEMBL	Inhibition of human recombinant AChE by spectrophotometric Ellman's method	B	7.82	pIC50	15	nM	IC50	J Med Chem (2015) 58: 5561-5578 [PMID:26107513]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by Ellman's method	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2015) 103: 302-311 [PMID:26363866]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine as substrate measured after 5 mins by Ellman's method	B	7.85	pIC50	14	nM	IC50	Eur J Med Chem (2020) 191: 112140-112140 [PMID:32088494]
ChEMBL	Inhibition of human erythrocytes AChE assessed as reduction in 5-thio-2-nitrobenzoate anion formation using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by Ellman's method	B	7.92	pIC50	12.1	nM	IC50	Eur J Med Chem (2019) 182: 111596-111596 [PMID:31419776]
ChEMBL	Inhibition of AChE in human erythrocytes using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2015) 94: 348-366 [PMID:25778991]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2017) 135: 307-323 [PMID:28458136]
ChEMBL	Inhibition of human erythrocyte AChE preincubated for 15 mins by Ellman's method	B	7.93	pIC50	11.7	nM	IC50	Eur J Med Chem (2014) 87: 429-439 [PMID:25282266]
ChEMBL	Inhibition Assay: Acetylcholinesterase extracted from human erythrocytes (buffered aqueous solution, ≧500 units/mg, Sigma Aldrich) is diluted in a 20 mM HEPES buffer pH8, 0.1% Triton X-100, to obtain a standard solution with 2.5 units of enzyme activity/ml. 100 μL of a 0.3 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) solution in a pH 7.4 phosphate buffer are introduced into a 96-well plate, followed by 50 μL of compound to be tested in solution in DMSO and 50 μL of enzyme solution. After 5 min of preincubation, the reaction is initiated by injecting 50 μL of 10 mM acetylthiocholine iodide solution. Hydrolysis of the acetylthiocholine is followed by the formation of the anion 5-thio-2-nitrobenzoate, produced by the reaction of the DTNB with the thiocholine released by the enzymatic hydrolysis of the acetylthiocholine, by means of a microplate reader (TECAN Infinite M200, Lyon, France) at a wavelength of 412 nm per minute for 10 minutes. Donepezil is used as a reference product.	B	7.96	pIC50	11	nM	IC50	US-9663465-B2. Acetylcholinesterase inhibitors and promnesiant serotonin 5-HT4 receptor agonists, their methods of preparation and the pharmaceutical compositions containing the same (2017)
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition measured every 1 min by Ellman's method	B	7.96	pIC50	11	nM	IC50	J Med Chem (2013) 56: 9089-9099 [PMID:24160297]
ChEMBL	Inhibition of human erythrocyte acetylcholinesterase using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition by Ellman's method	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem (2014) 22: 1355-1361 [PMID:24461494]
ChEMBL	Inhibition of human erythrocytes AchE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2014) 76: 314-331 [PMID:24589487]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine chloride by spectrophotometry	B	7.96	pIC50	11	nM	IC50	Medchemcomm (2013) 4: 925-931
ChEMBL	Inhibition of human erythrocytes AchE using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman method	B	7.96	pIC50	11	nM	IC50	ACS Med Chem Lett (2016) 7: 470-475 [PMID:27190595]
ChEMBL	Inhibition of AChE in human erythrocytes using acetylthiocholine chloride as substrate measured after 15 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2017) 130: 379-392 [PMID:28279845]
ChEMBL	Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2020) 187: 111958-111958 [PMID:31865014]
ChEMBL	Inhibition of recombinant human AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition and measured for 5 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2018) 156: 534-553 [PMID:30025348]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2018) 158: 781-800 [PMID:30245401]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by spectrophotometric-based Ellman's method	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2016) 24: 1528-1539 [PMID:26917219]
ChEMBL	Inhibition of human AChE using acetylthiocholine as substrate by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2014) 73: 56-72 [PMID:24378710]
ChEMBL	Inhibition of AChE (unknown origin)	B	8	pIC50	10	nM	IC50	RSC Med Chem (2024) 15: 2037-2044 [PMID:38911155]
ChEMBL	Inhibition of purified human erythrocyte AChE using acetylcholine as substrate by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2013) 67: 64-74 [PMID:23838422]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine as substrate by Ellman method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2012) 57: 296-301 [PMID:23078965]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2016) 121: 376-386 [PMID:27267007]
ChEMBL	Inhibition of human erythrocyte AChE using ATCI as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman assay	B	8	pIC50	10	nM	IC50	J Med Chem (2016) 59: 5823-5846 [PMID:27253679]
ChEMBL	Inhibition of recombinant human AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by UV-Vis spectrophotometric method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2017) 130: 60-72 [PMID:28242552]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine as substrate by Ellman's assay	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2019) 166: 90-107 [PMID:30685536]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2024) 271: 116386-116386 [PMID:38614063]
ChEMBL	Inhibition of recombinant human AChE using acetylthiocholine iodide as substrate measured after 7 mins by Ellman's method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2021) 222: 113541-113541 [PMID:34116326]
ChEMBL	Inhibition of acetylcholinesterase (unknown origin)	B	8.05	pIC50	9	nM	IC50	J Med Chem (2020) 63: 15187-15217 [PMID:33111525]
ChEMBL	Inhibition of AChE in human erythrocytes by Ellman's method	B	8.05	pIC50	9	nM	IC50	Eur J Med Chem (2020) 192: 112180-112180 [PMID:32131034]
ChEMBL	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method	B	8.05	pIC50	9	nM	IC50	Eur J Med Chem (2016) 121: 864-879 [PMID:26471320]
ChEMBL	Inhibition of AChE in human erythrocytes by Ellman's method	B	8.09	pIC50	8.2	nM	IC50	J Med Chem (2010) 53: 5129-5143 [PMID:20575555]
ChEMBL	Concentration required to inhibit 50% of Acetylcholinesterase obtained from human erythrocytes was determined in vitro	B	8.1	pIC50	8	nM	IC50	J Med Chem (1995) 38: 1084-1089 [PMID:7707311]
ChEMBL	Enzyme Assay: The potential of the compounds of the present invention to inhibit acetylcholinesterase and butyrylcholinesterase enzymes were tested according to the method of Ellman et.al. (i.e., Ellman, G. L., Courtney, K. D., Andres, V., and Featherstone, R. M., Biochem. Pharmacol., 1961, 7, 88-95).Human recombinant acetylcholinesterase and human recombinant butyrylcholinesterase were employed. Each reaction mixture contained the enzyme (either ACHE or BCHE) solution, DTNB and sample in Tris HCl buffer solution. The reactions were initiated by the addition of the substrate (either acetylthiocholine iodide or butyrylthiocholine iodide, respectively for ACHE and BCHE enzymes). The enzyme catalyzed formation of the yellow color was measured at 412 nm in terms of the calculation of enzyme activity concomitant to the presence of an inhibitor activity.	B	8.1	pIC50	8	nM	IC50	US-9586925-B2. 1-(dimethylamino)ethyl-substituted 6H-benzo[C]chromen-6-ones against senile dementia (2017)
ChEMBL	Evaluated for the in vitro inhibition of the Acetylcholinesterase (AChE) from human erythrocytes	B	8.1	pIC50	8	nM	IC50	J Med Chem (1994) 37: 2721-2734 [PMID:8064800]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase (AChE) in human erythrocytes	B	8.1	pIC50	8	nM	IC50	Bioorg Med Chem Lett (1998) 8: 575-580 [PMID:9871563]
ChEMBL	Inhibition of AChE	B	8.1	pIC50	8	nM	IC50	Eur J Med Chem (2010) 45: 1167-1172 [PMID:20053484]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine iodide substrate incubated for 15 mins by spectrophotometry based Ellman's method	B	8.1	pIC50	8	nM	IC50	Bioorg Med Chem (2014) 22: 5141-5154 [PMID:25189690]
ChEMBL	Inhibition against Acetylcholinesterase (AChE)	B	8.1	pIC50	8	nM	IC50	J Med Chem (1996) 39: 380-387 [PMID:8558505]
ChEMBL	Inhibition of recombinant AChE in human erythrocytes using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman's method	B	8.13	pIC50	7.39	nM	IC50	Eur J Med Chem (2022) 229: 114045-114045 [PMID:34922191]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate peincubated for 5 mins followed by substrate addition by DTNB-reagent based Ellman's method	B	8.15	pIC50	7	nM	IC50	Eur J Med Chem (2021) 210: 113059-113059 [PMID:33310288]
ChEMBL	Inhibition of AchE (unknown origin)	B	8.15	pIC50	7	nM	IC50	ACS Med Chem Lett (2016) 7: 341-344 [PMID:27096037]
ChEMBL	Inhibition of human recombinant AChE using acetylthiocholine as substrate by UV-visible spectrophotometric Ellman's method	B	8.16	pIC50	6.87	nM	IC50	Bioorg Med Chem Lett (2018) 28: 2910-2913 [PMID:30017317]
ChEMBL	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate by Ellman's method	B	8.21	pIC50	6.2	nM	IC50	Medchemcomm (2019) 10: 1018-1026 [PMID:31304000]
ChEMBL	Inhibition of human erythrocytes AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured at 1 min time interval for 10 mins by Ellman's method	B	8.22	pIC50	6	nM	IC50	Eur J Med Chem (2019) 162: 234-248 [PMID:30447434]
ChEMBL	Inhibition of recombinant human AChE using ATCI as substrate incubated for 5 mins followed by substrate addition and measured after 5 mins by spectrophotometric based Ellman's method	B	8.22	pIC50	6	nM	IC50	Eur J Med Chem (2020) 207: 112743-112743 [PMID:32882609]
ChEMBL	Inhibition of AChE (unknown origin) using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by Ellman's method	B	8.22	pIC50	6	nM	IC50	RSC Med Chem (2020) 11: 307-316 [PMID:33479638]
ChEMBL	Inhibition of human recombinant AChE preincubated for 5 mins followed by acetylthiocholine iodide substrate addition measured after 5 mins by Ellman's method	B	8.22	pIC50	6	nM	IC50	Eur J Med Chem (2016) 124: 63-81 [PMID:27560283]
ChEMBL	Inhibition of human recombinant AChE after 5 mins by Ellman's method	B	8.22	pIC50	6	nM	IC50	Eur J Med Chem (2015) 92: 738-749 [PMID:25621991]
ChEMBL	Inhibition of human erythrocyte AChE pre-incubated for 5 mins before acetylthiocholine iodide substrate by Ellman' method	B	8.22	pIC50	6	nM	IC50	J Med Chem (2015) 58: 3172-3187 [PMID:25793650]
ChEMBL	Inhibition of human recombinant AChE pre-incubated for 5 mins before addition of acetylthiocholine iodide substrate by Ellman's assay	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem (2015) 23: 1629-1637 [PMID:25707322]
ChEMBL	Inhibition of AChE-induced amyloid beta aggregation	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2687-2691 [PMID:21216144]
ChEMBL	Inhibition of AChE (unknown origin)	B	8.24	pIC50	5.7	nM	IC50	Bioorg Med Chem (2013) 21: 676-683 [PMID:23273608]
ChEMBL	Inhibition of human AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured over 5 mins by UV-Vis spectrophotometric analysis	B	8.24	pIC50	5.7	nM	IC50	Eur J Med Chem (2017) 139: 773-791 [PMID:28863358]
ChEMBL	Inhibition of AChE (unknown origin)	B	8.24	pIC50	5.7	nM	IC50	Eur J Med Chem (2018) 147: 48-65 [PMID:29421570]
ChEMBL	Inhibition of recombinant human AChE using acetylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method	B	8.24	pIC50	5.7	nM	IC50	Eur J Med Chem (2018) 145: 431-444 [PMID:29335209]
ChEMBL	Inhibition of human acetylcholinesterase	B	8.24	pIC50	5.7	nM	IC50	Bioorg Med Chem (2019) 27: 895-930 [PMID:30744931]
ChEMBL	Inhibition of recombinant human AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured for 5 mins by Ellman's method	B	8.24	pIC50	5.7	nM	IC50	Bioorg Med Chem (2019) 27: 931-943 [PMID:30765302]
ChEMBL	Inhibition of recombinant human AChE using acetylthiocholine iodide as substrate preincubated with enzyme for 5 mins prior to substrate addition measured after 5 mins by Ellman's method	B	8.25	pIC50	5.59	nM	IC50	Eur J Med Chem (2020) 185: 111785-111785 [PMID:31669851]
GtoPdb	-	-	8.3	pIC50	-	-	-	J Med Chem (2006) 49: 2174-85 [PMID:16570913]; Eur J Pharmacol (1994) 270: 189-93 [PMID:8039548]; J Med Chem (1992) 35: 584-9 [PMID:1738151]
ChEMBL	Inhibition of human recombinant AChE expressed in HEK293 cells using acetylthiocholine iodide as substrate incubated for 10 mins by Ellman's method	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem (2021) 30: 115940-115940 [PMID:33340937]
ChEMBL	Inhibition of AChE (unknown origin) incubated for 30 mins by Ellman's method	B	8.4	pIC50	4	nM	IC50	Eur J Med Chem (2022) 233: 114242-114242 [PMID:35276424]
ChEMBL	Inhibition of human erythrocyte AChE using acetylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured every min for 10 mins by Ellman's method	B	8.41	pIC50	3.9	nM	IC50	RSC Med Chem (2024) 15: 963-980 [PMID:38516603]
ChEMBL	Inhibition of acetylcholinesterase (AChE) of human red blood cell (type XIII) by modified radiometric AChE assay	B	8.63	pIC50	2.33	nM	IC50	Bioorg Med Chem Lett (1992) 2: 861-864
ChEMBL	Inhibition of acetylcholinesterase (unknown origin)	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem (2013) 21: 146-152 [PMID:23199476]
ChEMBL	In vitro reversal of vecuronium-induced neuromuscular block in guinea pig hemi-diaphragm.	F	7.37	pEC50	43	nM	EC50	Bioorg Med Chem Lett (2002) 12: 2565-2568 [PMID:12182861]
Acetylcholinesterase in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4768] [UniProtKB: P23795]
ChEMBL	Inhibition of fetal Bovine serum AChE	B	8.54	pKi	2.9	nM	Ki	J Med Chem (2003) 46: 1-4 [PMID:12502352]
ChEMBL	Dissociation constant for inhibition of fetal bovine serum Acetylcholinesterase	B	8.54	pKi	2.9	nM	Ki	J Med Chem (2005) 48: 1919-1929 [PMID:15771436]
ChEMBL	Inhibition of bovine erythrocyte AChE by Ellman's assay	B	7.72	pIC50	19	nM	IC50	J Med Chem (2008) 51: 3588-3598 [PMID:18517184]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase (AChE) in bovine erythrocytes	B	8.1	pIC50	8	nM	IC50	Bioorg Med Chem Lett (1998) 8: 575-580 [PMID:9871563]
ChEMBL	Compound was evaluated for the inhibition of acetylcholinesterase (AChE) in bovine brain	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (1998) 8: 575-580 [PMID:9871563]
ChEMBL	Inhibition of bovine acetylcholinesterase using acetylcholine iodide as substrate incubated for 20 mins prior to substrate addition measured after 3 mins by Ellman's method	B	8.38	pIC50	4.2	nM	IC50	Bioorg Med Chem (2013) 21: 146-152 [PMID:23199476]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3198] [GtoPdb: 2465] [UniProtKB: P21836]
ChEMBL	Inhibition of AchE in albino LACA mouse brain using acetylthiocholine iodide as substrate incubated for 10 mins measured for 2 mins by Ellman's method	B	4.84	pIC50	14550	nM	IC50	Bioorg Med Chem (2018) 26: 215-224 [PMID:29195794]
ChEMBL	Inhibition of mouse AChE using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman's method	B	5.21	pIC50	6100	nM	IC50	J Med Chem (2021) 64: 7483-7506 [PMID:34024109]
ChEMBL	Inhibition of acetylcholinesterase in Albino LACA mouse brain homogenates using acetylthiocholine iodide as substrate measured for 2 mins by Ellman's method	B	5.74	pIC50	1820	nM	IC50	Bioorg Med Chem (2016) 24: 4587-4599 [PMID:27519464]
ChEMBL	In Vitro Pharmacodynamic Assay: Modified Ellman method (Alvin V. et al. JWS-USC-75-IX Improves Information Processing and Cognitive Function in Animal Models[J]. Journal of Pharmacology and experimental therapeutics, 2010, 336(3): 751) was employed in the test, for testing the inhibition effect of a series of compounds of formula (I) on acetylcholinesterase, so that active AchEIs were screened out, and their activity were evaluated via the IC50 value of inhibition of the series of compounds of formula (I) on the enzyme.	B	7.03	pIC50	94	nM	IC50	US-9346818-B2. Benzodioxole derivative and preparation method and use thereof (2016)
ChEMBL	Inhibition of mouse brain AChE using acetylthiocholine iodide as substrate measured after 30 mins by Ellman's method	B	7.08	pIC50	84	nM	IC50	Eur J Med Chem (2019) 168: 207-220 [PMID:30822710]
ChEMBL	Inhibition of mouse brain homogenate AChE using acetylthiocholine iodide as substrate measured after 15 mins in presence of BChE inhibitor methopropazine hydrochloride by DTNB reagent based colorimetric method	B	7.92	pIC50	11.9	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1593-1596 [PMID:31078410]
ChEMBL	Inhibition of AChE in mouse cortical homogenate using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman's method	B	8.21	pIC50	6.22	nM	IC50	Eur J Med Chem (2022) 229: 114045-114045 [PMID:34922191]
ChEMBL	Inhibition of AChE in mouse brain homogenate using acetylthiocholine as substrate preincubated for 300 secs followed by substrate addition by Ellman's method	B	8.24	pIC50	5.7	nM	IC50	J Med Chem (2018) 61: 119-139 [PMID:29227101]
ChEMBL	Inhibition of AChE in mouse cerebral cortex using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 180 secs by Ellman's spectrophotometric method	B	8.27	pIC50	5.4	nM	IC50	Eur J Med Chem (2022) 235: 114305-114305 [PMID:35339839]
acetylcholinesterase (Yt blood group)/Acetylcholinesterase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3199] [GtoPdb: 2465] [UniProtKB: P37136]
ChEMBL	Inhibition of AChE in rat brain using acetylthiocholine iodide as substrate by Ellman's UV-vis spectrophotometric method	B	6.92	pIC50	120	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127404-127404 [PMID:32717612]
ChEMBL	Inhibition of rat cortex AChE using acetylthiocholine iodide as substrate by Ellman's method	B	7.37	pIC50	42.82	nM	IC50	Eur J Med Chem (2021) 226: 113827-113827 [PMID:34530383]
ChEMBL	Inhibition of rat brain acetylcholine esterase in presence of acetylcholine substrate by chemiluminescent assay	B	7.4	pIC50	40	nM	IC50	Eur J Med Chem (2008) 43: 657-661 [PMID:17624631]
ChEMBL	Inhibition of rat cortex homogenate AChE using acetylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of 5% rat cortex homogenate AChE using acetylthiocholine chloride as substrate after 15 mins by Ellman's method	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem (2017) 25: 3006-3017 [PMID:28487125]
ChEMBL	Inhibition of acetylcholinesterase in Wistar rat brain homogenate by Ellman's method	B	7.45	pIC50	35.48	nM	IC50	Bioorg Med Chem (2008) 16: 9911-9924 [PMID:18976927]
ChEMBL	Inhibition of AChE in Sprague-Dawley rat cortices using acetylthiocholine iodide as substrate by Ellman's method	B	7.49	pIC50	32	nM	IC50	Bioorg Med Chem (2013) 21: 676-683 [PMID:23273608]
ChEMBL	Inhibition of AchE in rat cortex by Ellman's method	B	7.57	pIC50	26.8	nM	IC50	Bioorg Med Chem (2008) 16: 7646-7653 [PMID:18662884]
ChEMBL	Inhibition of AChE activity in rat brain homogenate using acetylthiocholine as substrate by Ellman's method	B	7.67	pIC50	21.5	nM	IC50	Bioorg Med Chem (2012) 20: 521-530 [PMID:22172310]
ChEMBL	Inhibition of AchE in Rattus norvegicus (rat) cortex homogenates by Ellman's method	B	7.67	pIC50	21.5	nM	IC50	Med Chem Res (2013) 22: 1648-1659
ChEMBL	Inhibitory activity against Acetylcholinesterase (AChE) in rat brain hippocampal crude homogenate	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (1999) 9: 3279-3284 [PMID:10612585]
ChEMBL	Inhibition of AChE in rat brain cortex homogenates using acetylthiocholine iodide as substrate in presence of BuChE inhibitor tetraisopropyl pyrophosphoramide measured after 15 mins by Ellman's method	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2017) 126: 762-775 [PMID:27951485]
ChEMBL	In vitro inhibitory concentration against rat cortex acetylcholinesterase	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem Lett (2005) 15: 3834-3837 [PMID:15993600]
ChEMBL	Inhibition of acetylcholinesterase in Rattus norvegicus (rat) cortex by Ellman method	B	7.8	pIC50	16	nM	IC50	Med Chem Res (2013) 22: 2328-2345
ChEMBL	Inhibition of acetylcholinesterase in Rattus norvegicus (rat) cortex by Ellman method	B	7.8	pIC50	15.85	nM	IC50	Med Chem Res (2013) 22: 2328-2345
ChEMBL	Inhibition of rat cortex homogenate AchE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2014) 76: 314-331 [PMID:24589487]
ChEMBL	Inhibition of rat cortex homogenate acetylcholinesterase by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2021) 216: 113310-113310 [PMID:33667847]
ChEMBL	Inhibition of rat cortex AChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2020) 187: 111958-111958 [PMID:31865014]
ChEMBL	Inhibition of AChE from rat cortex homogenate using acetylthiocholine iodide as substrate after 15 mins by Ellman method	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem (2015) 23: 911-923 [PMID:25678013]
ChEMBL	Inhibition of AChE in rat cortex using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2015) 94: 348-366 [PMID:25778991]
ChEMBL	Inhibition of rat cortex homogenate AChE using acetylthiocholine iodide as substrate after 15 mins in presence of BuChE inhibitor iso-OMPA by Ellman's method	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2017) 135: 307-323 [PMID:28458136]
ChEMBL	Inhibition of rat cortex AChE using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem (2018) 26: 6115-6127 [PMID:30470598]
ChEMBL	Inhibition of rat brain AChE using acetylthiocholine iodide as substrate by spectroscopic method	B	7.9	pIC50	12.7	nM	IC50	Bioorg Med Chem (2017) 25: 6273-6285 [PMID:29089261]
ChEMBL	Inhibition of AChE in rat cortex after 15 mins by modified Ellman's method	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem (2009) 17: 6692-6698 [PMID:19692250]
ChEMBL	Inhibition of AChE in rat cortex after 15 mins by modified Ellman method	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2009) 44: 7-17 [PMID:18436348]
ChEMBL	Inhibition of AChE in rat cortex homogenates using acetylthiocholine iodide substrate incubated for 15 mins by UV spectroscopy based Ellman's method	B	7.96	pIC50	11	nM	IC50	Bioorg Med Chem (2014) 22: 4717-4725 [PMID:25082512]
ChEMBL	Inhibition of rat cortex homogenate acetylcholinesterase by spectrophotometric method	B	8	pIC50	10	nM	IC50	Bioorg Med Chem (2020) 28: 115820-115820 [PMID:33120080]
ChEMBL	Inhibition of AChE in rat cortex preincubated for 20 mins by Ellman method	B	8.1	pIC50	7.86	nM	IC50	Bioorg Med Chem (2009) 17: 1600-1613 [PMID:19162488]
ChEMBL	Inhibitory concentration in vitro and ex vivo for anti-AChE activity in rat brain	B	8.24	pIC50	5.7	nM	IC50	Bioorg Med Chem Lett (1992) 2: 871-876
ChEMBL	Inhibition of rat cortex AChE	B	8.24	pIC50	5.7	nM	IC50	J Med Chem (2008) 51: 3588-3598 [PMID:18517184]
ChEMBL	Inhibition of AChE in rat cortex homogenates using acetylthiocholine iodide as substrate after 15 mins by Ellman's method	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem (2011) 19: 7158-7167 [PMID:22019465]
ChEMBL	Inhibition of rat cortex homogenate acetylcholinesterase using acetylthiocholine iodide as substrate incubated for 20 mins by Ellman's method	B	8.7	pIC50	2	nM	IC50	J Med Chem (2021) 64: 1844-1855 [PMID:33570950]
ChEMBL	In vitro inhibitory effect on rat Acetylcholinesterase	B	8.77	pIC50	1.7	nM	IC50	J Med Chem (1995) 38: 2969-2973 [PMID:7636858]
Monoamine oxidase B/Amine oxidase [flavin-containing] B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2039] [GtoPdb: 2490] [UniProtKB: P27338]
ChEMBL	Inhibition of human recombinant MAO-B incubated for 30 mins by fluorescence based assay	B	4.64	pIC50	23000	nM	IC50	Eur J Med Chem (2022) 233: 114242-114242 [PMID:35276424]
ChEMBL	Inhibition of human recombinant MAOB using p-tyramine as substrate preincubated for 30 mins followed by substrate addition measured for 1 hr by horse-radish peroxidase/amplex red-based fluorometric method	B	4.82	pIC50	15200	nM	IC50	Eur J Med Chem (2016) 121: 864-879 [PMID:26471320]
ChEMBL	Inhibition of human recombinant MAO-B using tyramine as substrate preincubated for 30 mins followed by substrate addition measured for 1 hr by fluorometric assay	B	4.82	pIC50	15000	nM	IC50	Eur J Med Chem (2014) 75: 82-95 [PMID:24530494]
Monoamine oxidase B/Amine oxidase [flavin-containing] B in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2993] [GtoPdb: 2490] [UniProtKB: P19643]
ChEMBL	Inhibition of rat liver mitochondrial MAO-B using [14C]-phenylethylamine after 30 mins by scintillation counting	B	4.82	pIC50	15000	nM	IC50	J Med Chem (2011) 54: 8251-8270 [PMID:22023459]
ChEMBL	Inhibition of mitochondrial MAO-B in rat liver homogenates using [14C]-phenylethylamine as substrate preincubated for 30 mins by liquid scintillation counting	B	4.82	pIC50	15000	nM	IC50	Eur J Med Chem (2012) 52: 251-262 [PMID:22503231]
ChEMBL	Inhibition of MAO-B in rat liver homogenate using [14C]-phenylethylamine as substrate preincubated for 30 mins followed by substrate addition measured after 4 mins by liquid scintillation counting analysis	B	4.82	pIC50	15000	nM	IC50	Eur J Med Chem (2014) 80: 543-561 [PMID:24813882]
Amyloid-beta precursor protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2487] [UniProtKB: P05067]
ChEMBL	Inhibition of self mediated Amyloid beta (1 to 42) (unknown origin) aggregation measured every 5 mins by ThT fluorescence based assay	B	4.06	pIC50	87410	nM	IC50	Eur J Med Chem (2023) 262: 115880-115880 [PMID:37871406]
ABCB1/ATP-dependent translocase ABCB1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4302] [GtoPdb: 768] [UniProtKB: P08183]
ChEMBL	Inhibition of human recombinant MDR1 in cell membrane fraction preincubated for 5 mins measured after 40 mins by Pgp-Glo luciferase assay	B	4.46	pIC50	34850	nM	IC50	Bioorg Med Chem Lett (2015) 25: 297-301 [PMID:25499431]
beta-secretase 1/Beta-secretase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4822] [GtoPdb: 2330] [UniProtKB: P56817]
ChEMBL	Inhibition of BACE1	B	5.49	pIC50	3200	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4707-4712 [PMID:22704921]
ChEMBL	Inhibition of human BACE-1 using (7-Methoxycoumarin-4-acetyl-[Asn670, Leu671]-Amyloid beta/A4 precursor protein 770 fragments 667-676- (2, 4-dinitrophenyl) Lys-Arg-Arg amide trifluoroacetate salt as substrate incubated for 2 hrs by FRET-based analysis	B	5.9	pIC50	1247	nM	IC50	Eur J Med Chem (2024) 271: 116409-116409 [PMID:38663285]
ChEMBL	Inhibition of human BACE-1 by FRET assay	B	6.44	pIC50	362	nM	IC50	Bioorg Med Chem (2020) 28: 115721-115721 [PMID:33007563]
ChEMBL	Inhibition of human BACE-1 using MOCA-SEV-NL-DAEFR-DNP-RR as substrate preincubated with substrate followed by enzyme addition by FRET assay	B	6.44	pIC50	362	nM	IC50	Eur J Med Chem (2019) 183: 111707-111707 [PMID:31561043]
ChEMBL	Inhibition of human BACE1 using as 7-Methoxycoumarin-4-acetyl-[Asn670, Leu671]-Amyloid beta/A4 precursor protein 770 fragment 667-676-(2,4-dinitrophenyl) Lys-Arg-Arg amide trifluoroacetate salt as substrate incubated for 2 hrs by FRET based florescence assay	B	6.62	pIC50	240	nM	IC50	Eur J Med Chem (2019) 167: 510-524 [PMID:30784883]
ChEMBL	Inhibition of human BACE-1 measured after 2 hrs by FRET assay	B	6.64	pIC50	227	nM	IC50	Eur J Med Chem (2024) 271: 116450-116450 [PMID:38701714]
ChEMBL	Inhibition of BACE1 (unknown origin)	B	6.77	pIC50	169	nM	IC50	Eur J Med Chem (2017) 138: 738-747 [PMID:28728106]
ChEMBL	Inhibition of human recombinant BACE1	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2021) 221: 113492-113492 [PMID:33984802]
Butyrylcholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5077] [UniProtKB: Q9N1N9]
ChEMBL	Inhibition of Equine Butyrylcholinesterase	B	6.19	pKi	640	nM	Ki	J Med Chem (2003) 46: 1-4 [PMID:12502352]
ChEMBL	Dissociation constant for inhibition of equine Butyrylcholinesterase (BuChE)	B	6.19	pKi	640	nM	Ki	J Med Chem (2005) 48: 1919-1929 [PMID:15771436]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate after 15 mins by Ellman's method	B	5.13	pIC50	7400	nM	IC50	Eur J Med Chem (2012) 52: 251-262 [PMID:22503231]
ChEMBL	Inhibition Assay: The inhibitory activity of the enzyme acetylcholinesterase (AChE) was evaluated by the Ellman method (Biochem. Pharmacol. 1961, 7, 88) using an electric eel as an AChE neuronal model (Electrophorus electricus) and acetylthiocholine iodide (0.35 mM) as substrate. The reaction took place in a final volume of 3 mL of a 0.1 M phosphate buffer solution, pH 8.0, containing 0.035 units of AChE and used a 0.35 mM solution of 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) to produce the 5-thio-2-nitrobenzoic acid anion. Inhibition curves were performed in triplicate by incubating at least nine inhibitor concentrations for 10 min. A triplicate sample without inhibitor was always present so as to be aware of 100% of the AChE activity. After this time, the substrate was added to 0.35 mM acetylthiocholine iodide, from a 10 mM stock solution. Loss of color was observed at 412 nm in a spectrophotometric reader having 96 well plates.	B	5.13	pIC50	7400	nM	IC50	US-8999994-B2. Derivatives of propargylamine having neuroprotective capacity for the treatment of Alzheimer's and Parkinson's diseases (2015)
ChEMBL	Inhibition of (BChE) Butyrylcholinesterase of horse serum	B	5.38	pIC50	4166.7	nM	IC50	Bioorg Med Chem Lett (1992) 2: 861-864
ChEMBL	Inhibition of equine BChE preincubated for 15 mins by Ellman's method	B	5.47	pIC50	3365.4	nM	IC50	Eur J Med Chem (2014) 87: 429-439 [PMID:25282266]
ChEMBL	Compound was evaluated for the in vitro inhibition of the Butyrylcholinesterase from horse serum	B	5.54	pIC50	2900	nM	IC50	J Med Chem (1994) 37: 2721-2734 [PMID:8064800]
Butyrylcholinesterase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3403] [UniProtKB: Q9JKC1]
ChEMBL	Inhibition of rat serum butyrylcholinesterase using butyrylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	4.67	pIC50	21400	nM	IC50	Bioorg Med Chem (2019) 27: 895-930 [PMID:30744931]
ChEMBL	Inhibition of rat serum BuChE measured after 15 mins by Ellman's method	B	4.67	pIC50	21400	nM	IC50	Bioorg Med Chem (2017) 25: 714-726 [PMID:27923535]
ChEMBL	Inhibition of rat serum BuChE using butyrylthiocholine iodide as substrate after 15 mins by Ellman's method	B	4.68	pIC50	20700	nM	IC50	Eur J Med Chem (2014) 76: 314-331 [PMID:24589487]
ChEMBL	Inhibition of rat serum BuChE by Ellman's method	B	4.68	pIC50	20700	nM	IC50	Eur J Med Chem (2021) 216: 113310-113310 [PMID:33667847]
ChEMBL	Inhibition of rat serum BChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	4.68	pIC50	20700	nM	IC50	Eur J Med Chem (2020) 187: 111958-111958 [PMID:31865014]
ChEMBL	Inhibition of rat serum BChE using butyrylthiocholine iodide as substrate by Ellman's method	B	4.89	pIC50	12773	nM	IC50	Eur J Med Chem (2021) 226: 113827-113827 [PMID:34530383]
ChEMBL	Inhibition of rat serum BuChE using S-butyrylthiocholine iodide as substrate incubated for 8 mins by Ellman's method	B	4.97	pIC50	10833.33	nM	IC50	J Med Chem (2021) 64: 1844-1855 [PMID:33570950]
ChEMBL	In vitro inhibitory concentration against butyrylcholinesterase was determined using rat serum homogenate	B	5.12	pIC50	7600	nM	IC50	Bioorg Med Chem Lett (2005) 15: 3834-3837 [PMID:15993600]
ChEMBL	Inhibitory concentration was determined in in vitro for Butyrylcholinesterase in rat plasma	B	5.15	pIC50	7138	nM	IC50	Bioorg Med Chem Lett (1992) 2: 871-876
ChEMBL	Inhibition of rat serum BChE	B	5.15	pIC50	7138	nM	IC50	J Med Chem (2008) 51: 3588-3598 [PMID:18517184]
ChEMBL	Inhibition of rat plasma butyrylcholine esterase in presence of butyrylcholine substrate by chemiluminescent assay	B	5.28	pIC50	5300	nM	IC50	Eur J Med Chem (2008) 43: 657-661 [PMID:17624631]
ChEMBL	Inhibition of rat cortex homogenate BuChE by spectrophotometric method	B	5.3	pIC50	5010	nM	IC50	Bioorg Med Chem (2020) 28: 115820-115820 [PMID:33120080]
ChEMBL	Inhibition of BChE in rat serum after 15 mins by modified Ellman's method	B	5.33	pIC50	4670	nM	IC50	Bioorg Med Chem (2009) 17: 6692-6698 [PMID:19692250]
ChEMBL	Inhibition of rat serum BChE using butyrylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	5.35	pIC50	4420	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of BuchE in rat serum by Ellman's method	B	5.49	pIC50	3260	nM	IC50	Bioorg Med Chem (2008) 16: 7646-7653 [PMID:18662884]
ChEMBL	Inhibition of BChE in rat serum after 15 mins by modified Ellman method	B	5.56	pIC50	2770	nM	IC50	Eur J Med Chem (2009) 44: 7-17 [PMID:18436348]
ChEMBL	Inhibition of BChE in rat serum using butyrylthiocholine iodide substrate incubated for 15 mins by UV spectroscopy based Ellman's method	B	5.89	pIC50	1280	nM	IC50	Bioorg Med Chem (2014) 22: 4717-4725 [PMID:25082512]
phosphodiesterase 5A/cGMP-specific 3`,5`-cyclic phosphodiesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1827] [GtoPdb: 1304] [UniProtKB: O76074]
ChEMBL	Inhibition of PDE5A (unknown origin)	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127097-127097 [PMID:32171616]
ChEMBL	Inhibition of PDE5A1 (unknown origin) using [3H]-cGMP substrate incubated for 15 mins by liquid scintillation counting method	B	7.96	pIC50	11	nM	IC50	Eur J Med Chem (2024) 271: 116386-116386 [PMID:38614063]
choline O-acetyltransferase/Choline O-acetyltransferase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3945] [GtoPdb: 2480] [UniProtKB: P32738]
ChEMBL	In vitro inhibitory activity against acetylcholinesterase	B	8.28	pIC50	5.3	nM	IC50	J Med Chem (1992) 35: 584-589 [PMID:1738151]
butyrylcholinesterase/Cholinesterase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1914] [GtoPdb: 2471] [UniProtKB: P06276]
ChEMBL	Non competitive inhibition of human serum BChE using butyrylthiocholine iodide as substrate by Lineweaver-Burk plot analysis	B	4.9	pKi	12540	nM	Ki	Bioorg Med Chem (2013) 21: 5451-5460 [PMID:23810675]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins prior to substrate addition by Ellman's method	B	4.82	pIC50	15240	nM	IC50	Bioorg Med Chem (2013) 21: 5451-5460 [PMID:23810675]
ChEMBL	Inhibition of human serum BChE using butyrylthiolcholine iodide as substrate incubated for 20 mins prior to substrate addition by Ellman's method	B	4.82	pIC50	15240	nM	IC50	Bioorg Med Chem Lett (2013) 23: 2984-2989 [PMID:23562059]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as substrate after 10 mins measured for every mins by Ellman's method	B	4.82	pIC50	15240	nM	IC50	Eur J Med Chem (2019) 163: 116-135 [PMID:30503937]
ChEMBL	Inhibition of human plasma BuChE using butyrylthiocholine as substrate measured after 5 mins by Ellman's method	B	4.84	pIC50	14400	nM	IC50	Eur J Med Chem (2020) 191: 112140-112140 [PMID:32088494]
ChEMBL	Inhibition of human BuChE using S-butyrylthiocholine iodide as substrate treated 5 mins before substrate addition measured up to 4 mins by Ellman's method	B	4.87	pIC50	13600	nM	IC50	Bioorg Med Chem Lett (2013) 23: 4336-4341 [PMID:23791077]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	4.93	pIC50	11860	nM	IC50	Eur J Med Chem (2021) 223: 113735-113735 [PMID:34371367]
ChEMBL	Inhibition of human recombinant BuChe using butyrylthiocholine iodide as substrate incubated for 10 mins by DTNB reagent based Ellman's method	B	4.97	pIC50	10660	nM	IC50	J Med Chem (2022) 65: 12292-12318 [PMID:36084304]
ChEMBL	Inhibition of BuChE (unknown origin) by Ellman's method	B	4.97	pIC50	10600	nM	IC50	RSC Med Chem (2024) 15: 207-222 [PMID:38283217]
ChEMBL	Inhibition of human plasma BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured at 6 mins by Ellman's method	B	4.99	pIC50	10240	nM	IC50	Bioorg Med Chem (2019) 27: 3650-3662 [PMID:31288978]
ChEMBL	Inhibition of human BChE by Ellman's method	B	5	pIC50	10050	nM	IC50	Bioorg Med Chem (2007) 15: 6596-6607 [PMID:17681794]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured over 5 mins by UV-Vis spectrophotometric analysis	B	5.04	pIC50	9140	nM	IC50	Eur J Med Chem (2017) 139: 773-791 [PMID:28863358]
ChEMBL	Inhibition of recombinant human BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured for 5 mins by Ellman's method	B	5.04	pIC50	9140	nM	IC50	Bioorg Med Chem (2019) 27: 931-943 [PMID:30765302]
ChEMBL	Inhibition of recombinant human serum BuChE using butyrylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method	B	5.04	pIC50	9140	nM	IC50	Eur J Med Chem (2018) 145: 431-444 [PMID:29335209]
ChEMBL	Inhibition of human serum BuChE using S-butyrylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by Ellman's method	B	5.06	pIC50	8700	nM	IC50	Eur J Med Chem (2017) 139: 48-59 [PMID:28797883]
ChEMBL	Inhibition of human serum butyrylcholinesterase preincubated for 5 mins followed by addition of S-butyrylthiocholine iodide as substrate measured after 60 to 120 mins by Ellmans method	B	5.08	pIC50	8280	nM	IC50	Medchemcomm (2016) 7: 990-998
ChEMBL	Inhibitory concentration against Butyrylcholinesterase (BuChE) from human serum	B	5.09	pIC50	8200	nM	IC50	J Med Chem (1999) 42: 730-741 [PMID:10052979]
ChEMBL	Inhibition of recombinant human BuChE expressed in HEK293 cells preincubated for 15 mins	B	5.09	pIC50	8200	nM	IC50	J Med Chem (2014) 57: 10455-10463 [PMID:25418133]
ChEMBL	Inhibition of butyrylcholinesterase from human serum	B	5.09	pIC50	8200	nM	IC50	J Med Chem (2001) 44: 2707-2718 [PMID:11495583]
ChEMBL	Inhibition of human serum BChE by Ellman's method	B	5.12	pIC50	7600	nM	IC50	Eur J Med Chem (2020) 192: 112180-112180 [PMID:32131034]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated followed by substrate addition by Ellman's method	B	5.12	pIC50	7600	nM	IC50	Bioorg Med Chem (2016) 24: 1050-1062 [PMID:26827140]
ChEMBL	Inhibition of human recombinant BChE	B	5.12	pIC50	7585.78	nM	IC50	J Med Chem (2004) 47: 6490-6498 [PMID:15588084]
ChEMBL	Inhibition of human recombinant BuChE expressed in HEK293 cells using S-butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman's method	B	5.12	pIC50	7500	nM	IC50	Eur J Med Chem (2016) 121: 864-879 [PMID:26471320]
ChEMBL	Inhibition of human serum BChE by Ellman's assay	B	5.13	pIC50	7420	nM	IC50	J Med Chem (2008) 51: 7308-7312 [PMID:18954037]
ChEMBL	Inhibitory potency was evaluated against isolated Butyrylcholinesterase	B	5.13	pIC50	7420	nM	IC50	J Med Chem (2003) 46: 2279-2282 [PMID:12773032]
ChEMBL	Inhibition of human recombinant butyrylcholinesterase	B	5.13	pIC50	7420	nM	IC50	J Med Chem (2007) 50: 4882-4897 [PMID:17850125]
ChEMBL	Inhibition of human serum BChE by Ellman's method	B	5.13	pIC50	7420	nM	IC50	Eur J Med Chem (2009) 44: 1341-1348 [PMID:18396354]
ChEMBL	Inhibition of human BuChE preincubated for 20 mins before substrate addition by Ellman's method	B	5.13	pIC50	7420	nM	IC50	Bioorg Med Chem (2010) 18: 1749-1760 [PMID:20171894]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate by Ellman's method	B	5.13	pIC50	7400	nM	IC50	Eur J Med Chem (2011) 46: 1682-1693 [PMID:21397996]
ChEMBL	Inhibition of human BChE using butyrylthiocholineiodide as substrate incubated for 10 mins by Ellman's method	B	5.14	pIC50	7300	nM	IC50	Bioorg Med Chem (2021) 30: 115940-115940 [PMID:33340937]
ChEMBL	Enzyme Assay: The potential of the compounds of the present invention to inhibit acetylcholinesterase and butyrylcholinesterase enzymes were tested according to the method of Ellman et.al. (i.e., Ellman, G. L., Courtney, K. D., Andres, V., and Featherstone, R. M., Biochem. Pharmacol., 1961, 7, 88-95).Human recombinant acetylcholinesterase and human recombinant butyrylcholinesterase were employed. Each reaction mixture contained the enzyme (either ACHE or BCHE) solution, DTNB and sample in Tris HCl buffer solution. The reactions were initiated by the addition of the substrate (either acetylthiocholine iodide or butyrylthiocholine iodide, respectively for ACHE and BCHE enzymes). The enzyme catalyzed formation of the yellow color was measured at 412 nm in terms of the calculation of enzyme activity concomitant to the presence of an inhibitor activity.	B	5.15	pIC50	7100	nM	IC50	US-9586925-B2. 1-(dimethylamino)ethyl-substituted 6H-benzo[C]chromen-6-ones against senile dementia (2017)
ChEMBL	Inhibition of human recombinant BuChE using butyrylthiocholine iodide substrate incubated for 15 mins by spectrophotometry based Ellman's method	B	5.15	pIC50	7100	nM	IC50	Bioorg Med Chem (2014) 22: 5141-5154 [PMID:25189690]
ChEMBL	Inhibition of BChE	B	5.15	pIC50	7000	nM	IC50	Bioorg Med Chem Lett (2011) 21: 2687-2691 [PMID:21216144]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as a substrate preincubated for 20 mins followed by substrate addition by spectrophotometric analysis	B	5.16	pIC50	6980	nM	IC50	Eur J Med Chem (2021) 225: 113779-113779 [PMID:34418785]
ChEMBL	Inhibition of BuChE (unknown origin) after 5 mins by Ellman's method	B	5.16	pIC50	6920	nM	IC50	Eur J Med Chem (2014) 83: 355-365 [PMID:24980117]
ChEMBL	Inhibition of BuChE (unknown origin) using acetylthiocholine iodide as substrate incubated for 5 mins by Ellman's method	B	5.19	pIC50	6500	nM	IC50	Eur J Med Chem (2022) 227: 113964-113964 [PMID:34743062]
ChEMBL	Inhibition of recombinant human BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	5.19	pIC50	6400	nM	IC50	Eur J Med Chem (2021) 212: 113124-113124 [PMID:33395623]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	5.2	pIC50	6370	nM	IC50	Eur J Med Chem (2014) 78: 167-177 [PMID:24681981]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins before substrate addition after 15 mins by Ellman's method	B	5.2	pIC50	6370	nM	IC50	Eur J Med Chem (2014) 78: 43-53 [PMID:24675179]
ChEMBL	Inhibition of human serum BuChE using S-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by Ellman's method	B	5.25	pIC50	5600	nM	IC50	Eur J Med Chem (2015) 103: 302-311 [PMID:26363866]
ChEMBL	Inhibition of BuChE (unknown origin) preincubated for 30 mins followed by substrate addition acetylthiocholineiodide measured after 40 mins by Ellman's method	B	5.27	pIC50	5400	nM	IC50	Eur J Med Chem (2020) 200: 112415-112415 [PMID:32454229]
ChEMBL	Inhibition of human BuChE using butyrylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	5.29	pIC50	5120	nM	IC50	Eur J Med Chem (2019) 183: 111737-111737 [PMID:31581002]
ChEMBL	Inhibition of human BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB based Ellman's method	B	5.29	pIC50	5100	nM	IC50	Eur J Med Chem (2024) 270: 116353-116353 [PMID:38579622]
ChEMBL	Inhibition of human serum BuChE using butylthiocholine chloride as substrate pretreated for 15 mins followed by substrate addition measured for 2 mins by DTNB reagent based spectrophotometric method	B	5.3	pIC50	>5000	nM	IC50	Eur J Med Chem (2017) 130: 139-153 [PMID:28242549]
ChEMBL	Inhibition of human BChE using butyrylthiocholine as substrate preincubated for 20 mins followed by substrate addition and measured upto 5 mins by Ellman's method	B	5.32	pIC50	4800	nM	IC50	Eur J Med Chem (2019) 177: 414-424 [PMID:31158754]
ChEMBL	Inhibition of human serum BuChE by spectrophotometric Ellman's method	B	5.32	pIC50	4800	nM	IC50	J Med Chem (2015) 58: 5561-5578 [PMID:26107513]
ChEMBL	Inhibition of human recombinant BChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by DTNB reagent based Ellman's method	B	5.32	pIC50	4800	nM	IC50	Eur J Med Chem (2023) 250: 115169-115169 [PMID:36753881]
ChEMBL	Inhibition of human BChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured for 5 mins by Ellman's spectrophotometric method	B	5.32	pIC50	4800	nM	IC50	Bioorg Med Chem (2023) 84: 117256-117256 [PMID:37003157]
ChEMBL	Inhibition of human BuChE by Ellman's spectrophotometric method	B	5.32	pIC50	4800	nM	IC50	Eur J Med Chem (2022) 237: 114358-114358 [PMID:35462163]
ChEMBL	Inhibition of human serum recombinant BChE using butyrylthiocholine iodide as a substrate incubated for 20 mins by DTNB reagent based Ellman's method	B	5.32	pIC50	4800	nM	IC50	RSC Med Chem (2022) 13: 873-883 [PMID:35923722]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by DTNB reagent based Ellman's method	B	5.32	pIC50	4786.3	nM	IC50	Bioorg Med Chem (2019) 27: 1327-1340 [PMID:30795991]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of human serum BCHE preincubated for 6 mins followed by addition of S-butyrylcholine iodide as substrate by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Bioorg Med Chem (2016) 24: 4324-4338 [PMID:27460699]
ChEMBL	Inhibition of human BChE by Ellman's method	B	5.34	pIC50	4610	nM	IC50	Eur J Med Chem (2024) 274: 116511-116511 [PMID:38820854]
ChEMBL	Inhibition of BChE (unknown origin) by Ellman's method	B	5.34	pIC50	4530	nM	IC50	Bioorg Med Chem Lett (2022) 75: 128976-128976 [PMID:36067929]
ChEMBL	Inhibition of human serum BChE using S-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 180 secs by spectrophotometric analysis	B	5.36	pIC50	4380	nM	IC50	Eur J Med Chem (2017) 139: 68-83 [PMID:28800459]
ChEMBL	Inhibition of BuchE (unknown origin)	B	5.37	pIC50	4260	nM	IC50	Bioorg Med Chem Lett (2022) 61: 128602-128602 [PMID:35124202]
ChEMBL	Anticholinesterase activity against human plasma BChE	B	5.38	pIC50	4150	nM	IC50	J Med Chem (2006) 49: 2174-2185 [PMID:16570913]
ChEMBL	Inhibition of BChE (unknown origin)	B	5.38	pIC50	4150	nM	IC50	Eur J Med Chem (2018) 156: 598-617 [PMID:30031971]
ChEMBL	Ex vivo inhibition of human plasma Butyrylcholinesterase.	B	5.38	pIC50	4150	nM	IC50	J Med Chem (2002) 45: 3684-3691 [PMID:12166941]
ChEMBL	Inhibitory concentration against human plasma Butyrylcholinesterase	B	5.38	pIC50	4150	nM	IC50	J Med Chem (2005) 48: 986-994 [PMID:15715468]
ChEMBL	Inhibition of human BChE after 20 mins using butyrylthiocholine iodide as a substrate by Ellman's assay	B	5.41	pIC50	3900	nM	IC50	J Med Chem (2011) 54: 2627-2645 [PMID:21417225]
ChEMBL	Inhibition of human BuChE using S-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 60 to 300 sec by Ellman's method	B	5.44	pIC50	3600	nM	IC50	Medchemcomm (2015) 6: 1930-1941
ChEMBL	Inhibition of BuChE in human serum using butyrylthiocholine chloride as substrate measured after 15 mins by Ellman's method	B	5.49	pIC50	3200	nM	IC50	Eur J Med Chem (2017) 130: 379-392 [PMID:28279845]
ChEMBL	Inhibition of human serum BChE using acetylthiocholine iodide as substrate measured after 15 mins by Ellman's method	B	5.49	pIC50	3200	nM	IC50	Eur J Med Chem (2020) 187: 111958-111958 [PMID:31865014]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition measured after 180 secs by Ellman's method	B	5.5	pIC50	3170	nM	IC50	Eur J Med Chem (2018) 157: 161-176 [PMID:30096650]
ChEMBL	Inhibition of human serum BuChE using s-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 3 mins by Ellman's method	B	5.5	pIC50	3170	nM	IC50	Eur J Med Chem (2016) 123: 282-297 [PMID:27484514]
ChEMBL	Inhibition of BChE (unknown origin) incubated for 30 mins by Ellman's method	B	5.5	pIC50	3130	nM	IC50	Eur J Med Chem (2022) 233: 114242-114242 [PMID:35276424]
ChEMBL	Concentration required to inhibit 50% of Butyrylcholinesterase obtained from human serum was determined in vitro	B	5.54	pIC50	2900	nM	IC50	J Med Chem (1995) 38: 1084-1089 [PMID:7707311]
ChEMBL	Inhibition of human BChE using butyrylthiocholineiodide as substrate measured for every 30 sec for 5 mins by Ellman's method	B	5.54	pIC50	2900	nM	IC50	ACS Med Chem Lett (2020) 11: 869-876 [PMID:32435398]
ChEMBL	Inhibition of human serum BuChE using S-butyrylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by spectrophotometric-based Ellman's method	B	5.56	pIC50	2740	nM	IC50	Bioorg Med Chem (2016) 24: 1528-1539 [PMID:26917219]
ChEMBL	Inhibition of C-terminal 6His-tagged human recombinant BuChE (29 to 602 residues) using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by Ellman's method	B	5.59	pIC50	2580	nM	IC50	Eur J Med Chem (2022) 231: 114152-114152 [PMID:35101650]
ChEMBL	Inhibition of human BuChE measured after 15 to 20 mins by DTNB reagent based Ellman's method	B	5.59	pIC50	2580	nM	IC50	Eur J Med Chem (2023) 262: 115880-115880 [PMID:37871406]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2016) 121: 376-386 [PMID:27267007]
ChEMBL	Inhibition of human plasma BuChE using butyrylthiocholine as substrate by Ellman's method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2013) 67: 64-74 [PMID:23838422]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine as substrate pretreated for 5 mins followed by substrate addition and measured for 5 mins by Ellman's method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2018) 156: 534-553 [PMID:30025348]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine as substrate by Ellman method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2012) 57: 296-301 [PMID:23078965]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured for 5 mins by UV-Vis spectrophotometric method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2017) 130: 60-72 [PMID:28242552]
ChEMBL	Inhibition of human serum BuChE using butyrylthiocholine as substrate pretreated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2018) 158: 781-800 [PMID:30245401]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by Ellman's method	B	5.62	pIC50	2420	nM	IC50	Eur J Med Chem (2019) 183: 111707-111707 [PMID:31561043]
ChEMBL	Inhibition of human BChE by Ellman's method	B	5.62	pIC50	2420	nM	IC50	Bioorg Med Chem (2020) 28: 115721-115721 [PMID:33007563]
ChEMBL	Inhibition of human BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by DTNB reagent based Ellman's method	B	5.65	pIC50	2240	nM	IC50	Eur J Med Chem (2022) 242: 114630-114630 [PMID:35987018]
ChEMBL	Inhibition of butyrylcholinesterase (unknown origin)	B	5.7	pIC50	2000	nM	IC50	Bioorg Med Chem (2013) 21: 146-152 [PMID:23199476]
ChEMBL	Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by DTNB based Ellman's method	B	5.85	pIC50	1400	nM	IC50	Eur J Med Chem (2024) 271: 116450-116450 [PMID:38701714]
ChEMBL	Inhibition of human serum butyrylcholinesterase using BTCI as substrate pre-incubated for 10 mins followed by substrate addition and measured for 6 mins by Ellman's method	B	5.85	pIC50	1400	nM	IC50	Eur J Med Chem (2019) 167: 510-524 [PMID:30784883]
ChEMBL	Inhibition of BuChE in human serum using butyrylthiocholineiodide as substrate pre-incubated for 10 mins followed by substrate addition and measured for 6 mins by DTNB reagent based Ellman's method	B	5.92	pIC50	1210	nM	IC50	Eur J Med Chem (2021) 215: 113224-113224 [PMID:33582578]
ChEMBL	Inhibition of human serum BChE by Ellman's assay	B	6.03	pIC50	930	nM	IC50	J Med Chem (2008) 51: 3588-3598 [PMID:18517184]
ChEMBL	Inhibition of butyrylcholinesterase (unknown origin) using butyrylthiocholine chloride as substrate incubated for 10 mins followed by substrate addition measured after 20 mins by UV-Vis spectrophotometric analysis	B	6.89	pIC50	130	nM	IC50	Bioorg Med Chem (2017) 25: 100-106 [PMID:27780618]
ChEMBL	Inhibition of recombinant human BuChe using S-butyrylthiocholine iodide as substrate measured after 7 mins by Ellman's method	B	6.93	pIC50	117	nM	IC50	Eur J Med Chem (2021) 222: 113541-113541 [PMID:34116326]
ChEMBL	Inhibition of human BChE by Ellman's method	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem (2014) 22: 6163-6173 [PMID:25257911]
ChEMBL	Inhibition of human Cholinesterase	B	7.85	pIC50	14	nM	IC50	Bioorg Med Chem (2019) 27: 895-930 [PMID:30744931]
Cholinesterase in Equus caballus (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5763] [UniProtKB: P81908]
ChEMBL	Binding affinity to equine BChE in phosphate buffer at pH 8 assessed as equilibrium dissociation constant by SPR analysis	B	5.14	pKd	7200	nM	Kd	Eur J Med Chem (2024) 270: 116353-116353 [PMID:38579622]
ChEMBL	Non-competitive inhibition of horse serum BuChE using butyrylthiocoline iodide as substrate incubated for 20 mins by Lineweaver-Burk plot analysis	B	5.67	pKi	2140	nM	Ki	ACS Med Chem Lett (2016) 7: 470-475 [PMID:27190595]
ChEMBL	Inhibition of horse serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 10 mins by Ellman's method	B	4.45	pIC50	35400	nM	IC50	Eur J Med Chem (2022) 238: 114503-114503 [PMID:35689855]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	4.93	pIC50	11810	nM	IC50	Eur J Med Chem (2018) 147: 194-204 [PMID:29438888]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate incubated for 15 to 20 mins by Ellman's method	B	4.97	pIC50	10600	nM	IC50	Bioorg Med Chem Lett (2021) 52: 128306-128306 [PMID:34371131]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition by Ellman's method	B	4.98	pIC50	10380	nM	IC50	Eur J Med Chem (2019) 181: 111598-111598 [PMID:31415981]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by addition of substrate and measured after 20 mins by DTNB reagent based Ellman's method	B	4.98	pIC50	10380	nM	IC50	Eur J Med Chem (2020) 201: 112273-112273 [PMID:32569925]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by DTNB reagent based Ellman's method	B	5.01	pIC50	9830	nM	IC50	Eur J Med Chem (2023) 257: 115502-115502 [PMID:37224761]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	5.03	pIC50	9240	nM	IC50	Eur J Med Chem (2021) 223: 113735-113735 [PMID:34371367]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate measured for 6 mins by Ellman's method	B	5.06	pIC50	8730	nM	IC50	Bioorg Med Chem (2017) 25: 3980-3988 [PMID:28587871]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	5.06	pIC50	8710	nM	IC50	Eur J Med Chem (2019) 175: 2-19 [PMID:31055149]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman method	B	5.06	pIC50	8710	nM	IC50	Bioorg Med Chem (2019) 27: 3156-3166 [PMID:31176571]
ChEMBL	Inhibition of equine serum BuChE using DTNB as substrate incubated for 5 mins prior to substrate addition measured after 2 mins by Ellman's method	B	5.06	pIC50	8710	nM	IC50	Bioorg Med Chem (2015) 23: 6389-6403 [PMID:26337018]
ChEMBL	Inhibition of equine serum BuChE by Ellman's method	B	5.07	pIC50	8416	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127477-127477 [PMID:32781220]
ChEMBL	Inhibition of equine serum BChE using butyrylcholine chloride as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins under dark condition by Ellman's method	B	5.08	pIC50	8400	nM	IC50	Bioorg Med Chem (2019) 27: 4030-4040 [PMID:31378596]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured after 30 mins by Ellman's method	B	5.1	pIC50	7870	nM	IC50	Eur J Med Chem (2019) 177: 198-211 [PMID:31136894]
ChEMBL	Inhibition of horse serum butyrylcholinesterase using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	5.11	pIC50	7690	nM	IC50	Eur J Med Chem (2016) 107: 219-232 [PMID:26588065]
ChEMBL	Inhibition of equine serum BuchE using butyrylthiocholine iodide as substrate preincubated for 10 mins measured after 15 mins of substrate addition by Ellman's method	B	5.13	pIC50	7400	nM	IC50	J Med Chem (2011) 54: 8251-8270 [PMID:22023459]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 25 mins by Ellman's method	B	5.13	pIC50	7400	nM	IC50	Eur J Med Chem (2014) 80: 543-561 [PMID:24813882]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate measured for 2 mins by Ellman's method	B	5.13	pIC50	7400	nM	IC50	Eur J Med Chem (2016) 121: 40-46 [PMID:27214510]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 5 mins by DTNB-reagent based Ellman's method	B	5.13	pIC50	7390	nM	IC50	Eur J Med Chem (2022) 227: 113973-113973 [PMID:34752955]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate by spectrophotometer analysis	B	5.14	pIC50	7300	nM	IC50	Eur J Med Chem (2014) 82: 536-544 [PMID:24941128]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as substrate pre-incubated for 30 mins followed by substrate addition and measured after 5 mins by DTNB reagent based Ellman's method	B	5.14	pIC50	7290	nM	IC50	Eur J Med Chem (2024) 264: 116013-116013 [PMID:38052155]
ChEMBL	Inhibition of equine serum BuChE pre-incubated for 5 mins before butyrylthiocholine iodide substrate addition and measured after 15 mins by Ellman's method	B	5.14	pIC50	7200	nM	IC50	Bioorg Med Chem (2019) 27: 2914-2922 [PMID:31128990]
ChEMBL	Inhibition of equine serum BuChE pre-incubated for 5 mins before butyrylthiocholine iodide substrate by Ellman' method	B	5.15	pIC50	7000	nM	IC50	J Med Chem (2015) 58: 3172-3187 [PMID:25793650]
ChEMBL	Inhibition of horse serum BChE using butrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured for 10 mins by modified Ellman's method	B	5.19	pIC50	6500	nM	IC50	Bioorg Med Chem (2016) 24: 2352-2359 [PMID:27083471]
ChEMBL	Inhibition of equine serum BchE using S-butyryl thiocholine iodide as substrate incubated for 10 mins followed by substrate addition measured at 0 to 180 secs by Ellman's method	B	5.19	pIC50	6410	nM	IC50	Eur J Med Chem (2022) 234: 114210-114210 [PMID:35303485]
ChEMBL	Inhibition of equine BuChE using ACTI as substrate preincubated for 29 mins followed by substrate addition measured at 1 min intervals for 10 mins by Ellman's assay	B	5.23	pIC50	5910	nM	IC50	J Med Chem (2016) 59: 7683-7689 [PMID:27405689]
ChEMBL	Inhibition of equine serum BChE using S-butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured every minute for 10 mins by DTNB-reagent based Ellman's method	B	5.26	pIC50	5520	nM	IC50	Eur J Med Chem (2020) 207: 112761-112761 [PMID:32942070]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as substrate after 30 mins by Ellman's method	B	5.26	pIC50	5520	nM	IC50	Medchemcomm (2012) 3: 1098-1103
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide substrate by Ellman method based spectrophotometry	B	5.27	pIC50	5380	nM	IC50	Eur J Med Chem (2014) 84: 375-381 [PMID:25036795]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate after 2 mins by Ellman's method	B	5.27	pIC50	5380	nM	IC50	Eur J Med Chem (2013) 68: 260-269 [PMID:23988409]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 15 mins prior to substrate addition by Ellman's method	B	5.27	pIC50	5380	nM	IC50	Eur J Med Chem (2013) 70: 623-630 [PMID:24211638]
ChEMBL	Inhibition of BuChE in equine serum using Butyrylthiocholine iodide as substrate by Ellman's method	B	5.28	pIC50	5200	nM	IC50	Eur J Med Chem (2019) 179: 680-693 [PMID:31280020]
ChEMBL	Inhibition of equine serum BChE	B	5.29	pIC50	5130	nM	IC50	Bioorg Med Chem (2023) 80: 117178-117178 [PMID:36706609]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate by Ellman's method	B	5.31	pIC50	4900	nM	IC50	Medchemcomm (2019) 10: 1018-1026 [PMID:31304000]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5053-5059 [PMID:29033232]
ChEMBL	Inhibition of equine butyrylcholinesterase using butyrylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Eur J Med Chem (2019) 183: 111737-111737 [PMID:31581002]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine chloride as substrate incubated for 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Bioorg Med Chem Lett (2019) 29: 126625-126625 [PMID:31444085]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as susbtrate preincubated for 6 mins followed by substrate addition measured up to 3 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Eur J Med Chem (2019) 178: 726-739 [PMID:31229875]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate incubated for 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Eur J Med Chem (2019) 180: 238-252 [PMID:31310916]
ChEMBL	Inhibition of equine serum BChE by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Eur J Med Chem (2020) 192: 112180-112180 [PMID:32131034]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate measured after 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Eur J Med Chem (2017) 130: 379-392 [PMID:28279845]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate after 15 mins by Ellman's method	B	5.32	pIC50	4760	nM	IC50	Bioorg Med Chem (2017) 25: 3006-3017 [PMID:28487125]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine as substrate preincubated for 10 mins followed by substrate addition by spectrophotometric based Ellman's method	B	5.33	pIC50	4700	nM	IC50	Bioorg Med Chem (2022) 71: 116952-116952 [PMID:35930852]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate pretreated for 10 mins followed by substrate addition measured at 12 secs interval for 5 mins by Ellman's method	B	5.33	pIC50	4690	nM	IC50	Eur J Med Chem (2017) 130: 440-457 [PMID:28282613]
ChEMBL	Inhibition of equine serum BuChE using acetylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition by Ellman's method	B	5.33	pIC50	4690	nM	IC50	Eur J Med Chem (2018) 147: 48-65 [PMID:29421570]
ChEMBL	Inhibition of equine serum butyrylcholinesterase using butyrylthiocholine chloride as substrate incubated for 15 mins prior to substrate addition measured every 1 min by Ellman's assay	B	5.33	pIC50	4660	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4462-4466 [PMID:22633691]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	5.35	pIC50	4500	nM	IC50	Eur J Med Chem (2024) 266: 116131-116131 [PMID:38215587]
ChEMBL	Inhibition of equine serum BuChE using s-butyrylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 sec by Ellman's method	B	5.35	pIC50	4500	nM	IC50	Eur J Med Chem (2017) 133: 184-196 [PMID:28388521]
ChEMBL	Inhibition of horse serum BChE using butyrylthiocholine iodide as substrate preincubated for 5 mins by Ellman method	B	5.35	pIC50	4460	nM	IC50	Bioorg Med Chem (2015) 23: 2445-2457 [PMID:25868744]
ChEMBL	Inhibition of equine serum BuChE using ATCI and BTCI as substrate preincubated for 5 mins followed by substrate addition and measured after 3 mins by DTNB-reagent based Ellman's method	B	5.37	pIC50	4260	nM	IC50	Bioorg Med Chem (2023) 78: 117146-117146 [PMID:36580744]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition by DTNB reagent based Ellman's method	B	5.37	pIC50	4220	nM	IC50	Eur J Med Chem (2022) 242: 114630-114630 [PMID:35987018]
ChEMBL	Inhibition of equine serum butyrylcholinesterase preincubated for 5 mins followed by addition of S-butyrylthiocholine iodide as substrate measured after 60 to 120 mins by Ellmans method	B	5.38	pIC50	4170	nM	IC50	Medchemcomm (2016) 7: 990-998
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by spectrophotometric-based Ellman's method	B	5.42	pIC50	3790	nM	IC50	Bioorg Med Chem (2016) 24: 1528-1539 [PMID:26917219]
ChEMBL	Inhibition of equine serum BuChE using thiocholine iodide as substrate by Ellman's method	B	5.44	pIC50	3620	nM	IC50	Bioorg Med Chem Lett (2015) 25: 5212-5216 [PMID:26454504]
ChEMBL	Inhibition of equine BuChE using butyrylthiocholine iodide as substrate after 5 mins by DTNB method	B	5.44	pIC50	3600	nM	IC50	Bioorg Med Chem Lett (2012) 22: 4707-4712 [PMID:22704921]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 20 mins by Ellman's method	B	5.45	pIC50	3550	nM	IC50	Eur J Med Chem (2022) 229: 114095-114095 [PMID:34995924]
ChEMBL	Inhibition of equine serum BuChE using butylthiocholine iodine as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by DTNB reagent based Ellman's method	B	5.45	pIC50	3550	nM	IC50	Eur J Med Chem (2022) 242: 114689-114689 [PMID:36007469]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by Ellman's method	B	5.45	pIC50	3550	nM	IC50	Eur J Med Chem (2021) 212: 113124-113124 [PMID:33395623]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition and measured after 4 mins by Ellman's method	B	5.45	pIC50	3540	nM	IC50	Bioorg Med Chem (2021) 32: 115991-115991 [PMID:33440318]
ChEMBL	Inhibition of equine serum butyrylcholinesterase using butyrylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition by Ellman's method	B	5.46	pIC50	3490	nM	IC50	Bioorg Med Chem (2014) 22: 1355-1361 [PMID:24461494]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate pretreated for 5 mins followed by substrate addition by Ellman's method	B	5.47	pIC50	3400	nM	IC50	Eur J Med Chem (2018) 152: 600-614 [PMID:29763808]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition measured every 1 min by Ellman's method	B	5.47	pIC50	3365	nM	IC50	J Med Chem (2013) 56: 9089-9099 [PMID:24160297]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured every minute for 10 mins by Ellman's method	B	5.47	pIC50	3365	nM	IC50	Eur J Med Chem (2018) 145: 165-190 [PMID:29324339]
ChEMBL	Inhibition of equine BCHE preincubated for 6 mins followed by addition of S-butyrylcholine iodide as substrate by Ellman's method	B	5.47	pIC50	3360	nM	IC50	Bioorg Med Chem (2016) 24: 4324-4338 [PMID:27460699]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 2 mins by Ellman's method	B	5.47	pIC50	3360	nM	IC50	Bioorg Med Chem (2016) 24: 3829-3841 [PMID:27353888]
ChEMBL	Inhibition of equine serum butyrylcholine esterase incubated for 15 mins using S-butyrylthiocholine chloride substrate by colorimetric Ellman's method	B	5.48	pIC50	3310	nM	IC50	Eur J Med Chem (2015) 94: 195-210 [PMID:25768702]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 2 mins followed by substrate addition and measured after 2 mins by Ellman's method	B	5.49	pIC50	3200	nM	IC50	Bioorg Med Chem (2018) 26: 3812-3824 [PMID:29960728]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition and measured after 2 mins by Ellman's method	B	5.5	pIC50	3140	nM	IC50	Bioorg Med Chem (2016) 24: 2777-2788 [PMID:27157006]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate by Ellman's method	B	5.51	pIC50	3100	nM	IC50	Eur J Med Chem (2023) 251: 115253-115253 [PMID:36921526]
ChEMBL	Inhibition of equine serum BuChE using acetylthiocholine iodide as substrate preincubated for 15 mins followed by substrate addition by spectrophotometric analysis	B	5.51	pIC50	3060	nM	IC50	Bioorg Med Chem (2017) 25: 2946-2955 [PMID:28454848]
ChEMBL	Inhibition of equine serum BChE using S-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 180 secs by spectrophotometric analysis	B	5.54	pIC50	2890	nM	IC50	Eur J Med Chem (2017) 139: 68-83 [PMID:28800459]
ChEMBL	Inhibition of recombinant equine serum BuChE using butyryl thiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by spectrophotometry based Ellman's method	B	5.54	pIC50	2890	nM	IC50	Eur J Med Chem (2020) 198: 112257-112257 [PMID:32375073]
ChEMBL	Inhibition of equine serum BuChE using S-butylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by Ellman's method	B	5.54	pIC50	2870	nM	IC50	Eur J Med Chem (2017) 125: 853-864 [PMID:27744252]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	5.56	pIC50	2750	nM	IC50	Eur J Med Chem (2023) 259: 115695-115695 [PMID:37567058]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 2 mins by spectroscopy based Ellman's method	B	5.57	pIC50	2720	nM	IC50	Eur J Med Chem (2017) 136: 36-51 [PMID:28478343]
ChEMBL	Inhibition of horse serum butyrylcholine esterase in presence of acetylcholine substrate by chemiluminescent assay	B	5.57	pIC50	2700	nM	IC50	Eur J Med Chem (2008) 43: 657-661 [PMID:17624631]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured after 15 mins by Ellman's method	B	5.58	pIC50	2660	nM	IC50	Eur J Med Chem (2022) 229: 114044-114044 [PMID:34923430]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins by Ellman's method	B	5.59	pIC50	2600	nM	IC50	Bioorg Med Chem Lett (2020) 30: 126900-126900 [PMID:31882295]
ChEMBL	Inhibition of equine serum BuChE preincubated for 5 mins followed by addition of butyrylthiocholine iodide as substrate measured after 2 mins by Ellman's method	B	5.59	pIC50	2580	nM	IC50	Eur J Med Chem (2016) 119: 260-277 [PMID:27227482]
ChEMBL	Inhibition of horse serum BuChE using butyrylthiocholine as substrate by Ellman's method	B	5.6	pIC50	2500	nM	IC50	Eur J Med Chem (2014) 73: 56-72 [PMID:24378710]
ChEMBL	Inhibition of equine serum BuChE using s-butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured up to 3 mins by Ellman's method	B	5.61	pIC50	2480	nM	IC50	Eur J Med Chem (2016) 123: 282-297 [PMID:27484514]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition measured after 180 secs by Ellman's method	B	5.61	pIC50	2480	nM	IC50	Eur J Med Chem (2018) 157: 161-176 [PMID:30096650]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 6 mins followed by substrate addition and measured for 180 secs by Ellman's method	B	5.61	pIC50	2470	nM	IC50	RSC Med Chem (2020) 11: 225-233 [PMID:33479629]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured for 5 mins by spectrophotometric Ellman's method	B	5.64	pIC50	2310	nM	IC50	J Med Chem (2016) 59: 6791-6806 [PMID:27347731]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB based Ellman's method	B	5.64	pIC50	2300	nM	IC50	Eur J Med Chem (2024) 270: 116353-116353 [PMID:38579622]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine as substrate preincubated for 20 mins followed by substrate addition and measured upto 5 mins by Ellman's method	B	5.64	pIC50	2300	nM	IC50	Eur J Med Chem (2019) 177: 414-424 [PMID:31158754]
ChEMBL	Inhibition of equine serum BCHE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured for 5 mins by spectrophotometry based Ellman method	B	5.64	pIC50	2300	nM	IC50	Bioorg Med Chem Lett (2017) 27: 1179-1185 [PMID:28189420]
ChEMBL	Inhibition of horse serum BChE assessed as residual enzyme activity using butyrylthiocholine iodide as substrate by Ellman's colorimetric assay	B	5.65	pIC50	2250	nM	IC50	Bioorg Med Chem (2024) 110: 117829-117829 [PMID:39002183]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate incubated for 5 mins followed by substrate addition measured for 5 mins by Ellman's method	B	5.66	pIC50	2200	nM	IC50	Eur J Med Chem (2019) 174: 116-129 [PMID:31029943]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured after 30 mins by Ellman's method	B	5.67	pIC50	2160	nM	IC50	Bioorg Med Chem (2021) 46: 116385-116385 [PMID:34481338]
ChEMBL	Inhibition of horse serum BChE by Ellman's spectrophotometric assay	B	5.68	pIC50	2100	nM	IC50	J Med Chem (2022) 65: 3962-3977 [PMID:35195417]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate by Ellman's method	B	5.68	pIC50	2100	nM	IC50	Eur J Med Chem (2019) 161: 292-309 [PMID:30366255]
ChEMBL	Inhibition of equine BuChE by Ellman's method	B	5.69	pIC50	2057	nM	IC50	Eur J Med Chem (2021) 215: 113278-113278 [PMID:33662757]
ChEMBL	Inhibition of equine serum BuChE preincubated for 5 mins followed by substrate addition and measured after 5 mins using S-butyrylthiocholine iodide as substrate by Ellman's method	B	5.7	pIC50	1980	nM	IC50	Eur J Med Chem (2021) 215: 113278-113278 [PMID:33662757]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate incubated for 5 mins by Ellman's method	B	5.72	pIC50	1911	nM	IC50	Bioorg Med Chem (2015) 23: 1135-1148 [PMID:25624107]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition by DTNB reagent based Ellman's method	B	5.72	pIC50	1900	nM	IC50	Eur J Med Chem (2023) 250: 115169-115169 [PMID:36753881]
ChEMBL	Inhibition of equine butyrylcholinesterase using butyrylthiocholine as substrate incubated for 20 mins prior to substrate addition measured after 3 mins by Ellman's method	B	5.72	pIC50	1900	nM	IC50	Bioorg Med Chem (2013) 21: 146-152 [PMID:23199476]
ChEMBL	Inhibition of equine serum BuChE using BTC as substrate preincubated with enzyme for 5 mins followed by substrate addition for 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2021) 225: 113792-113792 [PMID:34530376]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine as substrate incubated for 5 mins followed by substrate addition and measured after 5 mins by spectrophotometric based Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2021) 225: 113783-113783 [PMID:34461507]
ChEMBL	Inhibition of equine serum BuChE preincubated for 5 mins followed by butyrylthiocholine iodide substrate addition measured after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2016) 124: 63-81 [PMID:27560283]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2017) 125: 676-695 [PMID:27721153]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2021) 218: 113397-113397 [PMID:33838585]
ChEMBL	Inhibition of horse serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by DTNB-reagent based Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2023) 249: 115135-115135 [PMID:36696766]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 5 mins prior to substrate addition measured after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2020) 185: 111785-111785 [PMID:31669851]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine chloride as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins by Ellmans method	B	5.74	pIC50	1830	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4140-4145 [PMID:27445168]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine chloride as substrate pre-incubated for 5 mins followed by substrate addition and measured after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2020) 187: 111916-111916 [PMID:31812794]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by DTNB reagent based Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2023) 261: 115832-115832 [PMID:37837674]
ChEMBL	Inhibition of equine serum BuChE by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Bioorg Med Chem (2023) 88-89: 117333-117333 [PMID:37236021]
ChEMBL	Inhibition of equine serum BuChE after 5 mins by Ellman's method	B	5.74	pIC50	1830	nM	IC50	Eur J Med Chem (2015) 92: 738-749 [PMID:25621991]
ChEMBL	Inhibition of BuChE in horse serum pre-incubated for 5 mins before addition of butyrylthiocholine iodide substrate by Ellman's assay	B	5.74	pIC50	1830	nM	IC50	Bioorg Med Chem (2015) 23: 1629-1637 [PMID:25707322]
ChEMBL	Inhibition of BuChE in equine serum using butyrylthiocholine iodide as substrate preincubated with enzyme for 10 mins prior to substrate challenge measured after 15 mins by Ellman's method	B	5.74	pIC50	1820	nM	IC50	Bioorg Med Chem (2014) 22: 2496-2507 [PMID:24657052]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine as substrate by Ellman's method	B	5.78	pIC50	1670	nM	IC50	Eur J Med Chem (2024) 276: 116592-116592 [PMID:39013357]
ChEMBL	Inhibition of horse serum BuChE using acetylthiocholine as substrate after 60 mins by FRET assay	B	5.87	pIC50	1340	nM	IC50	Bioorg Med Chem Lett (2018) 28: 2910-2913 [PMID:30017317]
ChEMBL	Inhibition of recombinant equine BChE by Ellman's method	B	5.89	pIC50	1300	nM	IC50	Bioorg Med Chem (2021) 46: 116355-116355 [PMID:34391122]
ChEMBL	Inhibition of horse serum BuChE using butyrylthiocoline iodide as substrate incubated for 20 mins by Ellman method	B	5.9	pIC50	1270	nM	IC50	ACS Med Chem Lett (2016) 7: 470-475 [PMID:27190595]
ChEMBL	Inhibition of equine serum BuChE using BTCI as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by Ellman assay	B	5.9	pIC50	1260	nM	IC50	J Med Chem (2016) 59: 5823-5846 [PMID:27253679]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition and measured every min for 10 mins by DTNB based Ellman's assay	B	5.9	pIC50	1260	nM	IC50	Bioorg Med Chem (2024) 101: 117649-117649 [PMID:38401458]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured every 1 min for 20 mins by spectrophotometric analysis	B	6.03	pIC50	941	nM	IC50	Eur J Med Chem (2023) 259: 115670-115670 [PMID:37515920]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured for 1 min by DTNB reagent-based spectrophotometric analysis	B	6.03	pIC50	941	nM	IC50	Eur J Med Chem (2019) 182: 111613-111613 [PMID:31437780]
ChEMBL	Inhibition of equine serum BuChE by Ellman's method	B	6.08	pIC50	840	nM	IC50	J Med Chem (2010) 53: 5129-5143 [PMID:20575555]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 25 mins by Ellman's method	B	6.08	pIC50	840	nM	IC50	Eur J Med Chem (2014) 75: 82-95 [PMID:24530494]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 15 mins by Ellman's method	B	6.27	pIC50	540	nM	IC50	Bioorg Med Chem Lett (2015) 25: 5576-5582 [PMID:26514744]
ChEMBL	Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured for 5 mins by Ellman's method	B	6.46	pIC50	350	nM	IC50	Bioorg Med Chem (2018) 26: 4952-4962 [PMID:30190181]
ChEMBL	Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 10 mins followed by substrate addition measured for 5 mins by Ellman's method	B	6.46	pIC50	350	nM	IC50	Eur J Med Chem (2018) 155: 49-60 [PMID:29857276]
ChEMBL	Inhibition of horse serum BuChE using acetylthiocholine as substrate by UV-visible spectrophotometric Ellman's method	B	6.64	pIC50	231	nM	IC50	Bioorg Med Chem Lett (2018) 28: 2910-2913 [PMID:30017317]
ChEMBL	Inhibition of horse serum BChE using butyrylthiocholine iodide as substrate pretreated for 10 mins followed by substrate addition measured after 20 mins by Ellman's method	B	6.89	pIC50	130	nM	IC50	Bioorg Med Chem (2018) 26: 3696-3706 [PMID:29886083]
ChEMBL	Inhibition of equine serum BuChE using S-butyrylthiocholine iodide as substrate preincubated for 6 mins followed by substrate addition measured up to 180 secs by Ellman's method	B	8.34	pIC50	4.6	nM	IC50	Eur J Med Chem (2017) 139: 48-59 [PMID:28797883]
butyrylcholinesterase/Cholinesterase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2528] [GtoPdb: 2471] [UniProtKB: Q03311]
ChEMBL	Inhibition of BuChE in mouse brain homogenate using butyrylthiocholine as substrate preincubated for 300 secs followed by substrate addition by Ellman's method	B	5.15	pIC50	7138	nM	IC50	J Med Chem (2018) 61: 119-139 [PMID:29227101]
ChEMBL	Inhibition of BuChE in mouse serum using butyrylthiocholine iodide as substrate incubated for 20 mins by Ellman's method	B	5.7	pIC50	1992	nM	IC50	Eur J Med Chem (2022) 229: 114045-114045 [PMID:34922191]
glycogen synthase kinase 3 beta/Glycogen synthase kinase-3 beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL262] [GtoPdb: 2030] [UniProtKB: P49841]
ChEMBL	Inhibition of human recombinant GSK-3beta using prephosphorylated polypeptide as substrate incubated for 30 mins in presence of ATP by Kinase-Glo luminescence assay	B	4	pIC50	>100000	nM	IC50	J Med Chem (2021) 64: 7483-7506 [PMID:34024109]
ChEMBL	Inhibition of human recombinant N-terminal His-tagged GSK3beta expressed in Escherichia coli using RRRPASVPPSPSLS RHS(pS)HQRR as substrate incubated for 30 mins in presence of ATP by Kinase-Glo reagent based luminescence assay	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 223: 113663-113663 [PMID:34198150]
ChEMBL	Inhibition of recombinant human N-terminal His6-tagged GSK3beta H350L mutant expressed in baculovirus infected Sf21 cells using prephosphorylated polypeptide as substrate incubated for 1 min followed by substrate addition and measured after 30 mins by Kinase-Glo luminescence assay	B	6	pIC50	>1000	nM	IC50	Bioorg Med Chem (2021) 30: 115940-115940 [PMID:33340937]
H3 receptor/Histamine H3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1]
ChEMBL	Inhibition of histamine H3 receptor	B	6.46	pIC50	350	nM	IC50	Bioorg Med Chem (2008) 16: 2968-2973 [PMID:18249544]
carboxylesterase 1/Liver carboxylesterase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141]
ChEMBL	Inhibition of human carboxylesterase 1 using o-nitrophenylacetate as substrate by spectrophotometry analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem (2016) 24: 1050-1062 [PMID:26827140]
microtubule affinity regulating kinase 4/MAP/microtubule affinity-regulating kinase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5754] [GtoPdb: 2100] [UniProtKB: Q96L34]
ChEMBL	Inhibition of human MARK4 expressed in Escherichia coli preincubated for 1 hr followed by ATP addition and measured after 30 mins by colorimetric assay	B	5.28	pIC50	5300	nM	IC50	RSC Med Chem (2024) 15: 1942-1958 [PMID:38911173]
sigma non-opioid intracellular receptor 1/Sigma non-opioid intracellular receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720]
ChEMBL	Displacement of [3H]DTG from sigma 1 receptor (unknown origin)	B	7.84	pKi	14.6	nM	Ki	Bioorg Med Chem (2013) 21: 2764-2771 [PMID:23582449]
ChEMBL	Inhibition of [3H]DTG binding to sigma 1 receptor (unknown origin)	B	7.84	pKi	14.6	nM	Ki	J Med Chem (2020) 63: 15187-15217 [PMID:33111525]
Sigma non-opioid intracellular receptor 1 in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4153] [UniProtKB: Q60492]
ChEMBL	Displacement of [3H]DTG from S1R in P2 fraction of Hartley guinea pig whole brain after 90 mins by liquid scintillation counting method	B	7.84	pKi	14.6	nM	Ki	Eur J Med Chem (2018) 158: 353-370 [PMID:30223122]
ChEMBL	Displacement of [3H]-(+)-pentazocine from sigma1 receptor in guinea pig brain membrane measured after 120 mins by scintillation counting method	B	7.84	pKi	14.6	nM	Ki	J Med Chem (2022) 65: 12292-12318 [PMID:36084304]
Kv11.1/Voltage-gated inwardly rectifying potassium channel KCNH2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Inhibition of hERG expressed in CHO-K1 cells assessed as reduction in thallium influx incubated for 30 minutes by FLIPR	B	5.06	pIC50	8718	nM	IC50	Eur J Med Chem (2021) 226: 113827-113827 [PMID:34530383]
ChEMBL	Inhibition of human ERG	B	5.7	pIC50	1995.26	nM	IC50	Eur J Med Chem (2011) 46: 618-630 [PMID:21185626]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]