ChEMBL ligand: CHEMBL714 (Accuneb, Aerolin, Aerolin 400, Aerolin auto, Airomir, Airsalb, Albuterol, Asmasal clickhaler, Asmasal spacehaler, Asmaven, Asmavent, Cobutolin, Combivent, Combivent udvs, Duoneb, Etinoline, Ipramol steri-neb, Kentamol, Libetist, Maxivent, NSC-757417, Proair Hfa, Proventil, Proventil Hfa, PROVENTIL-HFA, Rimasal, Salamol e-breathe, Salapin, Salbulin novolizer, Salbutamol, Salbutamolum, Salbuvent, Salipraneb, SCH 13949W, SCH-13949W, Steri-neb salamol, Ventmax sr, Ventodisks, Ventolin, Ventolin cr, Ventoline, Ventolin e-breathe, Ventolin Hfa, Volmax)
β1-adrenoceptor/Beta-1 adrenergic receptor in Human [ChEMBL: CHEMBL213] [GtoPdb: 28] [UniProtKB: P08588]
β2-adrenoceptor/Beta-2 adrenergic receptor in Human [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550] Beta-2 adrenergic receptor in Bovine [ChEMBL: CHEMBL3373] [UniProtKB: Q28044] Beta-2 adrenergic receptor in Guinea pig [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4]
GroEL/GroES in Escherichia coli [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6]
HSP60/HSP10 in Human [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604]

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
β1-adrenoceptor/Beta-1 adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL213] [GtoPdb: 28] [UniProtKB: P08588]
ChEMBL	Agonist activity at human beta1 adrenoceptor expressed in CHO cells assessed as increase in intracellular cAMP level incubated for 1 hr by alphascreen technology	F	5.93	pEC50	1170	nM	EC50	J Med Chem (2020) 63: 15218-15242 [PMID:33213146]
ChEMBL	Agonist activity at beta1 adrenoceptor (unknown origin) expressed in HEK293 cells assessed as cAMP accumulation incubated for 60 mins by microplate reader analysis	F	8.82	pEC50	1.53	nM	EC50	J Med Chem (2022) 65: 5514-5527 [PMID:35360904]
β2-adrenoceptor/Beta-2 adrenergic receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL210] [GtoPdb: 29] [UniProtKB: P07550]
ChEMBL	Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes assessed as intrinsic Kd by liquid scintillation counting	B	5.85	pKd	1400	nM	Kd	J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL	Displacement of [3H]DHA from inactive/G protein-uncoupled human beta2-AR expressed in CHO cell membranes by liquid scintillation counting	B	6.66	pKd	220	nM	Kd	J Med Chem (2016) 59: 5780-5789 [PMID:27239696]
ChEMBL	Displacement of [125I]cyanopindolol from human recombinant beta2 adrenergic receptor expressed in CHO cells by filtration assay	B	5.74	pKi	1828	nM	Ki	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
GtoPdb	-	-	6.1	pKi	-	-	-	Mol Pharmacol (1999) 56: 875-85 [PMID:10531390]; Br J Pharmacol (2005) 144: 317-22 [PMID:15655528]; Br J Pharmacol (2010) 160: 1048-61 [PMID:20590599]; Bioorg Med Chem (2012) 20: 933-41 [PMID:22182578]; Br J Pharmacol (2022) 179: 4692-4708 [PMID:35732075]; Mol Pharmacol (2015) 87: 103-20 [PMID:25324048]
ChEMBL	DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177)	B	6.25	pKi	557	nM	Ki	DrugMatrix in vitro pharmacology data
ChEMBL	Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting	B	6.29	pKi	510	nM	Ki	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	Displacement of [3H]dihydroalprenolol from beta2-adrenoceptor after 90 mins by liquid scintillation counting	B	5.7	pIC50	1995.26	nM	IC50	Bioorg Med Chem Lett (2012) 22: 1213-1218 [PMID:22178551]
ChEMBL	Displacement of [3H]CGP1217 from human recombinant adrenergic beta2 receptor transfected in insect Sf9 cells by scintillation counting	B	6.01	pIC50	980	nM	IC50	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	DRUGMATRIX: Adrenergic beta2 radioligand binding (ligand: [3H] CGP-12177)	B	6.09	pIC50	810.2	nM	IC50	DrugMatrix in vitro pharmacology data
ChEMBL	Agonist activity at human beta2 adrenoceptor expressed in CHO cells assessed as increase in intracellular cAMP level incubated for 1 hr by alphascreen technology	F	6.6	pEC50	251	nM	EC50	J Med Chem (2020) 63: 15218-15242 [PMID:33213146]
ChEMBL	Agonist activity at human adrenergic beta2 receptor expressed in H292 cells assessed as intracellular cAMP accumulation after 1 hr	F	6.7	pEC50	199.53	nM	EC50	Bioorg Med Chem Lett (2011) 21: 4027-4031 [PMID:21652207]
ChEMBL	Agonist activity at human beta2 adrenergic receptor assessed as increase in cAMP level by whole cell assay	F	7.17	pEC50	68	nM	EC50	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL	Agonist activity at recombinant human beta2-adrenoceptor expressed in whole cells assessed as cAMP accumulation by homogeneous radioimmunoassay	F	7.6	pEC50	25.12	nM	EC50	Bioorg Med Chem Lett (2012) 22: 1213-1218 [PMID:22178551]
GtoPdb	-	-	7.7	pEC50	-	-	-	Br J Pharmacol (2010) 160: 1048-61 [PMID:20590599]; Br J Pharmacol (2022) 179: 4692-4708 [PMID:35732075]
ChEMBL	Agonist activity at beta2 adrenoceptor (unknown origin) expressed in HEK293 cells assessed as cAMP accumulation incubated for 60 mins by microplate reader analysis	F	9.96	pEC50	0.11	nM	EC50	J Med Chem (2022) 65: 5514-5527 [PMID:35360904]
Beta-2 adrenergic receptor in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3373] [UniProtKB: Q28044]
ChEMBL	Apparent binding affinity constant to beta-2 adrenergic receptor determined using [3H]DHA	B	6.24	pKd	0.57	uM	KD app	J Med Chem (1986) 29: 549-554 [PMID:2870189]
ChEMBL	Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA)	B	6.28	pKd	520	nM	Kd	J Med Chem (1985) 28: 1328-1334 [PMID:2993621]
ChEMBL	Affinity for cow beta-2 adrenergic receptor by measuring displacement (-)-[3H]dihydroalprenolol (DHA)	B	6.58	pKd	6.58	-	-Log KD	J Med Chem (1985) 28: 1328-1334 [PMID:2993621]
Beta-2 adrenergic receptor in Guinea pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5414] [UniProtKB: Q8K4Z4]
ChEMBL	Binding affinity to adrenergic beta2 receptor in Hartley guinea pig tracheal ring	B	5.94	pKd	1160	nM	Kd	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction	B	6.61	pKd	245.47	nM	Kd	Bioorg Med Chem Lett (2015) 25: 820-825 [PMID:25592716]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring at basal tone assessed as bronchorelaxation activity	B	6.96	pKd	109.65	nM	Kd	Bioorg Med Chem Lett (2015) 25: 820-825 [PMID:25592716]
ChEMBL	Agonist activity at beta2 adrenergic receptor in guinea pig tracheal strip assessed as inhibition of electrically-induced bronchocontractile response after 30 mins	F	7.77	pIC50	17	nM	IC50	J Med Chem (2010) 53: 3675-3684 [PMID:20402514]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against carbachol-induced contraction	F	6.57	pEC50	268	nM	EC50	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
ChEMBL	Agonist activity at adrenergic beta2 receptor in guinea pig trachea assessed as relaxation of carbachol-induced tissue contraction	F	8.04	pEC50	9.1	nM	EC50	J Med Chem (2009) 52: 1773-1777 [PMID:19245211]
ChEMBL	Agonist activity at adrenergic beta2 receptor in Hartley guinea pig tracheal ring assessed as bronchorelaxation activity against histamine-induced contraction	F	9.34	pEC50	0.45	nM	EC50	Eur J Med Chem (2009) 44: 2840-2846 [PMID:19168263]
GroEL/GroES in Escherichia coli (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106139] [UniProtKB: Q548M1, Q7BGE6]
ChEMBL	Inhibition of Escherichia coli GroEL expressed in Escherichia coliDH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured soluble pig heart MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 20 to 40 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]
HSP60/HSP10 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4106131] [UniProtKB: P10809, P61604]
ChEMBL	Inhibition of human N-terminal octa-His-tagged HSP60 expressed in Escherichia coli Rosetta(DE3) pLysS/human HSP10 expressed in Escherichia coli Rosetta(DE3) assessed as reduction in HSP60/HSP10-mediated denatured MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 40 to 60 mins by malachite green dye based spectrometric analysis	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2019) 29: 1106-1112 [PMID:30852084]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]