ChEMBL ligand: CHEMBL177577 (OL-135)
carboxylesterase 1/Acyl coenzyme A:cholesterol acyltransferase in Human [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141] There should be some charts here, you may need to enable JavaScript!
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519] Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914] Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612] There should be some charts here, you may need to enable JavaScript!
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7] There should be some charts here, you may need to enable JavaScript!
Neutral cholesterol ester hydrolase 1 in Human [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
carboxylesterase 1/Acyl coenzyme A:cholesterol acyltransferase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141]
ChEMBL	Inhibitory concentration against Triacylglycerol hydrolase	B	6.22	pIC50	600	nM	IC50	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibitory concentration of triacylgylcerol hydrolase using FP-Rh radioligand	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	>600	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519]
ChEMBL	Inhibition of FAAH	B	5.33	pKi	4700	nM	Ki	J Med Chem (2008) 51: 7327-7343 [PMID:18983142]
ChEMBL	Inhibition of human recombinant FAAH	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2008) 16: 1562-1595 [PMID:18053726]
ChEMBL	Inhibitory constant determined against recombinant Fatty-acid amide hydrolase from human expressed in COS-7 cells	B	8.05	pKi	9	nM	Ki	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibition of human recombinant FAAH expressed in african green monkey COS7 cells	B	8.05	pKi	9	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli by [14C]oleamide breakdown	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2011) 21: 4674-4685 [PMID:21764305]
ChEMBL	Inhibition of FAAH at pH 9	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 5847-5850 [PMID:18644727]
ChEMBL	Inhibition of FAAH	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of human recombinant FAAH	B	6.2	pIC50	628	nM	IC50	J Med Chem (2012) 55: 6898-6915 [PMID:22779702]
ChEMBL	Inhibition of FAAH	B	6.64	pIC50	230	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of human FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.92	pIC50	119	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
GtoPdb	-	-	7.4	pIC50	39.8	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]
ChEMBL	Reversible inhibition of human recombinant FAAH using N-arachidonyl-7-amino-4-methylcoumarin as substrate preincubated for 20 mins before substrate addition by fluorescence assay	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem (2013) 21: 5188-5197 [PMID:23891163]
ChEMBL	Inhibition of human FAAH	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2109-2113 [PMID:18289847]
ChEMBL	Inhibitory concentration of fatty acid amide hydrolase using FP-Rh radioligand	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of human transmembrane domain deficient FFAH expressed in Escherichia coli using AAMCA as substrate incubated for 1 min measured for 50 mins by fluorescence assay	B	8.28	pIC50	5.2	nM	IC50	Bioorg Med Chem Lett (2019) 29: 238-243 [PMID:30503633]
ChEMBL	Reversible inhibition of human recombinant FAAH assessed as hydrolysis of [3H]-AEA after 10 mins by liquid scintillation counting	B	8.33	pIC50	4.7	nM	IC50	J Med Chem (2011) 54: 6812-6823 [PMID:21899370]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli by [14C]oleamide breakdown	B	8.7	pIC50	2	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibitory concentration against Fatty-acid amide hydrolase	B	8.7	pIC50	2	nM	IC50	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibition of MBP-fused human recombinant FAAH with truncated N-terminal transmembrane domain expressed in Escherichia coli T7 using D-AMC substrate measured over 40 mins by fluorescence based assay	B	9.4	pIC50	0.4	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2965-2973 [PMID:27130358]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914]
ChEMBL	Inhibitor affinity towards enzymes of class serine hydrolase was determined using biotin or fluorescent as radioligand (FP-biotin or FP-Rh)	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of mouse FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2014) 22: 2763-2770 [PMID:24690529]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli using [14C]oleamide as substrate assessed as oleic acid formation by Dixon plot analysis	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2014) 22: 2763-2770 [PMID:24690529]
ChEMBL	Binding affinity against purified rat Fatty-acid amide hydrolase (FAAH) expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2005) 15: 103-106 [PMID:15582420]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2007) 50: 3359-3368 [PMID:17559203]
ChEMBL	Inhibition of rat FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2008) 16: 1562-1595 [PMID:18053726]
ChEMBL	Inhibition of rat FAAH preincubated for 20 mins	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 4838-4843 [PMID:18693015]
ChEMBL	Inhibition of rat cortex FAAH by [3H]anandamide carbon filtration assay	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of rat FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat FAAH expressed in Escherichia coli at pH 9.0	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2010) 53: 230-240 [PMID:19924997]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2012) 20: 1100-1112 [PMID:22196515]
ChEMBL	Inhibitory constant determined against recombinant Fatty-acid amide hydrolase from rat expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 1 hr by Dixon plot analysis	B	8.38	pKi	4.2	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 3 hrs by Dixon plot analysis	B	8.42	pKi	3.8	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 6 hrs by Dixon plot analysis	B	8.52	pKi	3	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Inhibition of rat FAAH incubated 3 hrs prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.51	pIC50	306	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of rat FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.95	pIC50	112	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.7	pIC50	2	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of rat FAAH	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7]
ChEMBL	Inhibition of recombinant human N-terminal FLAG-tagged/C-terminal Myc-His6 tagged FAAH2 (32 to 579 residues) expressed in COS7 cell membranes	B	7.87	pIC50	13.4	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
GtoPdb	-	-	8.4	pIC50	4	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]; Anesth Analg (2009) 108: 316-29 [PMID:19095868]
Neutral cholesterol ester hydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of KIAA1363 hydrolase	B	4	pIC50	>100000	nM	IC50	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]