ChEMBL ligand: CHEMBL177577 (OL-135)
carboxylesterase 1/Acyl coenzyme A:cholesterol acyltransferase in Human [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141] There should be some charts here, you may need to enable JavaScript!
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519] Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914] Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612] There should be some charts here, you may need to enable JavaScript!
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7] There should be some charts here, you may need to enable JavaScript!
Neutral cholesterol ester hydrolase 1 in Human [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
carboxylesterase 1/Acyl coenzyme A:cholesterol acyltransferase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2265] [GtoPdb: 2592] [UniProtKB: P23141]
ChEMBL	Inhibitory concentration against Triacylglycerol hydrolase	B	6.22	pIC50	600	nM	IC50	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibitory concentration of triacylgylcerol hydrolase using FP-Rh radioligand	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	>600	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of TGH	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2243] [GtoPdb: 1400] [UniProtKB: O00519]
ChEMBL	Inhibition of FAAH	B	5.33	pKi	4700	nM	Ki	J Med Chem (2008) 51: 7327-7343 [PMID:18983142]
ChEMBL	Inhibition of human recombinant FAAH	B	8.05	pKi	9	nM	Ki	Bioorg Med Chem (2008) 16: 1562-1595 [PMID:18053726]
ChEMBL	Inhibitory constant determined against recombinant Fatty-acid amide hydrolase from human expressed in COS-7 cells	B	8.05	pKi	9	nM	Ki	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibition of human recombinant FAAH expressed in african green monkey COS7 cells	B	8.05	pKi	9	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli by [14C]oleamide breakdown	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2011) 21: 4674-4685 [PMID:21764305]
ChEMBL	Inhibition of FAAH at pH 9	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 5847-5850 [PMID:18644727]
ChEMBL	Inhibition of FAAH	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of human recombinant FAAH	B	6.2	pIC50	628	nM	IC50	J Med Chem (2012) 55: 6898-6915 [PMID:22779702]
ChEMBL	Inhibition of FAAH	B	6.64	pIC50	230	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of human FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.92	pIC50	119	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
GtoPdb	-	-	7.4	pIC50	39.8	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]
ChEMBL	Reversible inhibition of human recombinant FAAH using N-arachidonyl-7-amino-4-methylcoumarin as substrate preincubated for 20 mins before substrate addition by fluorescence assay	B	7.48	pIC50	33	nM	IC50	Bioorg Med Chem (2013) 21: 5188-5197 [PMID:23891163]
ChEMBL	Inhibition of human FAAH	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2109-2113 [PMID:18289847]
ChEMBL	Inhibitory concentration of fatty acid amide hydrolase using FP-Rh radioligand	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of human transmembrane domain deficient FFAH expressed in Escherichia coli using AAMCA as substrate incubated for 1 min measured for 50 mins by fluorescence assay	B	8.28	pIC50	5.2	nM	IC50	Bioorg Med Chem Lett (2019) 29: 238-243 [PMID:30503633]
ChEMBL	Reversible inhibition of human recombinant FAAH assessed as hydrolysis of [3H]-AEA after 10 mins by liquid scintillation counting	B	8.33	pIC50	4.7	nM	IC50	J Med Chem (2011) 54: 6812-6823 [PMID:21899370]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli by [14C]oleamide breakdown	B	8.7	pIC50	2	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibitory concentration against Fatty-acid amide hydrolase	B	8.7	pIC50	2	nM	IC50	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Inhibition of MBP-fused human recombinant FAAH with truncated N-terminal transmembrane domain expressed in Escherichia coli T7 using D-AMC substrate measured over 40 mins by fluorescence based assay	B	9.4	pIC50	0.4	nM	IC50	Bioorg Med Chem Lett (2016) 26: 2965-2973 [PMID:27130358]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3455] [GtoPdb: 1400] [UniProtKB: O08914]
ChEMBL	Inhibitor affinity towards enzymes of class serine hydrolase was determined using biotin or fluorescent as radioligand (FP-biotin or FP-Rh)	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2005) 15: 1423-1428 [PMID:15713400]
ChEMBL	Inhibition of mouse FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2014) 22: 2763-2770 [PMID:24690529]
Fatty acid amide hydrolase/Anandamide amidohydrolase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3229] [GtoPdb: 1400] [UniProtKB: P97612]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli using [14C]oleamide as substrate assessed as oleic acid formation by Dixon plot analysis	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2014) 22: 2763-2770 [PMID:24690529]
ChEMBL	Binding affinity against purified rat Fatty-acid amide hydrolase (FAAH) expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2005) 15: 103-106 [PMID:15582420]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2007) 50: 3359-3368 [PMID:17559203]
ChEMBL	Inhibition of rat FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2008) 16: 1562-1595 [PMID:18053726]
ChEMBL	Inhibition of rat FAAH preincubated for 20 mins	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 4838-4843 [PMID:18693015]
ChEMBL	Inhibition of rat cortex FAAH by [3H]anandamide carbon filtration assay	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2009) 52: 170-180 [PMID:19072118]
ChEMBL	Inhibition of rat FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat FAAH expressed in Escherichia coli at pH 9.0	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of rat FAAH	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2010) 53: 230-240 [PMID:19924997]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	Bioorg Med Chem (2012) 20: 1100-1112 [PMID:22196515]
ChEMBL	Inhibitory constant determined against recombinant Fatty-acid amide hydrolase from rat expressed in Escherichia coli	B	8.33	pKi	4.7	nM	Ki	J Med Chem (2005) 48: 1849-1856 [PMID:15771430]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 1 hr by Dixon plot analysis	B	8.38	pKi	4.2	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 3 hrs by Dixon plot analysis	B	8.42	pKi	3.8	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Time-dependent inhibition of recombinant rat FAAH expressed in Escherichia coli assessed as breakdown of [14C]-oleamide preincubated for 6 hrs by Dixon plot analysis	B	8.52	pKi	3	nM	Ki	J Med Chem (2014) 57: 1079-1089 [PMID:24456116]
ChEMBL	Inhibition of rat FAAH incubated 3 hrs prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.51	pIC50	306	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of rat FAAH incubated 1 hr prior to addition of arachidonyl-amino-methyl-coumarin amide	B	6.95	pIC50	112	nM	IC50	Bioorg Med Chem Lett (2009) 19: 4241-4244 [PMID:19515560]
ChEMBL	Inhibition of rat recombinant FAAH expressed in Escherichia coli	B	8.7	pIC50	2	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of rat FAAH	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]
Fatty acid amide hydrolase-2/Fatty-acid amide hydrolase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1628475] [GtoPdb: 1401] [UniProtKB: Q6GMR7]
ChEMBL	Inhibition of recombinant human N-terminal FLAG-tagged/C-terminal Myc-His6 tagged FAAH2 (32 to 579 residues) expressed in COS7 cell membranes	B	7.87	pIC50	13.4	nM	IC50	J Med Chem (2017) 60: 4-46 [PMID:27766867]
GtoPdb	-	-	8.4	pIC50	4	nM	IC50	J Biol Chem (2006) 281: 36569-78 [PMID:17015445]; Anesth Analg (2009) 108: 316-29 [PMID:19095868]
Neutral cholesterol ester hydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5048] [UniProtKB: Q6PIU2]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	J Med Chem (2007) 50: 1058-1068 [PMID:17279740]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	J Med Chem (2008) 51: 937-947 [PMID:18247553]
ChEMBL	Inhibition of KIAA1363 hydrolase	B	4	pIC50	>100000	nM	IC50	J Med Chem (2008) 51: 4392-4403 [PMID:18630870]
ChEMBL	Inhibition of KIAA1363	B	4	pIC50	>100000	nM	IC50	Bioorg Med Chem Lett (2008) 18: 5842-5846 [PMID:18639454]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]