ChEMBL ligand: CHEMBL88147 (MRS-1220)
A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] A1 receptor/Adenosine A1 receptor in Rat [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099] There should be some charts here, you may need to enable JavaScript!
A2A receptor/Adenosine A2a receptor in Human [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274] A2A receptor/Adenosine A2a receptor in Rat [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543] There should be some charts here, you may need to enable JavaScript!
A3 receptor/Adenosine A3 receptor in Human [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8] A3 receptor/Adenosine A3 receptor in Rat [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647] There should be some charts here, you may need to enable JavaScript!
5-HT1D receptor/Serotonin 1d (5-HT1d) receptor in Human [ChEMBL: CHEMBL1983] [GtoPdb: 3] [UniProtKB: P28221] There should be some charts here, you may need to enable JavaScript!
A2B receptor in Human [GtoPdb: 20] [UniProtKB: P29275] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
ChEMBL	Binding affinity to Nluc-A1R (unknown origin) expressed in HEK293 cells by nanoBRET assay	B	7.8	pKd	15.85	nM	Kd	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Displacement of [3H]CCPA from human recombinant adenosine A1 receptor expressed in CHO cells after 60 mins by gamma counter	B	6.64	pKi	231	nM	Ki	J Med Chem (2012) 55: 5676-5703 [PMID:22468757]
ChEMBL	Binding affinity to wild type A1R (unknown origin) expressed in CHO-K1 cells assessed as equilibrium binding affinity constant by nanoBRET assay	B	7.29	pKi	51.29	nM	Ki	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Displacement of CA200645 from NLuc human A1 receptor expressed in HEK293 cells assessed as inhibition constant by NanoBRET binding assay	B	7.29	pKi	51.29	nM	Ki	ACS Med Chem Lett (2022) 13: 923-934 [PMID:35707146]
ChEMBL	Antagonist activity at wild type A1R (unknown origin) expressed in CHO-K1 cells assessed as inhibition of NECA reduced forskolin stimulated cAMP accumulation incubated for 30 mins	F	7.32	pIC50	47.86	nM	IC50	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Antagonist activity at human wild type A1R expressed in CHO-K1 cells assessed as inhibition of forskolin/ NECA-stimulated cAMP accumulation by measuring NECA-mediated cAMP stimulation at 1 uM incubated with NECA for 30 mins by LANCE cAMP detection assay	F	7.32	pEC50	47.86	nM	EC50	ACS Med Chem Lett (2022) 13: 923-934 [PMID:35707146]
A1 receptor/Adenosine A1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099]
ChEMBL	Displacement of [3H]-(R)-PIA from Adenosine A1 receptor of rat cerebral cortex membranes	B	6.52	pKi	305	nM	Ki	J Med Chem (1996) 39: 4142-4148 [PMID:8863790]
ChEMBL	Displacement of specific [3H](R)-PIA binding at adenosine A1 receptor from rat brain membranes.	B	6.52	pKi	305	nM	Ki	J Med Chem (1997) 40: 2596-2608 [PMID:9258367]
ChEMBL	Inhibition of [3H]DPCPX binding to rat Adenosine A1 receptor expressed in CHO cells	B	6.52	pKi	305	nM	Ki	J Med Chem (2002) 45: 3703-3708 [PMID:12166943]
ChEMBL	Displacement of [3H]CCPA from rat adenosine A1 receptor expressed in CHO cells after 60 mins by gamma counter	B	7.28	pKi	53	nM	Ki	J Med Chem (2012) 55: 5676-5703 [PMID:22468757]
ChEMBL	Binding affinity was determined in radioligand binding assay at rat brain adenosine A1 receptor vs [3H]R-PIA	B	7.28	pKi	52.7	nM	Ki	J Med Chem (1998) 41: 2835-2845 [PMID:9667972]
ChEMBL	Displacement of [3H]DPCPX from Adenosine A1 receptor of rat brain membranes	B	7.28	pKi	52.7	nM	Ki	J Med Chem (1999) 42: 4473-4478 [PMID:10579811]
A2A receptor/Adenosine A2a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274]
ChEMBL	Displacement of [3H]CGS21680 from human recombinant adenosine A2A receptor expressed in CHO cells after 60 mins by gamma counter	B	7.6	pKi	25	nM	Ki	J Med Chem (2012) 55: 5676-5703 [PMID:22468757]
A2A receptor/Adenosine A2a receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543]
ChEMBL	Displacement of [3H]-CGH 21680 from Adenosine A2A receptor of rat striatal membranes	B	7.28	pKi	52	nM	Ki	J Med Chem (1996) 39: 4142-4148 [PMID:8863790]
ChEMBL	Displacement of specific [3H]-CGS- 21680 binding at Adenosine A2A receptor in rat striatal membranes.	B	7.28	pKi	52	nM	Ki	J Med Chem (1997) 40: 2596-2608 [PMID:9258367]
ChEMBL	Inhibition of [3H]CGS-21680 binding to rat Adenosine A2A receptor expressed in HEK293 cells	B	7.28	pKi	52	nM	Ki	J Med Chem (2002) 45: 3703-3708 [PMID:12166943]
ChEMBL	Binding affinity was determined in radioligand binding assay at rat striatal Adenosine A2A receptor vs [3H]-CGS- 21680	B	7.99	pKi	10.3	nM	Ki	J Med Chem (1998) 41: 2835-2845 [PMID:9667972]
ChEMBL	Displacement of [3H]-SCH- 58261 from Adenosine A2A receptor in rat striatal membranes	B	7.99	pKi	10.3	nM	Ki	J Med Chem (1999) 42: 4473-4478 [PMID:10579811]
A3 receptor/Adenosine A3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8]
ChEMBL	Binding affinity to wild type A3R (unknown origin) expressed in Flp-In-CHO cells assessed as dissociation constant by by Schild analysis	B	10.01	pKd	0.1	nM	Kd	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Binding affinity to Nluc-A3R (unknown origin) expressed in HEK293 cells by nanoBRET assay	B	10.11	pKd	0.08	nM	Kd	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Displacement of [3H]NECA from human adenosine A3 receptor expressed in human HeLa cells incubated for 30 mins by fluorescence polarization binding assay	B	5.77	pKi	1700	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Displacement of CELT-228 from human adenosine A3 receptor expressed in human HeLa cells incubated for 30 mins by fluorescence polarization binding assay	B	5.85	pKi	1400	nM	Ki	ACS Med Chem Lett (2022) 13: 243-249 [PMID:35178181]
ChEMBL	Binding affinity to wild type A3R (unknown origin) expressed in CHO-K1 cells assessed as equilibrium binding affinity constant by nanoBRET assay	B	7.19	pKi	64.57	nM	Ki	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Antagonist activity at human adenosine A3 receptor expressed in Flp-In-CHO cell membrane assessed as inhibition of NECA-induced cAMP accumulation in presence of forskolin measured after 40 mins by alpha screen LANCE assay	F	8.3	pKi	5	nM	Ki	J Med Chem (2021) 64: 8161-8178 [PMID:34120444]
ChEMBL	Competitive binding affinity to human adenosine A3 receptor expressed in CHO cells after 90 mins by flow cytometric analysis in presence of MRS5449	B	8.46	pKi	3.46	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [3H]HMRS7799 from human adenosine A3 receptor expressed in HEK293 cells by radioligand binding assay	B	8.49	pKi	3.24	nM	Ki	ACS Med Chem Lett (2022) 13: 623-631 [PMID:35450351]
ChEMBL	Displacement of [125I]I-AB-MECA from human adenosine A3 receptor expressed in HEK293 cells by radioligand binding assay	B	8.53	pKi	2.97	nM	Ki	ACS Med Chem Lett (2022) 13: 623-631 [PMID:35450351]
ChEMBL	Displacement of [3H]NECA from human recombinant adenosine A3 receptor expressed in human HeLa cell membrane	B	8.61	pKi	2.48	nM	Ki	Bioorg Med Chem (2010) 18: 2081-2088 [PMID:20202853]
ChEMBL	Bnding affinity in membrane of CHO cell stably expressing recombinant human Adenosine A3 receptor using [3H]8-ethyl-4-methyl-2-phenyl-(8R)-4,5,7,8-tetrahydro-1H-imidazo[2,1-i]-purin-5-one as radioligand	B	8.92	pKi	1.2	nM	Ki	J Med Chem (2002) 45: 4471-4484 [PMID:12238926]
ChEMBL	Binding affinity in membrane of CHO cell stably expressing recombinant human Adenosine A3 receptor in the presence of GTPgammaS using [3H]8-ethyl-4-methyl-2-phenyl-(8R)-4,5,7,8-tetrahydro-1H-imidazo[2,1-i]-purin-5-one as radioligand	B	8.92	pKi	1.2	nM	Ki	J Med Chem (2002) 45: 4471-4484 [PMID:12238926]
ChEMBL	In vitro binding affinity at human Adenosine A3 receptor from HEK293 cells by [125I]AB-MECA displacement.	B	9.19	pKi	0.65	nM	Ki	J Med Chem (1996) 39: 4142-4148 [PMID:8863790]
ChEMBL	Displacement of [125I]I-AB-MECA from human adenosine A3 receptor expressed in CHO cells after 90 mins by gamma counting analysis	B	9.19	pKi	0.65	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [125]AB-MECA from human Adenosine A3 receptor expressed in HEK293 cells	B	9.19	pKi	0.65	nM	Ki	J Med Chem (1999) 42: 4473-4478 [PMID:10579811]
ChEMBL	Antagonist activity at human recombinant adenosine A3 receptor	B	9.19	pKi	0.65	nM	Ki	Bioorg Med Chem (2015) 23: 9-21 [PMID:25497490]
ChEMBL	In vitro binding affinity against human Adenosine A3 receptor	B	9.19	pKi	0.65	nM	Ki	Bioorg Med Chem Lett (2000) 10: 31-34 [PMID:10636237]
ChEMBL	Displacement of specific [125I]AB-MECA binding at human adenosine A3 receptor.	B	9.19	pKi	0.65	nM	Ki	J Med Chem (1997) 40: 2596-2608 [PMID:9258367]
ChEMBL	Inhibition of [125I]AB-MECA binding to human Adenosine A3 receptor expressed in HEK cells	B	9.19	pKi	0.65	nM	Ki	J Med Chem (2002) 45: 3703-3708 [PMID:12166943]
ChEMBL	Binding affinity at cloned human adenosine A3 receptor expressed in HEK293 cells was determined using [125I]AB-MECA as radioligand	B	9.19	pKi	0.65	nM	Ki	J Med Chem (1998) 41: 2835-2845 [PMID:9667972]
ChEMBL	Displacement of [3H]MRE 3008F20 from human adenosine A3 receptor expressed in CHO cells	B	9.19	pKi	0.65	nM	Ki	J Med Chem (2006) 49: 4085-4097 [PMID:16821770]
ChEMBL	Antagonist activity at human adenosine A3 receptor	F	9.19	pKi	0.65	nM	Ki	Bioorg Med Chem Lett (2011) 21: 2898-2905 [PMID:21511471]
GtoPdb	-	-	9.2	pKi	0.63	nM	Ki	Neuropharmacology (1997) 36: 1157-65 [PMID:9364471]; J Med Chem (1996) 39: 4142-8 [PMID:8863790]; Auton Autacoid Pharmacol (2006) 26: 191-200 [PMID:16553647]; Br J Pharmacol (2001) 132: 1017-26 [PMID:11226132]
ChEMBL	Displacement of [125I]I-AB-MECA from human recombinant adenosine A3 receptor expressed in CHO cells after 60 mins by gamma counter	B	9.23	pKi	0.59	nM	Ki	J Med Chem (2012) 55: 5676-5703 [PMID:22468757]
ChEMBL	Displacement of CA200645 from NLuc human A3 receptor expressed in HEK293 cells assessed as inhibition constant by NanoBRET binding assay	B	9.94	pKi	0.11	nM	Ki	ACS Med Chem Lett (2022) 13: 923-934 [PMID:35707146]
ChEMBL	Antagonist activity at human adenosine A3 receptor expressed in CHO cells by [S35]GTPgammaS binding assay	F	8.14	pIC50	7.2	nM	IC50	J Med Chem (2012) 55: 5676-5703 [PMID:22468757]
ChEMBL	Antagonist activity at wild type A3R (unknown origin) expressed in Flp-In-CHO cells assessed as inhibition of NECA reduced forskolin stimulated cAMP accumulation incubated for 30 mins	F	10.01	pIC50	0.1	nM	IC50	J Med Chem (2022) 65: 13305-13327 [PMID:36173355]
ChEMBL	Antagonist activity at human wild type A3R expressed in Flp-In-CHO cells assessed as inhibition of forskolin/ NECA-stimulated cAMP accumulation by measuring NECA-mediated cAMP stimulation at 10 uM incubated with NECA for 30 mins by LANCE cAMP detection assay	F	7.44	pEC50	36.31	nM	EC50	ACS Med Chem Lett (2022) 13: 923-934 [PMID:35707146]
A3 receptor/Adenosine A3 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3360] [GtoPdb: 21] [UniProtKB: P28647]
ChEMBL	Binding affinity of adenosine derivative for endogenous rat Adenosine A3 receptor expressed on CHO cell	B	8	pKi	>10	nM	Ki	J Med Chem (2002) 45: 4471-4484 [PMID:12238926]
5-HT1D receptor/Serotonin 1d (5-HT1d) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1983] [GtoPdb: 3] [UniProtKB: P28221]
ChEMBL	PDSP Secondary Binding target: HTR1D - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.	B	5	pKi	>10000	nM	Ki	EUbOPEN Chemogenomics Library - PDSP Secondary Binding
A2B receptor in Human [GtoPdb: 20] [UniProtKB: P29275]
GtoPdb	-	-	6.5	pKi	-	-	-	Biochem Pharmacol (2004) 68: 305-12 [PMID:15194002]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]