ChEMBL ligand: CHEMBL17127 (SCH-58261)
A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542] Adenosine A1 receptor in Bovine [ChEMBL: CHEMBL4975] [UniProtKB: P28190] A1 receptor/Adenosine A1 receptor in Rat [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099] There should be some charts here, you may need to enable JavaScript!
A2A receptor/Adenosine A2a receptor in Human [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274] A2A receptor/Adenosine A2a receptor in Rat [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543] There should be some charts here, you may need to enable JavaScript!
A2B receptor/Adenosine A2b receptor in Human [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275] A2B receptor/Adenosine A2b receptor in Rat [ChEMBL: CHEMBL2592] [GtoPdb: 20] [UniProtKB: P29276] There should be some charts here, you may need to enable JavaScript!
A3 receptor/Adenosine A3 receptor in Human [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A1 receptor expressed in HEK293 cells after 60 mins by rapid filtration assay	B	6.23	pKi	594.1	nM	Ki	Bioorg Med Chem (2010) 18: 2491-2500 [PMID:20303771]
ChEMBL	Inhibition of human recombinant adenosine A1 receptor	B	6.26	pKi	549	nM	Ki	J Med Chem (2014) 57: 3623-3650 [PMID:24164628]
ChEMBL	Displacement of [3H]CHA from human Adenosine A1 receptor expressed in CHO cells	B	6.26	pKi	549	nM	Ki	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
ChEMBL	Displacement of specific [3H]DPCPX binding at human Adenosine A1 receptor expressed in CHO cells.	B	6.26	pKi	549	nM	Ki	J Med Chem (2002) 45: 115-126 [PMID:11754583]
ChEMBL	Displacement of [3H]-DPCPX from human adenosine A1 receptor expressed in CHO cells after 120 mins by liquid scintillation counting	B	6.26	pKi	549	nM	Ki	Bioorg Med Chem (2012) 20: 1046-1059 [PMID:22204739]
GtoPdb	-	-	6.3	pKi	-	-	-	J Biol Chem (1994) 269: 2373-6 [PMID:8300561]; Naunyn Schmiedebergs Arch Pharmacol (1999) 359: 7-10 [PMID:9933143]; Nat Rev Drug Discov (2006) 5: 247-64 [PMID:16518376]
ChEMBL	Binding affinity against adenosine A1 receptor in human cortical membranes using [3H]CHA as radioligand	B	6.33	pKi	463	nM	Ki	J Med Chem (2004) 47: 3019-3031 [PMID:15163184]
ChEMBL	Binding affinity against human Adenosine A1 receptor	B	6.54	pKi	287	nM	Ki	Bioorg Med Chem Lett (2004) 14: 3307-3312 [PMID:15149696]
ChEMBL	Antagonist activity at adenosine A1 receptor (unknown origin)	F	6.92	pKi	121	nM	Ki	Eur J Med Chem (2022) 237: 114378-114378 [PMID:35462165]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A1 receptor membrane (unknown origin) measured after 60 mins by scintillation counting analysis	B	7.21	pKi	62	nM	Ki	J Med Chem (2021) 64: 16573-16597 [PMID:34783558]
Adenosine A1 receptor in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4975] [UniProtKB: P28190]
ChEMBL	Binding affinity against adenosine A1 receptor in bovine cortical membranes using [3H]CHA as radioligand	B	6.45	pKi	357	nM	Ki	J Med Chem (2004) 47: 3019-3031 [PMID:15163184]
A1 receptor/Adenosine A1 receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL318] [GtoPdb: 18] [UniProtKB: P25099]
ChEMBL	Displacement of [3h]DPCPX from adenosine A receptor in rat cerebral cortex membrane	B	6.41	pKi	390	nM	Ki	J Med Chem (2004) 47: 6218-6229 [PMID:15566292]
ChEMBL	Binding affinity towards Adenosine A1 receptor of rat cerebral cortex using [3H]-DPCPX as radioligand	B	6.41	pKi	390	nM	Ki	Bioorg Med Chem Lett (2005) 15: 511-515 [PMID:15664803]
ChEMBL	Binding affinity against Adenosine A1 receptor from rat cerebral cortex was determined using [3H]DPCPX as radioligand	B	6.41	pKi	390	nM	Ki	Bioorg Med Chem Lett (2004) 14: 4831-4834 [PMID:15341933]
ChEMBL	Binding affinity against Adenosine A1 receptor from rat cerebral cortex was determined using [3H]DPCPX as radioligand	B	6.41	pKi	390	nM	Ki	Bioorg Med Chem Lett (2004) 14: 4835-4838 [PMID:15341934]
ChEMBL	Inhibition of [3H]DPCPX binding to rat cerebral cortex adenosine A1 receptor	B	6.41	pKi	390	nM	Ki	J Med Chem (2004) 47: 4291-4299 [PMID:15294001]
ChEMBL	Inhibition of [3H]DPCPX binding to adenosine A1 receptor of rat cerebral cortex	B	6.41	pKi	390	nM	Ki	J Med Chem (2005) 48: 2009-2018 [PMID:15771443]
ChEMBL	Displacement of [3H]CHA from rat cortical membrane Adenosine A1 receptor	B	6.92	pKi	121	nM	Ki	J Med Chem (2003) 46: 1229-1241 [PMID:12646033]
ChEMBL	Displacement of [3H]CHA from rat cortical membrane Adenosine A1 receptor. Parenthesis indicate 95% confidence limit.	B	6.92	pKi	121	nM	Ki	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
ChEMBL	Binding ability of adenosine A1 receptor by using [3H]CHA as radioligand in rat whole brain homogenate	B	6.92	pKi	121	nM	Ki	Bioorg Med Chem Lett (1994) 4: 2539-2544
ChEMBL	Inhibition of [3H]-CHA binding to Adenosine A1 receptor in rat whole brain homogenates.	B	6.92	pKi	121	nM	Ki	J Med Chem (1996) 39: 1164-1171 [PMID:8676354]
A2A receptor/Adenosine A2a receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL251] [GtoPdb: 19] [UniProtKB: P29274]
ChEMBL	Purified A2A protein was immobilized via His-tag on NTA chip. Small molecule ligands were analyzed in multiple cycle kinetoc mode. No regeneration was applied.	B	7.77	pKd	16.8	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	The A2A-StaR2 receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore. A minimum of three measurements were recorded for each interaction.	B	8.19	pKd	6.52	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	The A2A-StaR2 receptor has been purified from membranes in n-decyl-beta-D-maltoside as detergent. Then it has been reconstituted into rHDLs membrane discs.Ligands were prepared over a five-fold concentration series and injected in either a multi- or a single-cycle format depending on the kinetic parameters of the ligand. The actual concentrations as well as contact and dissociation times varied between the ligands depended on the kinetics and affinity of the interaction. The data were fitted to a 1:1 interaction model using the kinetics evaluation software from Biacore. A minimum of three measurements were recorded for each interaction.	B	8.4	pKd	4.02	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	A2A protein embedded into nanodisc was immobilized via His-tag on NTA chip. Small molecule ligands were analyzed in multiple cycle kinetic mode. No regeneration was applied.	B	8.48	pKd	3.34	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	A2A protein embedded into nanodisc was immobilized via 1D4 antibody on the CM5 sensors. Small molecule ligands were analyzed in multiple cycle kinetic mode. No regeneration was applied.	B	8.7	pKd	1.97	nM	Kd	K4DD drug target binding kinetics data
ChEMBL	Inhibition constant against human Adenosine A2a receptor using [3H]-SCH- 58261 as radioligand expressed in HEK cell membranes	B	8.37	pKi	4.3	nM	Ki	Bioorg Med Chem Lett (2005) 15: 2119-2122 [PMID:15808481]
ChEMBL	Binding affinity against adenosine A2 receptor in human striatal membranes using [3H]CGS-21680 as radioligand	B	8.49	pKi	3.25	nM	Ki	J Med Chem (2004) 47: 3019-3031 [PMID:15163184]
ChEMBL	Displacement of [3H]SCH58261 from adenosine A2A receptor membrane (unknown origin) measured after 60 mins by scintillation counting analysis	B	8.62	pKi	2.4	nM	Ki	J Med Chem (2021) 64: 16573-16597 [PMID:34783558]
ChEMBL	Binding affinity towards adenosine A2A receptor	B	8.64	pKi	2.3	nM	Ki	Bioorg Med Chem Lett (2005) 15: 4809-4813 [PMID:16153830]
ChEMBL	Antagonist activity at adenosine A2A receptor (unknown origin)	F	8.64	pKi	2.3	nM	Ki	Eur J Med Chem (2022) 237: 114378-114378 [PMID:35462165]
ChEMBL	Binding affinity against adenosine A2 receptor in bovine striatal membranes using [3H]-CGS-21,680 as radioligand	B	8.7	pKi	2	nM	Ki	J Med Chem (2004) 47: 3019-3031 [PMID:15163184]
ChEMBL	Antagonist activity at human adenosine A2A receptor	F	8.7	pKi	2	nM	Ki	Bioorg Med Chem Lett (2008) 18: 4204-4209 [PMID:18558486]
ChEMBL	Antagonist activity at human adenosine A2A receptor	F	8.7	pKi	2	nM	Ki	Bioorg Med Chem Lett (2008) 18: 4199-4203 [PMID:18562199]
ChEMBL	Competitive binding affinity to human adenosine A2A receptor expressed in HEK293 cells after 60 mins by fluorescence polarization assay in presence of MRS5346	B	8.72	pKi	1.9	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Displacement of [3H]ZM241385 from human adenosine A2A receptor expressed in HEK293 cells after 60 mins by rapid filtration assay	B	8.91	pKi	1.23	nM	Ki	Bioorg Med Chem (2010) 18: 2491-2500 [PMID:20303771]
ChEMBL	Inhibition of human recombinant adenosine receptor A2a	F	8.96	pKi	1.1	nM	Ki	J Med Chem (2014) 57: 3623-3650 [PMID:24164628]
ChEMBL	Displacement of [3H]-SCH- 58261 from human Adenosine A2A receptor expressed in CHO cells	B	8.96	pKi	1.1	nM	Ki	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
ChEMBL	Displacement of specific [3H]-SCH- 58261 binding at human Adenosine A2A receptor expressed in HEK293 cells.	B	8.96	pKi	1.1	nM	Ki	J Med Chem (2002) 45: 115-126 [PMID:11754583]
ChEMBL	Displacement of [3H]NECA from human adenosine A2A receptor expressed in CHO cells	B	8.96	pKi	1.1	nM	Ki	Bioorg Med Chem (2009) 17: 5259-5274 [PMID:19501513]
ChEMBL	Displacement of [3H]-ZM 241385 from human adenosine A2A receptor expressed in CHO cells after 60 mins by liquid scintillation counting	B	8.96	pKi	1.1	nM	Ki	Bioorg Med Chem (2012) 20: 1046-1059 [PMID:22204739]
ChEMBL	Antagonist activity at human adenosine 2A receptor	B	8.96	pKi	1.1	nM	Ki	J Med Chem (2020) 63: 12196-12212 [PMID:32667814]
GtoPdb	-	-	9.2	pKi	-	-	-	Naunyn Schmiedebergs Arch Pharmacol (1999) 359: 7-10 [PMID:9933143]; Biochem Pharmacol (1999) 57: 65-75 [PMID:9920286]; Br J Pharmacol (1997) 121: 353-60 [PMID:9179373]
ChEMBL	Binding affinity to human adenosine A2A receptor	B	9.22	pKi	0.6	nM	Ki	J Med Chem (2008) 51: 4449-4455 [PMID:18637670]
ChEMBL	Binding affinity against human Adenosine A2a receptor (hA2a)	B	9.22	pKi	0.6	nM	Ki	Bioorg Med Chem Lett (2004) 14: 3307-3312 [PMID:15149696]
ChEMBL	Displacement of [3H]CGS21680 from human adenosine A2A receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting analysis	B	9.22	pKi	0.6	nM	Ki	Bioorg Med Chem Lett (2013) 23: 26-36 [PMID:23200243]
ChEMBL	Inhibition of A2aR (unknown origin) expressed in HEK293 cells incubated overnight by dual luciferase reporter assay	B	7.4	pIC50	40	nM	IC50	US-20200345725-A1. Methods and Compositions for Treating Cancer (2020)
ChEMBL	Inhibition of 1 uM NECA-stimulated cyclic AMP levels in human platelets	F	7.92	pIC50	12	nM	IC50	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
A2A receptor/Adenosine A2a receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL302] [GtoPdb: 19] [UniProtKB: P30543]
ChEMBL	Binding affinity to adenosine receptor A2a in rat striatum	B	9.15	pKd	0.7	nM	Kd	J Med Chem (2014) 57: 3623-3650 [PMID:24164628]
ChEMBL	Displacement [3H]-CGS-21,680 from Adenosine A2A receptor in rat striatal membrane.	B	5.72	pKi	1900	nM	Ki	J Med Chem (1996) 39: 398-406 [PMID:8558508]
ChEMBL	Displacement of [3H]ZM241385 from adenosine A2A receptor in rat brain membrane	B	7.43	pKi	37	nM	Ki	J Med Chem (2004) 47: 6218-6229 [PMID:15566292]
ChEMBL	Binding affinity towards Adenosine A2A receptor of rat brain tissues using [3H]ZM-241385 as radioligand	B	7.43	pKi	37	nM	Ki	Bioorg Med Chem Lett (2005) 15: 511-515 [PMID:15664803]
ChEMBL	Binding affinity against Adenosine A2a receptor from rat brain tissue was determined using [3H]ZM-241385 as radioligand	B	7.43	pKi	37	nM	Ki	Bioorg Med Chem Lett (2004) 14: 4831-4834 [PMID:15341933]
ChEMBL	Binding affinity against Adenosine A2a receptor determined using [3H]ZM-241385 as radioligand	B	7.43	pKi	37	nM	Ki	Bioorg Med Chem Lett (2004) 14: 4835-4838 [PMID:15341934]
ChEMBL	Inhibition of [3H]ZM-241,385 binding to rat brain adenosine A2a receptor	B	7.43	pKi	37	nM	Ki	J Med Chem (2004) 47: 4291-4299 [PMID:15294001]
ChEMBL	Inhibition of [3H]ZM-241385 binding to adenosine A2a receptor of rat brain tissue	B	7.43	pKi	37	nM	Ki	J Med Chem (2005) 48: 2009-2018 [PMID:15771443]
ChEMBL	Displacement of [3H]-CGS- 21680 from rat striatal membrane Adenosine A2A receptor	B	8.64	pKi	2.3	nM	Ki	J Med Chem (2003) 46: 1229-1241 [PMID:12646033]
ChEMBL	Displacement of [3H]-CGS- 21680 from rat striatal membranes Adenosine A2A receptor. Parenthesis indicate 95% confidence limits.	B	8.64	pKi	2.3	nM	Ki	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
ChEMBL	Binding ability of adenosine A2a receptor by using [3H]-CGS- as radioligand in rat striatal homogenate	B	8.64	pKi	2.3	nM	Ki	Bioorg Med Chem Lett (1994) 4: 2539-2544
ChEMBL	Inhibition of [3H]-CGS- 21680 binding to Adenosine A2A receptor in rat striatal homogenates.	B	8.64	pKi	2.3	nM	Ki	J Med Chem (1996) 39: 1164-1171 [PMID:8676354]
A2B receptor/Adenosine A2b receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL255] [GtoPdb: 20] [UniProtKB: P29275]
ChEMBL	Displacement of specific [3H]DPCPX binding at human adenosine A2B receptor expressed in HEK293 cells.	B	5	pKi	>10000	nM	Ki	J Med Chem (2002) 45: 115-126 [PMID:11754583]
ChEMBL	Displacement of [3H]-DPCPX from human adenosine A2B receptor expressed in HEK293 cells after 60 mins	B	5	pKi	>10000	nM	Ki	Bioorg Med Chem (2012) 20: 1046-1059 [PMID:22204739]
ChEMBL	Inhibition of human recombinant adenosine receptor A2b	B	5.95	pKi	1110	nM	Ki	J Med Chem (2014) 57: 3623-3650 [PMID:24164628]
ChEMBL	Inhibition of human adenosine 2B receptor	B	5.95	pKi	1110	nM	Ki	J Med Chem (2020) 63: 12196-12212 [PMID:32667814]
GtoPdb	-	-	6.5	pKi	-	-	-	Mol Pharmacol (2000) 58: 1349-56 [PMID:11093773]; Nat Rev Drug Discov (2006) 5: 247-64 [PMID:16518376]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A2B receptor membrane (unknown origin) measured after 60 mins by scintillation counting analysis	B	6.59	pKi	255.7	nM	Ki	J Med Chem (2021) 64: 16573-16597 [PMID:34783558]
ChEMBL	Inhibition of A2bR (unknown origin) expressed in HEK293 cells incubated overnight by dual luciferase reporter assay	B	5.7	pIC50	>2000	nM	IC50	US-20200345725-A1. Methods and Compositions for Treating Cancer (2020)
A2B receptor/Adenosine A2b receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2592] [GtoPdb: 20] [UniProtKB: P29276]
ChEMBL	Displacement of [3H]-CGS- 21680 from rat striatal membrane adenosine A2B receptor	B	6	pKi	>1000	nM	Ki	J Med Chem (2003) 46: 1229-1241 [PMID:12646033]
A3 receptor/Adenosine A3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL256] [GtoPdb: 21] [UniProtKB: P0DMS8]
ChEMBL	Displacement of [3H]NECA from human adenosine A3 receptor expressed in CHO cells	B	5	pKi	>10000	nM	Ki	Bioorg Med Chem (2009) 17: 5259-5274 [PMID:19501513]
ChEMBL	Displacement of [125I]AB-MECA from human Adenosine A3 receptor expressed in HEK293 cells	B	5	pKi	>10000	nM	Ki	J Med Chem (1998) 41: 2126-2133 [PMID:9622554]
ChEMBL	Displacement of specific [3H]MRE3008-F20 binding at human adenosine A3 receptor expressed in CHO cells.	B	5	pKi	>10000	nM	Ki	J Med Chem (2002) 45: 115-126 [PMID:11754583]
ChEMBL	Displacement of [3H]-MRE-3008-F20 from human adenosine A3 receptor expressed in CHO cells after 120 mins by liquid scintillation counting	B	5	pKi	>10000	nM	Ki	Bioorg Med Chem (2012) 20: 1046-1059 [PMID:22204739]
ChEMBL	Displacement of [3H]DPCPX from human adenosine A3 receptor membrane (unknown origin) measured after 60 mins by scintillation counting analysis	B	5	pKi	>10000	nM	Ki	J Med Chem (2021) 64: 16573-16597 [PMID:34783558]
GtoPdb	-	-	5.92	pKi	1200	nM	Ki	Nat Rev Drug Discov (2006) 5: 247-64 [PMID:16518376]
ChEMBL	Inhibition of human recombinant adenosine A3 receptor	B	5.92	pKi	1200	nM	Ki	J Med Chem (2014) 57: 3623-3650 [PMID:24164628]
ChEMBL	Displacement of [3H]-AB MECA from human Adenosine A3 receptor expressed in HEK293 cells	B	6	pKi	>1000	nM	Ki	J Med Chem (2003) 46: 1229-1241 [PMID:12646033]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]