ChEMBL ligand: CHEMBL4746525
SET and MYND domain containing 3/Histone-lysine N-methyltransferase SMYD3 in Human [ChEMBL: CHEMBL2321643] [GtoPdb: 3313] [UniProtKB: Q9H7B4] There should be some charts here, you may need to enable JavaScript!
FYN proto-oncogene, Src family tyrosine kinase/Tyrosine-protein kinase Fyn in Human [ChEMBL: CHEMBL1841] [GtoPdb: 2026] [UniProtKB: P06241] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
SET and MYND domain containing 3/Histone-lysine N-methyltransferase SMYD3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2321643] [GtoPdb: 3313] [UniProtKB: Q9H7B4]
ChEMBL	Selectivity interaction (ITC) EUB0000221b SMYD3	B	6.88	pKd	133	nM	Kd	Selectivity Literature for EUbOPEN Chemogenomics Library wave 3
ChEMBL	Binding affinity to recombinant human SMYD3 expressed in Escherichia coli assessed as dissociation constant by isothermal titration calorimetry	B	6.88	pKd	133	nM	Kd	SLAS Discov (2021) 26: 947-960 [PMID:34154424]
ChEMBL	Selectivity interaction (SPR measurement) EUB0000221b SMYD3	B	7.01	pKd	97	nM	Kd	Selectivity Literature for EUbOPEN Chemogenomics Library wave 3
ChEMBL	Binding affinity to biotinylated SMYD3 (unknown origin) assessed as dissociation constant using S-adenosyl-l-methionine as substrate by surface plasmon resonance analysis	B	7.01	pKd	97	nM	Kd	SLAS Discov (2021) 26: 947-960 [PMID:34154424]
ChEMBL	Affinity Phenotypic Cellular interaction (Immunoblot (inhibition of MAP3K2 methylation in HeLa cells)) EUB0000221b SMYD3	B	6.74	pIC50	183	nM	IC50	Affinity Phenotypic Cellular Literature for EUbOPEN Chemogenomics Library wave 3
ChEMBL	Inhibition of SMYD3 catalytic inactive mutant co-expressed with HA-tagged MAP3K2 transfected in human HeLa cells assessed as reduction of MAP3K2-K260me3 level incubated for 20 hrs by Western blotting analysis	B	6.74	pIC50	183	nM	IC50	SLAS Discov (2021) 26: 947-960 [PMID:34154424]
ChEMBL	Affinity Biochemical interaction (Scintillation proximity assay (SPA)) EUB0000221b SMYD3	B	7.06	pIC50	88	nM	IC50	Affinity Biochemical Literature for EUbOPEN Chemogenomics Library wave 3
ChEMBL	Binding affinity to recombinant human SMYD3 expressed in Escherichia coli assessed as catalytic inhibition using DYDNPIFEKFGKGGTYPRRYHVSYHGK-Biotin as substrate by scintillation proximity assay	B	7.06	pIC50	88	nM	IC50	SLAS Discov (2021) 26: 947-960 [PMID:34154424]
GtoPdb	Measuring inhibition of methylation of MEKK2 peptide in vitro	-	7.06	pIC50	88	nM	IC50	BAY-6035 A potent, peptide-competitive chemical probe for SMYD3. thesgc.org
FYN proto-oncogene, Src family tyrosine kinase/Tyrosine-protein kinase Fyn in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1841] [GtoPdb: 2026] [UniProtKB: P06241]
ChEMBL	Selectivity interaction (Kinase panel (HTRF assay)) EUB0000221b FYN	B	5.24	pIC50	5710	nM	IC50	Selectivity Literature for EUbOPEN Chemogenomics Library wave 3

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]