ChEMBL ligand: CHEMBL4552020
spindlin 1/Spindlin-1 in Human [ChEMBL: CHEMBL4523509] [GtoPdb: 3274] [UniProtKB: Q9Y657] There should be some charts here, you may need to enable JavaScript!
Spindlin-2B in Human [ChEMBL: CHEMBL4523440] [UniProtKB: Q9BPZ2] There should be some charts here, you may need to enable JavaScript!
Spindlin-3 in Human [ChEMBL: CHEMBL4523325] [UniProtKB: Q5JUX0] There should be some charts here, you may need to enable JavaScript!
Spindlin-4 in Human [ChEMBL: CHEMBL4523321] [UniProtKB: Q56A73] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
spindlin 1/Spindlin-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523509] [GtoPdb: 3274] [UniProtKB: Q9Y657]
GtoPdb	Binding affinity determined by ITC assay	-	7.04	pKd	91	nM	Kd
ChEMBL	Inhibition of recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by isothermal titration calorimetry	B	7.04	pKd	91	nM	Kd	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Inhibition of C-terminal Halo Tag-histone H3.3/N-terminal Nano Luciferase-fused full length SPIN1 (unknown origin) transfected in human U2OS cells assessed as reduction in SPIN1 and histone H3 interaction incubated for 24 hrs by NanoBRET assay	B	5.49	pIC50	3200	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Inhibition of FL-H3K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 30 mins by fluorescence polarization displacement assay	B	6.61	pIC50	243	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
GtoPdb	Inhibitor potency determined by AlphaLISA	-	7.11	pIC50	77	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
ChEMBL	Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay	B	7.11	pIC50	77	nM	IC50	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
Spindlin-2B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523440] [UniProtKB: Q9BPZ2]
ChEMBL	Inhibition of recombinant human N-terminal His6-tagged SPIN2B (45 to 258 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by isothermal titration calorimetry	B	6.77	pKd	170	nM	Kd	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
Spindlin-3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523325] [UniProtKB: Q5JUX0]
ChEMBL	Inhibition of recombinant human N-terminal His6-tagged SPIN3 (27 to 258 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by isothermal titration calorimetry	B	6.77	pKd	170	nM	Kd	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]
Spindlin-4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523321] [UniProtKB: Q56A73]
ChEMBL	Inhibition of recombinant human N-terminal His6-tagged SPIN4 (36 to 249 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by isothermal titration calorimetry	B	6.77	pKd	170	nM	Kd	J Med Chem (2019) 62: 8996-9007 [PMID:31260300]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]