calpeptin [Ligand Id: 11643] activity data from GtoPdb and ChEMBL

Click here for a description of the charts and data table

Please tell us if you are using this feature and what you think!

ChEMBL ligand: CHEMBL92708 (Calpeptin)
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
There should be some charts here, you may need to enable JavaScript!
  • CoV Replicase polyprotein 1ab/CoV RNA-dependent RNA polymerase/CoV Non-structural protein 15/CoV Non-structural protein 13/Replicase polyprotein 1ab in Severe acute respiratory syndrome coronavirus 2 [ChEMBL: CHEMBL4523582] [GtoPdb: 3125, 3139, 3206, 3261] [UniProtKB: P0DTD1]
There should be some charts here, you may need to enable JavaScript!
  • CoV 3C-like (main) protease in SARS-CoV-2 [GtoPdb: 3111]
There should be some charts here, you may need to enable JavaScript!
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
calpain 1/Calpain 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3891] [GtoPdb: 2336] [UniProtKB: P07384]
ChEMBL The binding affinity against calpain. B 8.15 pKi 7 nM Ki J Med Chem (2000) 43: 305-341 [PMID:10669559]
ChEMBL Inhibitory activity against calpain. B 6 pIC50 1000 nM IC50 J Med Chem (2000) 43: 305-341 [PMID:10669559]
ChEMBL Inhibition of human erythrocytes mu-calpain using Pep1 as substrate incubated for 30 mins under shaking condition in presence of CaCl2 by fluorescence assay B 6.53 pIC50 297 nM IC50 Eur J Med Chem (2016) 121: 433-444 [PMID:27318120]
ChEMBL Inhibition of calpain (unknown origin) using Suc-LY-AMC as fluorogenic substrate after 60 mins by spectrofluorometric analysis B 7.28 pIC50 52 nM IC50 Eur J Med Chem (2018) 157: 946-959 [PMID:30165342]
ChEMBL Inhibitory activity against Calpain 1 in platelets. B 7.4 pIC50 40 nM IC50 J Med Chem (2000) 43: 305-341 [PMID:10669559]
GtoPdb Inhibition of recombinant human calpain 1 - 8.3 pIC50 5 nM IC50 Bioorg Med Chem Lett (1997) 7: 539-544
ChEMBL Inhibitory activity evaluated against recombinant human calpain 1 B 8.3 pIC50 5 nM IC50 Bioorg Med Chem Lett (1997) 7: 539-544
Calpain-9 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523126] [UniProtKB: O14815]
ChEMBL Inhibition of human recombinant mini-CAPN9 pre-incubated for 10 mins before calpain FRET peptide 5-FAM /QXL520 substrate addition by FLIPR assay B 5.55 pIC50 2840 nM IC50 US-20180318405-A1. Targeting capn9/capns2 activity as a therapeutic strategy for the treatment of myofibroblast differentiation and associated pathologies (null)
cathepsin B/Cathepsin B in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4072] [GtoPdb: 2343] [UniProtKB: P07858]
ChEMBL Inhibition of cathepsin B (unknown origin) using RR-AMC as substrate preincubated for 30 mins followed by substrate addition and further incubated for 30 mins under shaking condition by fluorescence assay B 4 pIC50 >100000 nM IC50 Eur J Med Chem (2016) 121: 433-444 [PMID:27318120]
cathepsin K/Cathepsin K in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL268] [GtoPdb: 2350] [UniProtKB: P43235]
ChEMBL Inhibitory activity against recombinant human cathepsin K B 9.96 pIC50 0.11 nM IC50 Bioorg Med Chem Lett (2004) 14: 275-278 [PMID:14684342]
ChEMBL Inhibitory activity against human cathepsin K B 9.96 pIC50 0.11 nM IC50 Bioorg Med Chem Lett (2004) 14: 719-722 [PMID:14741275]
GtoPdb - - 9.96 pIC50 0.11 nM IC50 Bioorg Med Chem Lett (2004) 14: 719-22 [PMID:14741275]
cathepsin L/Cathepsin L in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3837] [GtoPdb: 2351] [UniProtKB: P07711]
ChEMBL Inhibition of cathepsin L (unknown origin) using Z-FR-AMC as substrate preincubated for 30 mins followed by substrate addition and further incubated for 30 mins under shaking condition by fluorescence assay B 7.14 pIC50 72 nM IC50 Eur J Med Chem (2016) 121: 433-444 [PMID:27318120]
CoV Replicase polyprotein 1ab/CoV RNA-dependent RNA polymerase/CoV Non-structural protein 15/CoV Non-structural protein 13/Replicase polyprotein 1ab in Severe acute respiratory syndrome coronavirus 2 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523582] [GtoPdb: 3125, 3139, 3206, 3261] [UniProtKB: P0DTD1]
ChEMBL Inhibition of SARS-CoV-2 Main protease expressed in Escherichia coli BL21 (DE3) preincubated for 30 mins followed by addition of Dabcyl-KTSAVLQ/SGFRKME(Edans) substrate and measured after 1 hr by FRET assay B 4.97 pIC50 10690 nM IC50 Bioorg Med Chem Lett (2021) 48: 128263-128263 [PMID:34271072]
ChEMBL Inhibition of SARS-CoV-2 3CLpro preincubated for 30 mins followed by addition of (Dabcyl)KTSAVLQSGFRKM(Glu) peptide substrate and measured after 1.5 hrs by FRET assay B 5 pIC50 >10000 nM IC50 Bioorg Med Chem Lett (2021) 48: 128263-128263 [PMID:34271072]
ChEMBL Inhibition of SARS-CoV-2 3CL (3264 to 3569 residues) preincubated for 30 mins followed by addition of Dabcyl-KTSAVLQSGFRKME(Edans) substrate and measured after 1 hr by flourescence based plate reader assay B 5.32 pIC50 4810 nM IC50 Bioorg Med Chem Lett (2021) 48: 128263-128263 [PMID:34271072]
CoV 3C-like (main) protease in SARS-CoV-2 [GtoPdb: 3111]
GtoPdb - - 4.97 pIC50 10690 nM IC50 Cell Res (2020) 30: 678-692 [PMID:32541865]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]