ChEMBL ligand: CHEMBL3675743
GPR4/G-protein coupled receptor 4 in Human [ChEMBL: CHEMBL3638324] [GtoPdb: 84] [UniProtKB: P46093] GPR4/G-protein coupled receptor 4 in Mouse [ChEMBL: CHEMBL4105764] [GtoPdb: 84] [UniProtKB: Q8BUD0] GPR4/G-protein coupled receptor 4 in Rat [ChEMBL: CHEMBL4105950] [GtoPdb: 84] [UniProtKB: Q4KLH9] There should be some charts here, you may need to enable JavaScript!
Kv11.1/HERG in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!
H3 receptor/Histamine H3 receptor in Human [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1] There should be some charts here, you may need to enable JavaScript!
μ receptor/Mu opioid receptor in Human [ChEMBL: CHEMBL233] [GtoPdb: 319] [UniProtKB: P35372] There should be some charts here, you may need to enable JavaScript!
GPR68/Ovarian cancer G-protein coupled receptor 1 in Human [ChEMBL: CHEMBL3713916] [GtoPdb: 114] [UniProtKB: Q15743] There should be some charts here, you may need to enable JavaScript!
GPR65/Psychosine receptor in Human [ChEMBL: CHEMBL3714081] [GtoPdb: 113] [UniProtKB: Q8IYL9] There should be some charts here, you may need to enable JavaScript!
5-HT3A/Serotonin 3a (5-HT3a) receptor in Human [ChEMBL: CHEMBL1899] [GtoPdb: 373] [UniProtKB: P46098] There should be some charts here, you may need to enable JavaScript!
σ2/Sigma intracellular receptor 2 in Human [ChEMBL: CHEMBL4105907] [GtoPdb: 2553] [UniProtKB: Q5BJF2] There should be some charts here, you may need to enable JavaScript!
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
GPR4/G-protein coupled receptor 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3638324] [GtoPdb: 84] [UniProtKB: P46093]
ChEMBL	Radio Ligand Binding Assay: Serial dilutions of compounds (stock in 10 mM DMSO) are prepared by first diluting the compounds in DMSO followed by a 1:50 dilution into assay buffer (10 mM HEPES, pH 8.0, 100 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.5% fatty acid-free BSA, 0.05% Tween-20). The radioligand [3H]4 (example 39) (specific activity 1500 GBq/mmol) is diluted directly into the assay buffer immediately before use to obtain a 20 nM solution. The desired amount of membranes (20 μg/well) is diluted with assay buffer. 50 μL of pre-diluted compound and 50 μL of [3H]4 (example 39) is placed into the bottom of a 96-well well plate. 100 μL of the membrane-suspension is added and the plate stirred for 60 minutes. The reaction is stopped by transfer onto the filter of a 96-well GF/C filter plate (soaked for 1 hour in 0.25% PEI) using a cell harvester.	B	7.13	pIC50	74	nM	IC50	US-8748435-B2. Pyrazolo pyrimidine derivatives (2014)
GtoPdb	Measuring antagonism of pH-dependent cAMP release in HELA cells expressing human GPR4.	-	7.15	pIC50	70	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
ChEMBL	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay	F	7.15	pIC50	70	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
ChEMBL	Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins in presence of 40% rat plasma by HTRF assay	F	7.17	pIC50	67	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
GPR4/G-protein coupled receptor 4 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105764] [GtoPdb: 84] [UniProtKB: Q8BUD0]
ChEMBL	Antagonist activity at mouse GPR4 expressed in HEK cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay	F	6.28	pIC50	530	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
GPR4/G-protein coupled receptor 4 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105950] [GtoPdb: 84] [UniProtKB: Q4KLH9]
ChEMBL	Antagonist activity at rat GPR4 expressed in HEK cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay	F	5.74	pIC50	1840	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
GtoPdb	Measuring antagonism of pH-dependent cAMP release in HEK cells expressing rat GPR4.	-	5.74	pIC50	1840	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
Kv11.1/HERG in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Displacement of [3H]dofetilide from recombinant human ERG expressed in HEK293 cell membranes after 90 mins by liquid scintillation counting method	B	4.72	pIC50	19000	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
H3 receptor/Histamine H3 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL264] [GtoPdb: 264] [UniProtKB: Q9Y5N1]
ChEMBL	Displacement of [3H]-R(-)-alpha-Methyl[imidazole-2.5(n)]histamine from human recombinant histamine H3 receptor expressed in CHOK1 cell membranes after 1 hr by scintillation counting method	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
μ receptor/Mu opioid receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL233] [GtoPdb: 319] [UniProtKB: P35372]
ChEMBL	PDSP Secondary Binding target: OPRM1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.	B	5.11	pKi	7723.25	nM	Ki	EUbOPEN Chemogenomics Library - PDSP Secondary Binding
GPR68/Ovarian cancer G-protein coupled receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3713916] [GtoPdb: 114] [UniProtKB: Q15743]
ChEMBL	Inhibition of OGR1 (unknown origin) expressed in human HeLa cells assessed as reduction in cAMP accumulation	F	4.7	pIC50	>20000	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
GPR65/Psychosine receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3714081] [GtoPdb: 113] [UniProtKB: Q8IYL9]
ChEMBL	Inhibition of TDAG8 (unknown origin) expressed in human HeLa cells assessed as reduction in cAMP accumulation	F	4.7	pIC50	>20000	nM	IC50	J Med Chem (2017) 60: 3672-3683 [PMID:28445047]
5-HT3A/Serotonin 3a (5-HT3a) receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1899] [GtoPdb: 373] [UniProtKB: P46098]
ChEMBL	PDSP Secondary Binding target: HTR3A - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.	B	6.44	pKi	360.29	nM	Ki	EUbOPEN Chemogenomics Library - PDSP Secondary Binding
σ2/Sigma intracellular receptor 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4105907] [GtoPdb: 2553] [UniProtKB: Q5BJF2]
ChEMBL	PDSP Secondary Binding target: TMEM97 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.	B	6.31	pKi	488.65	nM	Ki	EUbOPEN Chemogenomics Library - PDSP Secondary Binding
sigma non-opioid intracellular receptor 1/Sigma opioid receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL287] [GtoPdb: 2552] [UniProtKB: Q99720]
ChEMBL	PDSP Secondary Binding target: SIGMAR1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.	B	5.7	pKi	1999.86	nM	Ki	EUbOPEN Chemogenomics Library - PDSP Secondary Binding

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]