ChEMBL ligand: CHEMBL106820
NG,NG-Dimethylarginine dimethylaminohydrolase 1/N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 in Human [ChEMBL: CHEMBL6036] [GtoPdb: 1247] [UniProtKB: O94760] There should be some charts here, you may need to enable JavaScript!
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
NG,NG-Dimethylarginine dimethylaminohydrolase 1/N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6036] [GtoPdb: 1247] [UniProtKB: O94760]
ChEMBL	Inhibition of human DDAH1 by plate-reader assay	B	5.7	pKi	2000	nM	Ki	Bioorg Med Chem (2008) 16: 10205-10209 [PMID:19013076]
ChEMBL	Inhibition of 6xHis-tagged recombinant human DDAH1 expressed in Escherichia coli BL21 cells using NMMA as substrate measured after 30 mins by colder reagent based microplate reader assay	B	5.7	pKi	2000	nM	Ki	J Med Chem (2020) 63: 425-432 [PMID:31841335]
ChEMBL	Inhibition of human DDAH1 expressed in Escherichia coli BL21 cells assessed as inhibition constant measured after 30 mins by calorimetric assay	B	5.7	pKi	2000	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of human DDAH1 expressed using SMTC as substrate assessed as inhibition constant measured after 5 mins	B	5.7	pKi	2000	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
GtoPdb	-	-	5.7	pKi	2000	nM	Ki	Bioorg Med Chem (2008) 16: 10205-9 [PMID:19013076]
ChEMBL	Inhibition of human DDAH1 by plate-reader assay	B	4.89	pIC50	13000	nM	IC50	Bioorg Med Chem (2008) 16: 10205-10209 [PMID:19013076]
ChEMBL	Inhibition of human DDAH1 expressed in Escherichia coli BL21 cells measured after 30 mins by calorimetric assay	B	4.92	pIC50	12000	nM	IC50	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Inhibition of nNOS assessed as conversion of L-[3H]arginine to L-[3H]citrulline	B	7	pKi	100	nM	Ki	Bioorg Med Chem (2008) 16: 6193-6206 [PMID:18477512]
ChEMBL	Inhibition of human nNOS assessed as inhibition constant measured after 5 mins	B	7.05	pKi	90	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of rat nNOS transfected in HEK293 cells assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion by liquid scintillation counting analysis	B	7	pKi	100	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Inhibition of mouse iNOS expressed in Escherichia coli assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion by liquid scintillation counting analysis	B	4.22	pKi	60000	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]