ChEMBL ligand: CHEMBL2012938 (Vialinin A)
SUMO specific peptidase 1/Sentrin-specific protease 1 in Human [ChEMBL: CHEMBL1909484] [GtoPdb: 2414] [UniProtKB: Q9P0U3] There should be some charts here, you may need to enable JavaScript!
Ubiquitin carboxyl-terminal hydrolase 4 in Human [ChEMBL: CHEMBL2406900] [UniProtKB: Q13107] There should be some charts here, you may need to enable JavaScript!
ubiquitin specific peptidase 5/Ubiquitin carboxyl-terminal hydrolase 5 in Human [ChEMBL: CHEMBL6158] [GtoPdb: 2431] [UniProtKB: P45974] There should be some charts here, you may need to enable JavaScript!
ubiquitin C-terminal hydrolase L1/Ubiquitin carboxyl-terminal hydrolase isozyme L1 in Human [ChEMBL: CHEMBL6159] [GtoPdb: 2426] [UniProtKB: P09936] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
SUMO specific peptidase 1/Sentrin-specific protease 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1909484] [GtoPdb: 2414] [UniProtKB: Q9P0U3]
ChEMBL	Binding affinity to full-length recombinant human 6His-tagged SENP1 expressed in Escherichia coli BL21 (DE3)	B	5.97	pKi	1070	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4237-4240 [PMID:27491710]
ChEMBL	Binding affinity to recombinant human 6His-tagged SENP1 catalytic domain expressed in Escherichia coli	B	6.03	pKi	930	nM	Ki	Bioorg Med Chem Lett (2016) 26: 4237-4240 [PMID:27491710]
ChEMBL	Inhibition of human SENP1 catalytic domain assessed as reduction in deSUMOylation of RanGAP1-SUMO2 using RanGAP1-SUMO2 as substrate by fluorescence based analysis	B	5.72	pIC50	1900	nM	IC50	Eur J Med Chem (2022) 241: 114650-114650 [PMID:35939992]
ChEMBL	Inhibition of recombinant human 6His-tagged SENP1 catalytic domain expressed in Escherichia coli preincubated for 15 mins followed by addition of SUMO1-AMC as substrate measured after 1 hr by fluorescence analysis	B	5.72	pIC50	1890	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4237-4240 [PMID:27491710]
ChEMBL	Inhibition of full-length recombinant human 6His-tagged SENP1 expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins followed by addition of SUMO1-AMC as substrate measured after 1 hr by fluorescence analysis	B	5.79	pIC50	1640	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4237-4240 [PMID:27491710]
ChEMBL	Inhibition of human full-length recombinant SENP1	B	5.8	pIC50	1600	nM	IC50	Eur J Med Chem (2022) 241: 114650-114650 [PMID:35939992]
Ubiquitin carboxyl-terminal hydrolase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2406900] [UniProtKB: Q13107]
ChEMBL	Inhibition of human USP4 using Ub-AMC as substrate after 60 mins by fluorometric analysis	B	5.82	pIC50	1500	nM	IC50	Bioorg Med Chem Lett (2013) 23: 4328-4331 [PMID:23791076]
ubiquitin specific peptidase 5/Ubiquitin carboxyl-terminal hydrolase 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6158] [GtoPdb: 2431] [UniProtKB: P45974]
ChEMBL	Competitive inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate	B	4.9	pKi	12500	nM	Ki	Bioorg Med Chem Lett (2013) 23: 4328-4331 [PMID:23791076]
ChEMBL	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate after 60 mins by fluorometric analysis	B	5.4	pKi	4000	nM	Ki	Bioorg Med Chem Lett (2013) 23: 4328-4331 [PMID:23791076]
GtoPdb	-	-	5.4	pKi	4000	nM	Ki	Bioorg Med Chem Lett (2013) 23: 4328-31 [PMID:23791076]
ChEMBL	Inhibition of wild type human USP5 expressed in Escherichia coli BL21(DE3) using Ub-AMC as substrate after 60 mins by fluorometric analysis	B	5.23	pIC50	5900	nM	IC50	Bioorg Med Chem Lett (2013) 23: 4328-4331 [PMID:23791076]
ubiquitin C-terminal hydrolase L1/Ubiquitin carboxyl-terminal hydrolase isozyme L1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6159] [GtoPdb: 2426] [UniProtKB: P09936]
ChEMBL	Inhibition of human UCH-L1 using Ub-AMC as substrate after 60 mins by fluorometric analysis	B	4.65	pIC50	22300	nM	IC50	Bioorg Med Chem Lett (2013) 23: 4328-4331 [PMID:23791076]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]