ChEMBL ligand: CHEMBL504816 (1-Methyltryptophan)
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase in Human [ChEMBL: CHEMBL4685] [GtoPdb: 2829] [UniProtKB: P14902] There should be some charts here, you may need to enable JavaScript!
indoleamine 2,3-dioxygenase 1 in Human [GtoPdb: 2829] [UniProtKB: P14902] indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase 1 in Mouse [ChEMBL: CHEMBL1075294] [GtoPdb: 2829] [UniProtKB: P28776] There should be some charts here, you may need to enable JavaScript!
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Human [ChEMBL: CHEMBL3627587] [GtoPdb: 3019] [UniProtKB: Q6ZQW0] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4685] [GtoPdb: 2829] [UniProtKB: P14902]
ChEMBL	Inhibition of indoleamine 2,3-dioxygenase in aerobic condition	B	4.27	pKi	53200	nM	Ki	Bioorg Med Chem (2008) 16: 8661-8669 [PMID:18715787]
GtoPdb	-	-	4.37	pKi	42300	nM	Ki	J Med Chem (2013) 56: 8321-31 [PMID:24099220]
ChEMBL	Competitive inhibition of human recombinant IDO1 using L-tryptophan as substrate	B	4.37	pKi	42300	nM	Ki	J Med Chem (2013) 56: 8321-8331 [PMID:24099220]
ChEMBL	Inhibition of IDO	B	4.43	pKi	37000	nM	Ki	Bioorg Med Chem (2011) 19: 1550-1561 [PMID:21269836]
ChEMBL	Competitive inhibition of IDO1 (unknown origin) using L-tryptophan as substrate incubated for 30 min assessed as reduction in conversion of N-formyl kynurenine to kynurenine by dixon plot analysis	B	4.47	pKi	34000	nM	Ki	WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (2017)
ChEMBL	Competitive inhibition of human IDO1 in presence of tryptophan	B	4.47	pKi	34000	nM	Ki	J Med Chem (2013) 56: 8321-8331 [PMID:24099220]
ChEMBL	Inhibition of IDO1 (unknown origin)	B	4.47	pKi	34000	nM	Ki	Medchemcomm (2019) 10: 1740-1754 [PMID:32055299]
ChEMBL	Competitive inhibition of recombinant human IDO1 expressed in Escherichia coli BL21 cells assessed as apparent inhibition constant using varying concentration of L-tryptophan as substrate by Dixon plot analysis	B	4.47	pKi	34000	nM	Ki	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of IDO1 (unknown origin)	B	4.52	pKi	30000	nM	Ki	Eur J Med Chem (2017) 138: 199-211 [PMID:28667875]
ChEMBL	Inhibition of IDO by cell-free assay	B	4.72	pKi	19000	nM	Ki	J Med Chem (2010) 53: 1172-1189 [PMID:20055453]
ChEMBL	Competitive inhibition of histidine-tagged human recombinant IDO expressed in bacterial strain BL21 AI using L-Trptophan as substrate measured at 490 nm wavelength after 6 min by colorimetric assay	B	5.15	pKi	7000	nM	Ki	Eur J Med Chem (2011) 46: 3058-3065 [PMID:21419531]
ChEMBL	Inhibition of human indoleamine 2,3-dioxygenase expressed in Escherichia coli by spectrophotometric activity assay	B	5.18	pKi	6600	nM	Ki	J Nat Prod (2006) 69: 1496-1499 [PMID:17067170]
ChEMBL	Inhibition of recombinant N-terminal His-tagged human IDO (Ala2 to Gly403) overexpressed in Escherichia coli BL21 AI using L-tryptophan as substrate assessed as formation of kynurenine incubated for 5 mins in presence of p-dimethylaminobenzaldehyde	B	4	pIC50	100000	nM	IC50	Eur J Med Chem (2014) 82: 96-105 [PMID:24878638]
ChEMBL	Inhibition of human IDO activity by spectrophotometry	B	4	pIC50	100000	nM	IC50	Bioorg Med Chem (2011) 19: 1550-1561 [PMID:21269836]
ChEMBL	Inhibition of purified recombinant human IDO1 using L-tryptophan substrate incubated fro 30 mins	B	4.01	pIC50	98500	nM	IC50	Bioorg Med Chem (2019) 27: 3135-3144 [PMID:31178268]
ChEMBL	Inhibition of human recombinant IDO expressed in HEK293 cells assessed as blockade of tryptophan degradation by HPLC	B	4.05	pIC50	90000	nM	IC50	J Med Chem (2010) 53: 1172-1189 [PMID:20055453]
ChEMBL	Inhibition of IFNgamma-induced IDO1 in human HeLa cells using L-tryptophan as substrate after 24 hrs	B	4.11	pIC50	77290	nM	IC50	Eur J Med Chem (2017) 138: 199-211 [PMID:28667875]
ChEMBL	Inhibition of recombinant human IDO1 expressed in Escherichia coli BL21(DE3) assessed as effect on kynurenine level using L-tryptophan as substrate incubated for 1 hr by Ehrlich's reagent based assay	B	4.38	pIC50	42000	nM	IC50	J Med Chem (2020) 63: 15115-15139 [PMID:33215494]
ChEMBL	Inhibition of human recombinant IDO1 measured after 60 mins by fluorescence based analysis	B	4.38	pIC50	41740	nM	IC50	Eur J Med Chem (2023) 254: 115349-115349 [PMID:37060754]
ChEMBL	Inhibition of human IDO1 expressed in Escherichia coli BL21 Star assessed as kynurenine formation using L-Trp as substrate preincubated for 90 mins measured after 30 mins by bridge-IT fluorescence assay	B	4.46	pIC50	35000	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4330-4333 [PMID:27469130]
ChEMBL	Inhibition of human IDO1 expressed in HEK293 cells assessed as kynurenine release after 5 hrs by spectrophotometry	B	4.74	pIC50	18400	nM	IC50	J Med Chem (2013) 56: 8321-8331 [PMID:24099220]
ChEMBL	Inhibition of recombinant human IDO1 expressed in HEK293 cells assessed as reduction in kynurenine formation after 5 hrs by microplate reader analysis	B	4.74	pIC50	18400	nM	IC50	J Med Chem (2019) 62: 9161-9174 [PMID:31580660]
ChEMBL	Inhibition of IDO1 in human U87MG cells in presence of L-tryptophan incubated for 24 h	B	4.74	pIC50	18400	nM	IC50	WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (2017)
ChEMBL	Inhibition of IDO1 in human HeLa cells	B	4.74	pIC50	18400	nM	IC50	J Med Chem (2020) 63: 15115-15139 [PMID:33215494]
ChEMBL	Inhibition of IDO1 in IFN-gamma treated human HeLa cells incubated for 48 hrs by absorbance based analysis	B	4.83	pIC50	14690	nM	IC50	Eur J Med Chem (2023) 254: 115349-115349 [PMID:37060754]
ChEMBL	Inhibition of His-tagged recombinant human IDO1 expressed in Escherichia coli using tryptophan as substrate by spectrophotometric analysis	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem (2012) 20: 1354-1363 [PMID:22112538]
ChEMBL	Inhibition of N-terminus 6xHis-tagged human IDO1 expressed in Escherichia coli M15 using L-tryptophan as substrate preincubated for 1 hr measured after 15 mins in presence of bovine liver and methylene blue by HPLC analysis	B	7.11	pIC50	78	nM	IC50	ACS Med Chem Lett (2016) 7: 1167-1172 [PMID:27994758]
indoleamine 2,3-dioxygenase 1 in Human [GtoPdb: 2829] [UniProtKB: P14902]
GtoPdb	-	-	4.37	pKi	42300	nM	Ki	J Med Chem (2013) 56: 8321-31 [PMID:24099220]
indoleamine 2,3-dioxygenase 1/Indoleamine 2,3-dioxygenase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075294] [GtoPdb: 2829] [UniProtKB: P28776]
ChEMBL	Inhibition of mouse recombinant IDO expressed in mouse P815B cells assessed as blockade of tryptophan degradation by HPLC	B	4.4	pIC50	40000	nM	IC50	J Med Chem (2010) 53: 1172-1189 [PMID:20055453]
indoleamine 2,3-dioxygenase 2/Indoleamine 2,3-dioxygenase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3627587] [GtoPdb: 3019] [UniProtKB: Q6ZQW0]
ChEMBL	Inhibition of IDO2 (unknown origin) expressed in human U87MG cells in presence of L-tryptophan incubated for 24 h	B	4.08	pIC50	82530	nM	IC50	WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (2017)
ChEMBL	Inhibition of recombinant human C-terminal His6-tagged IDO2 (14-420 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in L-kynurenine formation using L-tryptophan as substrate after 30 mins in presence of catalase by methylene blue dye based assay	B	4.08	pIC50	82500	nM	IC50	Eur J Med Chem (2016) 123: 171-179 [PMID:27475108]
ChEMBL	Inhibition of human IDO2 using L-tryptophan as substrate assessed as reduction in conversion of N-formyl kynurenine to kynurenine	B	4.24	pIC50	56960	nM	IC50	WO-2017114260-A1. Uses of tryptanthrin and derivative thereof in preparing hldo2 inhibitor (2017)
ChEMBL	Inhibition of recombinant human IDO2 expressed in human U87MG cells assessed as reduction in kynurenine formation using L-tryptophan as substrate after 6 hrs by spectrophotometry	B	4.24	pIC50	56900	nM	IC50	Eur J Med Chem (2016) 123: 171-179 [PMID:27475108]
ChEMBL	Inhibition of human IDO2 expressed in Escherichia coli BL21 Star assessed as kynurenine formation using L-Trp as substrate preincubated for 90 mins measured after 30 mins by bridge-IT fluorescence assay	B	4.46	pIC50	35000	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4330-4333 [PMID:27469130]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]