ChEMBL ligand: CHEMBL796 (Concerta, Cotempla xr-odt, Daytrana, Methylin, Methylphenidate, Methylphenidate extended release, Metilfenidato, Prc-063, Threo-dl-methylphenidate)
DAT/Dopamine transporter in Human [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959] DAT/Dopamine transporter in Rat [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977] There should be some charts here, you may need to enable JavaScript!
NET/Norepinephrine transporter in Human [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975] There should be some charts here, you may need to enable JavaScript!
SERT/Serotonin transporter in Human [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
DAT/Dopamine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL238] [GtoPdb: 927] [UniProtKB: Q01959]
ChEMBL	Displacement of [125I]RTI-55 from human DAT expressing HEK293 cells	B	6.96	pKi	110	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]WIN 35,428 from human DAT expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	7.47	pKi	34	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
GtoPdb	-	-	7.1	pIC50	79	nM	IC50	J Med Chem (2007) 50: 219-32 [PMID:17228864]
ChEMBL	Inhibition of [3H]DA uptake at human DAT expressing HEK293 cells	B	7.1	pIC50	79	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Inhibition of [3H]WIN-35428 Binding to the Dopamine Transporter in Rhesus (Macaca mulatta) orCynomolgus Monkey (Macaca fascicularis) Caudate-Putamen	B	7.77	pIC50	17	nM	IC50	J Med Chem (2003) 46: 1538-1545 [PMID:12672255]
DAT/Dopamine transporter in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL338] [GtoPdb: 927] [UniProtKB: P23977]
ChEMBL	Tested for the ability to bind stereoselectively and enantioselectively to the dopamine transporter (DAT); Value ranges from 34 - 83 nM	B	7.08	pIC50	83	nM	IC50	J Med Chem (2003) 46: 1538-1545 [PMID:12672255]
NET/Norepinephrine transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL222] [GtoPdb: 926] [UniProtKB: P23975]
ChEMBL	Displacement of [125I]RTI-55 from human NET expressing HEK293 cells	B	6.18	pKi	660	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]Nisoxetine from human NET expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	6.47	pKi	340	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
GtoPdb	-	-	7.21	pIC50	61	nM	IC50
ChEMBL	Inhibition of [3H]NE uptake into human NET expressing HEK293 cells	B	7.21	pIC50	61	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]
SERT/Serotonin transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL228] [GtoPdb: 928] [UniProtKB: P31645]
ChEMBL	Displacement of [125I]RTI-55 from human SERT expressing HEK293 cells	B	4.19	pKi	65000	nM	Ki	J Med Chem (2007) 50: 219-232 [PMID:17228864]
ChEMBL	Displacement of [3H]Citolapram from human SERT expressed in HEK293E cells after 1 hr by liquid scintillation counting	B	5	pKi	>10000	nM	Ki	ACS Med Chem Lett (2014) 5: 760-765 [PMID:25050161]
ChEMBL	Inhibition of [3H]5-HT uptake into human SERT expressing HEK293 cells	B	5.29	pIC50	5100	nM	IC50	J Med Chem (2007) 50: 219-232 [PMID:17228864]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]