ChEMBL ligand: CHEMBL2364628 (ACY-1215, ACY-63, Ricolinostat)
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 10/Histone deacetylase 10 in Human [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 11/Histone deacetylase 11 in Human [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 2/Histone deacetylase 2 in Human [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3 in Human [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 4/Histone deacetylase 4 in Human [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 5/Histone deacetylase 5 in Human [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase 6 in Danio rerio [ChEMBL: CHEMBL4739666] [UniProtKB: F8W336] histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 7/Histone deacetylase 7 in Human [ChEMBL: CHEMBL2716] [GtoPdb: 2661] [UniProtKB: Q8WUI4] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 8/Histone deacetylase 8 in Human [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 9/Histone deacetylase 9 in Human [ChEMBL: CHEMBL4145] [GtoPdb: 2620] [UniProtKB: Q9UKV0] There should be some charts here, you may need to enable JavaScript!
Leukotriene A4 hydrolase/Leukotriene A4 hydrolase in Human [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960] There should be some charts here, you may need to enable JavaScript!
sirtuin 1/NAD-dependent deacetylase sirtuin 1 in Human [ChEMBL: CHEMBL4506] [GtoPdb: 2707] [UniProtKB: Q96EB6] There should be some charts here, you may need to enable JavaScript!
sirtuin 2/NAD-dependent deacetylase sirtuin 2 in Human [ChEMBL: CHEMBL4462] [GtoPdb: 2708] [UniProtKB: Q8IXJ6] There should be some charts here, you may need to enable JavaScript!
proteasome 20S subunit beta 5/Proteasome Macropain subunit MB1 in Human [ChEMBL: CHEMBL4662] [GtoPdb: 2406] [UniProtKB: P28074] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Binding affinity to recombinant HDAC1 (unknown origin) assessed as inhibition constant using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	7.13	pKi	74	nM	Ki	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant human HDAC1 using Boc-Lys-(acetyl)-AMC as substrate incubated for 0.5 hr and measured after 20 mins by fluorescence assay	B	6.41	pIC50	390	nM	IC50	Eur J Med Chem (2022) 233: 114228-114228 [PMID:35245830]
ChEMBL	Inhibition of human recombinant HDAC1 using ZMAL (Z-Lys(Ac)-AMC) fluorogenic substrate incubated for 90 mins by fluorescence based assay	B	6.72	pIC50	190	nM	IC50	J Med Chem (2020) 63: 10339-10351 [PMID:32803970]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	6.73	pIC50	187.9	nM	IC50	Medchemcomm (2019) 10: 1109-1115 [PMID:31391882]
ChEMBL	Inhibition of recombinant HDAC1 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	6.89	pIC50	130	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7	pIC50	100	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG-tagged HDAC1 expressed in sf9 cells preincubated for 5 mins followed by biotinylated H3K9-Ac substrate addition and measured after 60 mins by HTRF assay	B	7.13	pIC50	74	nM	IC50	Bioorg Med Chem Lett (2019) 29: 349-352 [PMID:30594434]
ChEMBL	Inhibition of HDAC1 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	7.24	pIC50	58	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC1 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition by microtiter plate reader assay	B	7.24	pIC50	58	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac) (379 to 382) p53 peptide as substrate by fluorescence assay	B	7.24	pIC50	58	nM	IC50	J Med Chem (2014) 57: 4009-4022 [PMID:24766560]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-His-tagged HDAC1 expressed in Sf9 cells using FTS as substrate preincubated for 10 mins followed by substrate addition measured over 30 mins	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2017) 127: 115-127 [PMID:28038324]
ChEMBL	Inhibition of HADC1 (unknown origin)	B	7.24	pIC50	58	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of recombinant human full length C-terminal His/FLAG-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells assessed as decrease in release of 7-amino-4-methoxycoumarin using FTS as substrate preincubated for 10 mins followed by substrate addition measured after 60 sec by microplate reader assay	B	7.24	pIC50	58	nM	IC50	J Med Chem (2019) 62: 8557-8577 [PMID:31414801]
ChEMBL	Inhibition of HDAC1 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorescent peptide substrate	B	7.24	pIC50	58	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorophore tripeptide as substrate after 30 mins	B	7.24	pIC50	58	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
GtoPdb	-	-	7.24	pIC50	58	nM	IC50	Blood (2012) 119: 2579-89 [PMID:22262760]
ChEMBL	Inhibition of HDAC1 (unknown origin) using FTS as substrate preincubated for 6 mins followed by substrate addition measured after 60 secs	B	7.28	pIC50	53	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of human recombinant HDAC1 using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	7.42	pIC50	38	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of human HDAC1 preincubated for 15 mins followed by substrate addition by fluorescence-based assay	B	7.72	pIC50	19	nM	IC50	Eur J Med Chem (2021) 218: 113383-113383 [PMID:33799069]
ChEMBL	Inhibition of HDAC1 (unknown origin) measured by fluorescence based assay	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
histone deacetylase 10/Histone deacetylase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8]
ChEMBL	Inhibition of HDAC10 (unknown origin)	B	5	pIC50	>10000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of dye-labeled tracer binding to HDAC10 (unknown origin) transfected in human HeLa cells measured after 2 hrs by nano-luciferase reporter gene-based BRET assay	B	5.8	pIC50	1584.89	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
ChEMBL	Inhibition of recombinant HDAC10 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	6.71	pIC50	194	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of tubastatin-Alexa647-tracer binding to recombinant GST-tagged HDAC10 (unknown origin) measured after 1 hr by TR-FRET assay	B	6.9	pIC50	125.89	nM	IC50	J Med Chem (2019) 62: 4426-4443 [PMID:30964290]
histone deacetylase 11/Histone deacetylase 11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2]
ChEMBL	Inhibition of recombinant HDAC11 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC11 (unknown origin) using fluorescent peptide substrate	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC11 (unknown origin)	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC11 (unknown origin) measured by fluorescence based assay	B	5.3	pIC50	>5000	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	Inhibition of recombinant human HDAC11 using fluorogenic HDAC substrate class 2a incubated for 30 mins by fluorimetry	B	6	pIC50	>1000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of recombinant full length HDAC11 (unknown origin) expressed in insect Sf9 cells by EMSA analysis	B	6	pIC50	>1000	nM	IC50	J Med Chem (2021) 64: 8486-8509 [PMID:34101461]
ChEMBL	Inhibition of full length human recombinant HDAC11 expressed in Sf9 baculovirus system using FAM-labeled acetylated peptide as substrate by measuring fluorescence intensity by EMSA method	B	6	pIC50	>1000	nM	IC50	J Med Chem (2022) 65: 3193-3217 [PMID:35119267]
ChEMBL	Inhibition of human HDAC11 using fluorogenic HDAC substrate incubated for 30 mins by fluorimetry	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of recombinant full length human HDAC11 expressed in SF9 baculovirus using FAM- labelled acetylated peptide as substrate measured by Electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2020) 201: 112411-112411 [PMID:32615502]
histone deacetylase 2/Histone deacetylase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769]
ChEMBL	Binding affinity to recombinant HDAC2 (unknown origin) assessed as inhibition constant using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	6.69	pKi	204	nM	Ki	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	6.39	pIC50	403	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in SF9 baculovirus using FAM- labelled acetylated peptide as substrate measured by Electrophoretic mobility shift assay	B	6.42	pIC50	379	nM	IC50	Eur J Med Chem (2020) 201: 112411-112411 [PMID:32615502]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in baculovirus infected Sf9 cells using FAM-RHKK-Ac as substrate incubated for 17 hrs by electrophoretic mobility shift assay	B	6.42	pIC50	379	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of recombinant HDAC2 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	7.02	pIC50	95	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of full length recombinant human C-terminal His-tagged HDAC2 expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7.18	pIC50	66	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
ChEMBL	Inhibition of human HDAC2 expressed in Sf9 cells using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	7.32	pIC50	48	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC2 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition by microtiter plate reader assay	B	7.32	pIC50	48	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of HDAC2 (unknown origin) using FTS as substrate preincubated for 6 mins followed by substrate addition measured after 60 secs	B	7.32	pIC50	48	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorescent peptide substrate	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC2 (unknown origin) using fluorophore tripeptide as substrate after 30 mins	B	7.32	pIC50	48	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of HDAC2 (unknown origin)	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKK(Ac) (379 to 382) p53 peptide as substrate by fluorescence assay	B	7.32	pIC50	48	nM	IC50	J Med Chem (2014) 57: 4009-4022 [PMID:24766560]
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-tagged HDAC2 expressed in Sf9 cells using FTS as substrate preincubated for 10 mins followed by substrate addition measured over 30 mins	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2017) 127: 115-127 [PMID:28038324]
ChEMBL	Inhibition of HADC2 (unknown origin)	B	7.32	pIC50	48	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of human HDAC2 expressed in Sf9 insect cells using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs	B	7.32	pIC50	48	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
GtoPdb	-	-	7.32	pIC50	48	nM	IC50	Blood (2012) 119: 2579-89 [PMID:22262760]
histone deacetylase 3/Histone deacetylase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379]
ChEMBL	Binding affinity to recombinant HDAC3 (unknown origin) assessed as inhibition constant using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	6.91	pKi	123	nM	Ki	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant HDAC3 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	6.53	pIC50	295	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant HDAC3 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	6.87	pIC50	135	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
GtoPdb	-	-	7.29	pIC50	51	nM	IC50	Blood (2012) 119: 2579-89 [PMID:22262760]
ChEMBL	Inhibition of HDAC3 (unknown origin)	B	7.29	pIC50	51	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of recombinant HDAC3 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based assay	B	7.29	pIC50	51	nM	IC50	J Med Chem (2021) 64: 3794-3812 [PMID:33769811]
ChEMBL	Inhibition of HDAC3 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate and trypsin addition by microplate reader based assay	B	7.29	pIC50	51	nM	IC50	J Med Chem (2021) 64: 3794-3812 [PMID:33769811]
ChEMBL	Inhibition of HDAC3 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition by microtiter plate reader assay	B	7.29	pIC50	51	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of HDAC3 (unknown origin)	B	7.29	pIC50	51	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HADC3 (unknown origin)	B	7.29	pIC50	51	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of HDAC3 (unknown origin) using FTS as substrate preincubated for 6 mins followed by substrate addition measured after 60 secs	B	7.29	pIC50	51	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of HDAC3 (unknown origin) using fluorescent peptide substrate	B	7.29	pIC50	51	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC3 (unknown origin) using fluorophore tripeptide as substrate after 30 mins	B	7.29	pIC50	51	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of recombinant full length human HDAC3 expressed in SF9 baculovirus using FAM- labelled acetylated peptide as substrate measured by Electrophoretic mobility shift assay	B	7.84	pIC50	14.3	nM	IC50	Eur J Med Chem (2020) 201: 112411-112411 [PMID:32615502]
ChEMBL	Inhibition of full length human recombinant HDAC3 expressed in Sf9 baculovirus system using FAM-labeled acetylated peptide as substrate by measuring fluorescence intensity by EMSA method	B	7.84	pIC50	14.3	nM	IC50	J Med Chem (2022) 65: 3193-3217 [PMID:35119267]
ChEMBL	Inhibition of recombinant full length human HDAC3 expressed in insect Sf9 cells by EMSA analysis	B	7.84	pIC50	14.3	nM	IC50	J Med Chem (2021) 64: 8486-8509 [PMID:34101461]
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618]
GtoPdb	-	-	7.29	pIC50	51	nM	IC50	Blood (2012) 119: 2579-89 [PMID:22262760]
ChEMBL	Inhibition of human C-terminal His-tagged HDAC3 (1 to 428 residues)/human N-terminal GST-tagged NcoR2 (395 to 489 residues) expressed in sf9 cells using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	7.29	pIC50	51	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of full length recombinant human C-terminal His-tagged HDAC3/N-terminal GST-tagged NCOR2 (395 to 489 residues) expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	7.43	pIC50	37	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
histone deacetylase 4/Histone deacetylase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524]
ChEMBL	Inhibition of recombinant HDAC4 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC4 (unknown origin) using tripeptide MAZ-1675 as substrate after 30 mins	B	5.15	pIC50	7000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of HDAC4 (unknown origin)	B	5.15	pIC50	7000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC4 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition by microtiter plate reader assay	B	5.15	pIC50	7000	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of HDAC4 (unknown origin) using fluorescent peptide substrate	B	5.15	pIC50	7000	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC4 (unknown origin)	B	5.15	pIC50	7000	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of recombinant HDAC4 (unknown origin) using Boc-Lys (trifluoroacetyl) AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of recombinant full length human HDAC4 expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 1.5 hrs by electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of HDAC4 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of recombinant full length human HDAC4 expressed in SF9 baculovirus using FAM- labelled acetylated peptide as substrate measured by Electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2020) 201: 112411-112411 [PMID:32615502]
ChEMBL	Inhibition of HDAC4 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	6	pIC50	>1000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
histone deacetylase 5/Histone deacetylase 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6]
ChEMBL	Inhibition of recombinant HDAC5 (unknown origin) using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of HDAC5 (unknown origin)	B	5.3	pIC50	5000	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC5 (unknown origin) using fluorescent peptide substrate	B	5.3	pIC50	5000	nM	IC50	Eur J Med Chem (2022) 229: 114090-114090 [PMID:34992037]
ChEMBL	Inhibition of HDAC5 (unknown origin) using tripeptide MAZ-1675 as substrate after 30 mins	B	5.3	pIC50	5000	nM	IC50	J Med Chem (2013) 56: 6297-6313 [PMID:23627282]
ChEMBL	Inhibition of HDAC5 (unknown origin)	B	5.3	pIC50	5000	nM	IC50	Eur J Med Chem (2021) 221: 113526-113526 [PMID:33992929]
ChEMBL	Inhibition of HDAC5 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition by microtiter plate reader assay	B	5.3	pIC50	5000	nM	IC50	J Med Chem (2021) 64: 1362-1391 [PMID:33523672]
ChEMBL	Inhibition of recombinant HDAC5 (unknown origin) using Boc-Lys (trifluoroacetyl) AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	5.3	pIC50	>5000	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of HDAC5 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs	B	6	pIC50	>1000	nM	IC50	Eur J Med Chem (2018) 143: 1406-1418 [PMID:29133060]
ChEMBL	Inhibition of HDAC5 (unknown origin) using MAZ-1675 as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	6	pIC50	>1000	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
Histone deacetylase 6 in Danio rerio (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4739666] [UniProtKB: F8W336]
ChEMBL	Inhibition of N-terminal 6 His-SUMO tagged Zebrafish HDAC6 CD2 domain expressed in Escherichia coli BL21(DE3) RILP pretreated for 10 mins before FITC-M344 addition and measured after 10 mins by fluorescence polarization competition assay	B	7.05	pIC50	90	nM	IC50	Eur J Med Chem (2020) 201: 112411-112411 [PMID:32615502]
histone deacetylase 6/Histone deacetylase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7]
ChEMBL	Binding affinity to recombinant HDAC6 (unknown origin) assessed as inhibition constant using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	9	pKi	1	nM	Ki	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of full length human HDAC6 using FAM-labeled acetylated peptide as substrate by electrophoretic mobility shift assay	B	5.59	pIC50	2600	nM	IC50	J Med Chem (2021) 64: 2691-2704 [PMID:33576627]
ChEMBL	Enzyme Assays: Compounds for testing were diluted in DMSO to 50 fold the final concentration and a ten point three fold dilution series was made. The compounds were diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, 20 uM TCEP) to 6 fold their final concentration. The HDAC enzymes (purchased from BPS Biosciences) were diluted to 1.5 fold their final concentration in assay buffer. The tripeptide substrate and trypsin at 0.05 uM final concentration were diluted in assay buffer at 6 fold their final concentration. The final enzyme concentrations used in these assays were 3.3 ng/ml (HDAC1), 0.2 ng/ml (HDAC2), 0.08 ng/ml (HDAC3) and 2 ng/ml (HDAC6). The final substrate concentrations used were 16 uM (HDAC1), 10 uM (HDAC2), 17 uM (HDAC3) and 14 uM (HDAC6).	B	7.08	pIC50	84	nM	IC50	US-8609678-B2. Reverse amide compounds as protein deacetylase inhibitors and methods of use thereof (2013)
ChEMBL	Inhibition of recombinant human HDAC6 using Boc-Lys-(acetyl)-AMC as substrate incubated for 0.5 hr and measured after 20 mins by fluorescence assay	B	7.54	pIC50	29	nM	IC50	Eur J Med Chem (2022) 233: 114228-114228 [PMID:35245830]
ChEMBL	Inhibition of human HDAC6 (unknown origin) using RHKKAc as substrate by fluorescence method	B	7.57	pIC50	27	nM	IC50	Bioorg Med Chem Lett (2021) 34: 127763-127763 [PMID:33359604]
ChEMBL	Inhibition of human recombinant HDAC6 using ZMAL (Z-Lys(Ac)-AMC) fluorogenic substrate incubated for 90 mins by fluorescence based assay	B	7.7	pIC50	20	nM	IC50	J Med Chem (2020) 63: 10339-10351 [PMID:32803970]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	7.75	pIC50	17.9	nM	IC50	Medchemcomm (2019) 10: 1109-1115 [PMID:31391882]
ChEMBL	Inhibition of HDAC6 (unknown origin) measured by fluorescence based assay	B	7.77	pIC50	17	nM	IC50	Bioorg Med Chem (2022) 73: 117028-117028 [PMID:36182802]
ChEMBL	Inhibition of recombinant full length human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluorescence method	B	7.92	pIC50	12	nM	IC50	J Med Chem (2019) 62: 10711-10739 [PMID:31710483]
ChEMBL	Enzyme Assays: Compounds for testing were diluted in DMSO to 50 fold the final concentration and a ten point three fold dilution series was made. The compounds were diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, 20 uM TCEP) to 6 fold their final concentration. The HDAC enzymes (purchased from BPS Biosciences) were diluted to 1.5 fold their final concentration in assay buffer. The tripeptide substrate and trypsin at 0.05 uM final concentration were diluted in assay buffer at 6 fold their final concentration. The final enzyme concentrations used in these assays were 3.3 ng/ml (HDAC1), 0.2 ng/ml (HDAC2), 0.08 ng/ml (HDAC3) and 2 ng/ml (HDAC6). The final substrate concentrations used were 16 uM (HDAC1), 10 uM (HDAC2), 17 uM (HDAC3) and 14 uM (HDAC6).	B	8	pIC50	10	nM	IC50	US-8609678-B2. Reverse amide compounds as protein deacetylase inhibitors and methods of use thereof (2013)
ChEMBL	Inhibition of full length recombinant human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 cells using Boc-Lys-(Ac)-AMC as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins by fluorescence assay	B	8.05	pIC50	9	nM	IC50	Bioorg Med Chem (2018) 26: 1418-1425 [PMID:29500130]
ChEMBL	Inhibition of human recombinant HDAC6 using AMC labeled AC-peptide as substrate incubated for 1 hr by fluorescence analysis	B	8.05	pIC50	9	nM	IC50	J Med Chem (2016) 59: 1455-1470 [PMID:26443078]
ChEMBL	Inhibition of recombinant HDAC6 (unknown origin) using Boc-Lys(acetyl)-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence based microplate reader analysis	B	8.15	pIC50	7	nM	IC50	J Med Chem (2022) 65: 12140-12162 [PMID:36073117]
ChEMBL	Inhibition of human HDAC6 preincubated for 15 mins followed by substrate addition by fluorescence-based assay	B	8.17	pIC50	6.8	nM	IC50	Eur J Med Chem (2021) 218: 113383-113383 [PMID:33799069]
ChEMBL	Inhibition of full length recombinant human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 cells preincubated for 5 mins followed by biotinylated H3K9-Ac substrate addition and measured after 60 mins by HTRF assay	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2019) 29: 349-352 [PMID:30594434]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	8.3	pIC50	5	nM	IC50	Eur J Med Chem (2022) 229: 114053-114053 [PMID:34974338]
ChEMBL	Inhibition of HDAC6 (unknown origin) using FTS as substrate preincubated for 10 mins followed by substrate addition and shaken for 60 secs and measured over 30 mins	B	8.3	pIC50	5	nM	IC50	J Med Chem (2020) 63: 12460-12484 [PMID:32608981]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	8.33	pIC50	4.7	nM	IC50	Eur J Med Chem (2021) 209: 112887-112887 [PMID:33035922]
ChEMBL	Inhibition of HDAC6 (unknown origin) using RHKK(Ac) (379 to 382) p53 peptide as substrate by fluorescence assay	B	8.33	pIC50	4.7	nM	IC50	J Med Chem (2014) 57: 4009-4022 [PMID:24766560]
ChEMBL	Inhibition of full length human recombinant N-terminal GST-tagged HDAC6 expressed in Sf9 cells using FTS as substrate preincubated for 10 mins followed by substrate addition measured over 30 mins	B	8.33	pIC50	4.7	nM	IC50	Eur J Med Chem (2017) 127: 115-127 [PMID:28038324]
ChEMBL