ChEMBL ligand: CHEMBL99 (A-300-I, ANTIBIOTIC A-300, GNF-Pf-1011, Trichostatin, Trichostatin(s))
coagulation factor III, tissue factor/Coagulation factor III in Human [ChEMBL: CHEMBL4081] [GtoPdb: 3192] [UniProtKB: P13726] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase in Plasmodium falciparum [ChEMBL: CHEMBL5541] [UniProtKB: Q9XYC7] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 1/Histone deacetylase 1 in Human [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547] histone deacetylase 1/Histone deacetylase 1 in Mouse [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 10/Histone deacetylase 10 in Human [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 11/Histone deacetylase 11 in Human [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 2/Histone deacetylase 2 in Human [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3 in Human [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/NCoR1 in Human [ChEMBL: CHEMBL3038484] [GtoPdb: 2617] [UniProtKB: O15379, O75376] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 4/Histone deacetylase 4 in Human [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 5/Histone deacetylase 5 in Human [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6] histone deacetylase 5/Histone deacetylase 5 in Mouse [ChEMBL: CHEMBL2768] [GtoPdb: 2660] [UniProtKB: Q9Z2V6] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] histone deacetylase 6/Histone deacetylase 6 in Mouse [ChEMBL: CHEMBL2878] [GtoPdb: 2618] [UniProtKB: Q9Z2V5] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 7/Histone deacetylase 7 in Human [ChEMBL: CHEMBL2716] [GtoPdb: 2661] [UniProtKB: Q8WUI4] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase 8 in Schistosoma mansoni [ChEMBL: CHEMBL3797017] [UniProtKB: A5H660] histone deacetylase 8/Histone deacetylase 8 in Human [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41] histone deacetylase 8/Histone deacetylase 8 in Mouse [ChEMBL: CHEMBL2347] [GtoPdb: 2619] [UniProtKB: Q8VH37] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 9/Histone deacetylase 9 in Human [ChEMBL: CHEMBL4145] [GtoPdb: 2620] [UniProtKB: Q9UKV0] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase HD1B in Zea mays [ChEMBL: CHEMBL3541] [UniProtKB: Q9ZTP8] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase HD2 in Zea mays [ChEMBL: CHEMBL4919] [UniProtKB: Q94F81] There should be some charts here, you may need to enable JavaScript!
Histone deacetylase-like amidohydrolase in Alcaligenes sp. (strain DSM 11172) (Bordetella sp. (strain FB188)) [ChEMBL: CHEMBL6017] [UniProtKB: Q70I53] There should be some charts here, you may need to enable JavaScript!
Leukotriene A4 hydrolase/Leukotriene A4 hydrolase in Human [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960] There should be some charts here, you may need to enable JavaScript!
lysine demethylase 4E/Lysine-specific demethylase 4D-like in Human [ChEMBL: CHEMBL1293226] [GtoPdb: 2679] [UniProtKB: B2RXH2] There should be some charts here, you may need to enable JavaScript!
sirtuin 6/NAD-dependent protein deacetylase sirtuin-6 in Human [ChEMBL: CHEMBL2163182] [GtoPdb: 2712] [UniProtKB: Q8N6T7] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Splicing factor 3B subunit 3 in Human [ChEMBL: CHEMBL1250378] [UniProtKB: Q15393] There should be some charts here, you may need to enable JavaScript!
Vitamin D receptor in Human [ChEMBL: CHEMBL1977] [GtoPdb: 605] [UniProtKB: P11473] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
coagulation factor III, tissue factor/Coagulation factor III in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4081] [GtoPdb: 3192] [UniProtKB: P13726]
ChEMBL	Inhibition of LPS-induced tissue factor activity in human PBMC preincubated for 4 hrs assessed after 5 hrs of LPS challenge by one stage clotting assay	B	7.3	pIC50	50	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
ChEMBL	Inhibition of IL-1-beta-induced tissue factor activity in human PBMC preincubated for 4 hrs assessed after 5 hrs of IL1-beta challenge by one stage clotting assay	B	7.3	pIC50	50	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
ChEMBL	Inhibition of TNF-alpha-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of TNFalpha challenge by one stage clotting assay	B	7.52	pIC50	30	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
ChEMBL	Inhibition of LPS-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of LPS challenge by one stage clotting assay	B	7.7	pIC50	20	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
ChEMBL	Inhibition of IL-1-beta-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of IL1-beta challenge by one stage clotting assay	B	7.7	pIC50	20	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
ChEMBL	Inhibition of HOSCN-induced tissue factor activity in HUVEC preincubated for 4 hrs assessed after 4 hrs of HOSCN challenge by one stage clotting assay	B	7.7	pIC50	20	nM	IC50	J Biol Chem (2007) 282: 28408-28418 [PMID:17675290]
Histone deacetylase in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5541] [UniProtKB: Q9XYC7]
ChEMBL	Inhibition of Plasmodium falciparum HDAC1 expressed in Drosophila melanogaster S2 cells	B	9.22	pIC50	0.6	nM	IC50	J Med Chem (2009) 52: 2185-2187 [PMID:19317450]
histone deacetylase 1/Histone deacetylase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL325] [GtoPdb: 2658] [UniProtKB: Q13547]
ChEMBL	Binding affinity to human HDAC1 assessed as dissociation constant	B	7.82	pKd	15	nM	Kd	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of human recombinant HDAC1 by Michaelis-Menten equation analysis	B	9.06	pKi	0.87	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
ChEMBL	Inhibition of recombinant HDAC1	B	9.64	pKi	0.23	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
ChEMBL	Inhibition of human HDAC1	B	9.7	pKi	0.2	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC1	B	9.7	pKi	0.2	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	9.7	pKi	0.2	nM	Ki	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
GtoPdb	-	-	9.7	pKi	0.2	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibitory activity on partially purified recombinant human Histone deacetylase 1 (HDAC-1)	B	4.6	pIC50	>25000	nM	IC50	J Med Chem (2003) 46: 820-830 [PMID:12593661]
ChEMBL	Inhibition of HDAC1 by fluorescence assay	B	5.41	pIC50	3900	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of HDAC1 expressed in 293T cells	B	6.72	pIC50	190	nM	IC50	Bioorg Med Chem (2008) 16: 437-445 [PMID:17900911]
ChEMBL	Inhibition of human full-length recombinant HDAC1 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.21	pIC50	62	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of human HDAC1 expressed in 293T cells	B	7.44	pIC50	36.6	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2982-2984 [PMID:18397826]
ChEMBL	Inhibition of recombinant full length C-terminal FLAG/His-tagged human HDAC1 (1 to 482 residues) expressed in sf21 cells using RHK-K(Ac)-AMC as substrate by fluorimetric assay	B	7.49	pIC50	32	nM	IC50	Eur J Med Chem (2019) 162: 321-333 [PMID:30448419]
ChEMBL	Inhibition of HDAC1	B	7.59	pIC50	26	nM	IC50	Bioorg Med Chem Lett (2007) 17: 1558-1561 [PMID:17257837]
ChEMBL	Inhibition of human HDAC1 using RHKKAc as substrate by fluorescence assay	B	7.6	pIC50	24.9	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibition of human HDAC1 using fluorescent substrate Ac-KGLGK(Ac)-MCA after 30 mins by fluorescence plate reader	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem (2014) 22: 3850-3855 [PMID:24997578]
ChEMBL	Inhibition of HDAC1 by fluorimetric assay	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6770-6772 [PMID:23021104]
ChEMBL	Inhibition of recombinant human HDAC1 expressed in HEK293T cells using Ac-KGLGK(Ac)-MCA as substrate measured after 30 mins by fluorescence assay	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC1	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2010) 20: 997-999 [PMID:20045316]
ChEMBL	Inhibition of HDAC1 (unknown origin) assessed as inhibition of fluorogenic peptide deacetylation	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5222-5228 [PMID:27717544]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in NIH3T3 cells	B	7.68	pIC50	21	nM	IC50	J Med Chem (2007) 50: 5425-5438 [PMID:17929798]
ChEMBL	Inhibition of HDAC1	B	7.68	pIC50	21	nM	IC50	J Med Chem (2006) 49: 4809-4812 [PMID:16884291]
ChEMBL	Inhibition of human histone deacetylase 1 prepared from 293T cells	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2004) 14: 5343-5346 [PMID:15454224]
ChEMBL	Inhibitory concentration against histone deacetylase 1	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4427-4431 [PMID:16137884]
ChEMBL	Inhibition of recombinant human HDAC1 expressed in HEK293T cells using Ac-KGLGK(Ac)-MCA as substrate after 30 mins in presence of DTT by fluorescence assay	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem (2018) 26: 1920-1928 [PMID:29519604]
ChEMBL	Inhibition of HDAC1 in human SHSY5Y cells using MOCPAC as substrate after 8 hrs by UHPLC-ESI-MS/MS analysis	B	7.73	pIC50	18.8	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf21 insect cells by using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	7.75	pIC50	17.9	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of HDAC1 by fluorescent activity assay	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem Lett (2011) 21: 5735-5738 [PMID:21875796]
ChEMBL	Inhibition of human HDAC1	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem Lett (2007) 17: 4895-4900 [PMID:17588744]
ChEMBL	Inhibition of recombinant full length human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in baculovirus infected Sf21 cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorimetric assay	B	7.8	pIC50	15.8	nM	IC50	Eur J Med Chem (2020) 187: 111915-111915 [PMID:31838329]
ChEMBL	Inhibition of human recombinant GST-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells using MOCPAC as substrate after 4 hrs by UHPLC-ESI-MS/MS analysis	B	7.8	pIC50	15.7	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of human KDAC1 using [3H]acetyl histone H4 peptide substrate incubated for 60 mins by scintillation counting method	B	7.82	pIC50	15	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of HDAC1 (unknown origin) using fluorogenic peptide as the substrate containing residues 379-382 of p53 pre-incubated for 15 mins followed by substrate addition incubated for 30 mins by multimode plate reader	B	7.85	pIC50	14.14	nM	IC50	Bioorg Med Chem (2023) 93: 117444-117444 [PMID:37611334]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Fluor de Lys substrate by fluorescence assay relative to control	B	7.9	pIC50	12.5	nM	IC50	Eur J Med Chem (2019) 168: 110-122 [PMID:30802729]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac)AMC as substrate after 1 to 2 hrs by fluorescence assay	B	7.91	pIC50	12.44	nM	IC50	Eur J Med Chem (2018) 143: 2005-2014 [PMID:29150335]
ChEMBL	Inhibition of human HDAC1 by ELISA	B	7.92	pIC50	12	nM	IC50	Eur J Med Chem (2018) 148: 73-85 [PMID:29454918]
ChEMBL	Inhibition of human HDAC1 using RHKKAc peptide as substrate	B	7.92	pIC50	12	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of human HDAC1 after 30 mins by fluorescence assay	B	7.92	pIC50	12	nM	IC50	J Nat Prod (2012) 75: 285-289 [PMID:22276693]
ChEMBL	Inhibition of HDAC1 by fluorometric assay	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6200-6204 [PMID:22932316]
ChEMBL	Inhibition of human HDAC1 by fluorescence assay	B	7.96	pIC50	10.9	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of recombinant human HDAC1 using RHK-K(Ac) as substrate by homogeneous fluorescence release assay	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
ChEMBL	Inhibition of recombinant human HDAC1 using Cbz-Lys(TFAc)-AMC as substrate by fluorometric analysis	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2010) 20: 7067-7070 [PMID:20947351]
ChEMBL	Inhibition of recombinant human full-length C-terminal Flag-His6-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using fluorogenic HDAC substrate measured after 30 mins by fluorimetry	B	8	pIC50	<10	nM	IC50	Bioorg Med Chem (2022) 56: 116599-116599 [PMID:35041998]
ChEMBL	Inhibition of HDAC1	B	8	pIC50	9.9	nM	IC50	Bioorg Med Chem (2010) 18: 3925-3933 [PMID:20452226]
ChEMBL	Inhibition of full length recombinant C-terminal FLAG/His-tagged human HDAC1 expressed in baculovirus infected Sf21 insect cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence plate reader assay	B	8.02	pIC50	9.59	nM	IC50	Bioorg Med Chem (2021) 50: 116454-116454 [PMID:34634618]
ChEMBL	Inhibition of HDAC1 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	8.04	pIC50	9.2	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant full length C-terminal His/FLAG tagged human HDAC1 (1 to 482 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition and further incubated for 2 hrs by fluorescence assay	B	8.05	pIC50	9	nM	IC50	J Med Chem (2019) 62: 8557-8577 [PMID:31414801]
ChEMBL	Inhibition of full length recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in baculovirus infected Sf21 insect cells using fluorogenic peptide p53 residues 379-382 (RHKK(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	8.05	pIC50	9	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of recombinant human HDAC1 by fluorimetry	B	8.07	pIC50	8.5	nM	IC50	J Med Chem (2016) 59: 655-670 [PMID:26705137]
ChEMBL	Inhibition of HDAC1 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	8.08	pIC50	8.41	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	8.09	pIC50	8.22	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human HDAC1 using RHKKAc as substrate by fluorescence assay	B	8.11	pIC50	7.7	nM	IC50	ACS Med Chem Lett (2017) 8: 510-515 [PMID:28523102]
ChEMBL	Inhibition of HDAC1 (unknown origin) after 15 mins by fluorescence assay	B	8.13	pIC50	7.4	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5497-5501 [PMID:25455492]
ChEMBL	Inhibition of human HDAC1 by fluorimetric assay	B	8.15	pIC50	7.12	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC1 using fluorogenic tetrapeptide RHKKAc as substrate	B	8.15	pIC50	7.12	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC1	B	8.15	pIC50	7	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of recombinant human C-terminal FLAG-His-tagged HDAC1 (1 to 482 residues) expressed in Sf21 cells using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorimetry analysis	B	8.16	pIC50	6.96	nM	IC50	Eur J Med Chem (2020) 190: 112086-112086 [PMID:32058238]
ChEMBL	Inhibition of human HDAC1 using RHKK(Ac) as substrate	B	8.16	pIC50	6.9	nM	IC50	Eur J Med Chem (2018) 150: 667-677 [PMID:29567459]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus infected insect cells using [RHKK(Ac)] fluorogenic substrate	B	8.16	pIC50	6.89	nM	IC50	Eur J Med Chem (2021) 217: 113338-113338 [PMID:33744690]
ChEMBL	Inhibition of recombinant human HDAC1 using fluorogenic substrate by fluorescence assay	B	8.16	pIC50	6.89	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	8.17	pIC50	6.7	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of full length human recombinant C-terminal FLAG-His-tagged HDAC1 (1 to 482 residues) expressed in Sf21 cells using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	8.18	pIC50	6.57	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of human HDAC1	B	8.2	pIC50	6.3	nM	IC50	Eur J Med Chem (2023) 248: 115054-115054 [PMID:36630883]
ChEMBL	Inhibition of human HDAC1 using Arg-His-Lys-Lys (Ac) as substrate incubated for 2 hrs by fluorescence assay	B	8.21	pIC50	6.22	nM	IC50	Eur J Med Chem (2019) 184: 111755-111755 [PMID:31627059]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	8.22	pIC50	6	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of HADC1 (unknown origin)	B	8.22	pIC50	6	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of HDAC1 in human NIH3T3 cells by radiometric histone deacetylation assay	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3651-3656 [PMID:19457659]
ChEMBL	Inhibition of human HDAC1	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127023-127023 [PMID:32067866]
ChEMBL	Inhibition of HDAC1	B	8.22	pIC50	6	nM	IC50	Bioorg Med Chem Lett (2012) 22: 1926-1930 [PMID:22321215]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate incubated for 30 mins by fluorescence based assay	B	8.25	pIC50	5.6	nM	IC50	ACS Med Chem Lett (2020) 11: 713-719 [PMID:32435375]
ChEMBL	Inhibition against partially purified human histone deacetylase 1 (HDAC-1)	B	8.3	pIC50	5	nM	IC50	J Med Chem (2002) 45: 2877-2885 [PMID:12061890]
ChEMBL	In vitro inhibition of partially purified recombinant human Histone deacetylase 1	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2001) 11: 2847-2850 [PMID:11597413]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	8.3	pIC50	5	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibitory concentration against recombinant human Histone deacetylase 1	B	8.3	pIC50	5	nM	IC50	J Med Chem (2003) 46: 5097-5116 [PMID:14613312]
ChEMBL	Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	B	8.3	pIC50	5	nM	IC50	J Med Chem (2012) 55: 9891-9899 [PMID:23009203]
ChEMBL	Inhibition of human recombinant HDAC1 using p53 residues 379-382 (RHKKAc) as substrate	B	8.31	pIC50	4.9	nM	IC50	Bioorg Med Chem (2013) 21: 5339-5354 [PMID:23820574]
ChEMBL	Inhibition of HDAC1 (unknown origin) after 30 mins by fluorometric analysis	B	8.37	pIC50	4.3	nM	IC50	Bioorg Med Chem (2013) 21: 4201-4209 [PMID:23719282]
ChEMBL	Inhibition of HDAC1	B	8.4	pIC50	4	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC1 after 17 hrs	B	8.4	pIC50	4	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of HDAC1 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	8.4	pIC50	4	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of human recombinant HDAC1 using fluorogenic substrate measured after 30 mins	B	8.45	pIC50	3.51	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Inhibition of human recombinant HDAC1 after 30 mins by fluorimetric assay	B	8.48	pIC50	3.3	nM	IC50	J Med Chem (2009) 52: 7836-7846 [PMID:19705846]
ChEMBL	Inhibition of human HDAC1 using rhodamine as substrate after 1 hrs by fluorescence assay	B	8.52	pIC50	3	nM	IC50	Bioorg Med Chem (2011) 19: 4626-4634 [PMID:21723733]
ChEMBL	Inhibition of HDAC1 by in vitro deacetylation assay	B	8.52	pIC50	3	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of human HDAC1 in presence of dithiothreitol by HDAC fluorescent assay	B	8.64	pIC50	2.3	nM	IC50	Eur J Med Chem (2012) 52: 111-122 [PMID:22465091]
ChEMBL	Inhibition of HDAC1 (unknown origin) by colorimetric method	B	8.66	pIC50	2.2	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of recombinant full-length human HDAC1 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	8.67	pIC50	2.14	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC1 (unknown origin) incubated for 1 hr by fluor de lys substrate based fluorescence method	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem (2015) 23: 6785-6793 [PMID:26481659]
ChEMBL	Inhibition of human HDAC1 expressed in baculovirus expression system in Sf9 cells using RHKKAc (379 to 382 residues) fluorogenic peptide as substrate and measured after 2 hrs by fluorescence based analysis	B	8.7	pIC50	2	nM	IC50	J Med Chem (2023) 66: 10528-10557 [PMID:37463500]
ChEMBL	Inhibition of human HDAC1 expressed in 293T cells	B	8.72	pIC50	1.9	nM	IC50	Bioorg Med Chem (2007) 15: 7830-7839 [PMID:17881232]
ChEMBL	Inhibition of HDAC1 (unknown origin)	B	8.8	pIC50	1.6	nM	IC50	Eur J Med Chem (2023) 254: 115327-115327 [PMID:37098307]
ChEMBL	Inhibition of full length recombinant human HDAC1 expressed in baculovirus infected Sf9 insect cells using RHKKAc fluorogenic peptide as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence assay	B	8.82	pIC50	1.5	nM	IC50	J Med Chem (2021) 64: 4810-4840 [PMID:33830764]
ChEMBL	Inhibition of recombinant full-length human HDAC1 using FITC-H3K27(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	9	pIC50	1	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of recombinant full length human HDAC1 expressed in baculovirus infected Sf9 cells using FAM-RHKK-Ac as substrate incubated for 17 hrs by electrophoretic mobility shift assay	B	9.17	pIC50	0.68	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of HDAC1	B	9.82	pIC50	0.15	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
ChEMBL	Inhibition of Histone deacetylase 1 induced acetylated histone in mammalian cells.	B	5.22	pEC50	6000	nM	EC50	J Med Chem (2003) 46: 4826-4829 [PMID:14584932]
ChEMBL	Concentration of compound required for acetylation of histone-4 in human T24 cancer cells	F	6	pEC50	1000	nM	EC50	Bioorg Med Chem Lett (2001) 11: 2847-2850 [PMID:11597413]
histone deacetylase 1/Histone deacetylase 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4001] [GtoPdb: 2658] [UniProtKB: O09106]
ChEMBL	Binding affinity towards HDAC1 (Histone deacetylase 1) in mouse A20 cells, expressed as binding constant (pKi)	B	8.97	pKi	1.07	nM	Ki	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Inhibitory activity against histone deacetylases (HDAC1) prepared from mouse melanoma B16/BL6 cells	B	7.72	pIC50	19	nM	IC50	Bioorg Med Chem Lett (2004) 14: 2427-2431 [PMID:15109626]
ChEMBL	In vitro for anti-HDAC1 (Histone deacetylase 1) activity in mouse A20 cells	B	8.7	pIC50	2	nM	IC50	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Inhibition of mouse Histone deacetylase 1 (HDAC1) receptor	B	8.7	pIC50	2	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	Inhibition of mouse HDAC1	B	8.7	pIC50	2	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	Inhibition of mouse HDAC1	B	8.74	pIC50	1.8	nM	IC50	J Med Chem (2006) 49: 6046-6056 [PMID:17004718]
ChEMBL	Inhibition of mouse liver HDAC1	B	8.74	pIC50	1.8	nM	IC50	J Med Chem (2008) 51: 1505-1529 [PMID:18247554]
histone deacetylase 10/Histone deacetylase 10 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5103] [GtoPdb: 2614] [UniProtKB: Q969S8]
ChEMBL	Inhibition of HDAC10	B	8.15	pKi	7	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
ChEMBL	Inhibition of human HDAC10 using RHKKAc as substrate by fluorescence assay	B	7.15	pIC50	71	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibition of HDAC10 (unknown origin) using fluorogenic peptide p53 residues 379-382 (RHKK(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	7.21	pIC50	61	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of recombinant human HDAC10 using fluorogenic substrate by fluorescence assay	B	7.42	pIC50	38.1	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of recombinant full-length human HDAC10 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	7.42	pIC50	37.9	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human recombinant HDAC10 using RHK-K(Ac)-AMC as substrate	B	7.46	pIC50	34.81	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of human HDAC10 using RHKKAc peptide as substrate	B	7.46	pIC50	34.5	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of HDAC10 (unknown origin) using RHKK(Ac)AMC as substrate after 1 to 2 hrs by fluorescence assay	B	7.49	pIC50	32	nM	IC50	Eur J Med Chem (2018) 143: 2005-2014 [PMID:29150335]
ChEMBL	Inhibition of HDAC10 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	7.51	pIC50	31	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	7.64	pIC50	22.8	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of human HDAC10 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.7	pIC50	20.1	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC10 by fluorimetric assay	B	7.7	pIC50	20.1	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC10	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of human recombinant HDAC10 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7.95	pIC50	11.3	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human recombinant HDAC10 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	8.05	pIC50	9	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of HDAC10 by in vitro deacetylation assay	B	8.1	pIC50	8	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of HDAC10	B	8.3	pIC50	5	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC10 after 17 hrs	B	8.3	pIC50	5	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of HDAC10 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	8.3	pIC50	5	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of recombinant full length human HDAC10 expressed in baculovirus infected Sf9 cells using FAM-RHKK-Ac as substrate incubated for 17 hrs by electrophoretic mobility shift assay	B	8.79	pIC50	1.61	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of recombinant full-length human HDAC10 using FITC-H3K27(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	9	pIC50	1	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
histone deacetylase 11/Histone deacetylase 11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3310] [GtoPdb: 2615] [UniProtKB: Q96DB2]
ChEMBL	Inhibition of human full length HDAC11 expressed in baculovirus infected Sf9 cells using fluorogenic HDAC class2a as substrate measured after 30 mins by fluorescence based assay	B	4.96	pIC50	10900	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
ChEMBL	Inhibition of recombinant full-length human HDAC11 using FAM-RHKK (trifluor-Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	5.04	pIC50	9080	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of HDAC11 (unknown origin) using fluorogenic peptide 382 RHKK(Ac)AM as substrate and measured by fluorescence assay	B	5.17	pIC50	6830	nM	IC50	Eur J Med Chem (2022) 228: 113971-113971 [PMID:34772529]
ChEMBL	Inhibition of human recombinant N-terminal Streptavidin2-tagged HDAC11 (1 to 347 residues) expressed in baculovirus infected insect cells using fluorogenic HDAC class 2a as substrate measured after 1 to 2 hrs by fluorescence assay	B	5.46	pIC50	3470	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of recombinant human HDAC11 using fluorogenic substrate by fluorescence assay	B	5.68	pIC50	2110	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of human HDAC11 by fluorescence assay	B	7.51	pIC50	31	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of HDAC11 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	7.6	pIC50	25	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of human recombinant HDAC11 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	7.76	pIC50	17.3	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human HDAC11 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.82	pIC50	15.2	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC11 by fluorimetric assay	B	7.82	pIC50	15.15	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC11	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of recombinant C-terminal His-tagged human HDAC11 (1 to 347 residues) expressed in Baculovirus infected insect cells using RHKK-Ac as substrate by fluorimetric assay	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2019) 162: 321-333 [PMID:30448419]
ChEMBL	Inhibition of human recombinant HDAC11 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7.86	pIC50	13.9	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human recombinant N-terminal His-tagged HDAC11 (1 to 347 residues) expressed in insect cells/baculovirus expression system using RHK-K(Ac)-AMC as substrate by fluorescence assay	B	7.94	pIC50	11.37	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of recombinant full-length human HDAC11 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	7.97	pIC50	10.6	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
histone deacetylase 2/Histone deacetylase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1937] [GtoPdb: 2616] [UniProtKB: Q92769]
ChEMBL	Binding affinity to C-terminal His-tagged human HDAC2 (2 to 488 residues) in transfected in baculovirus infected Sf9 cells assessed as dissociation constant	B	7.92	pKd	12	nM	Kd	J Med Chem (2023) 66: 11672-11700 [PMID:37651268]
ChEMBL	Inhibition of human recombinant HDAC2 by Michaelis-Menten equation analysis	B	8.97	pKi	1.06	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
ChEMBL	Inhibition of recombinant HDAC2	B	9.12	pKi	0.76	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
ChEMBL	Inhibition of human HDAC2	B	9.15	pKi	0.7	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of human HDAC2	B	9.19	pKi	0.65	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
GtoPdb	-	-	9.19	pKi	0.65	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of full length recombinant human HDAC2 expressed in baculovirus expression system using fluorogenic peptide RHKK(Ac) as substrate by fluorescence assay	B	4.59	pIC50	25800	nM	IC50	Bioorg Med Chem (2016) 24: 5183-5196 [PMID:27614919]
ChEMBL	Inhibition of HDAC2 by fluorescence assay	B	5.12	pIC50	7600	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of human full-length recombinant HDAC2 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.02	pIC50	96	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of human recombinant HDAC2 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7.17	pIC50	68	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of recombinant human HDAC2 by fluorimetry	B	7.28	pIC50	52	nM	IC50	J Med Chem (2016) 59: 655-670 [PMID:26705137]
ChEMBL	Inhibition of human HDAC2 using RHKKAc peptide as substrate	B	7.33	pIC50	46.6	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of recombinant human C-terminal GST-tagged HDAC2 (1 to 488 residues) expressed in baculovirus infected sf21 cells using RHK-K(Ac)-AMC as substrate after 60 mins by fluorimetry assay	B	7.4	pIC50	39.5	nM	IC50	Eur J Med Chem (2020) 187: 111915-111915 [PMID:31838329]
ChEMBL	Inhibition of human HDAC2 using RHKKAc as substrate by fluorescence assay	B	7.41	pIC50	39.1	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibitory concentration against histone deacetylase 2	B	7.41	pIC50	39	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4427-4431 [PMID:16137884]
ChEMBL	Inhibition of human HDAC2 by fluorescence assay	B	7.49	pIC50	32.1	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of full length recombinant C-terminal GST-fusion-tagged human HDAC2 expressed in insect cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence plate reader assay	B	7.54	pIC50	28.6	nM	IC50	Bioorg Med Chem (2021) 50: 116454-116454 [PMID:34634618]
ChEMBL	Inhibition of HDAC2 (unknown origin) after 15 mins by fluorescence assay	B	7.55	pIC50	28.2	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5497-5501 [PMID:25455492]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	7.6	pIC50	25	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant human GST-tagged HDAC2 (1 to 488 residues) expressed in baculovirus infected sf21 cells using fluorogenic peptide p53 residues 379-382 (RHKK(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	7.6	pIC50	25	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of HDAC2 (unknown origin) assessed as inhibition of fluorogenic peptide deacetylation	B	7.62	pIC50	24	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5222-5228 [PMID:27717544]
ChEMBL	Inhibition of HDAC2 (unknown origin) using RHKK(Ac)AMC as substrate after 1 to 2 hrs by fluorescence assay	B	7.63	pIC50	23.6	nM	IC50	Eur J Med Chem (2018) 143: 2005-2014 [PMID:29150335]
ChEMBL	Inhibition of human HDAC2 by ELISA	B	7.64	pIC50	23	nM	IC50	Eur J Med Chem (2018) 148: 73-85 [PMID:29454918]
ChEMBL	Inhibition of human HDAC2	B	7.64	pIC50	23	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of human HDAC2 by fluorimetric assay	B	7.64	pIC50	22.95	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC2	B	7.67	pIC50	21.5	nM	IC50	Eur J Med Chem (2023) 248: 115054-115054 [PMID:36630883]
ChEMBL	Inhibition of recombinant human HDAC2 using fluorogenic substrate by fluorescence assay	B	7.69	pIC50	20.5	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of recombinant human C-terminal GST-tagged HDAC2 (1 to 488 residues) expressed in insect cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	7.73	pIC50	18.7	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of HDAC2	B	7.74	pIC50	18	nM	IC50	Bioorg Med Chem Lett (2012) 22: 1926-1930 [PMID:22321215]
ChEMBL	Inhibition of recombinant full-length human HDAC2 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	7.83	pIC50	14.8	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of HDAC2 (unknown origin) after 30 mins by fluorometric analysis	B	7.85	pIC50	14	nM	IC50	Bioorg Med Chem (2013) 21: 4201-4209 [PMID:23719282]
ChEMBL	Inhibition of HDAC2 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	7.85	pIC50	14	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of HDAC2	B	7.85	pIC50	14	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC2 after 17 hrs	B	7.85	pIC50	14	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of full length human recombinant C-terminal GST-tagged HDAC2 (1 to 488 residues) expressed in insect cells using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	7.98	pIC50	10.51	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of human recombinant histone deacetylase 2	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2009) 19: 688-692 [PMID:19111466]
ChEMBL	Inhibition of HDAC2 by in vitro deacetylation assay	B	8	pIC50	10	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of HDAC2 (unknown origin) by colorimetric method	B	8.22	pIC50	6.08	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of human recombinant HDAC2 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	8.3	pIC50	5	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of human HDAC2 using rhodamine as substrate after 1 hrs by fluorescence assay	B	8.4	pIC50	4	nM	IC50	Bioorg Med Chem (2011) 19: 4626-4634 [PMID:21723733]
ChEMBL	Inhibition of recombinant full-length human HDAC2 using FITC-H3K27(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	8.52	pIC50	3	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of recombinant full length human HDAC2 expressed in baculovirus infected Sf9 cells using FAM-RHKK-Ac as substrate incubated for 17 hrs by electrophoretic mobility shift assay	B	8.56	pIC50	2.74	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of HDAC2 in human HeLa cell nuclear extracts in presence of dithiothreitol by HDAC fluorescent assay	B	8.96	pIC50	1.1	nM	IC50	Eur J Med Chem (2012) 52: 111-122 [PMID:22465091]
ChEMBL	Inhibition of HDAC2	B	9	pIC50	1	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
histone deacetylase 3/Histone deacetylase 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1829] [GtoPdb: 2617] [UniProtKB: O15379]
ChEMBL	Inhibition of recombinant HDAC3	B	9.24	pKi	0.58	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
GtoPdb	-	-	9.3	pKi	0.5	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC3	B	9.3	pKi	0.5	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC3	B	9.3	pKi	0.5	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of HDAC3 by fluorescence assay	B	5.28	pIC50	5200	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of human HDAC3 using RHKKAc peptide as substrate	B	7.28	pIC50	52.5	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of human recombinant HDAC3 using [RHKK(Ac)] fluorogenic substrate	B	7.43	pIC50	37.5	nM	IC50	Eur J Med Chem (2021) 217: 113338-113338 [PMID:33744690]
ChEMBL	Inhibition of recombinant human HDAC3 using fluorogenic substrate by fluorescence assay	B	7.43	pIC50	37.5	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of HDAC3 (unknown origin) by ELISA	B	7.51	pIC50	31	nM	IC50	Eur J Med Chem (2018) 148: 73-85 [PMID:29454918]
ChEMBL	Inhibition of human full-length recombinant HDAC3 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of human HDAC3 using RHKKAc as substrate by fluorescence assay	B	7.58	pIC50	26.2	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibition of HDAC3 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	7.68	pIC50	21	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of human KDAC3 using [3H]acetyl histone H4 peptide substrate incubated for 60 mins by scintillation counting method	B	7.7	pIC50	20	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of recombinant full length human C-terminal His-tagged HDAC3 (1 to 428 residues) expressed in baculovirus infected insect cells using fluorogenic peptide p53 residues 379-382 (RHKK(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	7.85	pIC50	14	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of HDAC3 (unknown origin) assessed as inhibition of fluorogenic peptide deacetylation	B	7.85	pIC50	14	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5222-5228 [PMID:27717544]
ChEMBL	Inhibition of human recombinant HDAC3 after 30 mins by fluorimetric assay	B	7.96	pIC50	11	nM	IC50	J Med Chem (2009) 52: 7836-7846 [PMID:19705846]
ChEMBL	Inhibition of recombinant human HDAC3 using RHK-K(Ac)-AMC as substrate by fluorescence based assay	B	7.98	pIC50	10.4	nM	IC50	Eur J Med Chem (2020) 190: 112086-112086 [PMID:32058238]
ChEMBL	Inhibition of human HDAC3 using RHKK(Ac) as substrate	B	7.98	pIC50	10.4	nM	IC50	Eur J Med Chem (2018) 150: 667-677 [PMID:29567459]
ChEMBL	Inhibition of human HDAC3 by fluorimetric assay	B	7.99	pIC50	10.32	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC3 complexed with NCOR2 using fluorogenic tetrapeptide RHKKAc as susbtrate	B	7.99	pIC50	10.3	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC3	B	8	pIC50	10	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	8.02	pIC50	9.6	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of HDAC3 by in vitro deacetylation assay	B	8.3	pIC50	5	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of HDAC3 (unknown origin) by colorimetric method	B	8.4	pIC50	4.01	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of recombinant full-length human HDAC3 using FAM-RHKK(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	8.52	pIC50	3	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of HDAC3 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	8.7	pIC50	2	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of HDAC3 after 17 hrs	B	8.7	pIC50	2	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of HDAC3	B	8.7	pIC50	2	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC3	B	9.22	pIC50	0.6	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
histone deacetylase 3/Histone deacetylase 3/NCoR1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038484] [GtoPdb: 2617] [UniProtKB: O15379, O75376]
ChEMBL	Inhibition of recombinant human HDAC3/NCOR1 complex expressed in insect cells assessed as residual enzyme activity preincubated for 1 hr followed by addition of fluorogenic Ac-LGK-ac-AMC as substrate for 30 mins by fluorescence analysis	B	9.27	pKi	0.54	nM	Ki	Medchemcomm (2016) 7: 464-470
GtoPdb	-	-	9.3	pKi	0.5	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
histone deacetylase 3/Histone deacetylase 3/Nuclear receptor corepressor 2 (HDAC3/NCoR2) in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111363] [GtoPdb: 2617] [UniProtKB: O15379, Q9Y618]
ChEMBL	Inhibition of full length recombinant human C-terminal His-tagged HDAC3/N-terminal GST-tagged recombinant human NCOR2 (395 to 489 residues) expressed in baculovirus-infected insect cells using RHKK(Ac)AMC as substrate after 60 mins by fluorimeter	B	9.08	pKi	0.84	nM	Ki	J Med Chem (2018) 61: 3454-3477 [PMID:29589441]
GtoPdb	-	-	9.3	pKi	0.5	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Displacement of fluorescent 5-(3-(3-(4-((4-((7-(hydroxyamino)-7-oxoheptyl)carbamoyl)phenylamino)methyl)-1H-1,2,3-triazol-1-yl)propyl)thioureido)-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)benzoic acid from human HDAC3/NcoR2 by fluorescence polarization assay	B	9.59	pKi	0.26	nM	Ki	Bioorg Med Chem Lett (2008) 18: 2809-2812 [PMID:18430569]
ChEMBL	Inhibition of human recombinant full length C-terminal His-tagged HDAC3 (1 to 428 residues)/N-terminaL GST-tagged human recombinant NCOR2 (395 to 489 residues) expressed in baculovirus infected insect cells using RHK-K(Ac)-AMC as substrate after 60 mins by fluorimetry analysis	B	7.6	pIC50	25.3	nM	IC50	Eur J Med Chem (2020) 187: 111915-111915 [PMID:31838329]
ChEMBL	Inhibition of recombinant full-length human HDAC3/NCOR2 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	7.8	pIC50	15.7	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human recombinant full length C-terminal His-tagged HDAC3 (1 to 428 residues)/N-terminal GST-tagged NCOR2 (395 to 489 residues) expressed in insect cells using RHK-K(Ac)-AMC as substrate by fluorimetric assay	B	7.89	pIC50	13	nM	IC50	Eur J Med Chem (2019) 162: 321-333 [PMID:30448419]
ChEMBL	Inhibition of full length human recombinant C-terminal His-tagged HDAC3 (1 to 428 residues)/human recombinant N-terminal GST-tagged NCOR2 expressed in insect cells using RHK-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	7.96	pIC50	11.07	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of C-terminal His-tagged human HDAC3 (1 to 428 residues)/N-terminal GST tagged human NCOR2 (395 to 489) expressed in baculovirus infected Sf9 insect cells using fluorogenic HDAC substrate measured after 30 mins by fluorimetry	B	8	pIC50	<10	nM	IC50	Bioorg Med Chem (2022) 56: 116599-116599 [PMID:35041998]
ChEMBL	Displacement of fluorescent 5-(3-(3-(4-((4-((7-(hydroxyamino)-7-oxoheptyl)carbamoyl)phenylamino)methyl)-1H-1,2,3-triazol-1-yl)propyl)thioureido)-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)benzoic acid from human HDAC3/NcoR2 by fluorescence polarization assay	B	8.05	pIC50	9	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2809-2812 [PMID:18430569]
histone deacetylase 4/Histone deacetylase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3524] [GtoPdb: 2659] [UniProtKB: P56524]
ChEMBL	Inhibition of human recombinant HDAC4 by Michaelis-Menten equation analysis	B	4.84	pKi	14547	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
GtoPdb	-	-	5.85	pKi	1400	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC4	B	5.85	pKi	1400	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of HDAC4 (unknown origin)	B	5.85	pKi	1400	nM	Ki	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of N-terminal GST-tagged recombinant human HDAC4 assessed as hydrolase activity using fluorogenic Ac-AC-LGK-tfa-AMC-AMC as substrate measured after 60 mins by fluorescence analysis	B	6.01	pKi	980	nM	Ki	Medchemcomm (2016) 7: 464-470
ChEMBL	Inhibition of human HDAC4	B	6.4	pKi	400	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of HDAC4 (unknown origin) using Boc-Lys(trifluoroacetyl)-AMC fluorogenic acetylated peptide substrate by fluorometric assay	B	5.04	pIC50	9100	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of human HDAC4 by fluorescence assay	B	5.07	pIC50	8439	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of HDAC4 by fluorescence assay	B	5.11	pIC50	7690	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of recombinant full-length human HDAC4 using Boc-Lys(trifluoroacetyl)-AMC as substrate measured after 2 hrs by fluorescence assay	B	5.18	pIC50	6600	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant human HDAC4 using Boc-Lys(trifluoroacetyl)-AMC as substrate by homogeneous fluorescence release assay	B	5.42	pIC50	3800	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
ChEMBL	Inhibition of recombinant full-length human HDAC4 using FAM-RHKK (trifluor-Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	5.92	pIC50	1210	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of recombinant full length human HDAC4 expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 1.5 hrs by electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of HDAC4 expressed in 293T cells	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem (2008) 16: 437-445 [PMID:17900911]
ChEMBL	Inhibition of human recombinant HDAC4 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	6.91	pIC50	122	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human recombinant HDAC4 using p53 residues 379-382 (RHKKAc) as substrate	B	7.07	pIC50	85	nM	IC50	Bioorg Med Chem (2013) 21: 5339-5354 [PMID:23820574]
ChEMBL	Inhibition of human HDAC4 expressed in 293T cells	B	7.2	pIC50	62.9	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2982-2984 [PMID:18397826]
ChEMBL	Inhibition of HDAC4	B	7.36	pIC50	44	nM	IC50	Bioorg Med Chem Lett (2010) 20: 997-999 [PMID:20045316]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	7.46	pIC50	34.8	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of HDAC4 by fluorimetric assay	B	7.47	pIC50	34	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6770-6772 [PMID:23021104]
ChEMBL	Inhibition of HDAC4	B	7.47	pIC50	34	nM	IC50	J Med Chem (2006) 49: 4809-4812 [PMID:16884291]
ChEMBL	Inhibition of human recombinant HDAC4 expressed in 293 cells	B	7.47	pIC50	34	nM	IC50	J Med Chem (2007) 50: 5425-5438 [PMID:17929798]
ChEMBL	Inhibition of human HDAC4 using fluorescent substrate Ac-KGLGK(Ac)-MCA after 30 mins by fluorescence plate reader	B	7.47	pIC50	34	nM	IC50	Bioorg Med Chem (2014) 22: 3850-3855 [PMID:24997578]
ChEMBL	Inhibition of recombinant human HDAC4 expressed in HEK293T cells using Ac-KGLGK(Ac)-MCA as substrate measured after 30 mins by fluorescence assay	B	7.47	pIC50	34	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human HDAC4	B	7.54	pIC50	29	nM	IC50	Bioorg Med Chem Lett (2007) 17: 4895-4900 [PMID:17588744]
ChEMBL	Inhibition of human histone deacetylase 4 prepared from 293T cells	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2004) 14: 5343-5346 [PMID:15454224]
ChEMBL	Inhibition of HDAC4 in human HeLa cells incubated for 1 hr by mass spectrometry method	B	7.82	pIC50	15	nM	IC50	Bioorg Med Chem (2021) 52: 116510-116510 [PMID:34826681]
ChEMBL	Inhibition of human HDAC4 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.92	pIC50	12.1	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC4 by fluorimetric assay	B	7.92	pIC50	12.07	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC4	B	7.92	pIC50	12	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of HDAC4 by in vitro deacetylation assay	B	8.1	pIC50	8	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of human HDAC4 expressed in 293T cells	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem (2007) 15: 7830-7839 [PMID:17881232]
ChEMBL	Inhibition of acetylation of histone-4 in human T-24 cancer cells	F	5.4	pEC50	4000	nM	EC50	J Med Chem (2003) 46: 5097-5116 [PMID:14613312]
ChEMBL	Inhibition of acetylation of histone-4 in human T-24 cancer cells	F	6	pEC50	1000	nM	EC50	J Med Chem (2003) 46: 5097-5116 [PMID:14613312]
histone deacetylase 5/Histone deacetylase 5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2563] [GtoPdb: 2660] [UniProtKB: Q9UQL6]
ChEMBL	Inhibition of human recombinant HDAC5 by Michaelis-Menten equation analysis	B	5.39	pKi	4120	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
ChEMBL	Inhibition of human HDAC5	B	6.59	pKi	260	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC5	B	6.59	pKi	260	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
GtoPdb	-	-	6.59	pKi	260	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC5 by fluorescence assay	B	5.21	pIC50	6155	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of HDAC5 (unknown origin) using Boc-Lys(trifluoroacetyl)-AMC fluorogenic acetylated peptide substrate by fluorometric assay	B	5.85	pIC50	1400	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant full-length human HDAC5 using FAM-RHKK (trifluor-Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	5.86	pIC50	1380	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of recombinant full-length human HDAC5 using Boc-Lys(trifluoroacetyl)-AMC as substrate measured after 2 hrs by fluorescence assay	B	5.96	pIC50	1090	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant full length human HDAC5 expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 3 hrs by electrophoretic mobility shift assay	B	6.11	pIC50	776	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of human HDAC5 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.78	pIC50	16.5	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC5 by fluorimetric assay	B	7.78	pIC50	16.48	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC5	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	7.9	pIC50	12.5	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of human recombinant HDAC5 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	8.09	pIC50	8.08	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of HDAC5 by in vitro deacetylation assay	B	8.22	pIC50	6	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
histone deacetylase 5/Histone deacetylase 5 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2768] [GtoPdb: 2660] [UniProtKB: Q9Z2V6]
ChEMBL	Inhibitory activity against histone deacetylase (HDAC4) prepared from mouse melanoma B16/BL6 cells	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2004) 14: 2427-2431 [PMID:15109626]
histone deacetylase 6/Histone deacetylase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7]
ChEMBL	Inhibition of human recombinant HDAC6 by Michaelis-Menten equation analysis	B	8.52	pKi	3.02	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
GtoPdb	-	-	9	pKi	1	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC6	B	9	pKi	1	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC6	B	9	pKi	1	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of recombinant HDAC6	B	9	pKi	0.99	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
ChEMBL	Inhibition of full length recombinant human N-terminal GST-tagged HDAC6 expressed in baculovirus-infected Sf9 insect cells using RHKK(Ac)AMC as substrate after 90 mins by fluorimeter	B	9.24	pKi	0.58	nM	Ki	J Med Chem (2018) 61: 3454-3477 [PMID:29589441]
ChEMBL	Displacement of fluorescent 5-(3-(3-(4-((4-((7-(hydroxyamino)-7-oxoheptyl)carbamoyl)phenylamino)methyl)-1H-1,2,3-triazol-1-yl)propyl)thioureido)-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)benzoic acid from human HDAC6 by fluorescence polarization assay	B	9.89	pKi	0.13	nM	Ki	Bioorg Med Chem Lett (2008) 18: 2809-2812 [PMID:18430569]
ChEMBL	Inhibition of human HDAC6 using fluorogenic-(RHKKAc) as substrate by fluorescence assay	B	5.44	pIC50	3600	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of human HDAC6 using RHKK(Ac) as substrate	B	5.59	pIC50	2600	nM	IC50	Eur J Med Chem (2018) 150: 667-677 [PMID:29567459]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	5.92	pIC50	1210	nM	IC50	Eur J Med Chem (2018) 150: 506-524 [PMID:29549837]
ChEMBL	Inhibition of HDAC6 by fluorescence assay	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of HDAC6 expressed in 293T cells	B	6.55	pIC50	280	nM	IC50	Bioorg Med Chem (2008) 16: 437-445 [PMID:17900911]
ChEMBL	Inhibitory concentration against histone deacetylase 6	B	7.04	pIC50	91	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4427-4431 [PMID:16137884]
ChEMBL	Inhibition of human HDAC6 expressed in 293T cells	B	7.08	pIC50	83.3	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2982-2984 [PMID:18397826]
ChEMBL	Inhibition of HDAC6	B	7.09	pIC50	81	nM	IC50	J Med Chem (2006) 49: 4809-4812 [PMID:16884291]
ChEMBL	Inhibition of HDAC6 by fluorimetric assay	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6770-6772 [PMID:23021104]
ChEMBL	Inhibition of HDAC6 (unknown origin) incubated for 1 hr by fluor de lys substrate based fluorescence method	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem (2015) 23: 6785-6793 [PMID:26481659]
ChEMBL	Inhibition of HDAC6	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem Lett (2010) 20: 997-999 [PMID:20045316]
ChEMBL	Inhibition of recombinant human HDAC6 using Boc-Lys(Ac)-AMC as substrate by fluorometric analysis	B	7.25	pIC50	56	nM	IC50	Bioorg Med Chem Lett (2010) 20: 7067-7070 [PMID:20947351]
ChEMBL	Inhibition of human HDAC6	B	7.28	pIC50	53	nM	IC50	Bioorg Med Chem Lett (2007) 17: 4895-4900 [PMID:17588744]
ChEMBL	Inhibition of human HDAC6 using rhodamine as substrate after 1 hrs by fluorescence assay	B	7.29	pIC50	51	nM	IC50	Bioorg Med Chem (2011) 19: 4626-4634 [PMID:21723733]
ChEMBL	Inhibition of human KDAC6 using [3H]acetyl histone H4 peptide substrate incubated for 60 mins by scintillation counting method	B	7.42	pIC50	38	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of human recombinant histone deacetylase 6	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2009) 19: 688-692 [PMID:19111466]
ChEMBL	Inhibition of human histone deacetylase 6 prepared from 293T cells	B	7.7	pIC50	20	nM	IC50	Bioorg Med Chem Lett (2004) 14: 5343-5346 [PMID:15454224]
ChEMBL	Inhibition of HDAC6 (unknown origin) measured by fluorometry assay	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2022) 240: 114573-114573 [PMID:35797900]
ChEMBL	Inhibition of full length recombinant human HDAC6 expressed in baculovirus infected Sf9 insect cells using RHKKAc fluorogenic peptide as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence assay	B	7.77	pIC50	16.8	nM	IC50	J Med Chem (2021) 64: 4810-4840 [PMID:33830764]
ChEMBL	Displacement of fluorescent 5-(3-(3-(4-((4-((7-(hydroxyamino)-7-oxoheptyl)carbamoyl)phenylamino)methyl)-1H-1,2,3-triazol-1-yl)propyl)thioureido)-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)benzoic acid from human HDAC6 by fluorescence polarization assay	B	7.77	pIC50	16.8	nM	IC50	Bioorg Med Chem Lett (2008) 18: 2809-2812 [PMID:18430569]
ChEMBL	Inhibition of human recombinant HDAC6 expressed in baculovirus infected insect cells using BATCP as substrate after 4 hrs by UHPLC-ESI-MS/MS analysis	B	7.8	pIC50	16	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of human recombinant HDAC6 after 30 mins using fluorogenic substrate by fluorimetric assay	B	7.82	pIC50	15	nM	IC50	J Med Chem (2018) 61: 6574-6591 [PMID:30004697]
ChEMBL	Inhibition of human full-length recombinant HDAC6 preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	7.82	pIC50	15	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of HDAC6	B	7.89	pIC50	13	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	7.95	pIC50	11.1	nM	IC50	Eur J Med Chem (2021) 226: 113874-113874 [PMID:34619465]
ChEMBL	Inhibition of human HDAC6 using fluorogenic HDAC substrate	B	8.03	pIC50	9.3	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	8.05	pIC50	9	nM	IC50	J Med Chem (2022) 65: 3080-3097 [PMID:35148101]
ChEMBL	Inhibition of recombinant human HDAC6 by fluorimetry	B	8.05	pIC50	9	nM	IC50	J Med Chem (2016) 59: 655-670 [PMID:26705137]
ChEMBL	Inhibition of human recombinant HDAC6 using fluorogenic HDAC substrate 3 pre-incubated for 30 mins followed by HDAC developer addition and measured after 15 mins by fluorogenic assay	B	8.05	pIC50	8.92	nM	IC50	Bioorg Med Chem (2019) 27: 3408-3420 [PMID:31235266]
ChEMBL	Inhibition of human recombinant HDAC6 using fluorogenic substrate measured after 30 mins	B	8.05	pIC50	8.92	nM	IC50	Bioorg Med Chem (2023) 79: 117154-117154 [PMID:36645952]
ChEMBL	Determination of IC50 values for inhibition of enzymatic assay of human HDAC6 with custom peptide substrate	B	8.06	pIC50	8.73	nM	IC50	HDAC6 screening dataset using tau-based substrate in an enzymatic assay yields selective inhibitors and activators
ChEMBL	Inhibition of HADC6 (unknown origin)	B	8.07	pIC50	8.6	nM	IC50	J Med Chem (2020) 63: 23-39 [PMID:31415174]
ChEMBL	Inhibition of N-terminal GST-tagged recombinant human HDAC6 expressed in baculovirus infected insect cells using RHKK(Ac)AMC as substrate by fluorescence-based assay	B	8.07	pIC50	8.5	nM	IC50	J Med Chem (2018) 61: 10299-10309 [PMID:30365892]
ChEMBL	Inhibition of HDAC6 (unknown origin) after 15 mins by fluorescence assay	B	8.09	pIC50	8.2	nM	IC50	Bioorg Med Chem Lett (2014) 24: 5497-5501 [PMID:25455492]
ChEMBL	Inhibition of HDAC6 in human SHSY5Y cells using BATCP as substrate after 8 hrs by UHPLC-ESI-MS/MS analysis	B	8.11	pIC50	7.8	nM	IC50	Bioorg Med Chem Lett (2016) 26: 4955-4959 [PMID:27650925]
ChEMBL	Inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in baculovirus infected insect cells using RHK-K(Ac)-AMC as substrate after 90 mins by fluorimetry analysis	B	8.21	pIC50	6.12	nM	IC50	Eur J Med Chem (2020) 187: 111915-111915 [PMID:31838329]
ChEMBL	Inhibition of human recombinant HDAC6 after 30 mins by fluorimetric assay	B	8.22	pIC50	6	nM	IC50	J Med Chem (2009) 52: 7836-7846 [PMID:19705846]
ChEMBL	Inhibition of recombinant N-terminal GST-tagged human HDAC6 (1 to 1215 residues) expressed in Baculovirus infected insect cells using RHKK-Ac as substrate by fluorimetric assay	B	8.23	pIC50	5.9	nM	IC50	Eur J Med Chem (2019) 162: 321-333 [PMID:30448419]
ChEMBL	Inhibition of HDAC6 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	8.25	pIC50	5.56	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human N-terminal GST-tagged HDAC6 expressed in baculovirus infected insect cells using RHKKAc as substrate	B	8.3	pIC50	5	nM	IC50	J Med Chem (2020) 63: 295-308 [PMID:31793776]
ChEMBL	Inhibition of human HDAC6 (unknown origin)	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2020) 30: 127023-127023 [PMID:32067866]
ChEMBL	Inhibition of HDAC6 (unknown origin) assessed as inhibition of fluorogenic peptide deacetylation	B	8.3	pIC50	5	nM	IC50	Bioorg Med Chem Lett (2016) 26: 5222-5228 [PMID:27717544]
ChEMBL	Inhibition of recombinant human HDAC6 using fluorogenic substrate by fluorescence assay	B	8.34	pIC50	4.53	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of human recombinant HDAC6 expressed in baculovirus infected insect cells using [RHKK(Ac)] fluorogenic substrate	B	8.34	pIC50	4.53	nM	IC50	Eur J Med Chem (2021) 217: 113338-113338 [PMID:33744690]
ChEMBL	Inhibition of full length recombinant human N-terminal GST-tagged HDAC6 expressed in baculovirus infected insect cells using RHK-K(Ac)-AMC as substrate after 90 mins by fluorescence assay	B	8.45	pIC50	3.55	nM	IC50	J Med Chem (2018) 61: 905-917 [PMID:29304284]
ChEMBL	Inhibition of human HDAC6 using RHKKAc as substrate by fluorescence assay	B	8.45	pIC50	3.55	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibition of human HDAC6 using RHKKAc peptide as substrate	B	8.46	pIC50	3.49	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of human recombinant HDAC6 using RHKKAcAMC as substrate by fluorescence assay	B	8.5	pIC50	3.13	nM	IC50	Eur J Med Chem (2018) 152: 329-357 [PMID:29738953]
ChEMBL	Inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1125 residues) expressed in baculovirus infected insect cells using fluorogenic peptide p53 residues 379-382 (RHKK(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	8.52	pIC50	3	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of recombinant human HDAC6 using RHK-K(Ac)-AMC as substrate by fluorescence based assay	B	8.59	pIC50	2.6	nM	IC50	Eur J Med Chem (2020) 190: 112086-112086 [PMID:32058238]
ChEMBL	Inhibition of human recombinant HDAC6 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	8.64	pIC50	2.31	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human HDAC6 using RHKKAc as substrate by fluorescence assay	B	8.64	pIC50	2.3	nM	IC50	ACS Med Chem Lett (2017) 8: 510-515 [PMID:28523102]
ChEMBL	Inhibition of HDAC6 (unknown origin)	B	8.66	pIC50	2.2	nM	IC50	Eur J Med Chem (2023) 254: 115327-115327 [PMID:37098307]
ChEMBL	Inhibition of full length recombinant N-terminal GST-tagged human HDAC6 expressed in baculovirus infected Sf9 insect cells using RHK-K(Ac)-AMC as substrate measured after 90 mins by fluorescence plate reader assay	B	8.68	pIC50	2.11	nM	IC50	Bioorg Med Chem (2021) 50: 116454-116454 [PMID:34634618]
ChEMBL	Inhibition of HDAC6 (unknown origin) using RHKK(Ac)AMC as substrate after 1 to 2 hrs by fluorescence assay	B	8.68	pIC50	2.09	nM	IC50	Eur J Med Chem (2018) 143: 2005-2014 [PMID:29150335]
ChEMBL	Inhibition of human HDAC6	B	8.69	pIC50	2.05	nM	IC50	Eur J Med Chem (2023) 248: 115054-115054 [PMID:36630883]
ChEMBL	Inhibition of recombinant human HDAC6 using RHK-K(Ac) as substrate by homogeneous fluorescence release assay	B	8.7	pIC50	2	nM	IC50	Eur J Med Chem (2019) 161: 277-291 [PMID:30366254]
ChEMBL	Inhibition of recombinant N-terminal GST-tagged human HDAC6 (1 to 1215 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition and further incubated for 2 hrs by fluorescence assay	B	8.7	pIC50	2	nM	IC50	J Med Chem (2019) 62: 8557-8577 [PMID:31414801]
ChEMBL	Inhibition of recombinant full-length human HDAC6 using FAM-RHKK(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	8.7	pIC50	2	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of human HDAC6 using Arg-His-Lys-Lys (Ac) as substrate incubated for 2 hrs by fluorescence assay	B	8.71	pIC50	1.94	nM	IC50	Eur J Med Chem (2019) 184: 111755-111755 [PMID:31627059]
ChEMBL	Inhibition of full length human recombinant N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in sf9 cells using RHK-K(Ac)-AMC as substrate incubated for 90 mins by fluorescence assay	B	8.77	pIC50	1.72	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of human recombinant HDAC6 using p53 residues 379-382 (RHKKAc) as substrate	B	8.8	pIC50	1.6	nM	IC50	Bioorg Med Chem (2013) 21: 5339-5354 [PMID:23820574]
ChEMBL	Inhibition of recombinant full-length human HDAC6 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	8.81	pIC50	1.56	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant human full-length N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in baculovirus infected Sf9 insect cells using RHKK(Ac)AMC as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence assay	B	8.82	pIC50	1.5	nM	IC50	Bioorg Med Chem (2021) 35: 116085-116085 [PMID:33668008]
ChEMBL	Inhibition of human HDAC6 by fluorescence assay	B	8.82	pIC50	1.5	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	8.85	pIC50	1.4	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	B	8.92	pIC50	1.2	nM	IC50	J Med Chem (2012) 55: 9891-9899 [PMID:23009203]
ChEMBL	Inhibition of HDAC6 (unknown origin) using RHKK(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	8.96	pIC50	1.1	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of HDAC6 (unknown origin) by colorimetric method	B	8.97	pIC50	1.07	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of HDAC6	B	9	pIC50	1	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of human HDAC6 expressed in baculovirus expression system in Sf9 cells using RHKKAc (379 to 382 residues) fluorogenic peptide as substrate and measured after 2 hrs by fluorescence based analysis	B	9	pIC50	1	nM	IC50	J Med Chem (2023) 66: 10528-10557 [PMID:37463500]
ChEMBL	Inhibition of HDAC6 after 17 hrs	B	9	pIC50	1	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
ChEMBL	Inhibition of HDAC6 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	9	pIC50	1	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of human recombinant HDAC6 expressed in baculovirus/sf9 cells using RHKKAc as substrate	B	9.05	pIC50	0.9	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of HDAC6 by in vitro deacetylation assay	B	9.1	pIC50	0.8	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of human HDAC6 by fluorimetric assay	B	9.38	pIC50	0.42	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC6 using fluorogenic tetrapeptide RHKKAc as substrate	B	9.38	pIC50	0.42	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC6	B	9.4	pIC50	0.4	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of HDAC6 in human HeLa cells assessed as inhibition of tubulin deacetylation incubated for overnight by cELISA	B	8.28	pEC50	5.2	nM	EC50	Bioorg Med Chem Lett (2016) 26: 154-159 [PMID:26611919]
histone deacetylase 6/Histone deacetylase 6 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2878] [GtoPdb: 2618] [UniProtKB: Q9Z2V5]
ChEMBL	Inhibition of mouse recombinant HDAC6 expressed in 293 cells	B	7.09	pIC50	81	nM	IC50	J Med Chem (2007) 50: 5425-5438 [PMID:17929798]
ChEMBL	Inhibition of mouse HDAC6 using fluorescent substrate Ac-KGLGK(Ac)-MCA after 30 mins by fluorescence plate reader	B	7.19	pIC50	65	nM	IC50	Bioorg Med Chem (2014) 22: 3850-3855 [PMID:24997578]
ChEMBL	Inhibition of recombinant mouse HDAC6 expressed in HEK293T cells using Ac-KGLGK(Ac)-MCA as substrate measured after 30 mins by fluorescence assay	B	7.19	pIC50	65	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibitory activity against histone deacetylase 6 prepared from mouse melanoma B16/BL6 cells	B	7.55	pIC50	28	nM	IC50	Bioorg Med Chem Lett (2004) 14: 2427-2431 [PMID:15109626]
ChEMBL	Inhibition of recombinant mouse HDAC6 expressed in HEK293T cells co-expressing mouse HDA2 using Ac-KGLGK(Ac)-MCA as substrate after 30 mins in presence of DTT by fluorescence assay	B	7.55	pIC50	28	nM	IC50	Bioorg Med Chem (2018) 26: 1920-1928 [PMID:29519604]
ChEMBL	Inhibition of mouse HDAC6 expressed in 293T cells	B	8.55	pIC50	2.8	nM	IC50	Bioorg Med Chem (2007) 15: 7830-7839 [PMID:17881232]
histone deacetylase 7/Histone deacetylase 7 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2716] [GtoPdb: 2661] [UniProtKB: Q8WUI4]
ChEMBL	Inhibition of N-terminal 6His-tagged recombinant human HDAC7 assessed as hydrolase activity using fluorogenic AC-LGK-tfa-AMC as substrate measured after 60 mins by fluorescence analysis	B	6.52	pKi	300	nM	Ki	Medchemcomm (2016) 7: 464-470
ChEMBL	Inhibition of human HDAC7	B	6.71	pKi	195	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC7	B	6.71	pKi	195	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
GtoPdb	-	-	6.71	pKi	195	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of recombinant full-length human HDAC7 using Boc-Lys(trifluoroacetyl)-AMC as substrate measured after 2 hrs by fluorescence assay	B	5.38	pIC50	4170	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of HDAC7 (unknown origin) using Boc-Lys(trifluoroacetyl)-AMC fluorogenic acetylated peptide substrate by fluorometric assay	B	5.64	pIC50	2300	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant full length human HDAC7 expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 3 hrs by electrophoretic mobility shift assay	B	6.32	pIC50	482	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	Inhibition of recombinant full-length human HDAC7 using FAM-RHKK (trifluor-Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	6.36	pIC50	440	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of HDAC7 by in vitro deacetylation assay	B	6.7	pIC50	200	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	6.71	pIC50	197	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of human recombinant HDAC7 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	6.86	pIC50	139	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of human recombinant HDAC7 using ArgHisLysLys(Ac) as substrate by fluorescence based analysis	B	7.05	pIC50	90	nM	IC50	J Med Chem (2023) 66: 7698-7729 [PMID:37276138]
ChEMBL	Inhibition of human HDAC7 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.65	pIC50	22.5	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC7 by fluorimetric assay	B	7.65	pIC50	22.46	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC7	B	7.66	pIC50	22	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
Histone deacetylase 8 in Schistosoma mansoni (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3797017] [UniProtKB: A5H660]
ChEMBL	Inhibition of Schistosoma mansoni KDAC8 using (FAM)-labeled peptide as substrate after 60 mins by microfluidic assay	B	5.44	pIC50	3620	nM	IC50	Bioorg Med Chem (2017) 25: 2105-2132 [PMID:28259528]
histone deacetylase 8/Histone deacetylase 8 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3192] [GtoPdb: 2619] [UniProtKB: Q9BY41]
ChEMBL	Inhibition of recombinant human HDAC8 expressed in Escherichia coli assessed as hydrolase activity using fluorogenic AC-LGK-tfa-AMC as substrate measured after 60 mins by fluorescence analysis	B	6.7	pKi	200	nM	Ki	Medchemcomm (2016) 7: 464-470
ChEMBL	Inhibition of recombinant HDAC8	B	6.89	pKi	130	nM	Ki	Eur J Med Chem (2010) 45: 2095-2116 [PMID:20223566]
ChEMBL	Inhibition of human recombinant HDAC8 by Michaelis-Menten equation analysis	B	7.16	pKi	69.65	nM	Ki	ACS Med Chem Lett (2013) 4: 757-761 [PMID:24900743]
ChEMBL	Activity of human HDAC8	B	7.35	pKi	45	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC8	B	7.35	pKi	45	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
ChEMBL	Inhibition of C-terminal His-tagged human recombinant full-length HDAC8 expressed in baculovirus expression system assessed as reduction in 7-amino-4-methylcoumarin by fluorescence based assay	B	7.35	pKi	45	nM	Ki	Eur J Med Chem (2017) 141: 188-196 [PMID:29031066]
GtoPdb	-	-	8.35	pKi	4.5	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of HDAC8 by fluorescence assay	B	4.14	pIC50	73100	nM	IC50	Bioorg Med Chem Lett (2011) 21: 1218-1221 [PMID:21256006]
ChEMBL	Inhibition of human KDAC8 using [3H]acetyl histone H4 peptide substrate incubated for 60 mins by scintillation counting method	B	5.34	pIC50	4550	nM	IC50	J Med Chem (2016) 59: 1613-1633 [PMID:26681404]
ChEMBL	Inhibition of HDAC8	B	5.86	pIC50	1380	nM	IC50	J Med Chem (2008) 51: 3437-3448 [PMID:18494463]
ChEMBL	Inhibition of HDAC8 assessed as blockade of decorboxylation of carboxyfluorescein labeled acetylated peptide substrate after 17 hrs	B	5.86	pIC50	1380	nM	IC50	J Med Chem (2010) 53: 1347-1356 [PMID:20055418]
ChEMBL	Inhibition of HDAC8 (unknown origin) after 30 mins by fluorometric analysis	B	5.87	pIC50	1360	nM	IC50	Bioorg Med Chem (2013) 21: 4201-4209 [PMID:23719282]
ChEMBL	Inhibition of HDAC8 (unknown origin) using Fluor de lys as substrate by fluorescence based analysis	B	5.94	pIC50	1160	nM	IC50	Eur J Med Chem (2020) 187: 111950-111950 [PMID:31865013]
ChEMBL	Inhibition of human HDAC8 using RHKAcKAc as substrate	B	5.99	pIC50	1020	nM	IC50	Eur J Med Chem (2018) 150: 667-677 [PMID:29567459]
ChEMBL	Inhibition of recombinant C-terminal His-tagged human HDAC8 (1 to 377 residues) expressed in Baculovirus infected insect cells using RHK-K(Ac)-AMC as substrate by fluorimetric assay	B	6	pIC50	990	nM	IC50	Eur J Med Chem (2019) 162: 321-333 [PMID:30448419]
ChEMBL	Inhibition of N terminal hexahistidine-tagged human HDAC8 expressed in Sf9 cells after 1 hr by fluorescence assay	B	6.02	pIC50	960	nM	IC50	Bioorg Med Chem (2011) 19: 4626-4634 [PMID:21723733]
ChEMBL	Inhibition of recombinant C-terminal His-tagged and N-terminal Streptavidin2-tagged human HDAC8 (1 to 377 residues) expressed in baculovirus infected insect cells using RHK-K(Ac)-AMC as substrate after 60 mins by fluorescence assay	B	6.03	pIC50	944	nM	IC50	Eur J Med Chem (2020) 187: 111915-111915 [PMID:31838329]
ChEMBL	Inhibition of human HDAC8	B	6.03	pIC50	923	nM	IC50	Eur J Med Chem (2023) 248: 115054-115054 [PMID:36630883]
ChEMBL	Inhibition of recombinant human HDAC8 using RHK-K(Ac)-AMC as substrate by fluorescence based assay	B	6.09	pIC50	820	nM	IC50	Eur J Med Chem (2020) 190: 112086-112086 [PMID:32058238]
ChEMBL	Inhibition of HDAC8 (unknown origin) measured by fluorometry assay	B	6.1	pIC50	790	nM	IC50	Eur J Med Chem (2022) 240: 114573-114573 [PMID:35797900]
ChEMBL	Inhibition of human recombinant histone deacetylase 8	B	6.11	pIC50	770	nM	IC50	Bioorg Med Chem Lett (2009) 19: 688-692 [PMID:19111466]
ChEMBL	Inhibition of recombinant C-terminal His-fusion tagged/N-terminal Strep2 tagged human HDAC8 (1 to 377 residues) expressed in insect cells using RHK-K(Ac)-K(Ac)-AMC as substrate measured after 60 mins by fluorescence plate reader assay	B	6.16	pIC50	697	nM	IC50	Bioorg Med Chem (2021) 50: 116454-116454 [PMID:34634618]
ChEMBL	Inhibition of recombinant human C-terminal His-tagged HDAC8 (1 to 377 residues) expressed in insect cells using RHK-K(Ac)-AMC as substrate measured after 60 mins by fluorescence assay	B	6.19	pIC50	650	nM	IC50	Bioorg Med Chem (2020) 28: 115250-115250 [PMID:31924504]
ChEMBL	Inhibition of C-terminal His-tagged recombinant human HDAC8 expressed in baculovirus infected insect cells using RHKK(Ac)AMC as substrate by fluorescence-based assay	B	6.22	pIC50	609	nM	IC50	J Med Chem (2018) 61: 10299-10309 [PMID:30365892]
ChEMBL	Inhibition of human HDAC8 expressed in baculovirus expression system in Sf9 cells using RHKAcKAc (379 to 382 residues) fluorogenic peptide as substrate and measured after 2 hrs by fluorescence based analysis	B	6.23	pIC50	589	nM	IC50	J Med Chem (2023) 66: 10528-10557 [PMID:37463500]
ChEMBL	Inhibition of human HDAC8 using RHKAcKAc peptide as substrate	B	6.25	pIC50	566	nM	IC50	J Med Chem (2018) 61: 7087-7102 [PMID:30028616]
ChEMBL	Inhibition of recombinant human full length C-terminal His-tagged HDAC8 expressed in baculovirus infected Sf9 cells using fluorogenic HDAC class2a as substrate measured after 30 mins by fluorescence based assay	B	6.31	pIC50	490	nM	IC50	J Med Chem (2018) 61: 1552-1575 [PMID:29360358]
ChEMBL	Inhibition of human HDAC8 using RHKAcKAc as substrate by fluorescence assay	B	6.37	pIC50	427	nM	IC50	Eur J Med Chem (2019) 162: 612-630 [PMID:30476825]
ChEMBL	Inhibition of human full-length recombinant C-terminal His-tagged HDAC8 expressed in baculovirus infected Sf9 insect cells preincubated for 5 mins followed by HDAC substrate addition and further incubated for 45 mins by fluorescence microplate reader assay	B	6.38	pIC50	420	nM	IC50	Eur J Med Chem (2021) 222: 113569-113569 [PMID:34111829]
ChEMBL	Inhibition of human recombinant HDAC8 using fluor de Lys as substrate preincubated for 5 mins followed by substrate addition measured after 25 mins by microplate reader analysis	B	6.41	pIC50	390	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6621-6627 [PMID:23010266]
ChEMBL	Inhibition of recombinant C-terminal His-fusion tagged and N-terminal Streptavidin2-tagged human HDAC8 (1 to 377 residues) expressed in insect cells using fluorogenic peptide p53 residues 379-382 (RHK(Ac)K(Ac)AMC) as substrate measured after 1 to 2 hrs by fluorescence assay	B	6.43	pIC50	371	nM	IC50	J Med Chem (2020) 63: 4256-4292 [PMID:32212730]
ChEMBL	Inhibition of recombinant human HDAC8 by fluorimetry	B	6.44	pIC50	360	nM	IC50	J Med Chem (2016) 59: 655-670 [PMID:26705137]
ChEMBL	Inhibition of recombinant human HDAC8 using fluorogenic substrate by fluorescence assay	B	6.5	pIC50	314	nM	IC50	Eur J Med Chem (2017) 125: 1268-1278 [PMID:27886544]
ChEMBL	Inhibition of human recombinant HDAC8 using [RHKK(Ac)] fluorogenic substrate	B	6.5	pIC50	314	nM	IC50	Eur J Med Chem (2021) 217: 113338-113338 [PMID:33744690]
ChEMBL	Inhibition of human full-length C-terminal His-tagged HDAC8 expressed in baculovirus infected Sf9 insect cell using fluorogenic HDAC class 2a substrate measured after 30 mins by fluorimetry	B	6.51	pIC50	310	nM	IC50	Bioorg Med Chem (2022) 56: 116599-116599 [PMID:35041998]
ChEMBL	Inhibition of human recombinant C-terminal His-tagged HDAC8 (1 to 377 residues) expressed in insect cells using RHK-K(Ac)-K(Ac)-AMC as substrate incubated for 60 mins by fluorescence assay	B	6.51	pIC50	307.4	nM	IC50	Eur J Med Chem (2016) 116: 126-135 [PMID:27060764]
ChEMBL	Inhibition of HDAC8	B	6.52	pIC50	300	nM	IC50	Bioorg Med Chem (2010) 18: 4103-4110 [PMID:20472442]
ChEMBL	Inhibition of HDAC8 (unknown origin) by colorimetric method	B	6.54	pIC50	290	nM	IC50	Eur J Med Chem (2022) 240: 114602-114602 [PMID:35858522]
ChEMBL	Inhibition of human recombinant HDAC8 after 30 mins using fluorogenic substrate by fluorimetric assay	B	6.55	pIC50	280	nM	IC50	J Med Chem (2018) 61: 6574-6591 [PMID:30004697]
ChEMBL	Inhibition of recombinant full-length human HDAC8 using FAM-RHKK(Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	6.62	pIC50	240	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of recombinant full-length human HDAC8 using RHKK(Ac) as substrate measured after 2 hrs by fluorescence assay	B	6.63	pIC50	232	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of human HDAC8 by fluorescence assay	B	6.71	pIC50	196	nM	IC50	J Med Chem (2013) 56: 427-436 [PMID:23252603]
ChEMBL	Inhibition of HDAC8 (unknown origin) using RHK(Ac)K(Ac) fluorogenic acetylated peptide substrate by fluorometric assay	B	6.77	pIC50	170	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	6.78	pIC50	165	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of HDAC8 (unknown origin) incubated for 1 hr by fluor de lys substrate based fluorescence method	B	6.8	pIC50	160	nM	IC50	Bioorg Med Chem (2015) 23: 6785-6793 [PMID:26481659]
ChEMBL	Inhibition of human recombinant HDAC8 expressed in baculovirus/sf9 cells using RHKAcKAc as substrate	B	6.89	pIC50	129	nM	IC50	J Med Chem (2013) 56: 6775-6791 [PMID:23905680]
ChEMBL	Inhibition of HDAC8 (unknown origin) by ELISA	B	6.9	pIC50	125	nM	IC50	Eur J Med Chem (2018) 148: 73-85 [PMID:29454918]
ChEMBL	Inhibition of HDAC8 by in vitro deacetylation assay	B	7	pIC50	100	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
ChEMBL	Inhibition of human HDAC8	B	7.05	pIC50	90	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of human HDAC8 by fluorimetric assay	B	7.05	pIC50	89.53	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC8 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.05	pIC50	89.5	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human histone deacetylase 8 prepared from 293T cells	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2004) 14: 5343-5346 [PMID:15454224]
ChEMBL	Inhibition of human recombinant HDAC8 after 30 mins by fluorimetric assay	B	7.6	pIC50	25	nM	IC50	J Med Chem (2009) 52: 7836-7846 [PMID:19705846]
ChEMBL	Inhibition of HDAC8 after 17 hrs	B	8.86	pIC50	1.38	nM	IC50	J Med Chem (2008) 51: 4370-4373 [PMID:18642892]
histone deacetylase 8/Histone deacetylase 8 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2347] [GtoPdb: 2619] [UniProtKB: Q8VH37]
ChEMBL	Inhibitory activity against histone deacetylase 8 prepared from mouse melanoma B16/BL6 cells	B	7.4	pIC50	40	nM	IC50	Bioorg Med Chem Lett (2004) 14: 2427-2431 [PMID:15109626]
histone deacetylase 9/Histone deacetylase 9 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4145] [GtoPdb: 2620] [UniProtKB: Q9UKV0]
ChEMBL	Inhibition of human HDAC9	B	6.1	pKi	800	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of human HDAC9	B	6.1	pKi	800	nM	Ki	Bioorg Med Chem (2015) 23: 5151-5155 [PMID:25637120]
GtoPdb	-	-	6.1	pKi	800	nM	Ki	Nat Chem Biol (2010) 6: 238-243 [PMID:20139990]
ChEMBL	Inhibition of recombinant full-length human HDAC9 using FAM-RHKK (trifluor-Ac)-NH2 as substrate by electrophoretic mobility shift assay	B	5.27	pIC50	5370	nM	IC50	J Med Chem (2023) 66: 16075-16090 [PMID:37972387]
ChEMBL	Inhibition of HDAC9 (unknown origin) using Boc-Lys(trifluoroacetyl)-AMC fluorogenic acetylated peptide substrate by fluorometric assay	B	5.3	pIC50	5000	nM	IC50	Bioorg Med Chem Lett (2015) 25: 4320-4324 [PMID:26264503]
ChEMBL	Inhibition of recombinant full-length human HDAC9 using Boc-Lys(trifluoroacetyl)-AMC as substrate measured after 2 hrs by fluorescence assay	B	5.31	pIC50	4920	nM	IC50	Eur J Med Chem (2018) 158: 620-706 [PMID:30245394]
ChEMBL	Inhibition of recombinant human HDAC9 (604-1066 residues) expressed in baculovirus infected Sf9 cells using FAM-RHKK-TFAc as substrate incubated for 3 hrs by electrophoretic mobility shift assay	B	6	pIC50	>1000	nM	IC50	ACS Med Chem Lett (2020) 11: 56-64 [PMID:31938464]
ChEMBL	HDAC enzyme inhibitionAssay: HDAC enzyme inhibition assays were performed using purified HDACs 1-10 essentially as described in Beckers et al., 2007, Int. J. Cancer., 121:1138-48 and Perez-Balado et al., 2007, J. Med. Chem., 50:2497-2505. Inhibition assays using nuclear extract were performed essentially as described in Herman et al., 2006, Nat. Chem. Biol., 2:551-558. Briefly, the purified HDACs or nuclear extract were incubated with an acetylated substrate in the absence of the compound to be assayed and with increasing concentrations of the compound. The rate of substrate deacetylation was measured under each condition, and half-maximal inhibitory concentration with regard to each HDAC was determined by standard means.	B	7.15	pIC50	70.1	nM	IC50	US-9265734-B2. Compositions including 6-aminohexanoic acid derivatives as HDAC inhibitors (2016)
ChEMBL	Inhibition of human HDAC9 by fluorimetric assay	B	7.42	pIC50	38.12	nM	IC50	J Med Chem (2010) 53: 8387-8399 [PMID:21073160]
ChEMBL	Inhibition of human HDAC9 using fluorogenic tetrapeptide RHKKAc as substrate	B	7.42	pIC50	38.1	nM	IC50	Eur J Med Chem (2014) 79: 251-259 [PMID:24742384]
ChEMBL	Inhibition of human HDAC9	B	7.42	pIC50	38	nM	IC50	Bioorg Med Chem Lett (2009) 19: 2346-2349 [PMID:19285395]
ChEMBL	Inhibition of human recombinant HDAC9 using fluorophore-conjugated substrate Boc-L-Lys(Ac)-AMC after 60 mins	B	7.7	pIC50	20.1	nM	IC50	J Med Chem (2011) 54: 4350-4364 [PMID:21548582]
ChEMBL	Inhibition of HDAC9 by in vitro deacetylation assay	B	7.7	pIC50	20	nM	IC50	Nat Chem Biol (2010) 6: 25-33 [PMID:19966789]
Histone deacetylase HD1B in Zea mays (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3541] [UniProtKB: Q9ZTP8]
ChEMBL	Inhibition of maize HD1B	B	9.4	pIC50	0.4	nM	IC50	J Med Chem (2006) 49: 6046-6056 [PMID:17004718]
ChEMBL	Inhibition of maize histone deacetylase 1B	B	9.4	pIC50	0.4	nM	IC50	Eur J Med Chem (2008) 43: 621-632 [PMID:17698257]
Histone deacetylase HD2 in Zea mays (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4919] [UniProtKB: Q94F81]
ChEMBL	Binding affinity against Histone deacetylase 2 (HD2) in maize, expressed as binding constant (pKi)	B	8.36	pKi	4.37	nM	Ki	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Experimental anti-HDAC (anti-histone deacetylase) activity of the compound	B	8.36	pKi	4.37	nM	Ki	J Med Chem (2001) 44: 2069-2072 [PMID:11405644]
ChEMBL	Inhibition of maize histone deacetylase 2	B	8.14	pIC50	7.2	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	Inhibitory concentration against histone deacetylase activity	B	8.14	pIC50	7.2	nM	IC50	J Med Chem (2001) 44: 2069-2072 [PMID:11405644]
ChEMBL	Concentration required for inhibition of histone deacetylase HD2 in vitro.	B	8.14	pIC50	7.2	nM	IC50	J Med Chem (2004) 47: 1098-1109 [PMID:14971890]
ChEMBL	Inhibitory activity against maize Histone deacetylase 2 at 1 mM	B	8.14	pIC50	7.2	nM	IC50	J Med Chem (2003) 46: 4826-4829 [PMID:14584932]
ChEMBL	Inhibition of maize Histone deacetylase 2	B	8.15	pIC50	7.08	nM	IC50	J Med Chem (2004) 47: 1351-1359 [PMID:14998325]
ChEMBL	Inhibition of maize HD2	B	8.15	pIC50	7	nM	IC50	J Med Chem (2006) 49: 6046-6056 [PMID:17004718]
ChEMBL	In vitro for anti-HD2 (Histone deacetylase 2) activity in maize	B	8.15	pIC50	7	nM	IC50	J Med Chem (2002) 45: 1778-1784 [PMID:11960489]
ChEMBL	Inhibitory concentration against maize histone deacetylase 2	B	8.15	pIC50	7	nM	IC50	J Med Chem (2005) 48: 3344-3353 [PMID:15857140]
ChEMBL	Inhibition of maize Histone deacetylase 2 (HD-2) activity	B	8.52	pIC50	3	nM	IC50	J Med Chem (1999) 42: 4669-4679 [PMID:10579829]
ChEMBL	Inhibitory concentration against maize Histone deacetylase 2	B	8.52	pIC50	3	nM	IC50	J Med Chem (2003) 46: 5097-5116 [PMID:14613312]
ChEMBL	Inhibitory concentration against maize Histone deacetylase 2	B	8.52	pIC50	3	nM	IC50	J Med Chem (2003) 46: 5097-5116 [PMID:14613312]
ChEMBL	Inhibition of in vitro enzyme activity measured in a highly purified maize Histone deacetylase 2 preparation	B	8.52	pIC50	3	nM	IC50	J Med Chem (2002) 45: 3296-3309 [PMID:12109913]
Histone deacetylase-like amidohydrolase in Alcaligenes sp. (strain DSM 11172) (Bordetella sp. (strain FB188)) (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6017] [UniProtKB: Q70I53]
ChEMBL	Displacement of Atto700-HA from Bordetella / Alcaligenes strain FB188 HDAH by fluorescence anisotropy	B	6.15	pKi	700	nM	Ki	Bioorg Med Chem Lett (2009) 19: 3651-3656 [PMID:19457659]
ChEMBL	Inhibition of Bordetella / Alcaligenes strain FB188 HDAH by fluorimetric assay	B	5.92	pIC50	1200	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3651-3656 [PMID:19457659]
Leukotriene A4 hydrolase/Leukotriene A4 hydrolase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4618] [GtoPdb: 1395] [UniProtKB: P09960]
ChEMBL	Inhibition of recombinant human LTA4H Epoxide Hydrolase expressed in Escherichia coli BL21 (DE3) pLysS preincubated for 10 mins followed by addition of LTA4 as substrate measured after 15 mins by reverse-phase HPLC analysis	B	5	pIC50	>10000	nM	IC50	J Med Chem (2017) 60: 1817-1828 [PMID:28218840]
lysine demethylase 4E/Lysine-specific demethylase 4D-like in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293226] [GtoPdb: 2679] [UniProtKB: B2RXH2]
ChEMBL	Inhibition of KDM4E (unknown origin)	B	4.55	pIC50	28400	nM	IC50	J Med Chem (2022) 65: 9564-9579 [PMID:35838529]
sirtuin 6/NAD-dependent protein deacetylase sirtuin-6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2163182] [GtoPdb: 2712] [UniProtKB: Q8N6T7]
ChEMBL	Binding affinity to recombinant human full length SIRT6 (1 to 355 residues) expressed in Escherichia coli M15 after 20 mins in presence of ADP-ribose by fluorescence-based SDS-denaturation assay	B	4.48	pKd	33000	nM	Kd	J Med Chem (2018) 61: 10922-10928 [PMID:30395713]
ChEMBL	Binding affinity to recombinant human full length SIRT6 (1 to 355 residues) expressed in Escherichia coli M15 after 20 mins in presence of ADP-ribose and acetylated H3K9 peptide by fluorescence-based SDS-denaturation assay	B	4.54	pKd	29000	nM	Kd	J Med Chem (2018) 61: 10922-10928 [PMID:30395713]
ChEMBL	Inhibition of SIRT6 deacetylation (unknown origin) using p53K382Ac peptide as substrate	B	5.34	pKi	4620	nM	Ki	J Med Chem (2021) 64: 9732-9758 [PMID:34213345]
ChEMBL	Inhibition of SIRT6 deacetylation (unknown origin) using H3K9Ac peptide as substrate	B	5.69	pKi	2020	nM	Ki	J Med Chem (2021) 64: 9732-9758 [PMID:34213345]
ChEMBL	Inhibition of full length SIRT6 (unknown origin) using acetylated H3K9 peptide as substrate after 2 hrs in presence of NAD	B	5.7	pKi	2000	nM	Ki	J Med Chem (2018) 61: 10922-10928 [PMID:30395713]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 by [3H]hypoxanthine uptake	F	7.8	pIC50	16	nM	IC50	J Med Chem (2009) 52: 2185-2187 [PMID:19317450]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 after 48 hrs by LDH reporter assay	F	7.81	pIC50	15.6	nM	IC50	Bioorg Med Chem (2008) 16: 5254-5265 [PMID:18362073]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum D6 after 48 hrs by LDH reporter assay	F	7.81	pIC50	15.49	nM	IC50	Bioorg Med Chem (2008) 16: 5254-5265 [PMID:18362073]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in O+ human erythrocytes by [3H]hypoxanthine incorporation assay	F	7.96	pIC50	11	nM	IC50	Antimicrob Agents Chemother (2008) 52: 1454-1461 [PMID:18212103]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 after 48 hrs by LDH reporter assay	F	8	pIC50	10	nM	IC50	Bioorg Med Chem (2008) 16: 5254-5265 [PMID:18362073]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum W2 after 48 hrs by LDH reporter assay	F	8.01	pIC50	9.77	nM	IC50	Bioorg Med Chem (2008) 16: 5254-5265 [PMID:18362073]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 infected in O+ human erythrocytes by [3H]hypoxanthine incorporation assay	F	8.1	pIC50	8	nM	IC50	Antimicrob Agents Chemother (2008) 52: 1454-1461 [PMID:18212103]
ChEMBL	Antimalarial activity against Plasmodium falciparum infected in erythrocytes after 2 hrs	F	8.1	pIC50	8	nM	IC50	Eur J Med Chem (2014) 82: 204-213 [PMID:24904967]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum	F	8.1	pIC50	8	nM	IC50	Eur J Med Chem (2018) 158: 801-813 [PMID:30245402]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum 3D7 (drug-susceptible) proliferation in erythrocyte-based infection assay	F	6.7	pEC50	200.2	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum W2 (drug-resistant) proliferation in erythrocyte-based infection assay	F	6.77	pEC50	170.8	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
Splicing factor 3B subunit 3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1250378] [UniProtKB: Q15393]
ChEMBL	Inhibition of SAP130 in VEGF-stimulated human U251 cells by PLAP reporter gene assay	B	6.21	pIC50	611.2	nM	IC50	Nat Chem Biol (2007) 3: 570-575 [PMID:17643112]
ChEMBL	Inhibition of SAP130 mediated cell growth in human WiDr cells	B	6.24	pIC50	577.6	nM	IC50	Nat Chem Biol (2007) 3: 570-575 [PMID:17643112]
Vitamin D receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1977] [GtoPdb: 605] [UniProtKB: P11473]
ChEMBL	Agonist activity at Vitamin D receptor (unknown origin) by fluorescence polarization assay	B	7.06	pIC50	87	nM	IC50	Eur J Med Chem (2021) 209: 112904-112904 [PMID:33077264]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]