ChEMBL ligand: CHEMBL973 (A77 1726, Aubagio, HMR-1726, HMR1726, Leflunomide related compound b, Leflunomide related compound b rs, Teriflunomide)
dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] There should be some charts here, you may need to enable JavaScript!
Dihydroorotate dehydrogenase in Plasmodium falciparum [ChEMBL: CHEMBL3486] [UniProtKB: Q54A96] dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Human [ChEMBL: CHEMBL1966] [GtoPdb: 2604] [UniProtKB: Q02127] dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Mouse [ChEMBL: CHEMBL2991] [GtoPdb: 2604] [UniProtKB: O35435] dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Rat [ChEMBL: CHEMBL2383] [GtoPdb: 2604] [UniProtKB: Q63707] There should be some charts here, you may need to enable JavaScript!
Dihydroorotate dehydrogenase (quinone), mitochondrial in Schistosoma mansoni [ChEMBL: CHEMBL4523950] [UniProtKB: G4VFD7] There should be some charts here, you may need to enable JavaScript!
Orai1/ORAI1/STIM1 in Human [ChEMBL: CHEMBL3832644] [GtoPdb: 2964] [UniProtKB: Q13586, Q96D31] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
dihydrofolate reductase/Dihydrofolate reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374]
ChEMBL	Inhibition of recombinant human N-terminal truncated GST-tagged DHFR (31 to 395 residues) expressed in Escherichia coli BL21(DE3) pyrD using DHO as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by DCIP oxidation based CoQ10 enzyme coupled assay	B	6.41	pIC50	388	nM	IC50	Eur J Med Chem (2017) 129: 287-302 [PMID:28235702]
Dihydroorotate dehydrogenase in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3486] [UniProtKB: Q54A96]
ChEMBL	Inhibition of recombinant Plasmodium falciparum DHODH	B	4.63	pKi	23350	nM	Ki	J Med Chem (2007) 50: 186-191 [PMID:17228860]
ChEMBL	Inhibition of ubiquinone binding site of Plasmodium falciparum dihydroorotate dehydrogenase	B	4.66	pKi	22000	nM	Ki	J Med Chem (2009) 52: 2683-2693 [PMID:19351152]
ChEMBL	Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli BL21 (DE3) using L-dihydroorotate as substrate after 10 mins by DCIP dye reduction assay	B	4.94	pIC50	11390	nM	IC50	J Med Chem (2012) 55: 8341-8349 [PMID:22984987]
ChEMBL	Inhibition of N-terminal His6-tagged recombinant Plasmodium falciparum DHODH (amino acid residues 158 to 569) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	B	5.41	pIC50	3900	nM	IC50	J Med Chem (2014) 57: 5381-5394 [PMID:24801997]
dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1966] [GtoPdb: 2604] [UniProtKB: Q02127]
ChEMBL	Inhibition of dihydroorotate dehydrogenase	B	5.57	pKi	2700	nM	Ki	J Med Chem (2001) 44: 281-297 [PMID:11462969]
ChEMBL	Inhibition of ubiquinone binding site of human dihydroorotate dehydrogenase	B	7.49	pKi	32	nM	Ki	J Med Chem (2009) 52: 2683-2693 [PMID:19351152]
GtoPdb	-	-	7.52	pKi	30	nM	Ki	J Med Chem (2007) 50: 186-91 [PMID:17228860]
ChEMBL	Inhibition of human recombinant DHODH	B	7.52	pKi	30	nM	Ki	J Med Chem (2007) 50: 186-191 [PMID:17228860]
ChEMBL	Inhibition of His-tagged human DHODH assessed as reduction of DCIP by spectrophotometry	B	5.3	pIC50	5011.87	nM	IC50	J Med Chem (2015) 58: 860-877 [PMID:25558988]
ChEMBL	Inhibition of recombinant N-terminal His-tagged human DHODH (31 to 395 residues) expressed in Escherichia coli using DL-dihydroorotic acid as substrate and Q0 as coenzyme by DCIP based assay	B	5.96	pIC50	1100	nM	IC50	J Med Chem (2021) 64: 13918-13932 [PMID:34516133]
ChEMBL	Inhibition of human dihydroorotate dehydrogenase	B	5.97	pIC50	1083	nM	IC50	Bioorg Med Chem Lett (2011) 21: 7268-7272 [PMID:22078215]
ChEMBL	Inhibition of human recombinant DHODH	B	6	pIC50	1000	nM	IC50	J Med Chem (2015) 58: 5579-5598 [PMID:26079043]
ChEMBL	Inhibition of C-terminal His6-tagged human DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	B	6.36	pIC50	440	nM	IC50	J Med Chem (2014) 57: 5381-5394 [PMID:24801997]
ChEMBL	Inhibition of N-terminally truncated recombinant human dihydroorotate dehydrogenase using in vitro enzyme assay	B	6.38	pIC50	420	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4854-4857 [PMID:16143532]
ChEMBL	Inhibition of recombinant N-terminal GST-tagged human DHODH (31 to 395 residues) expressed in Escherichia coli BL21(DE3) assessed as inhibition of DCIP reduction using dihydroorotate as substrate preincubated for 5 mins followed by substrate addition measured after 5 mins	B	6.41	pIC50	388	nM	IC50	J Med Chem (2018) 61: 6034-6055 [PMID:29939742]
ChEMBL	Inhibition of human DHODH assessed as decrease in DCIP using dihydroorotate as substrate measured every 30 seconds for 6 mins	B	6.45	pIC50	356	nM	IC50	Bioorg Med Chem Lett (2016) 26: 3064-3066 [PMID:27220723]
ChEMBL	Inhibition of human DHODH	B	6.49	pIC50	320	nM	IC50	Eur J Med Chem (2019) 183: 111681-111681 [PMID:31557612]
GtoPdb	-	-	6.51	pIC50	307.1	nM	IC50	Protein Cell (2020) 11: 723-739 [PMID:32754890]
ChEMBL	Inhibition of ubiquinone binding site of human dihydroorotate dehydrogenase	B	6.58	pIC50	261	nM	IC50	J Med Chem (2009) 52: 2683-2693 [PMID:19351152]
ChEMBL	Inhibition of human recombinant DHODH	B	6.59	pIC50	260	nM	IC50	J Med Chem (2007) 50: 186-191 [PMID:17228860]
ChEMBL	Inhibition of human recombinant DHODH expressed in Escherichia coli BL21 (DE3) using dihydroorotate as substrate and CoQ6 as co-substrate incubated for 10 mins by DCIP based multimode microplate reader analysis	B	6.64	pIC50	227	nM	IC50	J Med Chem (2022) 65: 592-615 [PMID:34957834]
ChEMBL	Inhibition of C-terminal His6-tagged human DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by direct assay in presence of oxygen depleting system	B	6.68	pIC50	210	nM	IC50	J Med Chem (2014) 57: 5381-5394 [PMID:24801997]
ChEMBL	Inhibition of human DHODH using dihydroorotate substrate by DCIP assay	B	6.79	pIC50	163	nM	IC50	J Med Chem (2015) 58: 1123-1139 [PMID:25580811]
ChEMBL	Inhibition of N-terminal His10-tagged human DHODH (Met30 to Arg396) expressed in Escherichia coli BL21 (DE3) using dihydroorotate as substrate measured every 30 secs for 6 mins by DCIP dye-based assay	B	6.86	pIC50	137	nM	IC50	J Med Chem (2013) 56: 7911-7924 [PMID:24073986]
ChEMBL	Inhibition of human DHODH expressed in Escherichia coli BL21(DE3) using L-dihydroorotate as substrate after 10 mins by DCIP dye reduction assay	B	6.89	pIC50	130	nM	IC50	J Med Chem (2012) 55: 8341-8349 [PMID:22984987]
ChEMBL	Inhibition of DHODH in human HEK-293 cells transfected with ISRE-luciferase reporter gene assessed as increase in IFN-alpha mediated cellular response upto 72 hrs by luciferase reporter gene assay	B	5.05	pEC50	8912.51	nM	EC50	Eur J Med Chem (2020) 186: 111855-111855 [PMID:31740051]
ChEMBL	Inhibition of DHODH in human HEK-293 cells transfected with ISRE-luciferase reporter gene assessed as increase in IFN-alpha mediated cellular response upto 72 hrs by luciferase reporter gene assay	B	5.05	pEC50	8900	nM	EC50	Eur J Med Chem (2020) 186: 111855-111855 [PMID:31740051]
dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2991] [GtoPdb: 2604] [UniProtKB: O35435]
ChEMBL	Inhibitory activity against mouse dihydroorotate dehydrogenase	B	6.81	pIC50	156	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4854-4857 [PMID:16143532]
ChEMBL	Inhibition of mouse DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	B	6.82	pIC50	150	nM	IC50	J Med Chem (2014) 57: 5381-5394 [PMID:24801997]
ChEMBL	Inhibitory concentration tested on enzyme dihydroorotate dehydrogenase in mouse	B	7.16	pIC50	69	nM	IC50	J Med Chem (1996) 39: 4608-4621 [PMID:8917650]
dihydroorotate dehydrogenase (quinone)/Dihydroorotate dehydrogenase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2383] [GtoPdb: 2604] [UniProtKB: Q63707]
ChEMBL	Inhibition of rat dihydroorotate dehydrogenase	B	7.59	pIC50	26	nM	IC50	Bioorg Med Chem Lett (2011) 21: 7268-7272 [PMID:22078215]
ChEMBL	Inhibition of DHODH in Wistar rat liver homogenates by DCIP reduction assay	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2011) 46: 383-392 [PMID:21109332]
ChEMBL	Inhibition of dihydroorotate dehydrogenase in Wistar rat liver mitochondrial/microsomal membranes measured for 5 mins by 2,6-dichlorophenolindophenol reduction-based spectrophotometry	B	7.7	pIC50	20	nM	IC50	Eur J Med Chem (2012) 49: 102-109 [PMID:22245049]
ChEMBL	Inhibition of rat DHODH (amino acid residues 30 to 396) expressed in Escherichia coli BL21 cells assessed as orotic acid production using dihydroorotate as substrate by DCIP dye-based assay	B	7.74	pIC50	18	nM	IC50	J Med Chem (2014) 57: 5381-5394 [PMID:24801997]
ChEMBL	Inhibitory concentration tested against enzyme dihydroorotate dehydrogenase in rat	B	7.89	pIC50	13	nM	IC50	J Med Chem (1996) 39: 4608-4621 [PMID:8917650]
ChEMBL	Inhibitory activity against rat dihydroorotate dehydrogenase	B	8.05	pIC50	9	nM	IC50	Bioorg Med Chem Lett (2005) 15: 4854-4857 [PMID:16143532]
Dihydroorotate dehydrogenase (quinone), mitochondrial in Schistosoma mansoni (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4523950] [UniProtKB: G4VFD7]
ChEMBL	Inhibition of Schistosoma mansoni DHODH using DHO as substrate measured at 4 secs interval for 60 secs by DCIP reduction based indirect assay	B	4.3	pIC50	50000	nM	IC50	Eur J Med Chem (2019) 167: 357-366 [PMID:30776695]
ChEMBL	Inhibition of Schistosoma mansoni DHODH	B	4.3	pIC50	50000	nM	IC50	Eur J Med Chem (2019) 183: 111681-111681 [PMID:31557612]
Orai1/ORAI1/STIM1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3832644] [GtoPdb: 2964] [UniProtKB: Q13586, Q96D31]
ChEMBL	Inhibition of STIM1/Orai1 (unknown origin) expressed in HEK293 cells assessed as reduction in tBHQ-induced calcium response	B	5.37	pIC50	4300	nM	IC50	J Med Chem (2020) 63: 14761-14779 [PMID:33253576]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]