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ChEMBL ligand: CHEMBL428496 (Wortmannin) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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protein kinase, DNA-activated, catalytic subunit/DNA-dependent protein kinase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3142] [GtoPdb: 2800] [UniProtKB: P78527] | ||||||||
GtoPdb | - | - | 6.92 | pKi | 120 | nM | Ki | Bioorg Med Chem Lett (2012) 22: 5352-9 [PMID:22835870] |
ChEMBL | Binding affinity for DNA dependent protein kinase isolated from HeLa cells; Range is 20-120 | B | 6.92 | pKi | 120 | nM | Ki | J Med Chem (2005) 48: 569-585 [PMID:15658870] |
ChEMBL | Inhibition of DNA-PK isolated from human HeLa cell extract | B | 6.92 | pKi | 120 | nM | Ki | Bioorg Med Chem Lett (2012) 22: 5352-5359 [PMID:22835870] |
Myosin light chain kinase, smooth muscle in Chicken (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3062] [UniProtKB: P11799] | ||||||||
ChEMBL | Inhibition of chicken SmMLCK expressed in Escherichia coli | B | 6.59 | pIC50 | 260 | nM | IC50 | Biochem J (2000) 351: 95-105 [PMID:10998351] |
myosin light chain kinase/Myosin light chain kinase, smooth muscle in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2428] [GtoPdb: 1552] [UniProtKB: Q15746] | ||||||||
ChEMBL | Inhibition of SmMLCK in the presence of 50uM ATP | B | 5.3 | pIC50 | 5000 | nM | IC50 | Biochem J (2007) 408: 297-315 [PMID:17850214] |
ChEMBL | Inhibition of Smooth muscle myosin light chain kinase (mMLCK) | B | 5.72 | pIC50 | 1900 | nM | IC50 | J Med Chem (2003) 46: 1478-1483 [PMID:12672248] |
ChEMBL | Inhibition of MLCK by HTRF assay | B | 6.77 | pIC50 | 170 | nM | IC50 | Bioorg Med Chem (2011) 19: 429-439 [PMID:21130659] |
phosphoinositide-3-kinase regulatory subunit 1/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha/p85-alpha in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111367] [GtoPdb: 2503, 2153] [UniProtKB: P27986, P42336] | ||||||||
GtoPdb | - | - | 8.27 | pKd | 5.4 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 6.92 | pKi | 120 | nM | Ki | J Med Chem (2005) 48: 569-85 [PMID:15658870] |
ChEMBL | Inhibition of PI3K p110alpha/p85alpha (unknown origin) by ADP-Glo assay | B | 5.79 | pIC50 | 1610 | nM | IC50 | Eur J Med Chem (2013) 60: 187-198 [PMID:23291120] |
ChEMBL | Inhibition of human recombinant P110alpha/p85alpha using phosphatidyl inositol as substrate after 1 hr by ADP-Glo Kinase assay | B | 7.92 | pIC50 | 11.9 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 6919-6922 [PMID:23040731] |
ChEMBL | Inhibition of human full-length recombinant p110alpha/p85alpha co-expressed in baculovirus expression system using PIP2:PS lipid as substrate incubated for 1 hr in presence of ATP by ADP-Glo assay | B | 8 | pIC50 | 10.1 | nM | IC50 | J Med Chem (2018) 61: 1552-1575 [PMID:29360358] |
ChEMBL | Inhibition of PI3 kinase p110alpha/p85-alpha complex | B | 8.92 | pIC50 | 1.2 | nM | IC50 | Bioorg Med Chem Lett (2006) 16: 2518-2521 [PMID:16464575] |
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4005] [GtoPdb: 2153] [UniProtKB: P42336] | ||||||||
ChEMBL | Binding affinity to human PI3Kalpha after 1 hr by KINOMEscan assay | B | 8.27 | pKd | 5.4 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 8.27 | pKd | 5.4 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 6.92 | pKi | 120 | nM | Ki | J Med Chem (2005) 48: 569-85 [PMID:15658870] |
ChEMBL | Binding affinity for Phosphatidylinositol 3-kinase isolated from HeLa cells; Range is 20-120 | B | 6.92 | pKi | 120 | nM | Ki | J Med Chem (2005) 48: 569-585 [PMID:15658870] |
ChEMBL | Inhibition of PI3K p110alpha (unknown origin) using PIP2 as substrate preincubated for 10 mins followed by substrate addition measured after 1 hr | B | 6 | pIC50 | >=1000 | nM | IC50 | Bioorg Med Chem Lett (2017) 27: 2510-2514 [PMID:28462836] |
ChEMBL | Inhibition of PI3K by fluorescence energy transfer assay | B | 7.75 | pIC50 | 17.6 | nM | IC50 | J Med Chem (2008) 51: 4699-4707 [PMID:18630894] |
ChEMBL | Inhibition of human p110alpha PI3K fragment by AlphaScreen assay | B | 7.89 | pIC50 | 13 | nM | IC50 | Bioorg Med Chem Lett (2011) 21: 829-835 [PMID:21169017] |
ChEMBL | Inhibition of human PI3Kalpha expressed in Sf9 cells by fluorescent polarization assay | B | 7.92 | pIC50 | 12 | nM | IC50 | J Med Chem (2010) 53: 8523-8533 [PMID:21121631] |
ChEMBL | Inhibition of PI3Kalpha (unknown origin) by fluorescent polarization assay | B | 7.92 | pIC50 | 12 | nM | IC50 | Medchemcomm (2012) 3: 1337-1355 |
ChEMBL | Inhibition of PI3 kinase | B | 7.92 | pIC50 | 12 | nM | IC50 | Bioorg Med Chem Lett (2009) 19: 4223-4227 [PMID:19523825] |
ChEMBL | Inhibition of human PI3Kalpha | B | 9 | pIC50 | 1 | nM | IC50 | J Med Chem (2006) 49: 3857-3871 [PMID:16789742] |
ChEMBL | Inhibition of PI3Kalpha | B | 9 | pIC50 | 1 | nM | IC50 | J Med Chem (2012) 55: 8559-8581 [PMID:22924688] |
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3-kinase p110-beta subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3145] [GtoPdb: 2154] [UniProtKB: P42338] | ||||||||
ChEMBL | Binding affinity to human PI3Kbeta after 1 hr by KINOMEscan assay | B | 8.12 | pKd | 7.6 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 8.12 | pKd | 7.6 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
ChEMBL | Inhibition of human PI3K p110beta catalytic subunit by AlphaScreen competition assay | B | 7.82 | pIC50 | 15 | nM | IC50 | Bioorg Med Chem Lett (2011) 21: 829-835 [PMID:21169017] |
ChEMBL | Inhibition of PI3Kbeta | B | 7.85 | pIC50 | 14 | nM | IC50 | J Med Chem (2012) 55: 8559-8581 [PMID:22924688] |
ChEMBL | Inhibition of human PI3Kbeta | B | 8 | pIC50 | 10 | nM | IC50 | J Med Chem (2006) 49: 3857-3871 [PMID:16789742] |
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta/PI3-kinase p110-delta subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3130] [GtoPdb: 2155] [UniProtKB: O00329] | ||||||||
ChEMBL | Binding affinity to human PI3Kdelta after 1 hr by KINOMEscan assay | B | 8.26 | pKd | 5.5 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 8.26 | pKd | 5.5 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
ChEMBL | Inhibition of human PI3Kdelta | B | 8.05 | pIC50 | 9 | nM | IC50 | J Med Chem (2006) 49: 3857-3871 [PMID:16789742] |
ChEMBL | Inhibition of PI3Kdelta | B | 8.05 | pIC50 | 9 | nM | IC50 | J Med Chem (2012) 55: 8559-8581 [PMID:22924688] |
ChEMBL | Inhibition of human PI3K p110delta catalytic subunit by AlphaScreen assay | B | 8.4 | pIC50 | 4 | nM | IC50 | Bioorg Med Chem Lett (2011) 21: 829-835 [PMID:21169017] |
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma/PI3-kinase p110-gamma subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3267] [GtoPdb: 2156] [UniProtKB: P48736] | ||||||||
GtoPdb | - | - | 7.82 | pKd | 15 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
ChEMBL | Binding affinity to human PI3Kgamma after 1 hr by KINOMEscan assay | B | 7.82 | pKd | 15 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
ChEMBL | Inhibition of PI3Kgamma (unknown origin) assessed as decrease in fluorescence intensity using phosphorylated substrate | B | 4.26 | pIC50 | 55000 | nM | IC50 | Eur J Med Chem (2014) 84: 454-465 [PMID:25050878] |
ChEMBL | Inhibition of human PI3K p110gamma catalytic subunit by AlphaScreen assay | B | 7.05 | pIC50 | 89 | nM | IC50 | Bioorg Med Chem Lett (2011) 21: 829-835 [PMID:21169017] |
ChEMBL | Inhibition of PI3Kgamma assessed as inhibition of 32P-PIP3 formation | B | 7.72 | pIC50 | 19 | nM | IC50 | Bioorg Med Chem Lett (2007) 17: 756-760 [PMID:17095227] |
ChEMBL | Inhibition of human PI3Kgamma | B | 8.3 | pIC50 | 5 | nM | IC50 | J Med Chem (2006) 49: 3857-3871 [PMID:16789742] |
ChEMBL | Inhibition of PI3Kgamma | B | 8.3 | pIC50 | 5 | nM | IC50 | J Med Chem (2012) 55: 8559-8581 [PMID:22924688] |
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364] | ||||||||
ChEMBL | Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum Dd2 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay | F | 5 | pIC50 | 10000 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay | F | 5 | pIC50 | 10000 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
mechanistic target of rapamycin kinase/Serine/threonine-protein kinase mTOR in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2842] [GtoPdb: 2109] [UniProtKB: P42345] | ||||||||
ChEMBL | Binding affinity to human mTOR after 1 hr by KINOMEscan assay | B | 5.04 | pKd | 9200 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
GtoPdb | - | - | 5.04 | pKd | 9200 | nM | Kd | J Med Chem (2018) 61: 10463-10472 [PMID:30380865] |
ChEMBL | Inhibitory activity against MTOR | B | 7.4 | pIC50 | 40 | nM | IC50 | Bioorg Med Chem Lett (2005) 15: 4799-4802 [PMID:16143526] |
polo like kinase 1/Serine/threonine-protein kinase PLK1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3024] [GtoPdb: 2168] [UniProtKB: P53350] | ||||||||
ChEMBL | Inhibition of PLK1 in the presence of 5uM ATP | B | 5.89 | pIC50 | 1300 | nM | IC50 | Biochem J (2007) 408: 297-315 [PMID:17850214] |
ChEMBL | Inhibition of GST-tagged Plk1 using recombinant protein X as a substrate containing threonine residues | B | 7.62 | pIC50 | 24 | nM | IC50 | J Med Chem (2012) 55: 6243-6262 [PMID:22621397] |
ChEMBL | Inhibition of AX-7503 binding to recombinant Plk1 by Western blot | B | 8.24 | pIC50 | 5.8 | nM | IC50 | J Biol Chem (2007) 282: 2505-2511 [PMID:17135248] |
polo like kinase 3/Serine/threonine-protein kinase PLK3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4897] [GtoPdb: 2170] [UniProtKB: Q9H4B4] | ||||||||
ChEMBL | Inhibition of Plk3 in human A549 cells assessed as casein substrate phosphorylation by Western blot | B | 6.66 | pIC50 | 220 | nM | IC50 | J Biol Chem (2007) 282: 2505-2511 [PMID:17135248] |
ChEMBL | Inhibition of AX-7503 binding to recombinant Plk3 expressed in HeLa cells by Western blot | B | 7.31 | pIC50 | 49 | nM | IC50 | J Biol Chem (2007) 282: 2505-2511 [PMID:17135248] |
ChEMBL | Inhibition of recombinant Plk3 assessed as casein substrate phosphorylation | B | 7.32 | pIC50 | 48 | nM | IC50 | J Biol Chem (2007) 282: 2505-2511 [PMID:17135248] |
phosphatidylinositol 4-kinase alpha in Human [GtoPdb: 2148] [UniProtKB: P42356] | ||||||||
GtoPdb | - | - | 6.8 | pIC50 | - | - | - |
J Biol Chem (1997) 272: 4384-90 [PMID:9020160]; Biochim Biophys Acta (1999) 1437: 341-56 [PMID:10101268] |
phosphatidylinositol 4-kinase beta in Human [GtoPdb: 2149] [UniProtKB: Q9UBF8] | ||||||||
GtoPdb | - | - | 6.8 | pIC50 | - | - | - |
J Biol Chem (1997) 272: 4384-90 [PMID:9020160]; Biochim Biophys Acta (1999) 1437: 341-56 [PMID:10101268] |
ChEMBL data shown on this page come from version 32:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]