ChEMBL ligand: CHEMBL1076555
5-LOX/Arachidonate 5-lipoxygenase in Human [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917] There should be some charts here, you may need to enable JavaScript!
Salivary alpha-amylase in Human [ChEMBL: CHEMBL2478] [UniProtKB: P0DUB6] There should be some charts here, you may need to enable JavaScript!
sphingosine kinase 1/Sphingosine kinase 1 in Human [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1] There should be some charts here, you may need to enable JavaScript!
sphingosine kinase 2/Sphingosine kinase 2 in Human [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0] sphingosine kinase 2/Sphingosine kinase 2 in Mouse [ChEMBL: CHEMBL1075305] [GtoPdb: 2205] [UniProtKB: Q9JIA7] There should be some charts here, you may need to enable JavaScript!
Transitional endoplasmic reticulum ATPase in Human [ChEMBL: CHEMBL1075145] [UniProtKB: P55072] There should be some charts here, you may need to enable JavaScript!
delta 4-desaturase, sphingolipid 1 in Human [GtoPdb: 2484] [UniProtKB: O15121] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
5-LOX/Arachidonate 5-lipoxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917]
ChEMBL	Inhibition of 5-lipoxygenase in human PMNL using arachidonic acid as substrate preincubated for 15 mins before substrate addition measured after 10 mins	B	6.15	pIC50	700	nM	IC50	Eur J Med Chem (2014) 84: 302-311 [PMID:25036790]
ChEMBL	Inhibition of partially purified recombinant 5-lipoxygenase (unknown origin) using arachidonic acid as substrate preincubated for 15 mins before substrate addition measured after 10 mins by HPLC analysis	B	7.52	pIC50	30	nM	IC50	Eur J Med Chem (2014) 84: 302-311 [PMID:25036790]
Salivary alpha-amylase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2478] [UniProtKB: P0DUB6]
ChEMBL	Inhibition of human salivary alpha-amylase using GalG2CNP as substrate by UV-Vis spectrophotometric analysis	B	2.98	pIC50	1043000	nM	IC50	Bioorg Med Chem (2015) 23: 6725-6732 [PMID:26395057]
sphingosine kinase 1/Sphingosine kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1]
ChEMBL	Inhibition of human recombinant SphK1 expressed in Sf9 cells assessed as radiolabeled products using 5 uM sphingosine and 10 uM gamma[32P]ATP by liquid scintillation counting	B	4.77	pKi	17000	nM	Ki	J Med Chem (2015) 58: 1879-1899 [PMID:25643074]
ChEMBL	Inhibition of recombinant human GST-tagged SphK1 using [3H]-sphingosine as substrate after 30 mins by scintillation counting method	B	4.77	pKi	17000	nM	Ki	Eur J Med Chem (2017) 139: 461-481 [PMID:28822281]
ChEMBL	Inhibition of SPHK1 (unknown origin)	B	4.77	pKi	17000	nM	Ki	Bioorg Med Chem (2016) 24: 3218-3230 [PMID:27255176]
ChEMBL	Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	4.8	pKi	16000	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]
ChEMBL	Inhibition of SK1 (unknown origin)	B	4.8	pKi	16000	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human SPHK1 expressed in Escherichia coli using [3-3H]sphingosine as substrate after 30 mins by scintillation counting	B	4.8	pKi	16000	nM	Ki	J Med Chem (2017) 60: 3933-3957 [PMID:28406646]
ChEMBL	Inhibition of full-length SK1 (unknown origin) (2 to 384 residues) expressed in Escherichia coli using Sph as substrate by resorufin based fluorescence assay	B	4.8	pKi	16000	nM	Ki	J Med Chem (2019) 62: 3658-3676 [PMID:30889352]
GtoPdb	-	-	4.8	pKi	16000	nM	Ki	J Pharmacol Exp Ther (2010) 333: 129-39 [PMID:20061445]
ChEMBL	Inhibition of SPHK1	B	4.92	pKi	12000	nM	Ki	J Med Chem (2011) 54: 3524-3548 [PMID:21495716]
ChEMBL	Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cells	B	4.92	pKi	12000	nM	Ki	J Med Chem (2011) 54: 3524-3548 [PMID:21495716]
ChEMBL	Inhibition of purified human SphK1 assessed as inhibition of formation of [33P]-S1P after 50 mins by scintillation counting	B	6.3	pKi	500	nM	Ki	Bioorg Med Chem Lett (2013) 23: 4608-4616 [PMID:23845219]
ChEMBL	Inhibition of recombinant human SphK1 using NBD-sphingosine as substrate after 2 hrs in presence of ATP by HPLC method	B	4.46	pIC50	35000	nM	IC50	Bioorg Med Chem (2017) 25: 3046-3052 [PMID:28408190]
ChEMBL	Inhibition of SK1 (unknown origin)	B	4.46	pIC50	35000	nM	IC50	J Med Chem (2018) 61: 9811-9840 [PMID:29969256]
GtoPdb	Measured using a GST-hSPHK1 fusion protein	-	6.3	pIC50	500	nM	IC50	Cancer Res (2003) 63: 5962-9 [PMID:14522923]
sphingosine kinase 2/Sphingosine kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0]
ChEMBL	Inhibition of human recombinant SphK2 expressed in Sf9 cells assessed as radiolabeled products using 10 uM sphingosine and 10 uM gamma[32P]ATP by liquid scintillation counting	B	4.3	pKi	50000	nM	Ki	J Med Chem (2015) 58: 1879-1899 [PMID:25643074]
ChEMBL	Inhibition of SPHK2 (unknown origin)	B	4.3	pKi	50000	nM	Ki	Bioorg Med Chem (2016) 24: 3218-3230 [PMID:27255176]
GtoPdb	-	-	5.1	pKi	7900	nM	Ki	PLoS ONE (2012) 7: e44543 [PMID:22970244]
ChEMBL	Inhibition of SK2 (unknown origin)	B	5.1	pKi	7900	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human SPHK2 expressed in Escherichia coli using [3-3H]sphingosine as substrate after 30 mins by scintillation counting	B	5.1	pKi	7900	nM	Ki	J Med Chem (2017) 60: 3933-3957 [PMID:28406646]
ChEMBL	Inhibition of recombinant human SphK2 assessed as decrease in [33P]SIP production using sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	5.1	pKi	7900	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]
ChEMBL	Inhibition of SK2 (unknown origin)	B	5.17	pKi	6700	nM	Ki	J Med Chem (2019) 62: 3658-3676 [PMID:30889352]
ChEMBL	Inhibition of recombinant human SphK2 using NBD-sphingosine as substrate after 2 hrs in presence of ATP by HPLC method	B	4.7	pIC50	20000	nM	IC50	Bioorg Med Chem (2017) 25: 3046-3052 [PMID:28408190]
ChEMBL	Inhibition of SK2 (unknown origin)	B	4.7	pIC50	20000	nM	IC50	J Med Chem (2018) 61: 9811-9840 [PMID:29969256]
sphingosine kinase 2/Sphingosine kinase 2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075305] [GtoPdb: 2205] [UniProtKB: Q9JIA7]
ChEMBL	Inhibition of mouse recombinant SPHK2 expressed in baculovirus-infected Sf9 cells	B	4.48	pKi	33000	nM	Ki	J Med Chem (2011) 54: 3524-3548 [PMID:21495716]
Transitional endoplasmic reticulum ATPase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075145] [UniProtKB: P55072]
ChEMBL	Inhibition of VCP assessed as conversion of ATP to ADP by enzyme coupled glucokinase assay	B	7.15	pIC50	70	nM	IC50	Bioorg Med Chem Lett (2010) 20: 1677-1679 [PMID:20137940]
ChEMBL	Inhibition of VCP in human HeLa cells assessed as stabilization of ubiquitin-tagged luciferase after 24 hrs by reporter gene assay	B	5.92	pEC50	<1200	nM	EC50	Bioorg Med Chem Lett (2010) 20: 1677-1679 [PMID:20137940]
delta 4-desaturase, sphingolipid 1 in Human [GtoPdb: 2484] [UniProtKB: O15121]
GtoPdb	-	-	6.52	pKi	300	nM	Ki	J Lipid Res (2014) 55: 1711-20 [PMID:24875537]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]