ChEMBL ligand: CHEMBL222102 (KU-55933)
protein kinase, DNA-activated, catalytic subunit/DNA-dependent protein kinase in Human [ChEMBL: CHEMBL3142] [GtoPdb: 2800] [UniProtKB: P78527] There should be some charts here, you may need to enable JavaScript!
histone deacetylase 6/Histone deacetylase 6 in Human [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7] There should be some charts here, you may need to enable JavaScript!
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha subunit in Human [ChEMBL: CHEMBL4005] [GtoPdb: 2153] [UniProtKB: P42336] There should be some charts here, you may need to enable JavaScript!
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3-kinase p110-beta subunit in Human [ChEMBL: CHEMBL3145] [GtoPdb: 2154] [UniProtKB: P42338] There should be some charts here, you may need to enable JavaScript!
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma/PI3-kinase p110-gamma subunit in Human [ChEMBL: CHEMBL3267] [GtoPdb: 2156] [UniProtKB: P48736] There should be some charts here, you may need to enable JavaScript!
ATM serine/threonine kinase/Serine-protein kinase ATM in Human [ChEMBL: CHEMBL3797] [GtoPdb: 1934] [UniProtKB: Q13315] There should be some charts here, you may need to enable JavaScript!
ATR serine/threonine kinase/Serine-protein kinase ATR in Human [ChEMBL: CHEMBL5024] [GtoPdb: 1935] [UniProtKB: Q13535] There should be some charts here, you may need to enable JavaScript!
mechanistic target of rapamycin kinase/Serine/threonine-protein kinase mTOR in Human [ChEMBL: CHEMBL2842] [GtoPdb: 2109] [UniProtKB: P42345] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
protein kinase, DNA-activated, catalytic subunit/DNA-dependent protein kinase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3142] [GtoPdb: 2800] [UniProtKB: P78527]
ChEMBL	Inhibition of DNAPK after 2 hrs by radiometric phosphate incorporation assay	B	5.6	pIC50	2500	nM	IC50	J Med Chem (2011) 54: 2320-2330 [PMID:21413798]
ChEMBL	Inhibition of DNA-PK in HeLa cell nuclear extracts	B	5.74	pIC50	1800	nM	IC50	J Med Chem (2007) 50: 1958-1972 [PMID:17371003]
ChEMBL	Inhibition of DNAPK (unknown origin) using p53-based peptide substrate preincubated for 5 min prior to ATP addition	B	6.74	pIC50	183	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
histone deacetylase 6/Histone deacetylase 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1865] [GtoPdb: 2618] [UniProtKB: Q9UBN7]
ChEMBL	Determination of IC50 values for inhibition of enzymatic assay of human HDAC6 with custom peptide substrate	B	5.32	pIC50	4798	nM	IC50	HDAC6 screening dataset using tau-based substrate in an enzymatic assay yields selective inhibitors and activators
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha/PI3-kinase p110-alpha subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4005] [GtoPdb: 2153] [UniProtKB: P42336]
ChEMBL	Inhibition of human recombinant PI3Kalpha using PIP2/ATP as substrate incubated for 20 mins followed by substrate addition by Kinase Glo reagent based luminescence assay	B	6.34	pIC50	458	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta/PI3-kinase p110-beta subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3145] [GtoPdb: 2154] [UniProtKB: P42338]
ChEMBL	Inhibition of human recombinant PI3Kbeta using PIP2/ATP as substrate incubated for 20 mins followed by substrate addition by Kinase Glo reagent based luminescence assay	B	6.88	pIC50	133	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma/PI3-kinase p110-gamma subunit in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3267] [GtoPdb: 2156] [UniProtKB: P48736]
ChEMBL	Inhibition of human recombinant PI3Kgamma using PIP2/ATP as substrate incubated for 20 mins followed by substrate addition by Kinase Glo reagent based luminescence assay	B	6	pIC50	>1000	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
ATM serine/threonine kinase/Serine-protein kinase ATM in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3797] [GtoPdb: 1934] [UniProtKB: Q13315]
ChEMBL	Inhibition of ATM	B	8.66	pKi	2.2	nM	Ki	Chem Biol (2005) 12: 621-637 [PMID:15975507]
GtoPdb	-	-	8.66	pKi	2.2	nM	Ki	Cancer Res (2004) 64: 9152-9 [PMID:15604286]
ChEMBL	Inhibition of ATM phosphorylation at Ser-1981 residue in human HT-29 cells incubated for 1 hr followed by X-ray irradiation by Hoechst staining based fluorescence plate reader analysis	B	5.91	pIC50	1220	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
ChEMBL	Inhibition of ATM kinase in human MCF7 cells after 1 hr by immunofluorescence assay	B	6.52	pIC50	300	nM	IC50	J Med Chem (2016) 59: 559-577 [PMID:26632965]
ChEMBL	Competitive inhibition of ATM (unknown origin) measured by ATP-competitive binding assay	B	6.6	pIC50	250	nM	IC50	Eur J Med Chem (2022) 233: 114196-114196 [PMID:35231830]
ChEMBL	Inhibition of ATM in human U2OS cells assessed as inhibition of p53 phosphorylation at Ser15 residue	B	6.6	pIC50	250	nM	IC50	J Med Chem (2016) 59: 559-577 [PMID:26632965]
ChEMBL	Inhibition of ATM isolated from human HeLa cell extract using glutathione S-transferase-p53N66 as substrate by ELISA	B	7.85	pIC50	14	nM	IC50	Bioorg Med Chem Lett (2012) 22: 5352-5359 [PMID:22835870]
ChEMBL	Inhibition of ATM (unknown origin) by ELISA method	B	7.89	pIC50	13	nM	IC50	J Med Chem (2023) 66: 9495-9518 [PMID:37438997]
ChEMBL	Inhibition of full length recombinant ATM after 24 hrs by radiometric phosphate incorporation assay	B	7.89	pIC50	13	nM	IC50	J Med Chem (2011) 54: 2320-2330 [PMID:21413798]
ChEMBL	Inhibition of human ATM using p53 as substrate preincubated for 10 mins by ELISA	B	7.89	pIC50	13	nM	IC50	J Med Chem (2016) 59: 559-577 [PMID:26632965]
ChEMBL	Inhibition of ATM (unknown origin) by biochemical assay	B	7.89	pIC50	13	nM	IC50	J Med Chem (2019) 62: 2988-3008 [PMID:30840447]
ChEMBL	Inhibition of ATM kinase	B	7.89	pIC50	13	nM	IC50	J Med Chem (2007) 50: 1958-1972 [PMID:17371003]
ChEMBL	Inhibition of human HeLa nuclear extract derived ATM using glutathioneS-transferase-p53N66 as substrate preincubated for 10 mins followed by ATP addition and measured after 1 hr by ELISA	B	7.89	pIC50	12.9	nM	IC50	J Med Chem (2020) 63: 14151-14183 [PMID:33135887]
GtoPdb	-	-	7.89	pIC50	13	nM	IC50	Cancer Res (2004) 64: 9152-9 [PMID:15604286]
ChEMBL	Inhibition of ATM (unknown origin) using p53 as substrate incubated for 30 mins followed by substrate addition measured after 2 hrs in presence of ATP by HTRF method	B	8.7	pIC50	<2	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
ATR serine/threonine kinase/Serine-protein kinase ATR in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5024] [GtoPdb: 1935] [UniProtKB: Q13535]
ChEMBL	Inhibition of full length recombinant ATR after 24 hrs by radiometric phosphate incorporation assay	B	4	pIC50	>100000	nM	IC50	J Med Chem (2011) 54: 2320-2330 [PMID:21413798]
ChEMBL	Inhibition of ATR in human HT-29 cells assessed as reduction in Chk1 phosphorylation at Ser-345 residue after 60 mins in presence of 4-nitroquinoline-1-oxide by Hoechst 33258 staining based plate reader analysis	B	4.52	pIC50	>30000	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]
mechanistic target of rapamycin kinase/Serine/threonine-protein kinase mTOR in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2842] [GtoPdb: 2109] [UniProtKB: P42345]
ChEMBL	Inhibition of mTOR by radiometric phosphate incorporation assay	B	5.03	pIC50	9300	nM	IC50	J Med Chem (2011) 54: 2320-2330 [PMID:21413798]
ChEMBL	Inhibition of C-terminal FLAG-tagged recombinant mTOR (1362 to 2549 residues) (unknown origin) using Biotin-Ahx-Lys-Lys-Ala-Asn-Gln-Val-Phe-Leu-Gly-Phe-Thr-Tyr-Val-Ala-Pro-Ser-Val-Leu-Glu-Ser-Val-Lys-Glu-NH2 as substrate after 90 mins by AlphaScreen assay	B	5.25	pIC50	5580	nM	IC50	J Med Chem (2016) 59: 6281-6292 [PMID:27259031]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]