ChEMBL ligand: CHEMBL559147
C-C motif chemokine 2 in Human [ChEMBL: CHEMBL1649052] [UniProtKB: P13500] There should be some charts here, you may need to enable JavaScript!
Huntingtin in Human [ChEMBL: CHEMBL5514] [UniProtKB: P42858] There should be some charts here, you may need to enable JavaScript!
leucine rich repeat kinase 2/Leucine-rich repeat serine/threonine-protein kinase 2 in Human [ChEMBL: CHEMBL1075104] [GtoPdb: 2059] [UniProtKB: Q5S007] There should be some charts here, you may need to enable JavaScript!
Rho associated coiled-coil containing protein kinase 1/Rho-associated protein kinase 1 in Human [ChEMBL: CHEMBL3231] [GtoPdb: 1503] [UniProtKB: Q13464] There should be some charts here, you may need to enable JavaScript!
Rho associated coiled-coil containing protein kinase 2/Rho-associated protein kinase 2 in Human [ChEMBL: CHEMBL2973] [GtoPdb: 1504] [UniProtKB: O75116] Rho associated coiled-coil containing protein kinase 2/Rho-associated protein kinase 2 in Rat [ChEMBL: CHEMBL5490] [GtoPdb: 1504] [UniProtKB: Q62868] There should be some charts here, you may need to enable JavaScript!
ribosomal protein S6 kinase A5/Ribosomal protein S6 kinase alpha-5 in Human [ChEMBL: CHEMBL4237] [GtoPdb: 1523] [UniProtKB: O75582] There should be some charts here, you may need to enable JavaScript!
protein kinase N2/Serine/threonine-protein kinase N2 in Human [ChEMBL: CHEMBL3032] [GtoPdb: 1521] [UniProtKB: Q16513] There should be some charts here, you may need to enable JavaScript!
Kv11.1/Voltage-gated inwardly rectifying potassium channel KCNH2 in Human [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809] There should be some charts here, you may need to enable JavaScript!
protein kinase C epsilon in Human [GtoPdb: 1486] [UniProtKB: Q02156] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
C-C motif chemokine 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1649052] [UniProtKB: P13500]
ChEMBL	Inhibition of MCP1-mediated human THP cell migration	B	5.58	pIC50	2630.27	nM	IC50	Bioorg Med Chem Lett (2011) 21: 97-101 [PMID:21145740]
Huntingtin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5514] [UniProtKB: P42858]
ChEMBL	Inhibition of HTT (unknown origin)	B	5.3	pIC50	5000	nM	IC50	J Med Chem (2022) 65: 15993-16032 [PMID:36490325]
leucine rich repeat kinase 2/Leucine-rich repeat serine/threonine-protein kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075104] [GtoPdb: 2059] [UniProtKB: Q5S007]
ChEMBL	Inhibition of GST-tagged LRRK2 G2019S/A2016T mutant (1326 to 2527 residues) (unknown origin) in presence of ATP	B	4.89	pIC50	13000	nM	IC50	Biochem J (2009) 424: 47-60 [PMID:19740074]
ChEMBL	Inhibition of GST-tagged LRRK2 (1326 to 2527 residues) (unknown origin) using peptide as substrate incubated for 15 mins in presence of ATP by immunoblotting analysis	B	5.64	pIC50	2300	nM	IC50	Biochem J (2009) 424: 47-60 [PMID:19740074]
ChEMBL	Inhibition of GST-tagged LRRK2 G2019S mutant (1326 to 2527 residues) (unknown origin) using peptide as substrate incubated for 15 mins in presence of ATP by immunoblotting analysis	B	6	pIC50	1000	nM	IC50	Biochem J (2009) 424: 47-60 [PMID:19740074]
ChEMBL	Inhibition of GST-tagged LRRK2 G2019S mutant (1326 to 2527 residues) (unknown origin) in presence of ATP	B	6	pIC50	1000	nM	IC50	Biochem J (2009) 424: 47-60 [PMID:19740074]
Rho associated coiled-coil containing protein kinase 1/Rho-associated protein kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3231] [GtoPdb: 1503] [UniProtKB: Q13464]
ChEMBL	Inhibition of ROCK1 (unknown origin)	B	6.66	pKi	220	nM	Ki	Medchemcomm (2012) 3: 699-709 [PMID:23275831]
ChEMBL	Inhibition of ROCK1 (unknown origin)	B	6.66	pKi	220	nM	Ki	Eur J Med Chem (2023) 250: 115181-115181 [PMID:36764122]
ChEMBL	Inhibition of human leukocytic ROCK1 expressed in insect cells using KKRNRTLSV as substrate after 10 mins by pyruvate kinase/lactate dehydrogenase coupled assay	B	6.82	pKi	150	nM	Ki	J Med Chem (2015) 58: 5028-5037 [PMID:26039570]
ChEMBL	Inhibitory constant against ROCK1	B	6.82	pKi	150	nM	Ki	J Biol Chem (2006) 281: 260-268 [PMID:16249185]
ChEMBL	Inhibition of human ROCK1 by homogenous luciferase assay	B	6.82	pKi	150	nM	Ki	J Med Chem (2010) 53: 759-777 [PMID:20000469]
GtoPdb	-	-	6.85	pKi	140	nM	Ki	Nature (1997) 389: 990-4 [PMID:9353125]
ChEMBL	Inhibition of ROCK1 (unknown origin)	B	6.85	pKi	140	nM	Ki	J Med Chem (2021) 64: 1283-1345 [PMID:33481605]
ChEMBL	ROCK1 and ROCK2 Compound Selectivity: Dose response curves for Rho-kinase inhibition were derived from a Millipore immuno-based 96 well plate assay (Millipore catalog number CSA001). Purified active ROCK1 and ROCK2 were obtained from Invitrogen (catalog numbers ROCK1, PV3691 and ROCK2, PV3759). The kit components include assay plates, which are pre-coated with recombinant MYPT1, which contains a specifically phosphorylatable Thr696. The inhibitory activities of compounds are measured according to the manufactures protocol. Briefly, decreasing concentrations of test compounds or the known ROCK inhibitor Y-27963, are added, from 50 uM to 0.003 uM to reaction buffer containing 5 mM MgCl2, and 10 mUnits of ROCK1 or ROCK2 in assay dilution buffer. This mixture is overlayed into the 96 well plate and the reaction is initiated with the addition of 2.5 uM ATP. The assay proceeds at 30° Celsius for 30 minutes with gentle shaking at 120 rpm. The assay is terminated by washing of the plate 3 times with Tris-buffered saline and tween wash buffer. Anti-phospho-MYPT1 (Thr696) antibody is added to each well to detect the phosphorylated substrate and incubated for 1 hour at room temperature after which HRP conjugated anti-rabbit IgG secondary is added for 1 hour at room temperature. After washing the assay is developed using a substrate reagent and the absorbance is read at 450 nm on a Tecan Infinite M1000 reflecting the relative remaining ROCK phosphorylation activity.	B	7.4	pKi	40	nM	Ki	US-10183931-B2. Rho kinase inhibitors (2019)
ChEMBL	Inhibition of ROCK-1	B	7.6	pKi	25.12	nM	Ki	Bioorg Med Chem Lett (2011) 21: 97-101 [PMID:21145740]
ChEMBL	Inhibition of N terminal histidine tagged human recombinant ROCK1 (3 to 543 residues) in HASMC cells incubated for 2 hrs by ELISA method	B	4.52	pIC50	30000	nM	IC50	J Med Chem (2021) 64: 1786-1815 [PMID:33569941]
ChEMBL	Inhibition of N terminal histidine tagged human recombinant ROCK1 (3 to 543 residues) expressed in baculovirus infected Sf9 cells using biotin-Ahx-AKRRRLSSLRA-CONH2 as a substrate incubated for 10 mins by scintillation counting method	B	5.85	pIC50	1400	nM	IC50	J Med Chem (2021) 64: 1786-1815 [PMID:33569941]
ChEMBL	ROCK1 and ROCK2 Compound Selectivity: Dose response curves for Rho-kinase inhibition were derived from a Millipore immuno-based 96 well plate assay (Millipore catalog number CSA001). Purified active ROCK1 and ROCK2 were obtained from Invitrogen (catalog numbers ROCK1, PV3691 and ROCK2, PV3759). The kit components include assay plates, which are pre-coated with recombinant MYPT1, which contains a specifically phosphorylatable Thr696. The inhibitory activities of compounds are measured according to the manufactures protocol. Briefly, decreasing concentrations of test compounds or the known ROCK inhibitor Y-27963, are added, from 50 uM to 0.003 uM to reaction buffer containing 5 mM MgCl2, and 10 mUnits of ROCK1 or ROCK2 in assay dilution buffer. This mixture is overlayed into the 96 well plate and the reaction is initiated with the addition of 2.5 uM ATP. The assay proceeds at 30° Celsius for 30 minutes with gentle shaking at 120 rpm. The assay is terminated by washing of the plate 3 times with Tris-buffered saline and tween wash buffer. Anti-phospho-MYPT1 (Thr696) antibody is added to each well to detect the phosphorylated substrate and incubated for 1 hour at room temperature after which HRP conjugated anti-rabbit IgG secondary is added for 1 hour at room temperature. After washing the assay is developed using a substrate reagent and the absorbance is read at 450 nm on a Tecan Infinite M1000 reflecting the relative remaining ROCK phosphorylation activity.	B	5.95	pIC50	1130	nM	IC50	US-10183931-B2. Rho kinase inhibitors (2019)
ChEMBL	Inhibition of ROCK-1 by Immobilized metal ion affinity-based fluorescence polarization assay	B	6.06	pIC50	870.96	nM	IC50	Bioorg Med Chem Lett (2011) 21: 97-101 [PMID:21145740]
ChEMBL	Inhibition of ROCK1 (unknown origin)	B	6.7	pIC50	200	nM	IC50	J Med Chem (2016) 59: 788-809 [PMID:26356532]
ChEMBL	Inhibition of human recombinant N-terminal his-tagged ROCK1 (3-543) expressed in baculovirus infected Sf9 cells using Biotin-Ahx-AKRRLSSLRA-CONH2 substrate and [gamma-33P]ATP after 90 mins by scintillation proximity assay	B	6.82	pIC50	150	nM	IC50	J Pharmacol Exp Ther (2007) 320: 89-98 [PMID:17018693]
ChEMBL	Inhibition of ROCK-1 (unknown origin)	B	7.03	pIC50	93	nM	IC50	Bioorg Med Chem Lett (2014) 24: 4812-4817 [PMID:25248678]
GtoPdb	-	-	7.34	pIC50	46	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3235-9 [PMID:20462760]; Hypertension (2009) 54: 676-83 [PMID:19597037]
Rho associated coiled-coil containing protein kinase 2/Rho-associated protein kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2973] [GtoPdb: 1504] [UniProtKB: O75116]
ChEMBL	Inhibition of human ROCK2 by homogenous luciferase assay	B	6.52	pKi	300	nM	Ki	J Med Chem (2010) 53: 759-777 [PMID:20000469]
ChEMBL	Inhibition of ROCK2 (unknown origin)	B	6.52	pKi	300	nM	Ki	Medchemcomm (2012) 3: 699-709 [PMID:23275831]
ChEMBL	Inhibition of ROCK2 (unknown origin)	B	6.52	pKi	300	nM	Ki	J Med Chem (2021) 64: 1283-1345 [PMID:33481605]
ChEMBL	Inhibition of human ROCK2 (unknown origin)	B	6.52	pKi	300	nM	Ki	Eur J Med Chem (2023) 250: 115181-115181 [PMID:36764122]
ChEMBL	Inhibition of ROCK2 (unknown origin) assessed as inhibition constant	B	6.85	pKi	140	nM	Ki	J Med Chem (2016) 59: 2269-2300 [PMID:26486225]
GtoPdb	-	-	6.94	pKi	114	nM	Ki	Nature (1997) 389: 990-4 [PMID:9353125]; Hypertension (2009) 54: 676-83 [PMID:19597037]
ChEMBL	ROCK1 and ROCK2 Compound Selectivity: Dose response curves for Rho-kinase inhibition were derived from a Millipore immuno-based 96 well plate assay (Millipore catalog number CSA001). Purified active ROCK1 and ROCK2 were obtained from Invitrogen (catalog numbers ROCK1, PV3691 and ROCK2, PV3759). The kit components include assay plates, which are pre-coated with recombinant MYPT1, which contains a specifically phosphorylatable Thr696. The inhibitory activities of compounds are measured according to the manufactures protocol. Briefly, decreasing concentrations of test compounds or the known ROCK inhibitor Y-27963, are added, from 50 uM to 0.003 uM to reaction buffer containing 5 mM MgCl2, and 10 mUnits of ROCK1 or ROCK2 in assay dilution buffer. This mixture is overlayed into the 96 well plate and the reaction is initiated with the addition of 2.5 uM ATP. The assay proceeds at 30° Celsius for 30 minutes with gentle shaking at 120 rpm. The assay is terminated by washing of the plate 3 times with Tris-buffered saline and tween wash buffer. Anti-phospho-MYPT1 (Thr696) antibody is added to each well to detect the phosphorylated substrate and incubated for 1 hour at room temperature after which HRP conjugated anti-rabbit IgG secondary is added for 1 hour at room temperature. After washing the assay is developed using a substrate reagent and the absorbance is read at 450 nm on a Tecan Infinite M1000 reflecting the relative remaining ROCK phosphorylation activity.	B	7.22	pKi	60	nM	Ki	US-10183931-B2. Rho kinase inhibitors (2019)
ChEMBL	ROCK1 and ROCK2 Compound Selectivity: Dose response curves for Rho-kinase inhibition were derived from a Millipore immuno-based 96 well plate assay (Millipore catalog number CSA001). Purified active ROCK1 and ROCK2 were obtained from Invitrogen (catalog numbers ROCK1, PV3691 and ROCK2, PV3759). The kit components include assay plates, which are pre-coated with recombinant MYPT1, which contains a specifically phosphorylatable Thr696. The inhibitory activities of compounds are measured according to the manufactures protocol. Briefly, decreasing concentrations of test compounds or the known ROCK inhibitor Y-27963, are added, from 50 uM to 0.003 uM to reaction buffer containing 5 mM MgCl2, and 10 mUnits of ROCK1 or ROCK2 in assay dilution buffer. This mixture is overlayed into the 96 well plate and the reaction is initiated with the addition of 2.5 uM ATP. The assay proceeds at 30° Celsius for 30 minutes with gentle shaking at 120 rpm. The assay is terminated by washing of the plate 3 times with Tris-buffered saline and tween wash buffer. Anti-phospho-MYPT1 (Thr696) antibody is added to each well to detect the phosphorylated substrate and incubated for 1 hour at room temperature after which HRP conjugated anti-rabbit IgG secondary is added for 1 hour at room temperature. After washing the assay is developed using a substrate reagent and the absorbance is read at 450 nm on a Tecan Infinite M1000 reflecting the relative remaining ROCK phosphorylation activity.	B	5.79	pIC50	1630	nM	IC50	US-10183931-B2. Rho kinase inhibitors (2019)
ChEMBL	Inhibition of Protein kinase ROCK2 (ROCKII)	B	6.15	pIC50	700	nM	IC50	J Med Chem (2003) 46: 1478-1483 [PMID:12672248]
ChEMBL	Inhibition of His6-tagged ROCK2 (2 to 543 residues) (unknown origin) using peptide as substrate incubated for 15 mins in presence of ATP by immunoblotting analysis	B	6.15	pIC50	700	nM	IC50	Biochem J (2009) 424: 47-60 [PMID:19740074]
ChEMBL	Inhibition of human ROCK2 by homogenous luciferase assay	B	6.59	pIC50	260	nM	IC50	J Med Chem (2010) 53: 759-777 [PMID:20000469]
ChEMBL	Inhibition of ROCK-2 by Immobilized metal ion affinity-based fluorescence polarization assay	B	6.61	pIC50	245.47	nM	IC50	Bioorg Med Chem Lett (2011) 21: 97-101 [PMID:21145740]
ChEMBL	Inhibition of recombinant N-terminal GST-tagged human ROCK2 (5 to 554 residues) expressed in baculovirus infected Sf9 cells using S6K as substrate incubated for 30 mins in presence of ATP by ADP Glo kinase assay	B	6.84	pIC50	143	nM	IC50	J Med Chem (2022) 65: 9819-9845 [PMID:35816678]
ChEMBL	Inhibition of ROCK2 (unknown origin)	B	6.85	pIC50	140	nM	IC50	J Med Chem (2016) 59: 788-809 [PMID:26356532]
ChEMBL	Inhibition of human recombinant ROCK-2 using KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK as substrate after 40 mins by scintillation counting analysis	B	7.05	pIC50	89	nM	IC50	Bioorg Med Chem Lett (2014) 24: 4812-4817 [PMID:25248678]
GtoPdb	-	-	7.21	pIC50	61	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3235-9 [PMID:20462760]; Hypertension (2009) 54: 676-83 [PMID:19597037]
ChEMBL	Inhibition of ROCK2 (unknown origin) using long S6 kinase peptide as substrate by radiometric assay	B	7.27	pIC50	54	nM	IC50	Bioorg Med Chem Lett (2013) 23: 6442-6446 [PMID:24119557]
ChEMBL	Inhibition of human N-terminal GST-tagged ROCK2 expressed in baculovirus-infected insect cells using long S6 kinase peptide as substrate by radiometric assay in presence of [32P]ATP	B	7.27	pIC50	54	nM	IC50	J Med Chem (2015) 58: 4309-4324 [PMID:25898023]
ChEMBL	Inhibition of ROCK2 (unknown origin) assessed as phosphorylation of MYPT1 using Tetramethylbenzidine as substrate in presence of ATP	B	8.08	pIC50	8.3	nM	IC50	ACS Med Chem Lett (2020) 11: 1694-1703 [PMID:32944136]
Rho associated coiled-coil containing protein kinase 2/Rho-associated protein kinase 2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5490] [GtoPdb: 1504] [UniProtKB: Q62868]
ChEMBL	Inhibition of rat ROCK2 expressed in Sf9 cells	B	6.1	pIC50	800	nM	IC50	Biochem J (2000) 351: 95-105 [PMID:10998351]
ribosomal protein S6 kinase A5/Ribosomal protein S6 kinase alpha-5 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4237] [GtoPdb: 1523] [UniProtKB: O75582]
ChEMBL	Inhibition of His-tagged human MSK1 expressed in Sf9 cells	B	5.08	pIC50	8300	nM	IC50	Biochem J (2000) 351: 95-105 [PMID:10998351]
protein kinase N2/Serine/threonine-protein kinase N2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3032] [GtoPdb: 1521] [UniProtKB: Q16513]
ChEMBL	Inhibition of Protein kinase C related kinase 2 (PRK2)	B	6.22	pIC50	600	nM	IC50	J Med Chem (2003) 46: 1478-1483 [PMID:12672248]
ChEMBL	Inhibition of His-tagged human PRK2 expressed in HEK293 cells	B	6.22	pIC50	600	nM	IC50	Biochem J (2000) 351: 95-105 [PMID:10998351]
GtoPdb	-	-	7.15	pIC50	70	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3235-9 [PMID:20462760]
Kv11.1/Voltage-gated inwardly rectifying potassium channel KCNH2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL240] [GtoPdb: 572] [UniProtKB: Q12809]
ChEMBL	Displacement of [3H]dofetilide from human ERG	B	4.1	pKi	79432.82	nM	Ki	Bioorg Med Chem Lett (2011) 21: 97-101 [PMID:21145740]
protein kinase C epsilon in Human [GtoPdb: 1486] [UniProtKB: Q02156]
GtoPdb	-	-	6.47	pIC50	335	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3235-9 [PMID:20462760]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]