ChEMBL ligand: CHEMBL1444 (CGS 20267, CGS-20267, Femara, Letrozol, Letrozole, NSC-759652)
CYP11B1/Cytochrome P450 11B1 in Human [ChEMBL: CHEMBL1908] [GtoPdb: 1359] [UniProtKB: P15538] There should be some charts here, you may need to enable JavaScript!
CYP11B2/Cytochrome P450 11B2 in Human [ChEMBL: CHEMBL2722] [GtoPdb: 1360] [UniProtKB: P19099] There should be some charts here, you may need to enable JavaScript!
CYP19A1/Cytochrome P450 19A1 in Human [ChEMBL: CHEMBL1978] [GtoPdb: 1362] [UniProtKB: P11511] There should be some charts here, you may need to enable JavaScript!
Estrogen receptor-α/Estrogen receptor alpha in Human [ChEMBL: CHEMBL206] [GtoPdb: 620] [UniProtKB: P03372] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
CYP11B1/Cytochrome P450 11B1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1908] [GtoPdb: 1359] [UniProtKB: P15538]
ChEMBL	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxy-corticosterone as substrate	B	5.58	pIC50	2620	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
CYP11B2/Cytochrome P450 11B2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2722] [GtoPdb: 1360] [UniProtKB: P19099]
ChEMBL	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxy-corticosterone as substrate	B	5.85	pIC50	1420	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
CYP19A1/Cytochrome P450 19A1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1978] [GtoPdb: 1362] [UniProtKB: P11511]
ChEMBL	Inhibition of human aromatase-mediated conversion of [1beta3H]androstenedione to estrone by Lineweaver-Burke plot in presence of NADPH	B	8.66	pKi	2.2	nM	Ki	J Med Chem (2009) 52: 143-150 [PMID:19072235]
ChEMBL	Competitive inhibition of human aromatase using dibenzylfluorescein substrate after 10 mins preincubation measured every 10 sec for 5 mins by Michaelis-Menten and Dixon plot analysis	B	10.7	pKi	0.02	nM	Ki	Bioorg Med Chem (2012) 20: 2427-2434 [PMID:22386564]
GtoPdb	-	-	10.7	pKi	0.02	nM	Ki	Bioorg Med Chem (2012) 20: 2427-34 [PMID:22386564]
ChEMBL	Inhibition of aromatase activity in human T47D cells after 24 hrs	B	4.53	pIC50	29500	nM	IC50	Eur J Med Chem (2016) 124: 946-958 [PMID:27770735]
ChEMBL	Inhibition of aromatase activity in human T47D cells after 24 hrs	B	4.53	pIC50	29500	nM	IC50	Eur J Med Chem (2017) 134: 304-315 [PMID:28427017]
ChEMBL	Inhibition of human placental microsome CYP19	B	7.28	pIC50	52.48	nM	IC50	J Med Chem (2005) 48: 7282-7289 [PMID:16279787]
ChEMBL	Inhibition of human aromatase using androstenedione as substrate and NADPH preincubated for 24 hrs followed by substrate addition measured after 24 hrs by ELISA	B	7.31	pIC50	49.5	nM	IC50	Eur J Med Chem (2018) 143: 426-437 [PMID:29202405]
ChEMBL	Inhibition of CYP19 isolated from microsomal fraction of human term placental tissue using [1beta-3H] androstenedione as substrate preincubated for 5 mins followed by enzyme addition measured after 20 mins by beta scintillation counting analysis	B	7.44	pIC50	36.2	nM	IC50	Medchemcomm (2012) 3: 663-666
ChEMBL	Inhibition of human CYP19 using [1beta-3H]androstenedione as substrate by 3H2O method	B	7.44	pIC50	36	nM	IC50	J Med Chem (2013) 56: 460-470 [PMID:23281812]
ChEMBL	Inhibition of aromatase (unknown origin)	B	7.59	pIC50	26	nM	IC50	Eur J Med Chem (2022) 229: 114097-114097 [PMID:34998057]
ChEMBL	Inhibition of aromatase in human placental microsomes assessed as inhibition of aromatization of [1,2,6,7-3H] androstenedione by flow scintillation analysis	B	7.74	pIC50	18	nM	IC50	Eur J Med Chem (2011) 46: 2541-2545 [PMID:21497425]
ChEMBL	Inhibition of human placental microsome aromatase after 30 mins by ELISA	B	7.78	pIC50	16.53	nM	IC50	Bioorg Med Chem Lett (2013) 23: 1760-1762 [PMID:23403081]
GtoPdb	-	-	7.9	pIC50	-	-	-	J Steroid Biochem Mol Biol (1990) 37: 1021-7 [PMID:2149502]
ChEMBL	Inhibition of human placental microsome CYP19	B	7.94	pIC50	11.5	nM	IC50	Bioorg Med Chem Lett (2010) 20: 3050-3064 [PMID:20413308]
ChEMBL	Inhibition of aromatase in human placental microsomes	B	7.94	pIC50	11.5	nM	IC50	Bioorg Med Chem Lett (2008) 18: 4713-4715 [PMID:18640836]
ChEMBL	Inhibition of recombinant human aromatase preincubated for 10 mins followed by substrate and beta-NADP+ addition and measured for 60 mins by fluorescence method	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2019) 168: 253-262 [PMID:30822713]
ChEMBL	Inhibition of human placental aromatase using androstenedione as substrate incubated in dark for 16 hrs	B	8	pIC50	10	nM	IC50	Eur J Med Chem (2022) 244: 114802-114802 [PMID:36240547]
ChEMBL	Inhibition of human aromatase using ASD as substrate incubated for 16 hrs by UV/vis-spectrophotometry	B	8	pIC50	10	nM	IC50	ACS Med Chem Lett (2020) 11: 732-739 [PMID:32435378]
ChEMBL	Inhibition of CYP19A1 (unknown origin) preincubated with NADPH followed by DBF substrate addition after 30 mins by fluorescence based assay	B	8	pIC50	9.95	nM	IC50	Bioorg Med Chem Lett (2017) 27: 5393-5399 [PMID:29153737]
ChEMBL	Inhibition of human placental aromatase using [3H]-1beta-androstenedione as substrate after 16 hrs by [3H]-water method	B	8	pIC50	9.9	nM	IC50	J Med Chem (2012) 55: 8464-8476 [PMID:22951074]
ChEMBL	Inhibition of aromatase using 7-methoxy-4-trifluoromethyl coumarin as substrate after 30 mins by fluorescence-based colorimetric analysis	B	8.1	pIC50	8	nM	IC50	Eur J Med Chem (2011) 46: 4010-4024 [PMID:21703734]
ChEMBL	Inhibition of human aromatase-mediated conversion of [1beta3H]androstenedione to estrone by liquid scintillation counting in presence of NADPH	B	8.21	pIC50	6.1	nM	IC50	J Med Chem (2009) 52: 143-150 [PMID:19072235]
ChEMBL	Inhibition of recombinant human CYP19 expressed in baculovirus infected insect cells using MFC as substrate measured after 30 mins by fluorometric analysis	B	8.28	pIC50	5.3	nM	IC50	Bioorg Med Chem (2016) 24: 5400-5409 [PMID:27647367]
ChEMBL	Inhibition of human recombinant aromatase using 7-methoxy-trifluoromethylcoumarin as substrate assessed as formation of fluorescent metabolite after 30 mins by fluorescence assay	B	8.28	pIC50	5.3	nM	IC50	J Med Chem (2015) 58: 2623-2648 [PMID:25751283]
ChEMBL	Inhibition of human recombinant aromatase using 7-methoxy-4-trifluoromethyl coumarin as substrate by fluorimetric analysis	B	8.38	pIC50	4.2	nM	IC50	J Med Chem (2011) 54: 4006-4017 [PMID:21604760]
ChEMBL	Inhibition of recombinant human aromatase using 7-methoxy-4-trifluoromethyl coumarin as substrate measured at anoxic conditions by fluorescence analysis	B	8.4	pIC50	4	nM	IC50	Bioorg Med Chem Lett (2016) 26: 3192-3194 [PMID:27161804]
ChEMBL	Inhibition of human aromatase assessed as reduction in fluorescence intensity preincubated with NADPH regenerating system for 10 mins followed by substrate addition incubated for 60 mins by fluorescence based microplate reader analysis	B	8.4	pIC50	4	nM	IC50	Eur J Med Chem (2021) 224: 113733-113733 [PMID:34364162]
ChEMBL	Inhibition of aromatase (unknown origin) using 7-methoxy-4-trifluoromethylcoumarin as substrate after 30 mins by fluorimetric analysis	B	8.47	pIC50	3.4	nM	IC50	J Med Chem (2013) 56: 7536-7551 [PMID:24025069]
ChEMBL	Inhibition of CYP19 (unknown origin) using O-benzyl fluorescein benzyl ester substrate preincubated for 10 mins by fluorimetric analysis relative to control	B	8.48	pIC50	3.3	nM	IC50	Bioorg Med Chem (2015) 23: 3472-3480 [PMID:25934226]
ChEMBL	Inhibition of human recombinant aromatase expressed in baculovirus insect cell using 7-methoxy-trifluoromethyl coumarin (MFC) as substrate preincubated for 10 mins followed by substrate addition and measured for 30 mins by fluorescence based assay	B	8.55	pIC50	2.8	nM	IC50	Eur J Med Chem (2021) 221: 113511-113511 [PMID:34000484]
ChEMBL	Inhibition of human recombinant aromatase expressed in baculovirus-infected cell system using 7-methoxy-trifluoromethylcoumarin as substrate after 30 mins by fluorescence assay	B	8.55	pIC50	2.8	nM	IC50	Bioorg Med Chem (2014) 22: 126-134 [PMID:24345481]
ChEMBL	Inhibition of recombinant human aromatase	B	8.55	pIC50	2.8	nM	IC50	Eur J Med Chem (2020) 186: 111893-111893 [PMID:31761383]
ChEMBL	Inhibition of recombinant human aromatase using 7-methoxy-trifluoromethylcoumarin as substrate incubated for 30 mins by fluorescence microplate reader analysis	B	8.66	pIC50	2.18	nM	IC50	Bioorg Med Chem (2016) 24: 4723-4730 [PMID:27567077]
ChEMBL	Inhibition of recombinant human aromatase using 7-methoxy-trifluoromethylcoumarin as substrate incubated for 30 mins by fluorescence microplate reader analysis	B	8.66	pIC50	2.18	nM	IC50	Bioorg Med Chem (2016) 24: 4723-4730 [PMID:27567077]
ChEMBL	Inhibition of aromatase in human breast tumor	B	8.7	pIC50	2	nM	IC50	Eur J Med Chem (2022) 241: 114658-114658 [PMID:35964426]
ChEMBL	Inhibition of human aromatase using dibenzylfluorescein substrate preincubated for 30 mins measured after 30 mins by fluorescence assay	B	8.7	pIC50	2	nM	IC50	Bioorg Med Chem (2012) 20: 2427-2434 [PMID:22386564]
ChEMBL	Inhibition of aromatase (unknown origin) using O-benzyl fluorescein benzyl ester as substrate in presence of NADPH-generating system by fluorescence assay	B	8.72	pIC50	1.9	nM	IC50	Eur J Med Chem (2018) 143: 1604-1615 [PMID:29137864]
ChEMBL	Inhibition of recombinant human aromatase expressed in baculovirus infected insect cells using O-benzyl fluorescein benzyl ester as substrate in presence of NADPH generating system by fluorescence based analysis	B	8.72	pIC50	1.9	nM	IC50	Bioorg Med Chem (2019) 27: 115040-115040 [PMID:31416738]
ChEMBL	Inhibition of aromatase in human MCF-7aro cells using [1beta-3H] androstenedione as substrate incubated for 1 hr by liquid scintillation counting method	B	8.72	pIC50	1.9	nM	IC50	Eur J Med Chem (2020) 185: 111815-111815 [PMID:31732252]
ChEMBL	Inhibition of human recombinant aromatase expressed in baculovirus infected insect cells using O-benzylfluorescein benzyl ester as substrate after 30 mins	B	8.96	pIC50	1.1	nM	IC50	Medchemcomm (2013) 4: 1590-1596
ChEMBL	Inhibition of human aromatase coexpressed with P450 reductase by fluorimetry	B	9	pIC50	1	nM	IC50	Bioorg Med Chem Lett (2012) 22: 1860-1863 [PMID:22335894]
ChEMBL	Inhibition of aromatase (unknown origin)	B	9	pIC50	1	nM	IC50	J Nat Prod (2021) 84: 1738-1747 [PMID:34110821]
ChEMBL	Inhibition of aromatase in human JEG3 cells by scintillation spectrometry	B	9.05	pIC50	0.89	nM	IC50	J Med Chem (2010) 53: 2155-2170 [PMID:20148564]
ChEMBL	Inhibition of aromatase activity in human JEG3 cells	B	9.05	pIC50	0.89	nM	IC50	J Med Chem (2008) 51: 4226-4238 [PMID:18590272]
ChEMBL	Inhibition of aromatase (unknown origin) expressed in JEG-3 cells	B	9.05	pIC50	0.89	nM	IC50	J Med Chem (2015) 58: 7634-7658 [PMID:25992880]
ChEMBL	Inhibition of aromatase in human JEG-3 cells using Androst-4-ene-3,17-dione as substrate incubated for 1 hr and measured by BCA assay	B	9.15	pIC50	0.7	nM	IC50	Eur J Med Chem (2022) 240: 114569-114569 [PMID:35834906]
ChEMBL	Inhibition of aromatase in human JEG-3 cells using Androst-4-ene-3,17-dione [1beta-3H] as substrate incubated for 1 hrs by scintillation counting analysis	B	9.15	pIC50	0.7	nM	IC50	RSC Med Chem (2023) 14: 356-366 [PMID:36846364]
Estrogen receptor-α/Estrogen receptor alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL206] [GtoPdb: 620] [UniProtKB: P03372]
ChEMBL	Binding affinity to human ERalpha measured after 2 hrs by fluorescence polarization plate reader	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2021) 224: 113733-113733 [PMID:34364162]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]