ChEMBL ligand: CHEMBL247378 (7-Nitroindazole)
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric oxide synthase, endothelial in Rat [ChEMBL: CHEMBL1075230] [GtoPdb: 1249] [UniProtKB: Q62600] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
GtoPdb	-	-	5.3	pIC50	5000	nM	IC50	Br J Pharmacol (1993) 110: 225-8 [PMID:7693279]
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase (nNOS)	B	5.54	pIC50	2870	nM	IC50	Bioorg Med Chem Lett (1999) 9: 2569-2572 [PMID:10498210]
ChEMBL	In vitro inhibition of human neuronal nitric oxide synthase	B	5.6	pIC50	2500	nM	IC50	J Med Chem (2004) 47: 1575-1586 [PMID:14998342]
ChEMBL	Inhibition of NOS1	B	6.15	pIC50	710	nM	IC50	Bioorg Med Chem (2009) 17: 6180-6187 [PMID:19679481]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of rat recombinant nNOS expressed in Saccharomyces cerevisiae assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay	B	4.57	pIC50	27000	nM	IC50	Eur J Med Chem (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL	Inhibition of rat brain recombinant nNOS	B	4.6	pIC50	25000	nM	IC50	Bioorg Med Chem (2008) 16: 5962-5973 [PMID:18502134]
ChEMBL	Inhibitory activity against rat neuronal nitric oxide synthase (nNOS)	B	5.67	pIC50	2120	nM	IC50	Bioorg Med Chem Lett (1999) 9: 2569-2572 [PMID:10498210]
ChEMBL	Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by neuronal NOS from rat cerebellum.	B	5.7	pIC50	2000	nM	IC50	Bioorg Med Chem Lett (1995) 5: 1573-1576
ChEMBL	In vitro inhibitory concentration against rat cerebellar neuronal nitric oxide synthase	B	6.1	pIC50	800	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1153-1156 [PMID:11354365]
ChEMBL	Inhibition of rat neuronal NOS	B	6.22	pIC50	600	nM	IC50	Bioorg Med Chem Lett (2007) 17: 3177-3180 [PMID:17395463]
ChEMBL	Inhibition of nNOS in Sprague-Dawley rat brain homogenates assessed as conversion of oxyhemoglobin to methemoglobin measured for 10 mins by UV-visible spectrophotometer analysis	B	6.96	pIC50	110	nM	IC50	Bioorg Med Chem (2011) 19: 3935-3944 [PMID:21665485]
Endothelial NOS/Nitric oxide synthase, endothelial in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075230] [GtoPdb: 1249] [UniProtKB: Q62600]
ChEMBL	Inhibition of nNOS in Wistar rat cortical homogenates incubated for 30 mins	B	6.15	pIC50	700	nM	IC50	Eur J Med Chem (2019) 168: 491-514 [PMID:30851693]
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
ChEMBL	Tested to inhibit the conversion of [3H]-L-arginine to [3H]L-citrulline catalyzed by endothelial NOS from human umbilical vein endothelial cells.	B	4.44	pIC50	36000	nM	IC50	Bioorg Med Chem Lett (1995) 5: 1573-1576
ChEMBL	In vitro inhibition of human endothelial nitric oxide synthase	B	5.6	pIC50	2500	nM	IC50	J Med Chem (2004) 47: 1575-1586 [PMID:14998342]
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase (eNOS)	B	5.72	pIC50	1890	nM	IC50	Bioorg Med Chem Lett (1999) 9: 2569-2572 [PMID:10498210]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Inhibition of bovine recombinant eNOS expressed in Escherichia coli assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay	B	4.39	pIC50	41000	nM	IC50	Eur J Med Chem (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL	Inhibition of bovine recombinant eNOS	B	4.46	pIC50	35000	nM	IC50	Bioorg Med Chem (2008) 16: 5962-5973 [PMID:18502134]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
ChEMBL	Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by inducible NOS from human DLD-1 cells.	B	4.62	pIC50	24000	nM	IC50	Bioorg Med Chem Lett (1995) 5: 1573-1576
ChEMBL	Inhibition of NOS2	B	5.24	pIC50	5800	nM	IC50	Bioorg Med Chem (2009) 17: 6180-6187 [PMID:19679481]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Inhibition of mouse recombinant iNOS	B	4.3	pIC50	50000	nM	IC50	Bioorg Med Chem (2008) 16: 5962-5973 [PMID:18502134]
ChEMBL	Inhibition of mouse recombinant iNOS expressed in Escherichia coli assessed as conversion of [gamma-14C]L-arginine to [14C]L-citrulline by radioactive assay	B	4.4	pIC50	40000	nM	IC50	Eur J Med Chem (2009) 44: 2877-2887 [PMID:19144449]
ChEMBL	Tested to inhibit the conversion of [3H]L-arginine to [3H]L-citrulline catalyzed by inducible NOS from mouse macrophage J774 cells	B	4.7	pIC50	20000	nM	IC50	Bioorg Med Chem Lett (1995) 5: 1573-1576

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]