ChEMBL ligand: CHEMBL107251
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Binding affinity nNOS	B	5.14	pKi	7300	nM	Ki	J Med Chem (2008) 51: 3661-3680 [PMID:18457385]
ChEMBL	Inhibition of recombinant human nNOS assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion in presence of NADPH	B	5.7	pKi	2000	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of recombinant human nNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis	B	5.11	pIC50	7800	nM	IC50	ACS Med Chem Lett (2020) 11: 1470-1475 [PMID:32676156]
ChEMBL	Inhibition of human nNOS assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 1 hr	B	5.7	pIC50	1980	nM	IC50	J Med Chem (2021) 64: 7760-7777 [PMID:34019417]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Inhibition of recombinant rat nNOS using [3H]L-arginine as substrate preincubated for 15 mins followed by NADPH addition measured after 20 mins in presence of FMN/FAD by liquid scintillation counting method	B	5.11	pIC50	7800	nM	IC50	Eur J Med Chem (2018) 152: 53-64 [PMID:29689474]
ChEMBL	Inhibition of rat brain nNOS assessed as nitric oxide mediated oxidation of oxyhemoglobin to methemoglobin	B	5.14	pIC50	7300	nM	IC50	Bioorg Med Chem Lett (2010) 20: 6495-6499 [PMID:20933416]
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
ChEMBL	Inhibition of recombinant human eNOS assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion in presence of NADPH	B	4.3	pKi	50000	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of human eNOS assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 1 hr	B	4.29	pIC50	51200	nM	IC50	J Med Chem (2021) 64: 7760-7777 [PMID:34019417]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Inhibition of full length N-terminal his-tagged bovine eNOS (2 to 1205 residues) expressed in Escherichia coli preincubated for 15 mins followed by NADPH addition and measured after 30 mins by HPLC analysis	B	4.3	pIC50	>50000	nM	IC50	Eur J Med Chem (2023) 248: 115112-115112 [PMID:36641860]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
ChEMBL	Inhibition of recombinant human iNOS assessed as reduction of L-[14C]arginine to L-[14C]citrulline conversion in presence of NADPH	B	8.15	pKd	7	nM	Kd	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of iNOS in LPS treated human THP1 cell homogenate assessed as conversion of L-[2,3-3H]arginine to L-[2,3-3H]citrulline pre-incubated 1 hr before LPS challenge by whole cell assay	B	6.48	pIC50	330	nM	IC50	J Med Chem (2009) 52: 1481-1485 [PMID:19203270]
ChEMBL	Inhibition of iNOS (unknown origin)	B	6.7	pIC50	200	nM	IC50	Eur J Med Chem (2023) 248: 115112-115112 [PMID:36641860]
ChEMBL	Inhibition of N-terminal his6-tagged recombinant human iNOS expressed in Escherichia coli preincubated for 15 mins followed by NADPH addition and measured after 30 mins by HPLC analysis	B	7.09	pIC50	81	nM	IC50	Eur J Med Chem (2023) 248: 115112-115112 [PMID:36641860]
ChEMBL	Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis	B	7.1	pIC50	80	nM	IC50	ACS Med Chem Lett (2020) 11: 1470-1475 [PMID:32676156]
ChEMBL	Inhibition of human iNOS assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 1 hr	B	8.08	pIC50	8.4	nM	IC50	J Med Chem (2021) 64: 7760-7777 [PMID:34019417]
GtoPdb	-	-	8.2	pIC50	6.31	nM	IC50	J Biol Chem (1997) 272: 4959-63 [PMID:9030556]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Inhibition of mouse macrophage iNOS assessed as nitric oxide mediated oxidation of oxyhemoglobin to methemoglobin	B	6.48	pIC50	330	nM	IC50	Bioorg Med Chem Lett (2010) 20: 6495-6499 [PMID:20933416]
ChEMBL	Inhibition of recombinant mouse iNOS using [3H]L-arginine as substrate preincubated for 15 mins followed by NADPH addition measured after 20 mins in presence of FMN/FAD by liquid scintillation counting method	B	7	pIC50	101	nM	IC50	Eur J Med Chem (2018) 152: 53-64 [PMID:29689474]
ChEMBL	Inhibition of mouse recombinant iNOS using [3H]L-arginine substrate assessed as formation of [3H]L-citruline by liquid scintillation counting analysis	B	7	pIC50	100	nM	IC50	ACS Med Chem Lett (2015) 6: 635-640 [PMID:26101565]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]