ChEMBL ligand: CHEMBL36 (Chloridin, Chloridine, Daraprim, GNF-PF-5586, Malacide, NSC-3061, Pyrimethamine, Pyrimethaminum, RP-4753, TCMDC-123831, TCMDC-125860, WR-2978)
Beta-hexosaminidase subunit alpha in Human [ChEMBL: CHEMBL1250415] [UniProtKB: P06865] There should be some charts here, you may need to enable JavaScript!
Beta-hexosaminidase subunit beta in Human [ChEMBL: CHEMBL5877] [UniProtKB: P07686] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum V1/S [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum K1 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum NF54 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase/Bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL4296323] [GtoPdb: 2981] [UniProtKB: Q8I1R6] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 7G8 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum D6 [GtoPdb: 2981] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4] Dihydrofolate reductase in Pneumocystis carinii [ChEMBL: CHEMBL1926] [UniProtKB: P16184] Dihydrofolate reductase in Candida albicans [ChEMBL: CHEMBL2329] [UniProtKB: P22906] Dihydrofolate reductase in Toxoplasma gondii [ChEMBL: CHEMBL2425] [UniProtKB: Q07422] Dihydrofolate reductase in Plasmodium falciparum K1 [ChEMBL: CHEMBL1939] [UniProtKB: P13922] dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] Dihydrofolate reductase in Bovine [ChEMBL: CHEMBL1075051] [UniProtKB: P00376] dihydrofolate reductase/Dihydrofolate reductase in Rat [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2] There should be some charts here, you may need to enable JavaScript!
Multidrug and toxin extrusion in Human [GtoPdb: 1216] [UniProtKB: Q96FL8] Multidrug and toxin extrusion/Multidrug and toxin extrusion protein 1 in Mouse [ChEMBL: CHEMBL3091264] [GtoPdb: 1216] [UniProtKB: Q8K0H1] There should be some charts here, you may need to enable JavaScript!
Plasmodium berghei [ChEMBL: CHEMBL612653] There should be some charts here, you may need to enable JavaScript!
Plasmodium cynomolgi [ChEMBL: CHEMBL613883] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum K1 [ChEMBL: CHEMBL612856] There should be some charts here, you may need to enable JavaScript!
Plasmodium yoelii [ChEMBL: CHEMBL612889] There should be some charts here, you may need to enable JavaScript!
Pteridine reductase 1 in Leishmania major [ChEMBL: CHEMBL6194] [UniProtKB: Q01782] There should be some charts here, you may need to enable JavaScript!
Organic cation transporter 1/Solute carrier family 22 member 1 in Human [ChEMBL: CHEMBL5685] [GtoPdb: 1019] [UniProtKB: O15245] Organic cation transporter 1/Solute carrier family 22 member 1 in Mouse [ChEMBL: CHEMBL2073664] [GtoPdb: 1019] [UniProtKB: O08966] There should be some charts here, you may need to enable JavaScript!
TAAR5/Trace amine-associated receptor 5 in Mouse [ChEMBL: CHEMBL3784908] [GtoPdb: 170] [UniProtKB: Q5QD14] There should be some charts here, you may need to enable JavaScript!
MATE2 in Mouse [GtoPdb: 1217] [UniProtKB: Q3V050] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Beta-hexosaminidase subunit alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1250415] [UniProtKB: P06865]
ChEMBL	Inhibition of HexA alpha G269S mutant in ATSD patient fibroblasts using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry	B	5.07	pIC50	8500	nM	IC50	J Med Chem (2015) 58: 4483-4493 [PMID:25984755]
Beta-hexosaminidase subunit beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5877] [UniProtKB: P07686]
ChEMBL	Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry	B	5.04	pIC50	9100	nM	IC50	J Med Chem (2015) 58: 4483-4493 [PMID:25984755]
ChEMBL	Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 7.0 by spectrofluorometry	B	5.35	pIC50	4500	nM	IC50	J Med Chem (2015) 58: 4483-4493 [PMID:25984755]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum V1/S [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	4	pIC50	>100000	nM	IC50	Proc Natl Acad Sci USA (2012) 109: 16823-8 [PMID:23035243]
GtoPdb	Parasite growth inhibition assay	-	5	pIC50	>10000	nM	IC50	PLoS Med (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum K1 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	5	pIC50	>10000	nM	IC50	PLoS Med (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum NF54 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	7.77	pIC50	17	nM	IC50	PLoS Med (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase/Bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4296323] [GtoPdb: 2981] [UniProtKB: Q8I1R6]
ChEMBL	Inhibition of pyrimethamine-resistant form-2 Plasmodium falciparum N51I, C59R, S108N, I164L, K96N mutant expressed in Escherichia coli BL21(DE3) by Michaelis-Menten based competitive inhibition assay	B	9.07	pKi	0.85	nM	Ki	Antimicrob Agents Chemother (2007) 51: 4356-4360 [PMID:17875995]
ChEMBL	Inhibition of pyrimethamine-resistant form-2 Plasmodium falciparum N51I, C59R, S108N, I164L, K96N mutant expressed in Escherichia coli BL21(DE3)	B	5.61	pIC50	2480	nM	IC50	Antimicrob Agents Chemother (2007) 51: 4356-4360 [PMID:17875995]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 7G8 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	5.17	pIC50	6688.7	nM	IC50	PLoS Med (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum D6 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	8.34	pIC50	4.6	nM	IC50	PLoS Med (2012) 9: e1001169 [PMID:22363211]
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1809] [UniProtKB: P0ABQ4]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46N Escherichia coli dihydrofolate reductase	B	7.8	pKd	16	nM	Kd	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for wild type Escherichia coli dihydrofolate reductase	B	7.89	pKd	13	nM	Kd	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46S Escherichia coli dihydrofolate reductase	B	8.82	pKd	1.5	nM	Kd	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46A Escherichia coli dihydrofolate reductase	B	8.85	pKd	1.4	nM	Kd	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibition constant against binding of Escherichia coli dihydrofolate reductase	B	6.55	pKi	281.84	nM	Ki	J Med Chem (1988) 31: 1396-1406 [PMID:3290487]
ChEMBL	Inhibitor constant of compound for mutant T46N Escherichia coli dihydrofolate reductase	B	7.7	pKi	20	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for wild type Escherichia coli dihydrofolate reductase	B	8	pKi	10	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for mutant T46S Escherichia coli dihydrofolate reductase	B	8.28	pKi	5.2	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for mutant T46A Escherichia coli dihydrofolate reductase	B	8.92	pKi	1.2	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibition of Escherichia coli DHFR	B	5.18	pIC50	6600	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	5.7	pIC50	2000	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
Dihydrofolate reductase in Pneumocystis carinii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1926] [UniProtKB: P16184]
ChEMBL	Binding affinity was reported with purified recombinant Pneumocystis carinii Dihydrofolate reductase	B	8.01	pKi	9.7	nM	Ki	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibition of Dihydrofolate reductase of Pneumocystis carinii	B	5.43	pIC50	3700	nM	IC50	J Med Chem (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibitory activity against Pneumocystis carinii Dihydrofolate reductase	B	5.43	pIC50	3700	nM	IC50	J Med Chem (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibition of Pneumocystis carinii Dihydrofolate Reductase	B	5.43	pIC50	3700	nM	IC50	J Med Chem (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	In vitro inhibitory concentration against Pneumocystis carinii dihydrofolate reductase	B	5.44	pIC50	3650	nM	IC50	J Med Chem (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase	B	5.44	pIC50	3650	nM	IC50	J Med Chem (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Pneumocystis carinii at 37 centigrade.	B	5.55	pIC50	2800	nM	IC50	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Compound was tested for inhibition activity against pneumocystis carinii (Pneumocystis carinii) Dihydrofolate reductase	B	5.62	pIC50	2400	nM	IC50	J Med Chem (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	5.62	pIC50	2400	nM	IC50	J Med Chem (2013) 56: 4422-4441 [PMID:23627352]
Dihydrofolate reductase in Candida albicans (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2329] [UniProtKB: P22906]
ChEMBL	Inhibition of dihydrofolate reductase in Candida albicans (in vitro).	B	5.3	pIC50	5000	nM	IC50	J Med Chem (1995) 38: 3608-3616 [PMID:7658448]
Dihydrofolate reductase in Toxoplasma gondii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2425] [UniProtKB: Q07422]
ChEMBL	Compound was tested for inhibition activity against Toxoplasma gondii (Toxoplasma gondii) Dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J Med Chem (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J Med Chem (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against Toxoplasma gondii Dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J Med Chem (1995) 38: 745-752 [PMID:7877140]
ChEMBL	The ability to inhibit Toxoplasma gondii Dihydrofolate reductase was tested	B	6.41	pIC50	390	nM	IC50	J Med Chem (1999) 42: 1007-1017 [PMID:10090784]
ChEMBL	Inhibitory activity against dihydrofolate reductase DHFR in Toxoplasma gondii.	B	6.41	pIC50	390	nM	IC50	J Med Chem (2001) 44: 2555-2564 [PMID:11472209]
ChEMBL	Inhibition of Toxoplasma gondii Dihydrofolate Reductase	B	6.41	pIC50	390	nM	IC50	J Med Chem (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	Inhibitory concentration against Toxoplasma gondii dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J Med Chem (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibition of Dihydrofolate reductase of Toxoplasma gondii	B	6.41	pIC50	390	nM	IC50	J Med Chem (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibition of Toxoplasma gondii TS-DHFR expressed in Escherichia coli BL21 preincubated for 15 mins followed by addition of DHF as substrate and NADPH measured after 60 mins by resazurin/diaphorase coupled assay	B	6.64	pIC50	230	nM	IC50	ACS Med Chem Lett (2016) 7: 1124-1129 [PMID:27994750]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	6.7	pIC50	200	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Toxoplasma gondii DHFR-TS expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 60 mins by resazurin dye based diaphorase-coupled assay	B	6.86	pIC50	139	nM	IC50	J Med Chem (2019) 62: 1562-1576 [PMID:30624926]
ChEMBL	Inhibition of Toxoplasma gondii dihydrofolate reductase	B	7.1	pIC50	80	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
Dihydrofolate reductase in Plasmodium falciparum K1 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1939] [UniProtKB: P13922]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with quadruple (N51I + C59R + S108N + I164L) mutations	B	6.07	pKi	860	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (N51I+C59R+S108N+I164L DHFR) of Plasmodium falciparum	B	6.41	pKi	385	nM	Ki	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with triple (C59R + S108N + I164L) mutations	B	6.42	pKi	380	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (C59R+S108N+I164L DHFR) of Plasmodium falciparum	B	6.95	pKi	112	nM	Ki	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Binding affinity was evaluated as inhibition of mutant (C59R + S108N) Plasmodium falciparum DHFR-TS.	B	7.14	pKi	71.7	nM	Ki	J Med Chem (1998) 41: 1367-1370 [PMID:9554869]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (N51I+C59R+S108N DHFR) of Plasmodium falciparum	B	7.17	pKi	67.1	nM	Ki	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of the C59R+S108N mutant of dihydrofolate reductase (DHFR)	B	7.27	pKi	53.9	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition of the S108N mutant of dihydrofolate reductase (DHFR)	B	7.54	pKi	28.6	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with single A16V mutation	B	8.22	pKi	6	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with double (A16V + S108T) mutations	B	8.44	pKi	3.6	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibitor constant of compound for mutant S108 N Plasmodium falciparum in dihydrofolate reductase	B	8.7	pKi	2	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Binding affinity was evaluated as inhibition of recombinant wild type (WT) Plasmodium falciparum DHFR-TS.	B	8.82	pKi	1.5	nM	Ki	J Med Chem (1998) 41: 1367-1370 [PMID:9554869]
ChEMBL	Inhibition constant against wild-type PfDHFR (Plasmodium falciparum dihydrofolate reductase)	B	8.82	pKi	1.5	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with single S108T mutation	B	8.85	pKi	1.4	nM	Ki	J Med Chem (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against Plasmodium falciparum dihydrofolate reductase	B	9.06	pKi	0.87	nM	Ki	J Med Chem (2004) 47: 4258-4267 [PMID:15293997]
ChEMBL	Binding affinity towards wild-type dihydrofolate reductase of Plasmodium falciparum.	B	9.22	pKi	0.6	nM	Ki	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of the wild-type dihydrofolate reductase (DHFR)	B	9.22	pKi	0.6	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibitor constant of compound for Plasmodium falciparum dihydrofolate reductase	B	9.72	pKi	0.19	nM	Ki	J Med Chem (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase CN51I/C59R/S108N/I164L (V1/S) mutant	F	4	pIC50	>100000	nM	IC50	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase N51I/C59R/S108N (W2)	F	4.13	pIC50	73500	nM	IC50	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase C59R+S108N/I164L (Csl-2) mutant	F	4.38	pIC50	41700	nM	IC50	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Antiplasmodial activity IC50 against Plasmodium falciparum K1CB1 DHFR double-mutant (C59R/S10)	F	4.51	pIC50	30900	nM	IC50	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase wild type (TM4/8.2)	F	7.1	pIC50	80	nM	IC50	J Med Chem (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of Plasmodium falciparum DHFR	B	7.1	pIC50	80	nM	IC50	Bioorg Med Chem Lett (2006) 16: 4366-4370 [PMID:16750361]
ChEMBL	Inhibition of Plasmodium falciparum DHFR	B	7.24	pIC50	58	nM	IC50	Bioorg Med Chem (2017) 25: 6467-6478 [PMID:29111368]
ChEMBL	Inhibition of Plasmodium falciparum DHFR using DHF as substrate preincubated for 15 mins followed DHF addition measured after 15 mins by spectrophotometric method	B	7.59	pIC50	25.5	nM	IC50	Bioorg Med Chem (2017) 25: 6467-6478 [PMID:29111368]
dihydrofolate reductase/Dihydrofolate reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374]
ChEMBL	Inhibition of human DHFR using dihydrofolate as substrate after 180 secs by spectrophotometric analysis	B	6.33	pKi	470	nM	Ki	Eur J Med Chem (2017) 135: 467-478 [PMID:28477572]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Leishmania major.	B	6.6	pKi	250	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	6.92	pKi	120	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Trypanosoma cruzi.	B	7.01	pKi	98	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Binding affinity to human recombinant DHFR expressed in Escherichia coli BL21(DE3) by competitive binding assay	B	7.51	pKi	30.8	nM	Ki	Antimicrob Agents Chemother (2010) 54: 2603-2610 [PMID:20350951]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	7.96	pKi	11	nM	Ki	J Med Chem (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Cytotoxicity by selective inhibition against human dihydrofolate reductase (DHFR).	B	8.1	pKi	8	nM	Ki	J Med Chem (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition of human dihydrofolate reductase	B	5.22	pIC50	6000	nM	IC50	J Med Chem (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibition of human DHFR	B	5.22	pIC50	6000	nM	IC50	Bioorg Med Chem (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	In vitro inhibition of human dihydrofolate reductase	B	5.59	pIC50	2600	nM	IC50	J Med Chem (1995) 38: 3608-3616 [PMID:7658448]
ChEMBL	Inhibition of human recombinant DHFR	B	6.4	pIC50	400	nM	IC50	J Med Chem (2013) 56: 4422-4441 [PMID:23627352]
Dihydrofolate reductase in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075051] [UniProtKB: P00376]
ChEMBL	Inhibition of bovine liver DHFR	B	7	pIC50	100.7	nM	IC50	Bioorg Med Chem (2017) 25: 5396-5406 [PMID:28789907]
ChEMBL	Inhibition of bovine liver DHFR pre-incubated 2 mins before dihydrofolic acid substrate addition and measured over 10 mins in presence of NADPH	B	7	pIC50	100.7	nM	IC50	Bioorg Med Chem (2017) 25: 4064-4075 [PMID:28634040]
dihydrofolate reductase/Dihydrofolate reductase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2]
ChEMBL	Inhibitory activity against dihydrofolate reductase in rat.	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibition of rat liver Dihydrofolate Reductase	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	In vitro inhibitory concentration against rat liver dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibition of Dihydrofolate reductase (DHFR) of in rat liver	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibitory activity against rat liver dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against rat liver Dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J Med Chem (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibitory activity against dihydrofolate reductase DHFR in rat liver	B	5.64	pIC50	2300	nM	IC50	J Med Chem (2001) 44: 2555-2564 [PMID:11472209]
ChEMBL	Compound was tested for inhibition activity against rat liver lipophilic Dihydrofolate reductase (DHFR).	B	5.82	pIC50	1500	nM	IC50	J Med Chem (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibition of rat liver DHFR