ChEMBL ligand: CHEMBL36 (Malacide, TCMDC-123831, GNF-PF-5586, Chloridin, Daraprim, Pyrimethamine, TCMDC-125860, WR-2978, NSC-3061, RP-4753)
Beta-hexosaminidase subunit alpha in Human [ChEMBL: CHEMBL1250415] [UniProtKB: P06865] There should be some charts here, you may need to enable JavaScript!
Beta-hexosaminidase subunit beta in Human [ChEMBL: CHEMBL5877] [UniProtKB: P07686] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum V1/S [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum K1 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum NF54 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase/Bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL4296323] [GtoPdb: 2981] [UniProtKB: Q8I1R6] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 7G8 [GtoPdb: 2981] Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum D6 [GtoPdb: 2981] There should be some charts here, you may need to enable JavaScript!
Dihydrofolate reductase in Escherichia coli [ChEMBL: CHEMBL5441] [UniProtKB: B0BL08] Dihydrofolate reductase in Pneumocystis carinii [ChEMBL: CHEMBL1926] [UniProtKB: P16184] Dihydrofolate reductase in Candida albicans [ChEMBL: CHEMBL2329] [UniProtKB: P22906] Dihydrofolate reductase in Toxoplasma gondii [ChEMBL: CHEMBL2425] [UniProtKB: Q07422] Dihydrofolate reductase in Plasmodium falciparum K1 [ChEMBL: CHEMBL1939] [UniProtKB: P13922] dihydrofolate reductase/Dihydrofolate reductase in Human [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374] Dihydrofolate reductase in Bovine [ChEMBL: CHEMBL1075051] [UniProtKB: P00376] dihydrofolate reductase/Dihydrofolate reductase in Rat [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2] There should be some charts here, you may need to enable JavaScript!
Multidrug and toxin extrusion in Human [GtoPdb: 1216] [UniProtKB: Q96FL8] Multidrug and toxin extrusion/Multidrug and toxin extrusion protein 1 in Mouse [ChEMBL: CHEMBL3091264] [GtoPdb: 1216] [UniProtKB: Q8K0H1] There should be some charts here, you may need to enable JavaScript!
Plasmodium berghei [ChEMBL: CHEMBL612653] There should be some charts here, you may need to enable JavaScript!
Plasmodium cynomolgi [ChEMBL: CHEMBL613883] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum 3D7 [ChEMBL: CHEMBL2366922] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum D6 [ChEMBL: CHEMBL2367107] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum (isolate K1 / Thailand) in Plasmodium falciparum K1 [ChEMBL: CHEMBL612856] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum NF54 [ChEMBL: CHEMBL2367131] There should be some charts here, you may need to enable JavaScript!
Plasmodium yoelii [ChEMBL: CHEMBL612889] There should be some charts here, you may need to enable JavaScript!
Organic cation transporter 1/Solute carrier family 22 member 1 in Human [ChEMBL: CHEMBL5685] [GtoPdb: 1019] [UniProtKB: O15245] Organic cation transporter 1/Solute carrier family 22 member 1 in Mouse [ChEMBL: CHEMBL2073664] [GtoPdb: 1019] [UniProtKB: O08966] There should be some charts here, you may need to enable JavaScript!
TAAR5/Trace amine-associated receptor 5 in Mouse [ChEMBL: CHEMBL3784908] [GtoPdb: 170] [UniProtKB: Q5QD14] There should be some charts here, you may need to enable JavaScript!
MATE2 in Mouse [GtoPdb: 1217] [UniProtKB: Q3V050] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Beta-hexosaminidase subunit alpha in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1250415] [UniProtKB: P06865]
ChEMBL	Inhibition of HexA alpha G269S mutant in ATSD patient fibroblasts using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry	B	5.07	pIC50	8500	nM	IC50	J. Med. Chem. (2015) 58: 4483-4493 [PMID:25984755]
Beta-hexosaminidase subunit beta in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5877] [UniProtKB: P07686]
ChEMBL	Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 4.5 by spectrofluorometry	B	5.04	pIC50	9100	nM	IC50	J. Med. Chem. (2015) 58: 4483-4493 [PMID:25984755]
ChEMBL	Inhibition of human placental HexB using MUGS substrate incubated for 1 to 2 hrs at pH 7.0 by spectrofluorometry	B	5.35	pIC50	4500	nM	IC50	J. Med. Chem. (2015) 58: 4483-4493 [PMID:25984755]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum V1/S [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	4	pIC50	>100000	nM	IC50	Proc. Natl. Acad. Sci. U.S.A. (2012) 109: 16823-8 [PMID:23035243]
GtoPdb	Parasite growth inhibition assay	-	5	pIC50	>10000	nM	IC50	PLoS Med. (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum K1 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	5	pIC50	>10000	nM	IC50	PLoS Med. (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum NF54 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	7.77	pIC50	17	nM	IC50	PLoS Med. (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase/Bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4296323] [GtoPdb: 2981] [UniProtKB: Q8I1R6]
ChEMBL	Inhibition of pyrimethamine-resistant form-2 Plasmodium falciparum N51I, C59R, S108N, I164L, K96N mutant expressed in Escherichia coli BL21(DE3) by Michaelis-Menten based competitive inhibition assay	B	9.07	pKi	0.85	nM	Ki	Antimicrob. Agents Chemother. (2007) 51: 4356-4360 [PMID:17875995]
ChEMBL	Inhibition of pyrimethamine-resistant form-2 Plasmodium falciparum N51I, C59R, S108N, I164L, K96N mutant expressed in Escherichia coli BL21(DE3)	B	5.61	pIC50	2480	nM	IC50	Antimicrob. Agents Chemother. (2007) 51: 4356-4360 [PMID:17875995]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum 7G8 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	5.17	pIC50	6688.7	nM	IC50	PLoS Med. (2012) 9: e1001169 [PMID:22363211]
Plasmodium falciparum bifunctional dihydrofolate reductase-thymidylate synthase in Plasmodium falciparum D6 [GtoPdb: 2981]
GtoPdb	Parasite growth inhibition assay	-	8.34	pIC50	4.6	nM	IC50	PLoS Med. (2012) 9: e1001169 [PMID:22363211]
Dihydrofolate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5441] [UniProtKB: B0BL08]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46N Escherichia coli dihydrofolate reductase	B	7.8	pKd	16	nM	Kd	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for wild type Escherichia coli dihydrofolate reductase	B	7.89	pKd	13	nM	Kd	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46S Escherichia coli dihydrofolate reductase	B	8.82	pKd	1.5	nM	Kd	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Thermodynamic dissociation constant of compound for mutant T46A Escherichia coli dihydrofolate reductase	B	8.85	pKd	1.4	nM	Kd	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for mutant T46N Escherichia coli dihydrofolate reductase	B	7.7	pKi	20	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for wild type Escherichia coli dihydrofolate reductase	B	8	pKi	10	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for mutant T46S Escherichia coli dihydrofolate reductase	B	8.28	pKi	5.2	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibitor constant of compound for mutant T46A Escherichia coli dihydrofolate reductase	B	8.92	pKi	1.2	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Inhibition of Escherichia coli DHFR	B	5.18	pIC50	6600	nM	IC50	Bioorg. Med. Chem. (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Escherichia coli dihydrofolate reductase	B	5.7	pIC50	2000	nM	IC50	J. Med. Chem. (2008) 51: 4589-4600 [PMID:18605720]
Dihydrofolate reductase in Pneumocystis carinii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1926] [UniProtKB: P16184]
ChEMBL	Binding affinity was reported with purified recombinant Pneumocystis carinii Dihydrofolate reductase	B	8.01	pKi	9.7	nM	Ki	J. Med. Chem. (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibition of Dihydrofolate reductase of Pneumocystis carinii	B	5.43	pIC50	3700	nM	IC50	J. Med. Chem. (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibitory activity against Pneumocystis carinii Dihydrofolate reductase	B	5.43	pIC50	3700	nM	IC50	J. Med. Chem. (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibition of Pneumocystis carinii Dihydrofolate Reductase	B	5.43	pIC50	3700	nM	IC50	J. Med. Chem. (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	In vitro inhibitory concentration against Pneumocystis carinii dihydrofolate reductase	B	5.44	pIC50	3650	nM	IC50	J. Med. Chem. (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibitory activity against Pneumocystis carinii dihydrofolate reductase	B	5.44	pIC50	3650	nM	IC50	J. Med. Chem. (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against dihydrofolate reductase in Pneumocystis carinii at 37 centigrade.	B	5.55	pIC50	2800	nM	IC50	J. Med. Chem. (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Compound was tested for inhibition activity against pneumocystis carinii (Pneumocystis carinii) Dihydrofolate reductase	B	5.62	pIC50	2400	nM	IC50	J. Med. Chem. (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibition of Pneumocystis carinii DHFR	B	5.62	pIC50	2400	nM	IC50	J. Med. Chem. (2013) 56: 4422-4441 [PMID:23627352]
Dihydrofolate reductase in Candida albicans (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2329] [UniProtKB: P22906]
ChEMBL	Inhibition of dihydrofolate reductase in Candida albicans (in vitro).	B	5.3	pIC50	5000	nM	IC50	J. Med. Chem. (1995) 38: 3608-3616 [PMID:7658448]
Dihydrofolate reductase in Toxoplasma gondii (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2425] [UniProtKB: Q07422]
ChEMBL	Compound was tested for inhibition activity against Toxoplasma gondii (Toxoplasma gondii) Dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibitory activity against Toxoplasma gondii dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against Toxoplasma gondii Dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1995) 38: 745-752 [PMID:7877140]
ChEMBL	The ability to inhibit Toxoplasma gondii Dihydrofolate reductase was tested	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1999) 42: 1007-1017 [PMID:10090784]
ChEMBL	Inhibitory activity against dihydrofolate reductase DHFR in Toxoplasma gondii.	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (2001) 44: 2555-2564 [PMID:11472209]
ChEMBL	Inhibition of Toxoplasma gondii Dihydrofolate Reductase	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	Inhibitory concentration against Toxoplasma gondii dihydrofolate reductase	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibition of Dihydrofolate reductase of Toxoplasma gondii	B	6.41	pIC50	390	nM	IC50	J. Med. Chem. (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibition of Toxoplasma gondii TS-DHFR expressed in Escherichia coli BL21 preincubated for 15 mins followed by addition of DHF as substrate and NADPH measured after 60 mins by resazurin/diaphorase coupled assay	B	6.64	pIC50	230	nM	IC50	ACS Med Chem Lett (2016) 7: 1124-1129 [PMID:27994750]
ChEMBL	Inhibition of Toxoplasma gondii DHFR	B	6.7	pIC50	200	nM	IC50	Bioorg. Med. Chem. (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	Inhibition of Toxoplasma gondii dihydrofolate reductase	B	7.1	pIC50	80	nM	IC50	J. Med. Chem. (2008) 51: 4589-4600 [PMID:18605720]
Dihydrofolate reductase in Plasmodium falciparum K1 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1939] [UniProtKB: P13922]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with quadruple (N51I + C59R + S108N + I164L) mutations	B	6.07	pKi	860	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (N51I+C59R+S108N+I164L DHFR) of Plasmodium falciparum	B	6.41	pKi	385	nM	Ki	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with triple (C59R + S108N + I164L) mutations	B	6.42	pKi	380	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (C59R+S108N+I164L DHFR) of Plasmodium falciparum	B	6.95	pKi	112	nM	Ki	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Binding affinity was evaluated as inhibition of mutant (C59R + S108N) Plasmodium falciparum DHFR-TS.	B	7.14	pKi	71.7	nM	Ki	J. Med. Chem. (1998) 41: 1367-1370 [PMID:9554869]
ChEMBL	Binding affinity towards mutant dihydrofolate reductase (N51I+C59R+S108N DHFR) of Plasmodium falciparum	B	7.17	pKi	67.1	nM	Ki	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of the C59R+S108N mutant of dihydrofolate reductase (DHFR)	B	7.27	pKi	53.9	nM	Ki	J. Med. Chem. (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition of the S108N mutant of dihydrofolate reductase (DHFR)	B	7.54	pKi	28.6	nM	Ki	J. Med. Chem. (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with single A16V mutation	B	8.22	pKi	6	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with double (A16V + S108T) mutations	B	8.44	pKi	3.6	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibitor constant of compound for mutant S108 N Plasmodium falciparum in dihydrofolate reductase	B	8.7	pKi	2	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	Binding affinity was evaluated as inhibition of recombinant wild type (WT) Plasmodium falciparum DHFR-TS.	B	8.82	pKi	1.5	nM	Ki	J. Med. Chem. (1998) 41: 1367-1370 [PMID:9554869]
ChEMBL	Inhibition constant against wild-type PfDHFR (Plasmodium falciparum dihydrofolate reductase)	B	8.82	pKi	1.5	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against PfDHFR (Plasmodium falciparum dihydrofolate reductase) with single S108T mutation	B	8.85	pKi	1.4	nM	Ki	J. Med. Chem. (2003) 46: 2834-2845 [PMID:12825927]
ChEMBL	Inhibition constant against Plasmodium falciparum dihydrofolate reductase	B	9.06	pKi	0.87	nM	Ki	J. Med. Chem. (2004) 47: 4258-4267 [PMID:15293997]
ChEMBL	Binding affinity towards wild-type dihydrofolate reductase of Plasmodium falciparum.	B	9.22	pKi	0.6	nM	Ki	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of the wild-type dihydrofolate reductase (DHFR)	B	9.22	pKi	0.6	nM	Ki	J. Med. Chem. (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibitor constant of compound for Plasmodium falciparum dihydrofolate reductase	B	9.72	pKi	0.19	nM	Ki	J. Med. Chem. (1992) 35: 2912-2915 [PMID:1495020]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase CN51I/C59R/S108N/I164L (V1/S) mutant	F	4	pIC50	>100000	nM	IC50	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase N51I/C59R/S108N (W2)	F	4.13	pIC50	73500	nM	IC50	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase C59R+S108N/I164L (Csl-2) mutant	F	4.38	pIC50	41700	nM	IC50	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Antiplasmodial activity IC50 against Plasmodium falciparum K1CB1 DHFR double-mutant (C59R/S10)	F	4.51	pIC50	30900	nM	IC50	J. Med. Chem. (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	In vitro anti-plasmodial activity against Plasmodium falciparum dihydrofolate reductase wild type (TM4/8.2)	F	7.1	pIC50	80	nM	IC50	J. Med. Chem. (2004) 47: 673-680 [PMID:14736247]
ChEMBL	Inhibition of Plasmodium falciparum DHFR	B	7.1	pIC50	80	nM	IC50	Bioorg. Med. Chem. Lett. (2006) 16: 4366-4370 [PMID:16750361]
ChEMBL	Inhibition of Plasmodium falciparum DHFR	B	7.24	pIC50	58	nM	IC50	Bioorg Med Chem (2017) 25: 6467-6478 [PMID:29111368]
ChEMBL	Inhibition of Plasmodium falciparum DHFR using DHF as substrate preincubated for 15 mins followed DHF addition measured after 15 mins by spectrophotometric method	B	7.59	pIC50	25.5	nM	IC50	Bioorg Med Chem (2017) 25: 6467-6478 [PMID:29111368]
dihydrofolate reductase/Dihydrofolate reductase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL202] [GtoPdb: 2603] [UniProtKB: P00374]
ChEMBL	Inhibition of human DHFR using dihydrofolate as substrate after 180 secs by spectrophotometric analysis	B	6.33	pKi	470	nM	Ki	Eur J Med Chem (2017) 135: 467-478 [PMID:28477572]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Leishmania major.	B	6.6	pKi	250	nM	Ki	J. Med. Chem. (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	6.92	pKi	120	nM	Ki	J. Med. Chem. (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from Trypanosoma cruzi.	B	7.01	pKi	98	nM	Ki	J. Med. Chem. (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Binding affinity to human recombinant DHFR expressed in Escherichia coli BL21(DE3) by competitive binding assay	B	7.51	pKi	30.8	nM	Ki	Antimicrob. Agents Chemother. (2010) 54: 2603-2610 [PMID:20350951]
ChEMBL	Inhibition of recombinant Dihydrofolate reductase from humans.	B	7.96	pKi	11	nM	Ki	J. Med. Chem. (1999) 42: 4300-4312 [PMID:10543874]
ChEMBL	Cytotoxicity by selective inhibition against human dihydrofolate reductase (DHFR).	B	8.1	pKi	8	nM	Ki	J. Med. Chem. (2002) 45: 1244-1252 [PMID:11881993]
ChEMBL	Inhibition of human dihydrofolate reductase	B	5.22	pIC50	6000	nM	IC50	J. Med. Chem. (2008) 51: 4589-4600 [PMID:18605720]
ChEMBL	Inhibition of human DHFR	B	5.22	pIC50	6000	nM	IC50	Bioorg. Med. Chem. (2012) 20: 4217-4225 [PMID:22739090]
ChEMBL	In vitro inhibition of human dihydrofolate reductase	B	5.59	pIC50	2600	nM	IC50	J. Med. Chem. (1995) 38: 3608-3616 [PMID:7658448]
ChEMBL	Inhibition of human recombinant DHFR	B	6.4	pIC50	400	nM	IC50	J. Med. Chem. (2013) 56: 4422-4441 [PMID:23627352]
Dihydrofolate reductase in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075051] [UniProtKB: P00376]
ChEMBL	Inhibition of bovine liver DHFR	B	7	pIC50	100.7	nM	IC50	Bioorg Med Chem (2017) 25: 5396-5406 [PMID:28789907]
ChEMBL	Inhibition of bovine liver DHFR pre-incubated 2 mins before dihydrofolic acid substrate addition and measured over 10 mins in presence of NADPH	B	7	pIC50	100.7	nM	IC50	Bioorg Med Chem (2017) 25: 4064-4075 [PMID:28634040]
dihydrofolate reductase/Dihydrofolate reductase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2363] [GtoPdb: 2603] [UniProtKB: Q920D2]
ChEMBL	Inhibitory activity against dihydrofolate reductase in rat.	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1995) 38: 4739-4759 [PMID:7490723]
ChEMBL	Inhibition of rat liver Dihydrofolate Reductase	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1997) 40: 3694-3699 [PMID:9357537]
ChEMBL	In vitro inhibitory concentration against rat liver dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1997) 40: 1886-1893 [PMID:9191966]
ChEMBL	Inhibition of Dihydrofolate reductase (DHFR) of in rat liver	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1994) 37: 4522-4528 [PMID:7799402]
ChEMBL	Inhibitory activity against rat liver dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1996) 39: 1271-1280 [PMID:8632434]
ChEMBL	Inhibitory activity against rat liver Dihydrofolate reductase	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (1995) 38: 745-752 [PMID:7877140]
ChEMBL	Inhibitory activity against dihydrofolate reductase DHFR in rat liver	B	5.64	pIC50	2300	nM	IC50	J. Med. Chem. (2001) 44: 2555-2564 [PMID:11472209]
ChEMBL	Compound was tested for inhibition activity against rat liver lipophilic Dihydrofolate reductase (DHFR).	B	5.82	pIC50	1500	nM	IC50	J. Med. Chem. (1998) 41: 913-918 [PMID:9526565]
ChEMBL	Inhibition of rat liver DHFR	B	5.82	pIC50	1500	nM	IC50	J. Med. Chem. (2013) 56: 4422-4441 [PMID:23627352]
ChEMBL	In vitro inhibitory concentration against rat liver dihydrofolate reductase	B	5.85	pIC50	1400	nM	IC50	J. Med. Chem. (1989) 32: 2468-2474 [PMID:2810335]
Multidrug and toxin extrusion in Human [GtoPdb: 1216] [UniProtKB: Q96FL8]
GtoPdb	-	-	7.1	pKi	77	nM	Ki	J. Pharmacol. Exp. Ther. (2010) 333: 341-50 [PMID:20065018]
Multidrug and toxin extrusion/Multidrug and toxin extrusion protein 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3091264] [GtoPdb: 1216] [UniProtKB: Q8K0H1]
ChEMBL	Inhibition of mouse Mate1 transfected in HEK293 cells assessed as uptake of [14C]-TEA preincubated for 15 mins by liquid scintillation counting analysis	B	6.84	pKi	145	nM	Ki	Bioorg. Med. Chem. (2013) 21: 7584-7590 [PMID:24238901]