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ChEMBL ligand: CHEMBL29097 (MK-886) |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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5-lipoxygenase activating protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4550] [UniProtKB: P20292] | ||||||||
ChEMBL | Inhibition of FLAP in human whole blood assessed as inhibition of calcium ionophore A23187-induced LTB4 production preincubated for 15 mins by ELISA | B | 5.85 | pIC50 | 1400 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Inhibition of FLAP in human whole blood assessed as inhibition of calcium ionophore A23187-induced LTB4 production preincubated for 5 hrs by ELISA | B | 5.96 | pIC50 | 1100 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Inhibition of FLAP in human whole blood assessed as inhibition of calcium ionophore A23187-induced LTB4 production preincubated for 11 hrs by ELISA | B | 6 | pIC50 | 1000 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Inhibition of FLAP in human leukocytes assessed as decrease in ionophore A23187-induced LT biosynthesis after 10 mins | B | 6.99 | pIC50 | 102 | nM | IC50 | Medchemcomm (2018) 9: 212-225 [PMID:30108915] |
ChEMBL | Inhibition of FLAP in human peripheral leukocytes assessed as inhibition of calcium ionophore A23187-induced LTB4 production after 3 hr by ELISA in presence of 0.5% human serum albumin | B | 7.57 | pIC50 | 27 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Binding affinity to FLAP | B | 7.59 | pIC50 | 26 | nM | IC50 | J Med Chem (2009) 52: 4968-4972 [PMID:19719242] |
ChEMBL | Inhibition of FLAP | B | 7.59 | pIC50 | 26 | nM | IC50 | Bioorg Med Chem Lett (2010) 20: 1604-1609 [PMID:20144869] |
ChEMBL | Inhibition of FLAP | B | 7.59 | pIC50 | 26 | nM | IC50 | J Med Chem (2008) 51: 4059-4067 [PMID:18459759] |
ChEMBL | Inhibition of FLAP (unknown origin) | B | 7.59 | pIC50 | 26 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 907-911 [PMID:23266122] |
ChEMBL | Binding affinity of compound for 5-lipoxygenase activating protein protein by FLAP binding assay | B | 7.64 | pIC50 | 23 | nM | IC50 | J Med Chem (1996) 39: 2629-2654 [PMID:8709092] |
ChEMBL | The compound was tested for the inhibition of binding of [125I]- L- 691,831 binding to 5-lipoxygenase activating protein (FLAP) | B | 7.64 | pIC50 | 23 | nM | IC50 | Bioorg Med Chem Lett (1992) 2: 1395-1398 |
ChEMBL | Inhibition of 5-lipoxygenase activating protein using human leukocyte membrane preparations | B | 7.64 | pIC50 | 23 | nM | IC50 | J Med Chem (1995) 38: 4538-4547 [PMID:7473582] |
ChEMBL | Inhibition of FLAP in human peripheral leukocytes assessed as inhibition of calcium ionophore A23187-induced LTB4 production after 10 mins by ELISA in presence of 0.5% human serum albumin | B | 7.72 | pIC50 | 19 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Inhibition of FLAP in A23187-stimulated human neutrophils assessed as 5-LO product formation preincubated for 15 mins measured after 10 mins | B | 7.77 | pIC50 | 17 | nM | IC50 | Bioorg Med Chem (2012) 20: 3728-3741 [PMID:22607880] |
ChEMBL | Inhibition of FLAP in human peripheral leukocytes assessed as inhibition of calcium ionophore A23187-induced LTB4 production after 1 hr by ELISA in absence of human serum albumin | B | 8.24 | pIC50 | 5.7 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
ChEMBL | Antagonist activity at FLAP in human PMNC assessed as inhibition of A23187-induced LTB4 biosynthesis pre-incubated for 2 mins followed by addition of A23187 measured after 5 mins by radioimmunoassay | B | 8.6 | pIC50 | 2.5 | nM | IC50 | J Med Chem (2016) 59: 5970-5986 [PMID:26791385] |
ChEMBL | Inhibition of FLAP in human peripheral leukocytes assessed as inhibition of calcium ionophore A23187-induced LTB4 production after 5 mins by ELISA in absence of human serum albumin | B | 8.66 | pIC50 | 2.2 | nM | IC50 | J Med Chem (2011) 54: 8013-8029 [PMID:22059882] |
5-LOX/5-lipoxygenase/FLAP in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111402] [GtoPdb: 1385] [UniProtKB: P09917, P20292] | ||||||||
ChEMBL | Human whole blood was stimulated with calcium ionophore (A23187) and LTB 4 measured by enzyme immunoassay | F | 6.47 | pIC50 | 340 | nM | IC50 | Bioorg Med Chem Lett (1996) 6: 1547-1552 |
ChEMBL | Inhibition of calcium ionophore (A23187) stimulated LTB4 formation in human neutrophils | F | 7.52 | pIC50 | 30 | nM | IC50 | Bioorg Med Chem Lett (1996) 6: 1547-1552 |
5-LOX/Arachidonate 5-lipoxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917] | ||||||||
ChEMBL | Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 incubated for 15 mins prior to prewarming for 30 secs followed by CaCl2+ and arachidonic acid addition and measured after 10 mins by RP-HPLC analysis | B | 5.52 | pIC50 | 3000 | nM | IC50 | Eur J Med Chem (2018) 150: 876-899 [PMID:29597170] |
ChEMBL | Inhibition of 5-LO in PMNL cells | B | 7.05 | pIC50 | 90 | nM | IC50 | J Med Chem (2008) 51: 8068-8076 [PMID:19053751] |
ChEMBL | Inhibition of 5-lipoxygenase in A23187-stimulated human PMNL assessed as enzyme product formation preincubated 15 mins by RP-HPLC analysis in presence of exogenous arachidonic acid | B | 7.05 | pIC50 | 90 | nM | IC50 | Bioorg Med Chem (2011) 19: 3394-3401 [PMID:21570310] |
ChEMBL | Inhibition of 5-lipoxygenase-mediated 5(S)-H(p)ETE formation in fMLP-stimulated human PMNL incubated 10 mins prior to fMLP challenge | B | 7.05 | pIC50 | 90 | nM | IC50 | J Med Chem (2011) 54: 4490-4507 [PMID:21591611] |
ChEMBL | Inhibition of 5-lipoxygenase in A23187-stimulated human polymorphonuclear leukocytes using arachidonic acid as substrate incubated 15 mins prior to substrate addition measured after 10 mins by HPLC analysis | B | 7.05 | pIC50 | 90 | nM | IC50 | J Med Chem (2013) 56: 9031-9044 [PMID:24171493] |
ChEMBL | The compound was tested for inhibitory activity against LT synthesis in human polymorphonuclear leukocytes[PMNS] | B | 7.22 | pIC50 | 60 | nM | IC50 | J Med Chem (1992) 35: 2501-2524 [PMID:1635053] |
ChEMBL | Inhibition of Human 5-lipoxygenase | B | 8 | pIC50 | >10 | nM | IC50 | J Med Chem (1995) 38: 4538-4547 [PMID:7473582] |
5-LOX/Arachidonate 5-lipoxygenase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL312] [GtoPdb: 1385] [UniProtKB: P12527] | ||||||||
ChEMBL | The compound was tested for inhibitory activity against 5-lipoxygenase translocation inhibitor in rat RBL-2H3 cells | F | 8.3 | pIC50 | 5 | nM | IC50 | J Med Chem (1992) 35: 2501-2524 [PMID:1635053] |
Cyclooxygenase-1 in Sheep (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2949] [UniProtKB: P05979] | ||||||||
ChEMBL | Inhibition of ovine COX1 using arachidonic acid as substrate pretreated for 5 mins followed by substrate addition and measured after 5 mins by HPLC analysis | B | 5.1 | pIC50 | 8000 | nM | IC50 | Eur J Med Chem (2018) 153: 2-28 [PMID:29329790] |
ChEMBL | Inhibition of ovine COX-1 assessed as 12-HHT formation preincubated for 5 mins followed by addition of arachidonic acid as substrate measured after 5 mins by HPLC analysis | B | 5.1 | pIC50 | 8000 | nM | IC50 | J Med Chem (2016) 59: 5970-5986 [PMID:26791385] |
ChEMBL | Inhibition of ovine COX1 | B | 8 | pIC50 | >10 | nM | IC50 | J Med Chem (2008) 51: 8068-8076 [PMID:19053751] |
COX-2 /Cyclooxygenase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354] | ||||||||
ChEMBL | Inhibition of human recombinant COX2 in cell-free system by enzyme immunoassay | B | 4.24 | pIC50 | 58000 | nM | IC50 | Bioorg Med Chem Lett (2014) 24: 5418-5422 [PMID:25453800] |
ChEMBL | Inhibition of human recombinant COX2 | B | 8 | pIC50 | >10 | nM | IC50 | J Med Chem (2008) 51: 8068-8076 [PMID:19053751] |
Macrophage-expressed gene 1 protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3414409] [UniProtKB: Q2M385] | ||||||||
ChEMBL | Inhibition of mPGES1 in IL-1beta stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 synthase activity after 15 mins using PGH2 substrate by RP-HPLC method | B | 5.66 | pIC50 | 2200 | nM | IC50 | Eur J Med Chem (2015) 94: 132-139 [PMID:25765759] |
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364] | ||||||||
ChEMBL | Antiplasmodial activity against Plasmodium falciparum D10 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum Dd2 after 72 hrs by SYBR green assay | F | 4.9 | pIC50 | 12589.25 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum HB3 after 72 hrs by SYBR green assay | F | 5 | pIC50 | 10000 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum 3D7 after 72 hrs by SYBR green assay | F | 5.2 | pIC50 | 6309.57 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum 7G8 after 72 hrs by SYBR green assay | F | 5.2 | pIC50 | 6309.57 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum GB4 after 72 hrs by SYBR green assay | F | 5.2 | pIC50 | 6309.57 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
ChEMBL | Antiplasmodial activity against Plasmodium falciparum W2 after 72 hrs by SYBR green assay | F | 5.5 | pIC50 | 3162.28 | nM | IC50 | Nat Chem Biol (2009) 5: 765-771 [PMID:19734910] |
mPGES1/Prostaglandin E synthase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5658] [GtoPdb: 1377] [UniProtKB: O14684] | ||||||||
ChEMBL | Inhibition of mPGES-1 activity in IL-1beta stimulated human A549 cells by cell-free assay | B | 5.41 | pIC50 | 3900 | nM | IC50 | Bioorg Med Chem Lett (2021) 41: 127992-127992 [PMID:33775835] |
ChEMBL | Inhibition of mPGES1 (unknown origin) using PGH2 as substrate incubated for 30 mins prior to substrate addition by PGH2-coupled spectrophotometric analysis | B | 5.41 | pIC50 | 3900 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 75-80 [PMID:23218602] |
ChEMBL | Inhibition of microsomal PGES1 using PGH2 as substrate by RP-HPLC method | B | 5.49 | pIC50 | 3200 | nM | IC50 | Bioorg Med Chem Lett (2012) 22: 7335-7339 [PMID:23147075] |
ChEMBL | Inhibition of mPGES-1 expressed in LPS-stimulated human A549 cells mitochondrial fraction assessed as conversion of PGH2 to PGE2 | B | 5.52 | pIC50 | 3000 | nM | IC50 | Bioorg Med Chem Lett (2011) 21: 1329-1333 [PMID:21310608] |
ChEMBL | Inhibition of mPGES-1 in interleukin-1 beta-stimulated human A549 cells microsomal membranes assessed as reduction in conversion of PGH2 to PGE2 pre-incubated for 15 mins followed by PGH2 substrate addition measured after 1 min by RP-HPLC method | B | 5.57 | pIC50 | 2700 | nM | IC50 | J Nat Prod (2017) 80: 731-734 [PMID:28165233] |
ChEMBL | Inhibition of human recombinant microsomal PGES-1 expressed in freestyle 293-f cells assessed as conversion of PGH2 to PGE2 after 15 mins by enzyme immunoassay | B | 5.59 | pIC50 | 2600 | nM | IC50 | Bioorg Med Chem (2011) 19: 6077-6086 [PMID:21920764] |
ChEMBL | Inhibition of human A549 cell microsomal membrane-derived mPGES-1 assessed as reduction in conversion of PGH2 to PGE2 preincubated for 15 mins followed by PGH2 addition measured after 1 min by RP-HPLC analysis | B | 5.6 | pIC50 | 2500 | nM | IC50 | J Nat Prod (2017) 80: 699-706 [PMID:28240894] |
ChEMBL | Inhibition of mPGES1 | B | 5.62 | pIC50 | 2400 | nM | IC50 | J Med Chem (2008) 51: 8068-8076 [PMID:19053751] |
ChEMBL | Inhibition of mPGES-1 in IL-1beta-stimulated human A549 cell microsomal membranes assessed as reduction in PGE2 formation incubated for 15 mins using PGH2 substrate by RP-HPLC method | B | 5.62 | pIC50 | 2400 | nM | IC50 | Bioorg Med Chem (2015) 23: 4839-4845 [PMID:26088337] |
ChEMBL | Inhibition of microsomal PGES1 isolated from IL-1beta-stimulated human A549 cells preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis | B | 5.62 | pIC50 | 2400 | nM | IC50 | J Nat Prod (2014) 77: 1445-1451 [PMID:24844534] |
ChEMBL | Inhibition of mPGES-1 from human A549 cell microsomal membranes using PGH2 as substrate incubated 15 mins prior to substrate addition measured after 1 min by RP-HPLC analysis | B | 5.62 | pIC50 | 2400 | nM | IC50 | J Med Chem (2013) 56: 9031-9044 [PMID:24171493] |
ChEMBL | Inhibition of mPGES-1 in human IL-1beta-stimulated A549 cell microsomes assessed as reduction of PGE2 formation from PGH2 after 15 mins by RP-HPLC analysis | B | 5.62 | pIC50 | 2400 | nM | IC50 | J Med Chem (2011) 54: 4490-4507 [PMID:21591611] |
ChEMBL | Inhibition of mPGES-1 in human IL-1beta-stimulated A549 cell microsomes assessed as reduction of PGE2 formation from PGH2 after 15 mins | B | 5.62 | pIC50 | 2400 | nM | IC50 | Bioorg Med Chem (2011) 19: 3394-3401 [PMID:21570310] |
ChEMBL | Inhibition of mPGES-1 in human IL-1beta-stimulated A549 cell microsomes assessed as inhibition of PGE2 formation from PGH2 after 15 mins by RP-HPLC analysis | B | 5.64 | pIC50 | 2300 | nM | IC50 | J Med Chem (2012) 55: 8958-8962 [PMID:22992107] |
ChEMBL | Inhibition of mPGES1 in IL1-beta treated human A549 cell microsomal membrane assessed as residual enzyme activity after 1 min by measuring PGE2 level using RP-HPLC method | B | 5.68 | pIC50 | 2110 | nM | IC50 | J Med Chem (2009) 52: 4968-4972 [PMID:19719242] |
ChEMBL | Inhibition of PGES1 in human A549 cell microsome assessed as PGE2 formation by cell-free assay | B | 5.68 | pIC50 | 2100 | nM | IC50 | J Med Chem (2008) 51: 8068-8076 [PMID:19053751] |
ChEMBL | Inhibition of PGES-1 in IL-1beta stimulated human A549 cell microsomes using PGH2 as substrate assessed as suppression of PGE2 formation preincubated for 15 mins followed by substrate addition by reversed phase-HPLC analysis | B | 5.68 | pIC50 | 2100 | nM | IC50 | Bioorg Med Chem (2016) 24: 820-826 [PMID:26777299] |
ChEMBL | Inhibition of mPGES-1 activity in IL-1beta stimulated human A549 cells microsomes assessed as reduction in PGE2 production preincubated for 15 mins followed by PGH2 addition and measured after 1 mins by EIA | B | 5.68 | pIC50 | 2100 | nM | IC50 | Bioorg Med Chem Lett (2021) 41: 127992-127992 [PMID:33775835] |
ChEMBL | Inhibition of mPGES1 in IL-1beta treated human A549 cell microsomal membrane assessed as inhibition of PGE2 formation incubated for 15 mins before addition of PGH2 by RP-HPLC method | B | 5.68 | pIC50 | 2100 | nM | IC50 | J Nat Prod (2011) 74: 1779-1786 [PMID:21800856] |
ChEMBL | Inhibition of mPGES1 in human A549 cell microsomes | B | 5.68 | pIC50 | 2100 | nM | IC50 | Eur J Med Chem (2018) 153: 2-28 [PMID:29329790] |
ChEMBL | Inhibition of mPGES1 in human A549 cell microsomal membrane using pGH2 as substrate pretreated for 15 mins followed by substrate addition and measured after 1 min by RP-HPLC method | B | 5.68 | pIC50 | 2100 | nM | IC50 | Eur J Med Chem (2018) 153: 2-28 [PMID:29329790] |
ChEMBL | Inhibition of mPGES1 in IL1-beta induced human A549 cell microsomal membrane assessed as blockade of conversion of PGH2 to PGE2 | B | 5.7 | pIC50 | 2000 | nM | IC50 | Bioorg Med Chem (2009) 17: 7924-7932 [PMID:19884011] |
ChEMBL | Inhibition of PGES-1 in human A549 cell microsomes using PGH2 as substrate assessed as suppression of interleukin-1beta-stimulated PGE2 production incubated for 15 mins post interleukin-1beta challenge for 72 hrs by RP-HPLC analysis | B | 5.7 | pIC50 | 2000 | nM | IC50 | Bioorg Med Chem Lett (2016) 26: 94-99 [PMID:26602278] |
ChEMBL | Inhibition of microsomal PGES1 (unknown origin) | B | 5.8 | pIC50 | 1600 | nM | IC50 | Medchemcomm (2015) 6: 2081-2123 |
ChEMBL | Inhibition of human mPGES1 | B | 5.8 | pIC50 | 1600 | nM | IC50 | Bioorg Med Chem Lett (2010) 20: 1604-1609 [PMID:20144869] |
ChEMBL | Inhibition of mPGES-1 | B | 5.8 | pIC50 | 1600 | nM | IC50 | Eur J Med Chem (2012) 48: 153-163 [PMID:22209272] |
ChEMBL | Inhibition of human recombinant mPGES-1 using PGH2 as substrate incubated 20 mins prior to substrate addition measured after 30 secs by EIA | B | 5.8 | pIC50 | 1600 | nM | IC50 | Bioorg Med Chem Lett (2013) 23: 907-911 [PMID:23266122] |
ChEMBL | Inhibition of microsomal PGES | B | 5.8 | pIC50 | 1600 | nM | IC50 | Bioorg Med Chem (2011) 19: 6077-6086 [PMID:21920764] |
ChEMBL | Inhibition of human recombinant mPGES-1 in assessed as conversion of PGH2 into PGE2 at 20 degC after 5 mins by HPLC-UV analysis | B | 5.89 | pIC50 | 1300 | nM | IC50 | Eur J Med Chem (2012) 48: 153-163 [PMID:22209272] |
ChEMBL | Inhibition of microsomal PGES1 in human whole blood | B | 5.96 | pIC50 | 1100 | nM | IC50 | Medchemcomm (2015) 6: 2081-2123 |
ChEMBL | Inhibition of microsomal PGES-1 (unknown origin) | B | 8.52 | pIC50 | 3 | nM | IC50 | J Med Chem (2013) 56: 3296-3309 [PMID:23527738] |
mPGES2/Prostaglandin E synthase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4411] [GtoPdb: 1378] [UniProtKB: Q9H7Z7] | ||||||||
ChEMBL | Inhibitory concentration against human prostaglandin E2 synthase (mPGES-1) | B | 5.8 | pIC50 | 1600 | nM | IC50 | Bioorg Med Chem Lett (2005) 15: 3352-3355 [PMID:15953724] |
Peroxisome proliferator-activated receptor-α in Mouse [GtoPdb: 593] [UniProtKB: P23204] | ||||||||
GtoPdb | - | - | 6 | pIC50 | - | - | - | Biochem J (2001) 353: 253-8 [PMID:11139388] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]